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Sex diagnosis of preimplantation porcine embryos through PCR amplification of the Sry gene / Sex determination of pig embryosWatt, Heather Lynn. January 1998 (has links)
Multiplex polymerase chain reaction (PCR) assays were designed that incorporated primer pairs for the sex determining region on the Y chromosome, Sry, and one or two control sequences. A triplex and a duplex assay were created involving Sry and Dax, a single copy X chromosome gene which is involved in the female sex determination pathway. The third sequence in the triplex assay was a repetitive Y chromosome sequence, YR. A minimum of 2.5 x 10-3 to 2.5 x 10 -4 m g/ m L of male DNA and 2.5 x 10-5 m g/ m L of female DNA was required if a single multiplex PCR was performed. To demonstrate that sex determination of preimplantation porcine embryos is possible, morulae were collected 5 d post insemination from pregnant mare serum gonadotrophin (PMSG)/human chorionic gonadotrophin (hCG)-treated 60--70 kg prepubertal gilts. Embryos were biopsied using a micromanipulator and cells were placed into individual microcentrifuge tubes for further analysis. Embryos were then cultured to the blastocyst stage. A total of 315 embryos were sexed via the PCR assay with a resultant female/male (%) ratio of 73/27. When 94 embryos from heavier gilts (90--100 kg) were sexed in a similar assay, the resultant female/male ratio was 60/40. Attempts were made to correlate these results with karyotypes. Five transfers of sexed embryos into synchronized recipients were attempted. None of these resulted in pregnancies; although return to estrus was delayed by two to eight days, in four out of the five recipients. Our findings suggest that PCR amplification of the Sry gene can be a reliable method for sexing porcine embryos. It does appear that embryo quality is critical for both the PCR assay and subsequent successful culture. (Abstract shortened by UMI.)
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Sex diagnosis of preimplantation porcine embryos through PCR amplification of the Sry geneWatt, Heather Lynn. January 1998 (has links)
No description available.
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Sex change in the endemic Hawaiian labrid Thalassoma duperrey (Quoy and Gaimard) : a behavioral and ecological analysisRoss, Robert Melvin January 1982 (has links)
Typescript. / Bibliography: leaves 166-171. / Microfiche. / xiv, 171 leaves, bound ill. 29 cm
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A more sensitive sex determination assayJackson, Carrie Beth. January 2006 (has links)
Thesis (M.S.)--Michigan State University. School of Criminal Justice , 2006. / Title from PDF t.p. (viewed on June 19, 2009) Includes bibliographical references (p. 81-84). Also issued in print.
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Sporogenesis and sex determination in Begonia schmidtianaPastrana, Maria D., January 1900 (has links)
Thesis--University of Michigan, 1931. / Cover title. "Papers from the Department of Botany, University of Michigan, no. 359." "Reprinted from the American journal of botany, vol. XIX, no. 4 ... April, 1932." "Literature cited": p. 381-383.
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Sporogenesis and sex determination in Begonia schmidtianaPastrana, Maria D., January 1900 (has links)
Thesis--University of Michigan, 1931. / Cover title. "Papers from the Department of Botany, University of Michigan, no. 359." "Reprinted from the American journal of botany, vol. XIX, no. 4 ... April, 1932." "Literature cited": p. 381-383.
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Questionnaire survey on the maternal wish to know the fetal sex from obstetric ultrasound examinationLee, Young-king, John. January 2002 (has links)
Thesis (M.Med.Sc.)--University of Hong Kong, 2003. / Includes bibliographical references (leaves 53-57). Also available in print.
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Kohabitationstermin und Geschlecht des Kindes, nach den fällen der Heidelberger Universitäts-frauenklinik im Kriegsjahr 1916/17 ...Rheinboldt, Meta, January 1918 (has links)
Inaug.-Diss.--Heidelberg. / Lebenslauf. "Benutzte literatur": p. [21]-22.
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Questionnaire survey on the maternal wish to know the fetal sex from obstetric ultrasound examination李揚敬, Lee, Young-king, John. January 2002 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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An anatomical assessment of brain infarcts a MRI study /Potgieter, Janeane January 2008 (has links)
Thesis (MSc.(Anatomy)--Faculty of Health Sciences)-University of Pretoria, 2008. / Includes bibliographical references.
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