This research was carried out to identify salt tolerant potato genotypes in vitro among 131 tetraploid potato cultivars (Solanum tuberosum), 9 diploid simple hybrid clones (4 clones of S. chacoense $ times$ S. tuberosum, 4 clones of S. phureja/S. stenotomum $ times$ S. tuberosum, and 1 clone of S. tuberosum $ times$ S. tuberosum), 1 primitive cultivated diploid S. phureja/S. stenotomum accession, 12 tetraploid complex hybrids, and 13 diploid S. chacoense accessions. Four levels of NaCl (0, 40, 80, and 120 mM) were used. The cultivars, and the simple and complex hybrids were tested for salt tolerance at the vegetative stage in the nodal cutting bioassay. The thirteen S. chacoense accessions were tested for salt tolerance at the germination and early seedling growth stage, in a seedling bioassay. Eleven of these S. chacoense accessions were further tested at the vegetative stage, in the nodal cutting bioassay. There was a progressive decline in the morphological parameters measured, with increased salt levels, in the nodal cutting bioassay. The parameters were used collectively in ranking the different genotypes, averaged over three NaCl levels (40, 80, and 120 mM). Twenty potato cultivars, two clones of the simple hybrid S. chacoense $ times$ S. tuberosum, and one complex hybrid were all considered salt tolerant at the vegetative stage. Ranking of seven S. chacoense accessions was similar between early seedling growth and later vegetative stage. Two of these accessions were promising as sources of salt tolerance.

Thesis (Ph.D.)--University of Western Australia, 2006.

No description available.

No description available.

Dryland salinity is a major and expanding threat to agricultural land in Australia. Animal production from forages grown on saline land is perhaps its most promising economic use. Glycophytic forage legumes have been evaluated under saline conditions mainly for agronomic characteristics and, to a lesser extent, for nutritive quality to animals. Plant growth and its nutritive quality are interrelated, but a decline in yield in response to salinity may be associated with effects on the chemical constituents of the plant since soil salinity affects plant metabolism. This research aimed to investigate changes in the components of yield and nutritive value of two legumes species. Lucerne ( Medicago sativa ) and Melilotus ( Melilotus albus ) were exposed to different levels of NaCl in the range of 0 to 110 mM NaCl. The research tested the hypothesis that the components of plant nutritive value are not as sensitive to salinity as shoot biomass production since the adaptive mechanisms of the plant lessen harmful effects of the salts. For both plant species, salinity decreased leaf and stem dry matter production, but increased leaf - to - stem ratio. In addition, salinity resulted in earlier flowering in Melilotus. Mineral composition was the most sensitive component of forage quality. Calculated sodium chloride concentrations were up to 125 g / kg DM in lucerne and 39 g / kg DM in Melilotus when irrigated with 110 mM NaCl. The concentrations of calcium and magnesium decreased in both species and approached the marginal range for animal production. Zinc concentration also decreased while potassium decreased in stems of lucerne only. The digestible organic matter ( DOMD ) in response to salinity varied between species. At the highest salt concentration, the whole shoot ( i.e., leaf and stem ) of lucerne decreased up to 4 percentage units while Melilotus increased by 6 percentage units. In lucerne, DOMD was influenced by a high concentration of soluble ash in leaf and stem and, in Melilotus, by an increase in the organic matter content of leaf and a reduction in lignin concentration in stem, which favoured higher digestibility. These results were supported by a histological study in which an increase in starch in Melilotus leaf, and a lower proportion of xylem in relation to parenchyma in stems, was measured. Crude protein concentration was not compromised and, in relation to Melilotus, coumarin concentration did not increase with salinity. In conclusion, the reduction in DM production of species with similar salt tolerance does not necessarily correspond to an equivalent reduction in nutritive value. This research represents the most detailed study into effects of salinity on glycophytic forage legumes. Results show that while some aspects of forage quality ( e.g., minerals composition and energy ) are strongly influenced by salinity, other aspects ( e.g., protein ) remain relatively unaffected. These findings have implications for development of productive grazing systems on saline agricultural land. / Thesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.

