To investigate the diversities and abundances of nitrifiers and to apply the highthroughput sequencing technologies to analyze the overall microbial community structures and functions in the wastewater treatment bioreactors were the major objectives of this study. Specifically, this study was conducted: (1) to investigate the diversities and abundances of AOA, AOB and NOB in bioreactors, (2) to explore the bacterial communities in bioreactors using 454 pyrosequencing, and (3) to analyze the metagenomes of activated sludge using Illumina sequencing. A lab-scale nitrification bioreactor was operated for 342 days under low DO (0.15~0.5 mg/L) and high nitrogen loading (0.26~0.52 kg-N/(m3d)). T-RFLP and cloning analysis showed there were only one dominant AOA, AOB and NOB species in the bioreactor, respectively. The amoA gene of the dominant AOA had a similarity of 89.3% with the isolated AOA species Nitrosopumilus maritimus SCM1. The AOB species detected in the bioreactor belonged to Nitrosomonas genus. The abundance of AOB was more than 40 times larger than that of AOA. The percentage of NOB in total bacteria increased from not detectable to 30% when DO changed from 0.15 to 0.5 mg/L. Compared with traditional methods, pyrosequencing analysis of the bacteria in this bioreactor provided unprecedented information. 494 bacterial OTUs was obtained at 3% distance cutoff. Furthermore, 454 pyrosequencing was applied to investigate the bacterial communities of activated sludge samples from 14 WWTPs of Asia (mainland China, Hong Kong, and Singapore) and North America (Canada and the United States). The results revealed huge amounts of OTUs in activated sludge, i.e. 1183~3567 OTUs in one sludge sample at 3% distance cutoff. Clear geographical differences among these samples were observed. The AOB amoA genes in different WWTPs were found quite diverse while the 16S rRNA genes were relatively conserved. To explore microbial community structures and functions in the abovementioned labscale bioreactor and a full-scale bioreactor, over six gigabases of metagenomic sequence data and 150,000 paired-end reads of PCR amplicons were generated from the activated sludge in the two bioreactors on Illumina HiSeq2000 platform. Three kinds of sequences (16S rRNA amplicons, 16S rRNA gene tags and predicted genes) were used to conduct taxonomic assignment and their applicabilities and reliabilities were compared. Specially, based on 16S rRNA and amoA gene sequences, AOB were found more abundant than AOA in the two bioreactors. Furthermore, the analysis of the metabolic profiles and pathways indicated that the overall pathways in the two bioreactors were quite similar. However, the abundances of some specific genes in the two bioreactors were different. In addition, 454 pyrosequencing was also used to detect potentially pathogenic bacteria in environmental samples. It was found most abundant potentially pathogenic bacteria in the WWTPs were affiliated with Aeromonas and Clostridium. Aeromonas veronii, Aeromonas hydrophila and Clostridium perfringens were species most similar to the potentially pathogenic bacteria found in this study. Overall, the percentage of the sequences closely related to known pathogenic bacteria sequences was about 0.16% of the total sequences. Additionally, a Java application (BAND) was developed for graphical visualization of microbial abundance data. / published_or_final_version / Civil Engineering / Doctoral / Doctor of Philosophy

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This study defines depositional environments and constructs the sequence stratigraphic framework of the lower Miocene Oakville Formation and the basal part of the middle Miocene Lagarto Formation in the Carancahua Bay area. The Early Miocene of the northwestern Gulf of Mexico represents a tectonically stable period with a high sediment influx. The analysis is based on a data set composed of 45 well logs and 200 mile2 area 3D seismic volume. The study interval was divided into five depositional sequences 1-5 that encompass 0.6-2.5 My. LST, TST, and HST systems tracts were recognized by stacking patterns and bounding surfaces. Sequence thickness increases from sequence 1 to 3 and displays reverse thickness trends from sequence 3 to 5, implying changes in accommodation space relative to sediment supply, beginning with high rates of accommodation and evolving into low accommodation rates relative. Besides type-1 depositional sequence which forms during relative sea-level fall below the shoreline break, regressive units of T-R sequence model were also defined and delineated. The interval contains four regressive units, R-Unit1-4. The R-Unit net sandstone maps exhibit the same characteristic of a dip-oriented source of delta-plain origin and a delta-front depocenter basinward. Integration of well log patterns, sandstone dispersal trends from net sandstone maps and seismic stratal slices led to interpretation of depositional environments in each sequence. LST deposits are represented mainly by incised-valley fill facies. TSTs are composed predominantly of retrogradational barrier/tidal-inlet facies, whereas other TSTs contain lagoonal and reworked deltaic systems. HST1 is composed mainly of fluvial-dominated deltaic systems, whereas deltaic systems in other HSTs exhibit wave-influenced deltaic and strandplain depositional systems. The integrated methodology reveals depositional facies variations in contrast to previous work that interpreted these deposits as shorezone systems. During LSTs coarse-grained sediments bypassed shelf through incised valley systems to a downdip depocenter. More sandy sediments were stored on shelf as deltaic and strandplain deposits during HSTs. In contrast to the others, destructive process occurred in TSTs and reworked sandy sediments, for example from delta fronts to barrier bar and lagoonal facies. Submarine fans form by sediments transported through incised-valley systems and delta fronts are commonly good reservoirs. Hence, presence of such depositional facies in the study area might be genetically linked to exploration targets. / text

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / Statistics / Master / Master of Philosophy