In recent years, second harmonic generation (SHG) microscopy has revolutionised the field of biological imaging by offering a new means of visualising the fine structures of collagen tissues with excellent image penetration while minimising photodamage. This project involves the design and construction of a SHG microscope that is built around a compact femtosecond fibre laser for collagen imaging. Operating at 1032 nm, the microscope has demonstrated a penetration depth of beyond 320 microns in collagen, which is considerably superior to depths of 250 to 300 microns achievable with a conventional SHG microscope coupled to a Ti:sapphire excitation laser. The imaging characteristics of the microscope have been tested with a modified sample of bovine pericardium. The results indicate the microscope is polarisation-sensitive to the tissue structure and is capable to detecting signal changes at 10 μm resolution. This thesis will describe in detail, to our best knowledge, the first SHG microscope equipped with a compact and robust all-fibre femtosecond 1032 nm laser source.

Implementation of the ultrasonic second harmonic generation has typically been restricted to simple setups such as through-transmission or Rayleigh surface waves. Recent research has evaluated the second harmonic generation in P- and SV- waves reflected from a stress-free surface to enable the single-sided interrogation of a specimen. This research considers the second harmonic generation in an aluminum specimen, which is analytically evaluated using an approach based on the perturbation method. Here, the model is chosen to mimic an experimental setup where a longitudinal wave is generated at an oblique angle and the reflected wave is detected using a set of wedge transducers. Due to mode conversion at the interface of the wedge and the specimen, it is necessary to evaluate longitudinal and shear waves, determining all second harmonic waves generated in the bulk and at the stressfree boundary. The theoretically developed model is then implemented in a commercial finite element code, COMSOL, using increasing fundamental wave amplitudes for different values of third order elastic constants. The results of this computational model verify the analytical approach and the proposed measurement setup, taking into account assumptions and approximations of the solution procedure. Furthermore, the computational model is used to draw important conclusions relevant to the experimental setup, including the need to avoid evolving surface waves and interactions with diffracted waves. These numerical results are used to develop a recommendation for the measurement position and incident angle. Finally, the nonlinearity of two different aluminum specimens is measured with the suggested measurement setup and the results confirm the feasibility of the single-sided determination of the acoustic nonlinearity using reflected bulk waves.

Thesis (Ph. D.)--University of Texas at Austin, 2007. / Vita. Includes bibliographical references.

Thesis (Ph. D.)--University of Texas at Austin, 2006. / Vita. Includes bibliographical references.

Results of research on surface emission from a waveguide due to second-harmonic generation are presented. This concept has been applied and demonstrated here in the InP-InGaAsP material system for the first time, using a fundamental wavelength of 1.32 |im and a harmonic surface emission at 660 nm. The surface emission is the result of the nonlinear mixing of two counterpropagating modes in a waveguide. The theory of nonlinear optics that produces this effect is explained, leading up to a model that describes the behaviour of the surface emitting waveguide (SEWG). This model is then used to design a pseudo-optimized structure that was subsequently grown, characterized and tested. Device performance and behaviour are compared with theoretical predictions. / Thesis / Master of Engineering (ME)

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Two applications of second harmonic generation (SHG), a nonlinear optical technique, are studied. First, Fresnel factors are used with a bond model to describe SHG from vicinal silicon at five incidence angles: 7.5°, 22°, 30°, 45°, and 52°. Second, a prototype apparatus for applying SHG to enhance imaging capabilities of optical coherence tomography, a microscopy technique used in many biological fields, is briefly described. / text

I propose and demonstrate a method by which barium titanate nanoparticle clusters can be used as exogenous contrast agents in Second Harmonic Optical Coherence Tomography imaging systems to localize and highlight desired regions of tissue. SH-OCT has previously been used to identify collagen within OCT images. However, SH-OCT signals from collagen are highly susceptible to inferior reflector artifacts because most of the second harmonic generated light is forward scattered. Second harmonic generating nanoparticle clusters exhibit high scattering properties, which can give them the advantage of backscattering a large quantity of second harmonic light while attenuating the forward scattered light. In this research project, a mathematical model is proposed in which the backward to forward scattering ratio of second harmonic generated light from nanoparticle layers is exponentially proportional to the thickness of the layer. This model was supported by measurements of the backward to forward scattering ratio of second harmonic light in barium titanate nanoparticles layers. This indicates that nanoparticle clusters can be designed and manufactured with the proper thickness so that they generate a large second harmonic signal without creating inferior reflector artifacts.

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy