Abstract The aims were to examine the importance of regeneration by seeds, the influence of plant traits and disturbances, and the role of seed-seedling conflicts in regeneration and in the determination of vegetation structure. The study was carried out at in a subarctic alpine area (Kilpisjärvi 69°01'N 20°50E', Finland). Seed bank and seedling densities were high in many plant communities (ranges 99 -1109 viable seeds/m2 and 0.2-227 seedlings/m2, respectively). Effective seedling recruitment is reflected in vegetation as a high proportion of plants with poor or no vegetative reproduction ability. This development may take place in meadows and snowbeds where herbs (e.g. Gnaphalium supinum, Sibbaldia procumbens, Veronica alpina and Viola biflora) are abundant. On the other hand, the low proportion of these plants in heath vegetation reflects ineffective seedling recruitment. Floristic similarities between the consecutive phases in the regeneration pathway may be low despite effective seedling recruitment. Clonality, large and small seed sizes and appendaged diaspores limit the movement of species from phase to phase. Generally, disturbances facilitate effective regeneration by seeds. Grazing promotes species with large seed banks and is therefore one reason for high seed bank densities. Freezing and melting processes negate a negative influence of altitude on seed bank densities in the phase of seedlings. However, if disturbances are severe and continuous and the soil is compact, unstable or dry, disturbances are not beneficial. The same is true if there is a shift in the species composition of seedlings from gaps to closed vegetation. This phenomenon occurred in a rich meadow. Seed-seedling conflicts limit regeneration by seeds in low-herb snowbeds and Ranunculus glacialis-Gymnomitrion snowbeds. Vegetative reproduction and infrequent pulses of seedling recruitment negate an influence of short-term seedling recruitment on the spatial structure of vegetation. Extreme conditions, such as low temperatures, instability of the soil and late snowmelt modify the influence of factors that are important in more moderate conditions. To conclude, all transitions limit regeneration by seeds. However, favourable conditions (e.g. moist conditions in a meadow) partly eliminate the obstacles against seedling emergence. Regeneration by seeds therefore has a major impact on the dynamics and structure of vegetation. In heath vegetation, where bare soils are dry and the moss cover is thick, large seed banks and seed rains do not guarantee effective seedling recruitment. The regeneration process is reduced in the early phases, and plants that reproduce primarily by seeds have a minor role in vegetation. The accumulation of seed banks is effective in these circumstances.

Tendências atuais em restauração evidenciam a importância de propiciar o retorno das funções ecossistêmicas e ecológicas. Porém muitas áreas possuem baixo potencial para reestabelecimento de processos ecológicos devido à perda da resiliência, o que faz da restauração ecológica um processo desafiador. Para o sucesso das atividades propostas, é essencial que no decorrer do projeto os sistemas sejam capazes de retomar sua trajetória sucessional e dinâmica temporal, sem necessidade de futuras intervenções humanas. O objetivo desta tese foi avaliar os processos iniciais de restauração ecológica em áreas degradadas por mineração de carvão, através da análise de trajetórias sucessionais envolvendo: (1) taxas de crescimento e mortalidade de mudas nativas introduzidas e sua relação com atributos funcionais de plantas; (2) chuva de sementes associada, ou não, ao uso de poleiros artificiais e sua relação com regenerantes de espécies lenhosas; e (3) regeneração natural e sua relação com fatores abióticos e bióticos. Mudas de espécies nativas introduzidas sob condições limitantes (devido ao alto nível de impacto) apresentaram diferentes performances e relações com atributos, por vezes distintas do esperado para espécies sob condições naturais. A limitação de sementes nas áreas em restauração pode ser, potencialmente, reduzida pelo uso de poleiros artificiais, aumentando especialmente sementes de espécies não-pioneiras e zoocóricas. No entanto, não foi verificada relação entre a chuva de sementes e a taxa de recrutamento. Fatores abióticos, relacionados à composição química do solo, e fatores bióticos, como a presença de gramíneas exóticas, foram as variáveis com maior influência negativa sobre a regeneração natural. Este estudo evidencia a possibilidade do retornar do processo de sucessão natural em áreas de mata ciliar que foram profundamente alteradas por mineração de carvão, em um tempo relativamente curto. Entretanto, recomendamos o uso de objetivos realísticos e intermediários, bem como monitoramentos detalhados nas fases iniciais. / In current projects of ecological restoration, the return of ecosystem functions and natural processes is an important aim. However, many areas have lost their resilience due the high damage they have been submitted to, and this makes the ecological restoration a challenging process. For the success of the proposed activities, it is essential that during the project, the ecosystem can return to its successional trajectory without necessity of strong human intervention in the future. The goal of this thesis was to evaluate the initial processes of ecological restoration in areas severally degraded by coal mining, through analyses of successional trajectories regarding: (1) growth and mortality rates of planted samplings and their relationship to functional traits; (2) seed rain, associated or not, to perches and its relationship to natural regeneration of woody species; and (3) natural regeneration of woody species and its relationship to abiotic and biotic variables. Planted saplings under limited conditions (due the high impact of mining) showed to have distinct performance and trait relationships, somewhat different to the expected for species under natural conditions. The study indicates that the limitation of seeds in areas under ecological restoration can be reduced by the use of perches, increasing especially the number of seeds of non-pioneer and zoocoric species. Nevertheless, we did not found a relation between seed rain and recruitment rate. Soil chemistry (abiotic variable) and the cover of exotic grasses (biotic variable) were the variables with the strongest negative impact on natural regeneration. We conclude that it is possible to return to successional processes in areas of riparian forest severely damaged by coal mining activities in a relatively short time, but recommend the use of realistic, intermediate restoration goals and detailed monitoring in early restoration phases.

