Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.

published_or_final_version / Civil and Structural Engineering / Master / Master of Philosophy

Despite the technology advancement, degradation of water quality due to stormwater continues to be a significant threat to the water and ecosystems due to the exponential growth of industries and agricultural enterprises that discharge stormwater. These anthropogenic activities are the sources of high nitrogen and phosphorus quantities in stormwater, which is responsible for eutrophication phenomena and deterioration of public health. Floating Treatment Wetlands (FTWs) are a potential solution to this problem. Both microcosm and mesocosm level studies were conducted for the effective removal of nutrients in stormwater wet detention ponds with different sorption media under varying nutrient concentrations and weather conditions. Water depth, percent area coverage of the FTWs and littoral zone emergent plants were varied in order to determine nutrient removal efficiency before implementing in an actual pond. Focus has also been placed on the observations of macrophyte-epiphyte-phytoplankton interactions in order to understand temporal characteristics of ecological phenomena. Water quality parameters included Total Nitrogen, Total Phosphorus, Orthophosphate, Nitrate-Nitrogen, and Ammonia-Nitrogen in addition to in-situ parameters such as pH, Dissolved Oxygen, Temperature and Chlorophyll-a. Results clearly indicate that an FTW filled with sorption media of 80% expanded clay and 20% tire crumb can significantly promote the biomass growth. Different levels of nutrient concentrations did affect the plants’ growth and cold temperature in late winter was detrimental to growth. To make the system more viable irrespective of the seasonal weather conditions, the adoption of mixed vegetation is highly recommended in the FTWs implementation. It is also recommended that, the positioning of the floating wetlands should not be in the vicinity of the outlet of the pond as assimilated nutrient under the mat might increase the nutrient concentration in the discharged water. Finally, One-way ANOVA test is performed to check whether or not iv these grouped microcosms and mesocosms with differing experimental setup can be deemed statistically significant

Submitted in complete fulfillment for the degree of Doctor of Philosophy (Biotechnology) in the Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa, 2016. / Many households in developing countries are still without proper sanitation systems. The problems are even more prevalent in rural communities where there are no septic systems in place for the treatment of wastewater. This has resulted in the urgent need for the development and implementation of innovative wastewater treatment systems that are inexpensive, environmental friendly and are able to reduce contaminants to levels that pose no harm to the communities. Constructed rhizofiltration systems have been explored for this purpose. They have been used for many decades in many countries with varying degrees of success at the primary, secondary and tertiary levels of wastewater treatment. Poor optimization of this technology has been due to limited information available about the roles played by the whole system as well as by each component involved in the treatment technology. The current work elucidates the role played by macrophytes and microbial biofilms in the removal of nutrients in the rhizofiltration system. Factors affecting waste removal as well as environmental friendliness of the system were also investigated. The rhizofiltration system was constructed in Durban and was divided into planted (planted with Phragmites australis and Kyllinga nemoralis) and unplanted (reference) section. Dissolved oxygen (DO), pH, water temperature, total dissolved solids (TDS), electrical conductivity (EC) and salinity were monitored. The removal efficiency of nutrients was measured using spectrophotometric methods by measuring the concentration of ammonia, nitrate, nitrite, phosphate and orthophosphate in the wastewater pre- and post-treatment. The total organic carbon, chemical oxygen demand (COD), total Kehldjahl nitrogen, biological oxygen demand (BOD), ammonia, nitrate and the flow rate of wastewater into the system from the settling tank were used for the estimation of carbon dioxide, methane and nitrous oxide emitted from the rhizofilter using the 2009 EPA formulae. Both the planted and reference sections of the system removed nutrients with varying efficiencies. The reduction of nutrients in the rhizofilter was found to be seasonal, with most nutrients removed during the warm seasons. The system also retained more nutrients when wastewater containing low levels of nutrients was used. The unpaired t-test was used to determine the differences between nutrient removals between planted and reference sections. Higher reduction efficiencies of nutrients were obtained in the planted section. Up to 65% nitrite and 99% nitrate were removed while up to 86% total phosphorus was removed in a form of orthophosphate (86%). Removal of total nitrogen was shown to increase under high temperature conditions, while the same conditions decreased the total phosphorus removal. High temperatures also increased the performance of the system. The reduction of nutrients in the system corresponded to reduction of the chemical oxygen demand which also positively correlated to the dissolved oxygen concentration. Considering the discharge limits for all nutrients, the discharges in the effluent of the planted section were within the allowable limits as per South Africa’s Department of Water affairs and Forestry in 2012 but not in 2013. The results obtained in 2013 were due to increased nutrient loading introduced into the system. Diverse microbial communities occurred in the treatment system, with more diversity in the planted section. These organisms were supported by macrophytes in the planted section, and were responsible for nitrogen and phosphorus transformation. This explains why total nitrogen and phosphorus reduction was higher in the planted compared to the reference section. Both the planted and the reference sections of the rhizofiltration system produced the greenhouse gases. When the two sections were compared, the planted section produced more gases. Gases emitted by both sections were lower when compared to emission from sludge treatment reed beds and other conventional systems of wastewater treatments. These findings indicated that constructed rhizofiltration is a cleaner form of waste treatment, producing significantly less greenhouse gases and affecting less of a climate change. Findings of this work have revealed that rhizofiltration technology can be used as a low-cost alternative technology for the treatment of wastewater, using the combination of macrophytes and microbial biofilms. Macrophytes accumulated nitrogen and phosphorus as well as supported diverse microorganisms that metabolized and reduced nutrients in the rhizofiltration unit. / D