Dryland salinity is a major and expanding threat to agricultural land in Australia. Animal production from forages grown on saline land is perhaps its most promising economic use. Glycophytic forage legumes have been evaluated under saline conditions mainly for agronomic characteristics and, to a lesser extent, for nutritive quality to animals. Plant growth and its nutritive quality are interrelated, but a decline in yield in response to salinity may be associated with effects on the chemical constituents of the plant since soil salinity affects plant metabolism. This research aimed to investigate changes in the components of yield and nutritive value of two legumes species. Lucerne ( Medicago sativa ) and Melilotus ( Melilotus albus ) were exposed to different levels of NaCl in the range of 0 to 110 mM NaCl. The research tested the hypothesis that the components of plant nutritive value are not as sensitive to salinity as shoot biomass production since the adaptive mechanisms of the plant lessen harmful effects of the salts. For both plant species, salinity decreased leaf and stem dry matter production, but increased leaf - to - stem ratio. In addition, salinity resulted in earlier flowering in Melilotus. Mineral composition was the most sensitive component of forage quality. Calculated sodium chloride concentrations were up to 125 g / kg DM in lucerne and 39 g / kg DM in Melilotus when irrigated with 110 mM NaCl. The concentrations of calcium and magnesium decreased in both species and approached the marginal range for animal production. Zinc concentration also decreased while potassium decreased in stems of lucerne only. The digestible organic matter ( DOMD ) in response to salinity varied between species. At the highest salt concentration, the whole shoot ( i.e., leaf and stem ) of lucerne decreased up to 4 percentage units while Melilotus increased by 6 percentage units. In lucerne, DOMD was influenced by a high concentration of soluble ash in leaf and stem and, in Melilotus, by an increase in the organic matter content of leaf and a reduction in lignin concentration in stem, which favoured higher digestibility. These results were supported by a histological study in which an increase in starch in Melilotus leaf, and a lower proportion of xylem in relation to parenchyma in stems, was measured. Crude protein concentration was not compromised and, in relation to Melilotus, coumarin concentration did not increase with salinity. In conclusion, the reduction in DM production of species with similar salt tolerance does not necessarily correspond to an equivalent reduction in nutritive value. This research represents the most detailed study into effects of salinity on glycophytic forage legumes. Results show that while some aspects of forage quality ( e.g., minerals composition and energy ) are strongly influenced by salinity, other aspects ( e.g., protein ) remain relatively unaffected. These findings have implications for development of productive grazing systems on saline agricultural land. / Thesis (Ph.D.)--School of Agriculture, Food and Wine, 2006.

The possibility of manipulating calcium (Ca2+) and potassium (K+) levels in seeds of Brassica rapa by altering parent plant nutrition and investigating the potential for increased salinity tolerance during germination, given that considerable amounts of literature imply that greater amounts of available exogenous Ca2+ and K+ can ameliorate the effects of salinity on both whole plant growth and germination, was evaluated. The investigation consisted of four growth trials. Two preliminary growth trials suggested that seed ion manipulation was possible without affecting the overall growth and vigour of the plant. After developing suitable high and low Ca2+ and K+ nutrient solutions for growth, a trial was carried out in a growth room and greenhouse, with various substrates and the seed of a certain size category was collected for subsequent ion and salinity tolerance analysis. Seed Ca2+ and K+ was significantly affected by growth substrate and nutrient solution and data showed that a significant negative regression relationship existed between seed Ca2+, K+ and Ca2+ + K+ levels and salinity tolerance. Further experimentation using hydroponic culture attempted to remove any possible effects of substrate and also to compare size categories of seed with a view to elucidating localisation of Ca2+ and K+. Seed Ca2+ was found to be significantly altered by nutrient solution in the two different sizes tested and higher Ca2+ nutrient solution was found to increase salinity tolerance in daughter seed. One significant negative regression correlation between salinity tolerance and seed K+ concentration existed in smaller seed, but disregarding seed size in a regression analysis of seed ion content and salinity tolerance, a significant negative relationship existed between seed Ca2+, K+ and Ca2++ K+. The results, especially in terms of Ca2+ nutrition, contradict much previous research that suggests increased salinity tolerance at germination can arise with the increased presence of Ca2+ and/or K+. Salinity tolerance was greater in seeds of larger size across all nutritional treatments and the smaller size range exhibited increased Ca2+ and K+ per μg seed. Ca2+ concentration in smaller seeds with greater surface area:volume ratios provided a clue to the potential localisation of Ca2+. Cross sectional staining showed that a greater proportion of seed Ca2+ may reside in the coat. This was confirmed by analysis which showed an approximate 50% split of total extractable seed Ca2+, regardless of size, between coat and embryo within a seed; the majority of which, per μg, resides in the coat. Further work looked at the relative solubility of the Ca2+ and K+ in these tissues and whole seed to look at the potential bioavailability of Ca2+ during germination from various parts of the seed. Most water soluble Ca2+ exists in the embryo and most insoluble Ca2+ exists in the coat, but coat Ca2+ was found to be ionically exchangeable and therefore bioavailable. K+ appeared mostly water soluble in embryo and coat. In line with previous whole plant research in this species, most Ca2+ is readily water soluble or ionically exchangeable in form and the possible negative effects of how increasing bioavailable Ca2+ may reduce salinity tolerance was discussed.