The objectives of this research were to investigate the ecological importance of soil seed reserves and seed rain on regeneration of a good condition sagebrush- grass range vegetation after a wildfire and draw conclusions leading to better understanding and management of such ecosystems. Investigations were conducted for two successive years on a community where major plants were neither rhizomatous nor sprouting. In such cases soil seed reserves and seed rain have to be the main source of regeneration. In addition to monitoring soil seed reserves and seed rain, vegetation changes during the past two years and the historical conditions of the study area were examined. Study of germinable soil seed reserve dynamics showed that fire can have a destructive effect on this portion of the community. Cheatgrass soil seed reserves were high even in good condition sagebrush-grass vegetation. Although fire reduced the Bromus tectorum seed bank by half, the cover of this grass increased to almost twice the level observed on the control (unburned) plots a year later. This shows the enormous reproductive capacity of this highly competitive weed species following a wildfire. Even though the pre-burn vegetation contained a high proportion of native perennial plants, soil seed reserves and seed rain had very small proportions of their germinable seeds. Timing of the fire is likely important in controlling undesirable range plants and their seeds. Had the fire occurred earlier when more seeds were attached to the culms, greater reduction in cheatgrass probably would have been obtained. Timing of the fire was just right to control sagebrush, because it occurred before their seed set and complete destruction of this species was achieved. Mormon tea was the only shrub to reestablish its cover relatively rapidly. This was related to its strong ability to sprout from root crowns. Greater germinable soil seed reserves were found under shrub canopies than in the inter spaces. This is probably related to the semi-logarithmic dispersal of seed where seed fall is greatest closest to mother plants (Harper 1977). Since flammable fuel follows the same pattern, it was found that fire has a serious impact on soil seed reserves at "hot" points, but temperatures were apparently not hot enough to cause much damage on seed banks at "cold" points in the former interspaces. Since soil seed reserves accumulate in significantly higher proportions in the surface 0-2 cm, fire has a more serious impact on the seeds in surface soil than those lower lower down. Variance of the germinable seed rain was so high that none of the grand totals, life forms totals and species values were statistically significant at alpha

Tendências atuais em restauração evidenciam a importância de propiciar o retorno das funções ecossistêmicas e ecológicas. Porém muitas áreas possuem baixo potencial para reestabelecimento de processos ecológicos devido à perda da resiliência, o que faz da restauração ecológica um processo desafiador. Para o sucesso das atividades propostas, é essencial que no decorrer do projeto os sistemas sejam capazes de retomar sua trajetória sucessional e dinâmica temporal, sem necessidade de futuras intervenções humanas. O objetivo desta tese foi avaliar os processos iniciais de restauração ecológica em áreas degradadas por mineração de carvão, através da análise de trajetórias sucessionais envolvendo: (1) taxas de crescimento e mortalidade de mudas nativas introduzidas e sua relação com atributos funcionais de plantas; (2) chuva de sementes associada, ou não, ao uso de poleiros artificiais e sua relação com regenerantes de espécies lenhosas; e (3) regeneração natural e sua relação com fatores abióticos e bióticos. Mudas de espécies nativas introduzidas sob condições limitantes (devido ao alto nível de impacto) apresentaram diferentes performances e relações com atributos, por vezes distintas do esperado para espécies sob condições naturais. A limitação de sementes nas áreas em restauração pode ser, potencialmente, reduzida pelo uso de poleiros artificiais, aumentando especialmente sementes de espécies não-pioneiras e zoocóricas. No entanto, não foi verificada relação entre a chuva de sementes e a taxa de recrutamento. Fatores abióticos, relacionados à composição química do solo, e fatores bióticos, como a presença de gramíneas exóticas, foram as variáveis com maior influência negativa sobre a regeneração natural. Este estudo evidencia a possibilidade do retornar do processo de sucessão natural em áreas de mata ciliar que foram profundamente alteradas por mineração de carvão, em um tempo relativamente curto. Entretanto, recomendamos o uso de objetivos realísticos e intermediários, bem como monitoramentos detalhados nas fases iniciais. / In current projects of ecological restoration, the return of ecosystem functions and natural processes is an important aim. However, many areas have lost their