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by Anskar Yu-hung Leung. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1992. / Includes bibliographical references (leaves 147-163). / Chapter Section I --- Literature review / Chapter Chapter I.1. --- The epididymis - its structures and functions --- p.1 / Chapter Chapter I.2. --- Cellular mechanisms of transepithelial electrolyte transport in epididymis and other exocrine tissues --- p.5 / Chapter Chapter I.3 --- Signal transduction mechanism of chloride secretion in epididymis and other exocrine tissue --- p.11 / Chapter Chapter I.4 --- Significance of chloride secretion by the epididymal epithelium and the objectives of the study --- p.17 / Chapter Section II --- General methods / Chapter Chapter II.1. --- Tissue culture from the rat cauda epididymis --- p.20 / Chapter Chapter II.2. --- The short-circuit current technique --- p.30 / Chapter Chapter II.3. --- The immunofluorescence technique --- p.39 / Chapter Chapter II.4. --- Intracellular adenosine 3':5' cyclic monophosphate (cAMP) measurement --- p.43 / Chapter Chapter II.5 --- Intracellular Ca2+ measurement using the microfluorimetric technique --- p.49 / Chapter Section III --- Results / Chapter Chapter III.1. --- Studies on the neural and humoral controls of eletrogenic chloride secretion in rat epididymis --- p.55 / Chapter Chapter III.2. --- Local control of electrogenic chloride secretion in rat epididymis 226}0ؤThe role of the calcitonin gene-related peptide --- p.80 / Chapter Chapter III.3. --- Characterization of intracellular Ca2+ store using ATP as the calcium mobilizing agonist --- p.94 / Chapter Chapter III.4. --- Ca2+ handling mechanisms in single cultured rat epididymal cells --- p.106 / Chapter Chapter III.5. --- Studies on the effector process in the stimulus-secretion coupling- characterization of apical Cl- conductance in cultured rat cauda epididmal cells --- p.114 / Chapter Chapter III.6. --- Effects of secretory agonists on transepithelial Cl- transport and intracellular Ca2+ concentration in cultured human epididymal epithelium --- p.132 / Chapter Section IV --- General discussion --- p.142 / Chapter Section V --- References --- p.147 / Appendix --- p.164

by Zhao Wei. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 58-74). / Declaration --- p.i / Acknowledgements --- p.iii / Summary --- p.iii / Abbreviations --- p.v / Chapter CHAPTER 1 --- LITERATURE REVIEW --- p.1 / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Anatomy and functions of the epididymis --- p.1 / Chapter 1.2.1 --- Anatomy of the epididymis --- p.1 / Chapter 1.2.2 --- Histology and cytology of the epididymis --- p.2 / Chapter 1.2.3 --- Innervation and vasculation of the epididymis --- p.4 / Chapter 1.2.4 --- Sperm maturation and fertility in the epididymis --- p.6 / Chapter 1.2.5 --- Absorptive and secretory process of the epididymal epithelium --- p.8 / Chapter 1.2.6 --- Regulation of epididymal anion secretion --- p.9 / Chapter 1.3 --- Project aims --- p.12 / Chapter CHAPTER 2 --- METHODS AND MATERIALS --- p.13 / Chapter 2.1 --- Tissue preparation --- p.13 / Chapter 2.2 --- Light microscopic immunohistochemistry --- p.13 / Chapter 2.3 --- Cell culture and radioimmunoassay --- p.14 / Chapter 2.4 --- Cell culture and short -circuit current (Isc) measurement --- p.15 / Chapter 2.5 --- Reagents --- p.16 / Chapter 2.6 --- Statistical analysis --- p.16 / Chapter CHAPTER 3 --- LOCALIZATION AND DISTRIBUTION OF angiotensin ii in the rat epididymis --- p.17 / Chapter 3.1 --- Introduction --- p.17 / Chapter 3.2 --- Results --- p.18 / Chapter 3.2.1 --- Immunohistochemical localization of angiotensin II --- p.18 / Chapter 3.2.2 --- Angiotensin II activity measured by radioimmunoassay --- p.31 / Chapter 3.3 --- Discussion --- p.31 / Chapter CHAPTER 4 --- CELLULAR MECHANISM OF cAMP-DEPENDENTE BICARBONATE SECRETION IN RAT EPIDIDYMAL EPITHELIUM --- p.38 / Chapter 4.1 --- Introduction --- p.38 / Chapter 4.2 --- Results --- p.40 / Chapter 4.2.1 --- Effects of Cl and HCO3- on cyt-cAMP stimulated Isc in rat epididymal epithelia --- p.40 / Chapter 4.2.2 --- Effects of inhibitors on the cpt-cAMP stimulated Iscin HCO3- free solution --- p.41 / Chapter 4.2.3 --- Effects of inhibitors on the cpt-cAMP stimulated Isc in Cl-free solution --- p.41 / Chapter 4.2.4 --- Effect of hexokinase on cyt-cAMP induced Isc --- p.48 / Chapter 4.3 --- Discussion --- p.49 / Chapter CHAPTER 5 --- CONCLUSION --- p.55 / References --- p.58 / Publications --- p.74

Thèse : Zoologie : Faculté des sciences de Paris : 1859. / Titre provenant de l'écran-titre.

published_or_final_version / Physiology / Doctoral / Doctor of Philosophy

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Female mosquitoes lay eggs after the consuming a blood meal. The iron storage protein ferritin could be involved with iron movement among body tissues in insects. Mosquito ferritin is present in hemolymph (blood) and the messages for the heavy and light chain subunits contain secretion signal sequences. These subunits may be targeted to the secretory pathway. We found that Aedes aegypti larval cells (CCL-125) exposed to iron as ferric ammonium citrate (FAC) increased ferritin secretion in a dose-dependent manner. In order to study the secretory pathway of ferritin, we attempted to disrupt the Golgi by treating CCL-125 cells with brefeldin A (BFA) and monensin. Unexpectedly, neither BFA nor monensin inhibits iron-induced ferritin secretion. These data suggest that either CCL-125 cells are highly resistant to these agents or ferritin is secreted independently of the classical ER-Golgi pathway.

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No description available.