In Libya salinization of land and ground water is a key problem. A. saligna is one species that offers potential for growth under these conditions. Experiments were undertaken to accelerate the germination of Acacia seeds, and various pre-treatment methods were assayed. Boiling water treatment, mechanical scarification and acid improved germination and germination rate. Sowing A. saligna seeds at 30 mm depth gave greatest seedling growth from large and medium seeds whereas 20 mm was more suitable for small seeds. This study aimed to improve the establishment of Acacia saligna irrigated with saline water, plants were grown for seven weeks under greenhouse or field conditions in (Libya) either sand or soil salinized with varying applications of NaCl. Irrigation with NaCl significantly decreased plant survival and growth and concentration of Ca, Na, K, Fe and P ions in plants with 0.5 M or 1.0 M NaCl. The experiments were repeated using a naturally occurring soil amendment called ‘Zander’. Seeds of A. saligna were grown for seven weeks in both greenhouse and field trials as before but with the addition of Zander and NaCl. Zander improved plant survival and growth with salinity and increased the elements in plants (Ca, Na, K, Fe and P). Field experiments were conducted to assess the effects of saline irrigation with 1.0 M NaCl and extra water added to 0% or 10% Zander on survival and growth, consequently, seedling growth significantly decreased with increase in soil salinity. Survival and growth increased with increase in extra water. The additional irrigation water caused an increase in the uptake of Ca++ and increased the Ca++/Na+ and K+/Na+ ratio. Zander did not appear to reduce net uptake of Na+ and its transport to shoot tissues. Mg++, P, K+ and Ca++ content significantly decreased in plants in response to salinity. Possible mechanisms to avoid Na+ toxicity in A. saligna in response to salinity included increasing the supply of Ca++. Extra Ca++ applied into the medium with and without salt increased survival and growth even in the absence of Zander. Calcium increased uptake of Ca++ and increased Ca++/Na+ and K+/Na+ ratio.

Thesis (MSc)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: Soil salinity is a major limiting factor of plant and crop growth, because the absorption of water and nutrients is such a complex process while low and moderate salinity are omnipresent. Plant growth is affected negatively if a specific ion concentration exceeds its threshold and becomes toxic. The detrimental effect of soil affected by salt on crop production is increasing worldwide (Tanji, 1990). The level to which plants can tolerate high salinity levels is genetically controlled with several physiological and genetic mechanisms contributing to salt tolerance (Epstein & Rains, 1987). The most effective way of addressing the limitations of crop productivity in saline areas, is the development of salt tolerant varieties. Understanding the genetics of salt tolerance is, therefore, necessary for the development of an effective breeding strategy for salt tolerance. The department of Genetics (US) conducts a wide crosses research programme aiming to transfer genes for salt tolerance to wheat and triticale. The donor species, Thinopyrum disticum, an indigenous coastal wheat grass, adapted to high concentrations of salt, was crossed with cultivated rye (Secale cereale) in an attempt to study the genetics of salt tolerance (Marais et al., 1998). The primary goal of this study was to find molecular markers (RAPD and AFLP) which associate with chromosomes promoting salt tolerance for later attempts to transfer the genes to triticale. Seventy clones of secondary hybrids (Th disticum /4x-rye 1/2x-rye) were tested for salt tolerance and showed different levels of salt tolerance. RAPD-marker analyses were used to identify polymorphisms between salt tolerant and salt sensitive plants. Twelve RAPD primers produced clear, analyzable and repetitive polymorphic . fragments that can be used as useful markers. Different AFLP-primer combinations were tested against the genotypes of 15 clones (Marais & Marais 2001, unpublished data) and produced approximately 2000 clearly distinguishable AFLP fragments, of which 54 (3%) were polymorphic fragments. Two RAPD fragments and 4 AFLP fragments that can be used as possible markers for the presence of chromosomes that contribute to salt tolerance were identified. The interpretation of the markers was complicated by heterogeneity among plants with regard to the origin of their chromosomes and the genetic diversity of the rye genome. It is also possible that chromosome re-arrangement took place during backcrossing, which could have complicated the data. / AFRIKAANSE OPSOMMING: Versouting is een van die groot beperkende faktore op plant- en gewasgroei, omdat die opname van water en voedingstowwe so In ingewikkelde proses is en die effek van lae of matige versouting so alomteenwoordig is. Plantgroei word nadelig geaffekteer as 'n spesifieke ioonkonsentrasie sy drempelwaarde oorskry en toksies word. Die nadelige effek van soutgeaffekteerde grond op gewasproduksie, is wêreldwyd aan die toeneem (Tanji, 1990). Die vlak waartoe plante hoë konsentrasies sout kan hanteer is onder genetiese beheer met verskeie fisiologiese en genetiese meganismes wat 'n bydrae maak tot soutverdraagsaamheid (Epstein & Rains, 1987). Die mees effektiewe manier om die beperkinge op gewas produktiwiteit in versoute gebiede te oorkom, is die ontwikkeling van soutverdraagsame variëteite. Begrip van die genetika van soutverdraagsaamheid is dus noodsaaklik vir die ontwikkeling van In effektiewe telingsstrategie. Die departement Genetika (US) bedryf tans 'n wye-kruisings navorsingsprogram waarmee gepoog word om gene vir soutverdraagsaamheid na korog en koring oor te dra. Die skenkerspesie, Thinopyrum disticum, In inheemse strandkoringgras wat aangepas is by hoë konsentrasies sout, is gekruis met verboude rog (Secale cereale) in 'n poging om die oorerwing van soutverdraagsaamheid te bestudeer (Marais et al., 1998). Die hoofdoel van hierdie studie was om molekulêre merkers (RAPD en AFLP) te vind, wat assosieer met chromosome wat soutverdraagsaamheid bevorder en om nuttige merkers daar te stel vir latere pogings om die gene na korog en koring oor te dra. Ongeveer 70 klone van sekondêre hibriede (Th distichum I 4x-rog /I 2x-rog) is onderwerp aan souttoetse en het verskillende grade van soutverdraagsaamheid getoon. RAPDmerker analise is gebruik om polimorfismes te identifiseer tussen soutverdraagsame en soutsensitiewe plante. Twaalf RAPD inleiers het duidelike, ontleedbare en herhalende polimorfiese fragmente opgelewer en moontlike nuttige merkers uitgewys. Verskillende AFLP-inleier kombinasies, wat getoets is teen die genotipes van 15 klone (Marais & Marais, 2001 ongepubliseerde data) het ongeveer 2000 duidelik onderskeibare AFLP fragmente geproduseer, waarvan 54 (3%) polimorfiese fragmente was. Twee RAPD fragmente en 4 AFLP fragmente is geïdentifiseer wat as moontlike kandidaat merkers gebruik kan word vir die identifisering van chromosome wat bydra tot soutverdraagsaamheid . Die interpretasie van die merkers is bemoeilik deur heterogeniteit tussen die plante wat betref die agtergrond van chromosome wat hulle besit en die genetiese diversiteit van die rog genoom. Dit is ook moontlik dat chromosoom herrangskikking plaasgevind het tydens terugkruising, wat die data verder kon kompliseer.