resilience due the high damage they have been submitted to, and this makes the ecological restoration a challenging process. For the success of the proposed activities, it is essential that during the project, the ecosystem can return to its successional trajectory without necessity of strong human intervention in the future. The goal of this thesis was to evaluate the initial processes of ecological restoration in areas severally degraded by coal mining, through analyses of successional trajectories regarding: (1) growth and mortality rates of planted samplings and their relationship to functional traits; (2) seed rain, associated or not, to perches and its relationship to natural regeneration of woody species; and (3) natural regeneration of woody species and its relationship to abiotic and biotic variables. Planted saplings under limited conditions (due the high impact of mining) showed to have distinct performance and trait relationships, somewhat different to the expected for species under natural conditions. The study indicates that the limitation of seeds in areas under ecological restoration can be reduced by the use of perches, increasing especially the number of seeds of non-pioneer and zoocoric species. Nevertheless, we did not found a relation between seed rain and recruitment rate. Soil chemistry (abiotic variable) and the cover of exotic grasses (biotic variable) were the variables with the strongest negative impact on natural regeneration. We conclude that it is possible to return to successional processes in areas of riparian forest severely damaged by coal mining activities in a relatively short time, but recommend the use of realistic, intermediate restoration goals and detailed monitoring in early restoration phases.

Resumo: Em florestas tropicais, ainda são incipientes os estudos que tratam da influência dos bambus na dinâmica da vegetação. Pela grande capacidade de se expandir rapidamente no ambiente seja por crescimento vegetativo ou por produção massiva de sementes, os bambus promovem alterações significativas na estrutura das comunidades vegetais. Desta forma, esse estudo teve como objetivo avaliar o ciclo de vida das plantas em ambientes com (B) e sem bambus (SB) em uma área de floresta Atlântica densamente ocupada pelo bambu nativo Guadua tagoara. Os objetivos específicos deste estudo foram separados em capítulos. Para o capítulo 1, conhecer a composição de aves associadas aos ambientes com e sem bambus, identificar as aves que compõem a guilda de dispersores de sementes de Euterpe edulis, Sloanea guianensis e Virola bicuhyba e avaliar o potencial de dispersão das aves registradas em censos. Para o capítulo 2, avaliar o padrão espacial dos estágios iniciais da regeneração da comunidade de plantas nos ambientes B e SB. Finalmente para o capítulo 3, quantificar as perdas de propágulos e as probabilidades de transição entre cada etapa demográfica das três espécies de plantas selecionadas, e identificar os gargalos demográficos do recrutamento que podem colapsar a regeneração natural das três espécies de plantas nos ambientes B e SB. Verificamos que nos ambientes B foi registrado maior número de espécies de aves do que em ambientes SB. A maioria das aves registradas em censos nos dois ambientes foi insetívora, seguida por espécies frugívoras. A efetividade da dispersão de Euterpe, Sloanea e Virola, esteve restrita a um pequeno grupo de dispersores efetivos. As aves que mais contribuíram para a dispersão de Virola ocorreram mais freqüentemente em B. Este padrão foi similar para Sloanea, enquanto Euterpe apresentou um padrão misto, com algumas espécies de aves... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Studies about the bamboo influence in the plant dynamic process are still incipient. Given that bamboos are able to quickly expand in environment by either vegetative growth or massive seed production, bamboos promote significant changes in plant community structure. Thus, this work aimed at assessing the plant life cycles in bamboo (B) and non bamboo stands (NB) in an Atlantic forest area where the native bamboo Guadua tagoara occurs at high densities. The specific goals of this study were showed in chapters. For chapter 1, to know bird species associated with bamboo and non bamboo stands, identify the birds which belong to seed dispersers guild of Euterpe edulis, Sloanea guianensis and Virola bicuhyba and evaluate dispersal effectiveness and potential contribution to seed dispersal for the three plant species. For chapter 2, assess the spatial pattern of early regeneration stages of plant communities in B and NB stands. Finally, for chapter 3, quantify propagule losses as well as the probabilities of transition between each stage for all three plant species, and identify the demographic bottlenecks in recruitment that could collapse natural regeneration of the three plant species in B and NB stands. We verified that a higher