M. Tech. (Department of Information & Communication Technology, Faculty of Applied and Computer Sciences), Vaal University of Technology. / Many studies have evaluated and compared the existing open-sources Semantic Web platforms for ontologies development. However, none of these studies have included the dot NET-based semantic web platforms in the empirical investigations. This study conducted a comparative analysis of open-source and dot NET-based semantic web platforms for ontologies development. Two popular dot NET-based semantic web platforms, namely, SemWeb.NET and dotNetRDF were analyzed and compared against open-source environments including Jena Application Programming Interface (API), Protégé and RDF4J also known as Sesame Software Development Kit (SDK). Various metrics such as storage mode, query support, consistency checking, interoperability with other tools, and many more were used to compare two categories of platforms. Five ontologies of different sizes are used in the experiments. The experimental results showed that the open-source platforms provide more facilities for creating, storing and processing ontologies compared to the dot NET-based tools. Furthermore, the experiments revealed that Protégé and RDF4J open-source and dotNetRDF platforms provide both graphical user interface (GUI) and command line interface for ontologies processing, whereas, Jena open-source and SemWeb.NET are command line platforms. Moreover, the results showed that the open-source platforms are capable of processing multiple ontologies’ files formats including Resource Description Framework (RDF) and Ontology Web Language (OWL) formats, whereas, the dot NET-based tools only process RDF ontologies. Finally, the experiment results indicate that the dot NET-based platforms have limited memory size as they failed to load and query large ontologies compared to open-source environments.