Multiplex polymerase chain reaction (PCR) assays were designed that incorporated primer pairs for the sex determining region on the Y chromosome, Sry, and one or two control sequences. A triplex and a duplex assay were created involving Sry and Dax, a single copy X chromosome gene which is involved in the female sex determination pathway. The third sequence in the triplex assay was a repetitive Y chromosome sequence, YR. A minimum of 2.5 x 10-3 to 2.5 x 10 -4 m g/ m L of male DNA and 2.5 x 10-5 m g/ m L of female DNA was required if a single multiplex PCR was performed. To demonstrate that sex determination of preimplantation porcine embryos is possible, morulae were collected 5 d post insemination from pregnant mare serum gonadotrophin (PMSG)/human chorionic gonadotrophin (hCG)-treated 60--70 kg prepubertal gilts. Embryos were biopsied using a micromanipulator and cells were placed into individual microcentrifuge tubes for further analysis. Embryos were then cultured to the blastocyst stage. A total of 315 embryos were sexed via the PCR assay with a resultant female/male (%) ratio of 73/27. When 94 embryos from heavier gilts (90--100 kg) were sexed in a similar assay, the resultant female/male ratio was 60/40. Attempts were made to correlate these results with karyotypes. Five transfers of sexed embryos into synchronized recipients were attempted. None of these resulted in pregnancies; although return to estrus was delayed by two to eight days, in four out of the five recipients. Our findings suggest that PCR amplification of the Sry gene can be a reliable method for sexing porcine embryos. It does appear that embryo quality is critical for both the PCR assay and subsequent successful culture. (Abstract shortened by UMI.)

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Typescript. / Bibliography: leaves 166-171. / Microfiche. / xiv, 171 leaves, bound ill. 29 cm

Thesis (M.S.)--Michigan State University. School of Criminal Justice , 2006. / Title from PDF t.p. (viewed on June 19, 2009) Includes bibliographical references (p. 81-84). Also issued in print.

Thesis--University of Michigan, 1931. / Cover title. "Papers from the Department of Botany, University of Michigan, no. 359." "Reprinted from the American journal of botany, vol. XIX, no. 4 ... April, 1932." "Literature cited": p. 381-383.

Thesis--University of Michigan, 1931. / Cover title. "Papers from the Department of Botany, University of Michigan, no. 359." "Reprinted from the American journal of botany, vol. XIX, no. 4 ... April, 1932." "Literature cited": p. 381-383.

Thesis (M.Med.Sc.)--University of Hong Kong, 2003. / Includes bibliographical references (leaves 53-57). Also available in print.

Inaug.-Diss.--Heidelberg. / Lebenslauf. "Benutzte literatur": p. [21]-22.

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

Thesis (MSc.(Anatomy)--Faculty of Health Sciences)-University of Pretoria, 2008. / Includes bibliographical references.