number of bird species was registered in B stands than in NB. Most of registered birds in both B and NB stands were insectivorous followed by frugivorous species. The dispersal effectiveness of Euterpe, Sloanea and Virola depended on a restricted subset of effective bird dispersers. Birds which mostly contributed to the seed dispersal of Virola occurred more frequently in B. This pattern was similar for Sloanea while Euterpe exhibited a mixed pattern with some bird species contributing to the dispersal in the B stands and other species contributing in NB stands. We conclude that a substantial number of frugivorous bird species can favor borders of bamboo patches... (Complete abstract click electronic access below) / Orientador: Marco Aurélio Pizo Ferreira / Coorientador: Pedro Diego Jordano Barbudo / Banca: Luciana Spinelli Araujo / Banca: Mauro Galetti Rodrigues / Banca: Leonor Patrícia Cerdeira Morellato / Banca: Eliana Cazetta / Doutor

Tendências atuais em restauração evidenciam a importância de propiciar o retorno das funções ecossistêmicas e ecológicas. Porém muitas áreas possuem baixo potencial para reestabelecimento de processos ecológicos devido à perda da resiliência, o que faz da restauração ecológica um processo desafiador. Para o sucesso das atividades propostas, é essencial que no decorrer do projeto os sistemas sejam capazes de retomar sua trajetória sucessional e dinâmica temporal, sem necessidade de futuras intervenções humanas. O objetivo desta tese foi avaliar os processos iniciais de restauração ecológica em áreas degradadas por mineração de carvão, através da análise de trajetórias sucessionais envolvendo: (1) taxas de crescimento e mortalidade de mudas nativas introduzidas e sua relação com atributos funcionais de plantas; (2) chuva de sementes associada, ou não, ao uso de poleiros artificiais e sua relação com regenerantes de espécies lenhosas; e (3) regeneração natural e sua relação com fatores abióticos e bióticos. Mudas de espécies nativas introduzidas sob condições limitantes (devido ao alto nível de impacto) apresentaram diferentes performances e relações com atributos, por vezes distintas do esperado para espécies sob condições naturais. A limitação de sementes nas áreas em restauração pode ser, potencialmente, reduzida pelo uso de poleiros artificiais, aumentando especialmente sementes de espécies não-pioneiras e zoocóricas. No entanto, não foi verificada relação entre a chuva de sementes e a taxa de recrutamento. Fatores abióticos, relacionados à composição química do solo, e fatores bióticos, como a presença de gramíneas exóticas, foram as variáveis com maior influência negativa sobre a regeneração natural. Este estudo evidencia a possibilidade do retornar do processo de sucessão natural em áreas de mata ciliar que foram profundamente alteradas por mineração de carvão, em um tempo relativamente curto. Entretanto, recomendamos o uso de objetivos realísticos e intermediários, bem como monitoramentos detalhados nas fases iniciais. / In current projects of ecological restoration, the return of ecosystem functions and natural processes is an important aim. However, many areas have lost their resilience due the high damage they have been submitted to, and this makes the ecological restoration a challenging process. For the success of the proposed activities, it is essential that during the project, the ecosystem can return to its successional trajectory without necessity of strong human intervention in the future. The goal of this thesis was to evaluate the initial processes of ecological restoration in areas severally degraded by coal mining, through analyses of successional trajectories regarding: (1) growth and mortality rates of planted samplings and their relationship to functional traits; (2) seed rain, associated or not, to perches and its relationship to natural regeneration of woody species; and (3) natural regeneration of woody species and its relationship to abiotic and biotic variables. Planted saplings under limited conditions (due the high impact of mining) showed to have distinct performance and trait relationships, somewhat different to the expected for species under natural conditions. The study indicates that the limitation of seeds in areas under ecological restoration can be reduced by the use of perches, increasing especially the number of seeds of non-pioneer and zoocoric species. Nevertheless, we did not found a relation between seed rain and recruitment rate. Soil chemistry (abiotic variable) and the cover of exotic grasses (biotic variable) were the variables with the strongest negative impact on natural regeneration. We conclude that it is possible to return to successional processes in areas of riparian forest severely damaged by coal mining activities in a relatively short time, but recommend the use of realistic, intermediate restoration goals and detailed monitoring in early restoration phases.