published_or_final_version / Civil Engineering / Doctoral / Doctor of Philosophy

Submitted in fulfilment of the requirements of the degree of Master of Technology: Biotechnology, Durban University of Technology, Durban, South Africa, 2014. / Wastewater treatment plants (WWTPs) frequently experience bulking and foaming episodes, which present operational challenges by affecting sludge settling due to the excessive proliferation of filamentous bacteria. Various control strategies have been implemented over the years to minimize filamentous growth, however, filamentous bulking still remains an unresolved problem in many WWTPs worldwide. The current study focused on developing and optimizing remedial measures viz., specific and non-specific methods to reduce problematic filamentous bacteria in a full-scale WWTP. Specific methods demonstrated the influence of plant operational parameters viz. chemical oxygen demand, influent N-NH4+, food to microorganism ratio, dissolved oxygen, temperature and pH on the abundance of filamentous bacteria. A cumulative logit model was used to determine the significant relationships between the individual filamentous bacteria at present and the prevailing plant operational parameters. Using the above statistical approach, significant observations and predictions were made with respect to the individual filamentous growth under certain operational parameters. With further validation, this model could be successfully applied to other full-scale WWTPs identifying specific parameters that could contribute to filamentous bulking, thus providing a useful guide for regulating specific filamentous growth. Non-specific control methods such as chlorine, ultraviolet irradiation and ozone treatment were investigated on filamentous bacteria using a live/dead staining technique. To achieve at least 50% reduction of filamentous bacteria, a chlorine dose of 10 mg Cl2/L was required, all filaments were killed at a dose of 22 mg Cl2/L. In addition, an effective UV and ozone dose of 4418.91 μw seconds/cm2 and ±20 mg O3/L respectively, was required to kill 50% of the filamentous bacterial population. Among the three non-specific methods, ozone treatment seemed to be an effective method in controlling the filamentous population with a low negative impact to the surrounding environment. This study serves as a useful guide on the problems and control of filamentous bulking in activated sludge plants. / M

Submitted in fulfillment for the requirements for the Degree of Doctor of Technology: Biotechnology, Durban University of Technology, 2012. / Nitrification is a crucial step in biological nutrient removal (BNR) processes, mostly carried out by a group of nitrifying bacteria which includes ammonia-oxidising bacteria (AOB) and nitrite-oxidising bacteria (NOB). Nitrification failure has proven to be a common operational problem in full-scale wastewater treatment plants (WWTP) since nitrifying bacteria are very sensitive to sudden changes in environmental or plant operating conditions. The current investigation was carried out to advance our understanding of the distribution of nitrifying bacterial populations and their performance at three different BNR plants in KwaZulu-Natal, South Africa. The latest molecular techniques such as fluorescent in situ hybridisation (FISH)-confocal scanning laser microscopy (CSLM), polymerase chain reaction (PCR) and real-time quantitative PCR (Q-PCR) were applied to detect and quantify nitrifying bacteria. When using FISH to target the nitrifying population, it necessitated optimising pre-treatment protocols of the samples to improve accuracy during quantification. Sonication was found to be the superior method of dispersion based on the least disruption of nitrifier cell integrity, irrespective of the sludge type. The effect of plant configurations and wastewater characteristics on the distribution of the nitrifying bacterial population and subsequently on the nitrification performance was evaluated using FISH and PCR. FISH results revealed the dominance of Nitrosomonas (AOB), Nitrobacter (NOB) and Nitrospira (NOB) for all BNR plants. The 16S rRNA analysis of PCR products using genus-specific primers, revealed the presence of more than one species of the same group at these plants. Nitrosomonas spp. including Nitrosomonas halophila, Nitrosomonas eutropha, Nitrosomonas europaea, Nitrosomonas aestuarii and an unidentified Nitrosomonas spp. were found to dominate among the AOB and Nitrobacter vulgaris, Nitrobacter alkalicus, Nitrobacter hamburgensis and an unidentified Nitrobacter spp. were the dominant species for NOB. Among these species, Nitrosomonas aestuarii, Nitrosomonas europaea, Nitrobacter hamburgensis were detected only from the industrial wastewater samples. The efficiency of two commonly used techniques viz., FISH and Q-PCR for the detection of nitrifiers from WWTP were also studied and compared, specifically targeting Nitrobacter sp. Even though there were slight variations in the quantification results, changes in the Nitrobacter community at these plants were consistent for both FISH and Q-PCR results. Both techniques have their own limitations and advantages. This study has helped to add to the platform of understanding the distribution and activity of nitrifying bacteria by correlating population dynamics with the operational parameters at full-scale level. The observations made in this study will assist researchers and engineers to minimise future nitrification failure at full-scale BNR plants. This study also confirmed the highly complex activities of wastewater treatment processes, which is dependant on a number of factors. Specific AOB or NOB predominant in wastewater rather suggests that the wastewater type and characteristics may contribute to significantly different microbial environments. Among the AOB, Nitrosomonas dominated at all BNR plants throughout the study period and for NOB both Nitrobacter and Nitrospira were found in significant numbers but their dominance varied across the plants. These dissimilar, distinct distribution patterns could be attributed to their environment which in turn impacted on the nitrification performance of the system. It was also noted that the co-existence of more than one group of these communities at the same plant could help the plant escape complete functional failures such as nitrification, due to sudden changes in temperature and substrate concentrations, as this function can be performed by different groups. Although it would have been meritorious to conduct a nitrogen balance in this study, this was not possible since the research focused on full-scale systems. / National Research Foundation / D

