Bibliography: pages 95-99. / The aim of this project was to characterise an early histone gene battery isolated from Parenchinus angulosus. An early histone gene battery (named H27) which was believed to have been isolated from Parenchinus angulosus, appeared by restriction enzyme mapping and partial sequencing to be identical to H22, an early histone gene battery isolated from Psammechinus miliaris. (This latter gene was obtained from M. Birnstiel.) This was further confirmed by electron microscopy, and proved to be a convenient testing ground for the electron microscopic techniques of denaturation mapping and heteroduplex anlysis. Another gene battery (named SU1) isolated fromParenchinus angulosus, was then characterised using the techniques developed whilst studying H27. The restriction enzyme map of this clone is different to that of H22, indicating that differences do indeed exist between these two early histone gene batteries. SU1 also showed the expected order of the five histone genes, as determined by hybridization against the coding regions of H22. The denaturation map of SU1 showed AT rich spacer regions and GC rich coding regions. Heteroduplex analysis indicated that the spacer regions between the Hl and H2A, the H2A and H3, and the H3 and the H2B gene coding areas are essentially nonhomologous. The H4 structural gene and corresponding spacer regions were not included in this analysis. Because it is known that all the five histones are coded for on the same strand of DNA in H22, and that each of the genes is transcribed in the same direction, it follows that, the same holds for, at least, the Hl, H2A, H3 and H2B genes of SU1.

No description available.

Thesis (M.S.)--California Polytechnic State University, 2009. / Title from PDF title page; viewed on October 23, 2009. Major professor: Nikki L. Adams. "Presented to the faculty of California Polytechnic State University, San Luis Obispo." "In partial fulfillment of the requirements for the degree [of] Master of Science in Biological Sciences." "June 2009." Includes bibliographical references (p. ). Also available on microfiche.

Thesis (Ph. D.)--San Diego State University, and University of California, Davis, 2003. / Includes bibliographical references (leaves 65-72). Also available via the World Wide Web. (Restricted to UC campuses)

Thesis (Ph. D.)--Duke University, 2007. / Includes bibliographical references.

The monoclonal antibody Sp12 recognizes an epitope shared by a diverse group of developmentally regulated cell surface and extracellular matrix antigens which are expressed during Strongylocentrotus purpuratus development. Immunofluorescent localization reveals antigen in the cortical granules of eggs and in the hyaline layer from fertilization through to gastrula. Primary (skeletogenic) mesenchyme and two secondary mesenchyme derivatives (blastocoelar cells and pigment cells) express antigen after their release into the blastocoel and maintain it throughout the remainder of larval development. The antibody allows detailed descriptions of blastocoelar cells, a prominent yet poorly described fibroblast-like mesenchyme lineage. In adults antigen is localized to the organic matrix which invests the calcified stereom of the test and spines. Immunogold electron microscopy shows antigen in the cortical granules of eggs, and on cell surfaces, within membrane bound vesicles, and within the Golgi apparatus of mesenchyme cells. On western blots a confluent smear of antigen (Mr primarily >$180K) is present in eggs, but is resolved into seven antigen bands (Mr from 35K to >$200K) after fertilization. A prominent antigen at 140K is newly expressed coincident with mesenchyme immunoreactivity. Antigens at 140K and 120K fade as prisms develop into plutei, while an antigen at 105K appears in older larvae. In adult test six antigens are shared with larvae, and there are two novel antigens at 75K and 110K. Immunoreactivity is eliminated by digestion of samples with endoglycosidase F, is reduced by periodate oxidation, but is unaffected by boiling. Calcium-magnesium-free-seawater or 1M glycine extract a subset of antigens from dissociating embryos, leaving a complementary subset of antigens associated with cell membranes. Membrane antigens are not extracted, at 4 C, with high or low ionic strength, organic solvents, or non-ionic detergents but are solubilized by ionic detergents. Whole antibody has no effect on development of embryos cultured in it from fertilization on, nor is there a specific effect from injection of antibody into the blastocoel of developing embryos. The shape of dissociated cells cultured in Sp12 whole antibody is markedly constrained compared to that of controls, however, this difference is not seen in cells incubated in Sp12 Fab fragments. Sp12 appears to recognize a carbohydrate moiety shared by ten glycoproteins of widely variable Mr and cell membrane affinity which are differentially expressed on mesenchyme throughout S. purpuratus development. The antibody does not disrupt development in vivo, but does affect cell shape in vitro, probably by crosslinking cell surface antigens. / Graduate

No description available.

No description available.

Thesis (M.S.)--California Polytechnic State University, 2009. / Mode of access: Internet. Title from PDF title page; viewed on Jan. 6, 2010. Major professor: Dean E. Wendt. "Presented to the faculty of the Biological Sciences Department, California Polytechnic State University." "In partial fulfillment of the requirements for the degree [of] Master of Science in Biological Sciences." "September 2009." Includes bibliographical references (p. 61-69).

Thesis (M.S.) in Marine Biology--University of Maine, 2009. / Includes vita. Includes bibliographical references (leaves 131-136).