Orientador: Wesley Rodrigues Silva / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-26T19:16:14Z (GMT). No. of bitstreams: 1 Zaniratto_CristianePatricia_M.pdf: 6906690 bytes, checksum: 61183ae4c09cd06d8858d6ee6aa85be7 (MD5) Previous issue date: 2015 / Resumo: A degradação em florestas tropicais afeta as relações mutualísticas entre plantas e frugívoros, o que é refletido na chuva de sementes. Devido ao comportamento alimentar e à alta capacidade de deslocamento, aves e morcegos frugívoros são importantes agentes no processo de restauração, pois transportam propágulos de fontes bem preservadas para áreas impactadas. Este trabalho avaliou a chuva de sementes zoocóricas em um mosaico fisionômico de restauração de Mata Atlântica no estado de São Paulo, considerando a contribuição de aves e morcegos, bem como a influência de fatores ambientais sobre a chuva de sementes. As sementes foram analisadas quanto ao sistema de dispersão (ornitocóricas e quiropterocóricas), grupo sucessional (pioneiras e não pioneiras) e origem (espécies do plantio e colonizadoras). Também foi comparada a chuva de sementes entre áreas com diferentes fisionomias. A estação chuvosa apresentou maior abundância e riqueza de propágulos, indicando uma maior disponibilidade de recursos para a fauna durante esse período. As variáveis ambientais não explicaram a abundância total de sementes, a abundância de sementes quiropterocóricas e a abundância de sementes pioneiras. Esses resultados foram influenciados por algumas espécies amplamente coletadas em todos os pontos de amostragem, o que dificultou a formação de um padrão. A abundância de sementes ornitocóricas foi explicada por uma relação positiva com a densidade de plantas zoocóricas, sugerindo que essas espécies constitui um fator de atração importante para a avifauna. A abundância de sementes não pioneiras foi relacionada negativamente à abertura de dossel e positivamente à densidade de sub-bosque. A riqueza total e a riqueza de sementes ornitocóricas relacionaram-se negativamente à abertura de dossel. As riquezas de sementes quiropterocóricas, pioneiras e não pioneiras foram relacionadas negativamente à abertura de dossel e positivamente à densidade de sub-bosque. As fisionomias em estágio avançado de regeneração apresentaram maior riqueza na chuva de sementes, embora a abundância não tenha diferido entre as áreas. Tais resultados sugerem que a complexidade estrutural do ambiente um é um fator importante para a chuva de sementes, atraindo mais dispersores. Registrou-se maior abundância e riqueza de espécies ornitocóricas, o que mostra que a avifauna contribui mais para a recuperação da área. As espécies pioneiras também foram mais abundantes e mais ricas. A baixa dispersão de sementes não pioneiras é preocupante, pois indica que dispersores de médio e grande porte não frequentam a maior parte da área de estudo, atrasando o processo de regeneração natural. As espécies colonizadoras apresentaram maior riqueza, embora não tenha havido diferença entre a abundância de espécies colonizadoras e do plantio. Esse resultado mostra que, ao longo dos anos, houve um fluxo de sementes de áreas fontes para as áreas em processo de regeneração. A análise da chuva de sementes na área de estudo permite concluir que a regeneração está ocorrendo espontaneamente, pois o transporte de diásporos existe em todo o mosaico de restauração, entretanto, a maior riqueza e abundância de espécies pioneiras mostram a necessidade de ações de manejo para acelerar a colonização por frugívoros de médio e grande porte, incrementando a chuva de sementes local / Abstract: The degradation of tropical forests affects the mutualistic interaction between plants and frugivores, with reflexes in seed rain. Due to their feeding behavior and high capacity of displacement, birds and bats are important agents in restoration process, because they carry the seeds from well-preserved sources to degraded ones. This work evaluated the zoochorous seed rain in a restoration physiognomic mosaic of Atlantic Forest in São Paulo state, considering the birds and bats contribution, as well the influence of environmental factors on the seed rain. Seeds were analyzed according to their dispersal system (dispersed by birds or bats), successional group (pioneer and non-pioneer) and origin (planted and colonizing species). The seed rain between physiognomies is also compared. The abundance and richness of seeds were higher in the rainy season, which points to a high resource availability to fauna in this period. The environmental variables did not explain the overall abundance, the abundance of chiropterochorous seeds and the abundance of pioneer seeds. These results were influenced by some species largely collected in all sampling points, which made difficult a pattern. The abundance of ornitochorous seeds was explained by density of zoochorous plants, suggesting that these species are an important attraction factor for birds. The abundance of non-pioneer seeds was negatively related to canopy opening and positively related to understory density. The overall richness and the richness of ornitochorous seeds were negatively related to canopy opening. The richness of chiropterochorous, pioneer and non-pioneer seeds were negatively related to canopy opening and positively related to understory density. The physiognomies under advanced regeneration stages had higher seed richness, although the abundance did not differ between areas. These results show that the structural complexity of environment is an important factor to seed rain, by attracting more dispersers. There