University of Macau / Faculty of Science and Technology / Department of Civil and Environmental Engineering

The subject of water scarcity and the rate of water consumption has become popular topics over the last few decades. It is possible that society may consume or contaminate much of the remaining readily available water if there is not a paradigm shift. This deep rooted concern has prompted investigations to identify alternative water use and treatment methods. Within this report, information is presented from the use of innovative water harvesting and on-site sewage treatment and disposal systems (OSTDS) at Florida’s Showcase Green Envirohome (FSGE.net), while also addressing low impact development (LID) practices. FSGE is a residential home that demonstrates methods that use less water and reduce pollution. Population increases have more than just an effect on the volume of water demanded. Adverse impacts on surface and groundwater quality are partially attributed to current design and operation of OSTDS. Nutrient loading from wastewater treatment systems may be a concern where numerous OSTDS are located within nutrient sensitive environments. Groundwater nitrate concentrations have been shown to exceed drinking water standards by factors of three or greater surrounding soil adsorption systems (Postma et al., 1992, Katz, 2010). As a contribution to efforts to reduce water use and improve water quality, this study investigates the viability and effectiveness of a residential integrated stormwater, graywater, and wastewater treatment system (ISGWTS) installed and operating for over a year at FSGE. ii Within this report is a continuation of results published previously that consisted of preCertificate of Occupancy (pre-CO) data and an optimization model at the Florida’s Showcase Green Envirohome (FSGE) in Indialantic, Florida (Rivera, 2010). This current report contains 12 months of post-CO data, along with data from bench scale models of the on-site septic treatment and disposal system (OSTDS). There are two main objectives of the study. The first objective is to quantify the performance of the passive treatment Bold & GoldTM reactive filter bed (FDOH classified “innovative system”) for nutrient removal. The second objective was to monitor the water quality of the combined graywater/stormwater cistern for non-potable use and assess the components (green roof, gutters, graywater piping). The performance of the passive innovative system is compared to past studies. Also a bench scale model that is constructed at the University of Central Florida (UCF) Stormwater Management Academy Research and Testing Lab (SMART Lab) is operated to provide data for two different retention times. Complex physical, biological, and chemical theories are applied to the analysis of wastewater treatment performance. The data from the OSTDS and stormwater/graywater cistern are assessed using statistical methods. The results of the OSTDS are compared to FDOH regulatory requirements for “Secondary Treatment Standards”, and “Advanced Secondary Treatment Standards” with promising results. The bench scale results verify that both nitrogen and phosphorus removal are occurring within the filter media and most likely the removals are due to iii biological activity as well as physiochemical sorption. The flow into the OSTDS has been reduced with the use of separate gray water system to only 29 gallons per person per day (gpcd). After the FSGE certificate of occupancy and for one year using the Bold & Gold Biosorption Activated Media (BAM), the TSS, BOD5, and CBOD5 are below the required 10 mg/L for the FDOH classified Advanced Secondary Treatment Systems. The effluent for the conventional drain field TSS, BOD5, and CBOD5 are above 10 mg/L (29.6, 35.7, and 29.0 mg/L). The effluent total nitrogen and total phosphorus for the innovative system are 29.7 mg/L and 4.1 mg/L, which are not low enough for the 20 mg/L nitrogen requirements, but are below the 10 mg/L phosphorus requirements. The conventional drain field has an effluent total nitrogen concentration of 70.1 mg/L and an effluent total phosphorus concentration of 10.6 mg/L, which both fail to meet FDOH Advanced Secondary Treatment requirements. The high nitrogen in the effluent can be attributed to high influent concentrations (about 3 times the average at about 150 mg/L). Longer residence times are shown to produce a removal greater than 90%. Also, nitrate average levels were below the 10 mg/L standard. The combined stormwater/graywater cistern is analyzed against irrigation standards. The graywater is filtered and disinfected with ozone to provide safe water for reuse. Nutrient concentrations are measured to compare with regulatory standards. For irrigation standards, salinity in the form of sodium, calcium, and magnesium are measured. Although high sodium adsorption ratio (SAR) and electrical conductivity (EC) values were recorded, their adverse iv impact on the vegetation has not been observed. . The only observed effect within the home to date is scale formation in the toilet. The use of potable water in FSGE is reduced to 41 gpcd using the integrated stormwater and graywater system. A minor volume of backup artesian well water was added to the cistern during the one year home occupancy phase. Based on less use of potable water and at the current potable water cost rate, the integrated stormwater and graywater system at FSGE will save the typical homeowner about $215 per year. If irrigation were used more often from the cistern, the cost savings in reduced potable water used for irrigation would increase the savings. The treatment cost for B&G BAM over a 40 year period of time based on a flow of 29 gpcd (as measured at FSGE) and for 4 persons is $2.07 per thousand gallons treated. The yearly cost of treatment is about $87.65. There is a reduction in potable water use estimated at 64% of the sewage flow (or 18.5 gpcd) which equates to about 27 thousand gallons in one year. The current average cost of potable water is $4.40 per thousand gallons. Based on reduced potable water usage, the savings per year are about $118.84. Thus the yearly savings in potable water cost ($118.84) offsets the cost of OSTDS treatment at FSGE for nutrient control ($87.65) using the data collected at FSGE. This comparison does not include the inflation cost of water over time. There is also an environmental preservation intangible cost (not quantifiable from this study) from reduced surface runoff and reduced pollutant discharges.