Two accessions of tepary (phaseolus acutifolius Gray var. latifolius) and navy (P. vulgaris L. 'Fleetwood') beans were studied for salt tolerance at several• developmental stages. Genotypes were germinated at 0.0 through -2.5 MPa NaCl at 25°C and 35°C for nine days. Tepary accessions had higher germination percentages and rates than navy for ≤ - 2.0 MPa at 250C and ≤ - 1.5 MPa at 35°C. Fresh weights of root plus hypocotyl decreased severely with the first increment of NaCl (-0.5 MPa) for all genotypes. Fresh weight of navy was reduced more at 35°C than at 25°C. Genotypes were stressed in vermiculite-filled trays with 0.0 through -1.5 MPa NaCl for 14 days. Final growth stage and rates of emergence were reduced at salinities ~ -0.6 MPa NaCl, and were higher in tepary than navy at -1.2 MPa. Tepary beans tended to maintain higher water and osmotic potentials, and at -0.9 MPa had less reduction in leaf area than navy beans. Fresh weights, dry weights and root:shoot ratios declined in all genotypes with increasing salinities. Plants grown hydroponically were stressed with -0.10, -0.25, and -0.50 MPa NaCl during either vegetative or reproductive stages. Navy had equal or greater fresh and dry weights of leaf, stem, and pods at -0.10 MPa, but tepary beans had equal or greater weights at the highest salinity relative to navy. Tepary had the greatest pod weight with -0.50 MPa NaCl applied during the reproductive stage. Carbon exchange rates (CER) were lower in navy than one or both tepary beans at some sampling times. Tepary beans tended to have higher leaf water and osmotic potentials than did navy. Transpiration and stomatal resistance values were similar in all genotypes, while leaf temperatures were different in white tepary versus navy. Tepary beans yielded higher than navy when grown in low and high salinity fields. Transpiration rates, leaf water and osmotic potentials, and CERs were similar or higher, while stomatal resistance and leaf temperatures were similar or lower in tepary than in navy. Plant height and stand count also were measured. Tepary was more salt tolerant than navy, exhibiting greater tolerance to NaCl at every growth stage.