was a higher abundance and richness of ornitochorous seeds, which shows that birds contributed more to forest recovery. Pioneer species are also more abundant and richer. The low dispersion of non-pioneer seeds is worrying, once suggests that medium and big size dispersers don¿t occur in the most of the study area, delaying the natural restoration process. Colonizing species were richer, although the abundance between colonizing and planted seeds species did not differ. It shows that there was a seed flow from source areas to restored ones over the years. The seed rain analysis allows the conclusion that restoration is occurring spontaneously in the studied area, since transportation of seeds are occurring across all the restoration mosaic. However, the high richness and abundance of pioneer species points to the necessity of management actions in order to accelerate the colonization by medium and large size frugivorous, improving the local seed rain / Mestrado / Ecologia / Mestra em Ecologia

Big sagebrush (Artemisia tridentata Nutt.) is the most widespread and common shrub in the sagebrush biome of western North America. Of the three most common subspecies of big sagebrush (Artemisia tridentata), mountain big sagebrush (ssp. vaseyana; MBS) is the most resilient to disturbance, but still requires favorable climactic conditions and a viable post-fire seedbank for successful unassisted recovery. This study was designed to assess MBS seed production throughout post-fire recovery. We performed 2 pilot studies to develop methods for estimating seed production and plant age. The results of the pilot studies and a space-for-time substitution strategy were used to measure seed production on 13 sites ranging from 10-33 years post-fire. We hypothesized that seed rain (mean seeds produced/ m2) would peak before stand density had maximized due to decreasing individual plant fecundity (mean seeds produced/ plant) in high density stands. We measured population density and individual plant fecundity for three size classes of MBS and used forward stepwise regression analysis to identify environmental factors influencing seed production over time. Density for small (basal stem diameter (BSD) <1 cm) and medium-sized (BSD=1-3 cm) plants was consistently low and was not affected by time since fire (TSF), while large-sized (BSD > 3 cm) plant density increased steadily with TSF (p=0.0002). Plant fecundity decreased with TSF for all three size classes (p range = 0.019 – 0.0506), with large plants dominating reproductive output. Small and medium-sized plant fecundity was negatively correlated with winter precipitation (p range = 0.0106-0.0174), while large plant fecundity was positively correlated with winter precipitation (p<0.0001) and negatively correlated with elevation (p=0.0001). Despite losses in plant fecundity over time for all size classes, steady recruitment in population density resulted in increased seed rain (p=0.0039), suggesting that increases in stand density compensated for losses in individual plant fecundity. Results partially support our hypothesis that the time required for MBS seed rain to be maximized was not tightly bound to indicators of stand maturation. Understanding the factors that influence post-fire seed production can help land managers better manage for successful recovery by providing them with tools for evaluating seed production capabilities of MBS communities.

Thesis (MScConEcol)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Acacia saligna, or Port Jackson, is an Australian Acacia which has spread throughout the Western and Eastern Cape provinces of South Africa where it has become invasive and a prominent feature of the vegetation. In areas where A. saligna establishes it replaces natural vegetation, alters ecosystem processes and interferes with agricultural practices. Consequently large management efforts have been made to eradicate this invasive alien tree. However, its large and persistent soil stored seed bank, even in the presence of management and biological control agents, poses a serious obstacle to its successful removal. Furthermore the reproductive output and the size of the seed bank of A. saligna over its whole distribution as well as the variation of seed production and the seed bank with environmental conditions in time and space is poorly known. Understanding the seed bank dynamics of A. saligna in time and space is essential for reducing invasive success and achieving management objectives. This study has filled this knowledge gap through studying the seed production and seed bank of A. saligna over its invaded range in South Africa, including how environmental factors influence these factors in time and space. The seed rain of A. saligna was assessed at 10 sites across its distribution in South Africa. The seed rain of A. saligna at the sites was determined through the use of seed rain traps. Twenty five traps were placed out at every site during November 2010 (pre-dehiscence) which was collected again during April 2011 (postdehiscence). The seed bank of A. saligna was estimated through sampling at 25 sites across its distribution range in South Africa. The seed bank was sampled during April 2010 (postdehiscence), November 2010 (pre-dehiscence) and April 2011 (post-dehiscence) through taking 50 litter and soil samples at every site which gave a total sampling size of 3 750 for both the seed in the soil and litter over its distribution in South Africa. In addition the average tree diameter, tree density, average number of Uromycladium tepperianum induced galls per tree, the