M. Tech. (Department of Biotechnology, Faculty of Applied and Computer Sciences) Vaal University of Technology. / Pollution of aquatic systems by wastewater containing pathogens, heavy metals and high concentrations of nutrients is of great concern due the ecological risks they impose. The toxic effects of metals may occur even at low concentrations because of potential bio magnification in the food chain. Excessive nutrients cause algal blooms which depletes oxygen and prevents sunlight from penetrating into the water, thereby killing fish and other aquatic organisms. This study investigated the pollutant removal efficiency of a riparian wetland located in Sebokeng, Emfuleni local municipality, South Africa. The study was carried out to assess the water quality of a wetland located downstream of the Sebokeng wastewater treatment plant by monitoring and analysing the physico-chemical parameters which included pH, temperature, electrical conductivity, nutrient levels (nitrates, phosphates, nitrites) and heavy metals. The water samples were collected from the effluent discharge of the treatment plant, upstream and downstream of the wetland. Plant uptake of heavy metals in a riparian wetland, nitrification as well as denitrification processes have been historically recorded as the main processes that contribute to the high removal of pollutants in a wetland. The contaminant concentrations of the influent and the effluent were used to estimate the wetland efficiency in improving the water quality that passes through it and its potential effects on improving the quality of irrigation waters. The heavy metals of interest included Al, Cd, Cr, Cu, Fe, Pb, Mn and Zn. Most heavy metals within the wetland occurred at low concentrations (lower than detectable limits and within the discharge limits for irrigation purposes). The results indicate that the average removal efficiencies for Electrical Conductivity (EC), Total coliforms (TC), E. coli, BOD5, COD, TSS, carbonate hardness, aluminium, iron, manganese, copper, nitrite, nitrate, sulfate and ortho-phosphate were 43 %, 51%, 85%, 60%, 61%, 61%, 21%, 67%, 52%, 51%, 83%, 56%, 89%, 49% and 54% respectively. The study showed that this wetland can provide up to 89% removal efficiency of pollutants. Of particular significance was the high pathogen and nutrient removal efficiency. A t-test was performed in order to determine the statistical significance of the wetland pollutant removal efficiencies. All p-values calculated were well below 0.05 and the removal efficiencies are therefore considered statistically significant. For this particular ecosystem the findings show that there is no great concern about metal pollution since most of the metals tested for were below the minimum limit for irrigation stipulated by the South African water regulation department (DWAF 1996a). Therefore, the wetland effluent water qualifies for both agriculture and landscape irrigation. Future considerations in choosing to use wetlands as a polishing facility for wastewater treatment systems are highlighted in the study.