summer aridity index, De Martonne aridity index, winter concentration of precipitation, temperature of coldest month and the soil texture for every site was determined. The damage done by the seed feeding weevil, Melanterius compactus, was also estimated for the seed rain study sites. Water availability during the hot summer months was assessed as the most important factor governing seed production and seed bank size. Riparian and non-riparian water regimes were shown to be important in understanding the seed bank dynamics of A. saligna over its distribution range in the Cape Floristic Region. In non-riparian A. saligna populations the seed production and consequently the size of the seed bank and its rate of accumulation is limited by both water and temperature and in riparian A. saligna populations, only by temperature. Therefore, two environmental gradients influence the seed bank dynamics of Port Jackson in South Africa. In non-riparian A. saligna populations the number of seed produced and the accumulation of seed in the seed bank generally increases along the west coast of South Africa from Clanwilliam towards Cape Town and along the south coast from Cape Town towards Port Elizabeth. Seed banks are larger closer to the coast, when A. saligna populations of similar age are compared. In riparian A. saligna populations, the number of seed produced generally increases from Port Elizabeth towards Cape Town and from Cape Town towards Clanwilliam, again, with larger seed banks being accumulated closer to the coast, when populations of similar age are compared. This study provides managers with a useful tool for prioritising management efforts. / AFRIKAANSE OPSOMMING: Acacia saligna of Port Jackson is ‘n indringerplant in Suid-Afrika wat afkomstig is uit Suid-Wes Australië. Acacia saligna is ‘n onmisbare kenmerk van die plantegroei in die Wes en Oos-Kaap. Waar Port Jackson vestig, verplaas dit natuurlike plantegroei, verander dit ekosisteem prossesse en verhinder dit landbou praktyke. Gevolglik is groot bestuurspogings aangewend om hierdie indringer plant uit te roei. In die teenwoordigheid van bestuursprogramme en biologiese beheer bly A. saligna lewensvatbaar as ‘n gevolg van die plant se groot blywende grond saadreserwes. Verder is die saad produksie en saadbank grootte van A. saligna oor sy verspreiding bereike asook hoe dit met omgewingstoestande in tyd en ruimte interreageer en varieer grootendeels onbekend. ‘n Goeie en omvattende begrip van die saadbankdinamika is belangrik om die indringings vermoë van die plant te verlaag en om bestuursdoelwitte te bereik. Hierdie studie vul die bestaande gaping in die kennis aangaande die saadbank dinamika van A. saligna aan deur die saadproduksie en die saadreserves van die indringerplant oor sy verspreiding in Suid-Afrika te bestudeer, insluitend hoe omgewingstoestande die saadbankdinamika beïnvloed in tyd en ruimte. Die saadreën vir 10 A. saligna populasies was bepaal deur die gebruik van saadlokvalle. Vyf-entwintig lokvalle was uitgeplaas in elke bestudeerde Port Jackson perseel gedurende November 2010 (voor-saadval) wat weer gaan haal is gedurende April 2011 (na-saadval). Die saadbank van A. saligna was bepaal in 25 populasies van die boom gedurende April 2010 (na-saadval), November 2010 (voor-saadval) en April 2011 (na-saadval) deur die neem van 50 blaar- en grondmonsters by elke perseel wat ‘n totaal van 3 750 blaar- en grondmonster gee oor die hele verspreiding van A. saligna in Suid-Afrika. Verder is die gemiddelde boomdeursnee, boomdigtheid, gemiddelde Uromycladium tepperianum geinduseerde galle per boom, die somers droogtheids indeks, De Martonnes droogtheids indeks, die winter konsentrasie van presipitasie, die temperatuur van die koudste maand en die grond tekstuur van elke Port Jackson stand bepaal. Die skade wat die saad voedende kewer, Melanterius compactus, aanrig aan die sade van A. saligna is ook vir die persele waar die saadreën eksperiment uitgevoer is, bepaal. Die beskikbaarheid van water vir A. saligna in die droë somermaande is bepaal as die belangrikste faktor wat die grootte van die saadproduksie en saadbank beïnvloed. Oewer en nie-oewer water omgewings is bevind as noodsaaklik om die saadbank dinamika van A. saligna oor die indringerplant se verspreidingareas in die Kaap Floristiese Streek te verstaan. In nie-oewer A. saligna populasies word saadproduksie en gevolglik die grootte en tempo van akkumulasie van die saadbank deur beide water en temperatuur omstandighede beperk, terwyl in oewer A. saligna populasies word die indringerplant slegs deur temperatuur omstandighede beperk. Gevolglik blyk dit dat twee water beskikbaarheids gradiënte bestaan waarop die plant reageer wat dan uitgedruk word in die plant se saad produksie en gevolglik ook die plant se saadbank. In nie-oewer Port Jackson populasies neem die saadproduksie en die tempo waarteen die saadbank akkumuleer algemeen toe langs die weskus van Clanwilliam na Kaapstad en van Kaapstad na Port Elizabeth, met grootter saadproduksie en saadbank akkumulasie nader aan die kusgebiede, wanneer A. saligna populasies van dieselfde ouderdom vergelyk word. In oewer Port Jackson populasies neem die saadproduksie en die tempo waarteen die saadbank akkumuleer algemeen toe van Port Elizabeth na Kaapstad en van Kaapstad na Clanwilliam, met grootter saadproduksie en saadbank akkumulasie nader aan die kusgebiede, wanneer A. saligna populasies van dieselfde ouderdom vergelyk word. Hierdie studie verskaf bestuursplanne met ‘n nuttige raamwerk waarvolgens uitroeiing en beheer programme vir A. saligna beplan kan word.

O nível de perturbação a que um ambiente está sujeito pode afetar profundamente o processo de regeneração natural, através de modificações nas fontes de regeneração. Os principais mecanismos de regeneração natural das florestas são a chuva de sementes (dispersão), o banco de sementes do solo e o estabelecimento de plântulas. O presente estudo tem como objetivo analisar uma das principais etapas da regeneração, a chuva de sementes, em áreas de perturbações naturais (clareiras) e antrópicas (área queimada) e áreas sem indícios de perturbação recente na Mata Atlântica do sul da Bahia. Para a amostragem, foram instalados coletores de sementes em ambientes de sub-bosque, de clareiras naturais e de uma área perturbada por fogo, num total de 144 coletores de 0,50 x 0,50 m, amostrados mensalmente. A chuva de sementes da Floresta Ombrófila Densa analisada foi contínua ao longo dos dois anos amostrados, mas apresentou flutuações entre períodos do ano e variações entre os ambientes analisados. Foram amostradas 41.910 sementes, pertencentes a 198 morfoespécies, sendo que as 120 que puderam ser identificadas foram distribuídas em 31 famílias e 57 gêneros. As 78 espécies indeterminadas representam apenas 256 sementes ou 0,6% do total de sementes amostradas. A menor quantidade total de sementes nesses dois anos foi observada no ambiente de clareiras (7.555, representando 1,25 sementes/m²/dia), enquanto o maior número de sementes foi observado na área queimada (13.656, ou seja, 2,17 sementes/m²/dia). Não foram constatadas diferenças significativas entre a proporção de espécies anemocóricas para os ambientes de clareiras e sub-bosque em nenhum dos anos analisados. Os ambientes mais similares foram o sub-bosque próximo à área queimada (MP) e o subbosque adjacente às clareiras (SB), seguido pelas clareiras (C) e pelo sub-bosque próximo à área queimada (MP). Os períodos de maior taxa de deposição de sementes estiveram concentrados principalmente no meio de ano para praticamente todos os ambientes. A chuva de sementes foi dominada por um pequeno número de espécies produzindo cerca de 98% do total de sementes amostradas, e com muitas espécies sendo representadas por poucas sementes ao longo do período de estudo. Essa dominância foi representada principalmente por três espécies arbóreas pioneiras nativas: Miconia mirabilis, Cecropia pachystachya e Henriettea succosa. Em análise mais detalhada da chuva de sementes destas espécies foi observado que as espécies Miconia mirabilis e Cecropia pachystachya mostraram um padrão anual de deposição de suas sementes, sugerindo que estas espécies possuem uma fenologia definida com longos períodos de deposição de sementes durante o ano. Henriettea succosa foi a espécie que obteve uma distribuição mais homogênea entre os quatros ambientes observados, porém sua maior deposição de sementes ocorreu exclusivamente no mês de abril de 1999. / The disturbance level to which a forest is exposed to may affect its dynamics, modifying the recruits sources. The main mechanisms of forest natural regeneration is seed rain (dispersion), soil seed bank and seedlings establishment. This study aims to analyze seed rain within fire and natural (gaps) disturbed areas and sites with no sign of recent disturb at Atlantic Forests of South Bahia. Sampling was taken monthly through seed rain collectors located under gaps, understorey and fire disturbed areas, in a total of 144 0,5x0,5 m collectors. Although seed rain sampling was continuous, fluctuations were noticed for distinct seasonal periods and environments. A total of 41.910 seeds were sampled, representing 198 morfo-species; 120 were identified and classified into 31 botanical families and 57 genera. The remaining 78 morfo-species were represented by 256 seeds or 0,6% of total sampled seeds. The lower quantities of seeds were observed within gaps environments (7.555 seeds, representing 1,25 seeds/m²/day), while the higher amounts were observed for the fire disturbed areas (13.656 seeds or 2,17 seeds/m²/day). No significant differences were found regarding anemochoric species occurrence between gaps and understorey environments. Similarities were found between fire disturbed areas (MP) and their surrounding understorey environments (SB) and also between gaps (C) and understorey close to fire disturbed areas (SB). The periods of seeds deposition higher rate had been mainly concentrated in the middle of the year for practically all environments. Seed rain was characterized by few species that produced around 98% of the total amount of sampled seeds, with most of the species being represented by few seeds during this study. The dominant species were represented by three native pioneer tree species: Miconia mirabilis, Cecropia pachystachya and Henriettea succosa. A more detailed analysis of the seed rain revealed that Miconia mirabilis and Cecropia pachystachya have an annual deposition pattern, suggesting that these species have a defined phenology, with long periods of seeds deposition along a year. Henriettea succosa presented the most homogeneous distribution among the observed environments, with higher levels of deposition during April 1999. Further studies are needed to understand the observed patterns of this study, especially on M. mirabilis and C. pachystachya phenology.