Construction practices are constantly evolving in order to adapt to physical locations and economic conditions. These adaptations may result in more cost-effective designs, but may also come at a cost of strength. In masonry shear walls, it is becoming more common to reduce the amount of grouting from every cell to only those with reinforcement, a practice known as partial-grouting. Partially-grouted masonry responds differently and in a more complex matter to lateral loads as compared to fully-grouted masonry. The response is made even more complex by wall discontinuities in the form of openings. The main objective of this study is to validate the strut-and-tie procedure for the in-plane lateral strength prediction of partially-grouted, multistory, reinforced concrete masonry walls with openings. The research included testing six three story, half-scale masonry shear walls. Half of the walls had door openings while the other half had window openings. The configurations were selected to represent typical walls in multi-story buildings. The measured lateral strength was compared to estimations from the equations in the US masonry code and to those from an equivalent truss model and a strut-and-tie model. The results show that the U.S. masonry code equations over predicts while the equivalent truss model under predicts the lateral strength of the walls. The results further show that the strut-and-tie model is the most accurate method for lateral strength prediction and is able to account for wall openings and partial-grouting.

Masonry is one of the oldest forms of construction materials that is still in use today. However, construction practices in the modern age demand faster and more economical practices. Dry-stack masonry, or masonry that doesn't use mortar to bind the blocks together, is a unique system to make masonry more economical. Though several systems of dry-stack masonry have been suggested little to no data exists as most of these systems are patented. This research used dry-stacked normal weight concrete masonry units with an eccentrically placed reinforcement. The wall system is connected through a surface bond and lacks any geometric connection. Previously, research has been conducted on the wall system for its axial compressive capacity, but little information is known about its ability to withstand lateral forces such as earthquakes. Research was conducted on the wall system in order to determine the seismic parameters, including the force reduction factor, overstrength factor, and the displacement amplification factor. To determine these factors the guidelines from the Federal Emergency Management Agency (FEMA) Quantification of Building Seismic Performance Factors 2009 were followed. The guidelines are explicit that both experimental data and computer modeling are needed to quantify these parameters. Experimental data was obtained from a diagonal tension test, and an in-plane shear test. The diagonal tensions test provided preliminary values on the shear modulus and shear resistance. The in-plane shear test was of primary interest and what would be used to verify the computer model. Computer modeling of the wall system was accomplished with Vector 2. Initially the computer modeling was done to reproduce experimental data. Then, a parametric study was performed using the model to see what component of the wall most effected its capacity. This analysis showed that the surface bond was the component of the wall that most affects its capacity. Finally, the computer model was run through the FEMA Far-Field earthquake suite to gather data on the strength and ductility. Values of the force reduction factor, overstrength factor, and displacement amplification factor were determined based on the time history analysis and pushover analysis on the computer model.

Cancer cells traveling to distant tissues during metastasis must survive passing through the circulation. However, the influence of this fluid microenvironment on these cells is poorly understood. It was previously viewed that exposure to the hemodynamic shear forces within circulation was inhospitable to cancer cells, causing the cells to be destroyed. Recent evidence indicates that transformed cells are markedly more resistant to fluid shear stress when compared to non-transformed epithelial cells. Furthermore, these cells selectively adapt following exposure to fluid shear stresses and become more resistant to subsequent exposures to shear stress. The mechanisms behind this difference in phenotype and induced resistance are investigated. The elastic modulus, a measure of stiffness, may play a role in resistance and is shown to be altered upon exposure to fluid shear forces. Additionally, plasma membrane repair is a critical process in the resistance phenotype as cells sustain damage but are able to maintain viability. Cytoskeletal dynamics are also shown to play a role in resistance to fluid shear forces.

There is increasing interest in assessing the role of hemodynamics in aneurysm growth and rupture mechanism. Identification of the indicators of rupture risk can prove very valuable in the clinical management of patients. If rupture risk of aneurysms can be predicted, immediate preemptive treatments can be done for the high risk patients whereas others can avoid the risky intervention. Retrospective studies have been performed in the past to filter out indices that differentiate ruptured aneurysms from unruptured aneurysms. However, these differences may not necessarily translate to differences between aneurysms that present unruptured but fork towards growth/rupture and unruptured aneurysms that are invariably stable. The hypothesis of the present study is that hemodynamic indices of unruptured aneurysms when they first presented can be used to predict their longitudinal outcome. A prospective longitudinal cohort study was designed to test this hypothesis. Four clinical centers participated in this study and a total of 198 aneurysms were recruited. These aneurysms were chosen by the physicians to be kept under watchful waiting. Three-dimensional models of aneurysms and their contiguous vasculature generated using the initial scans of patients were used for computational fluid dynamic (CFD) simulations. Both pulsatile and steady flow analyses were performed for each patient. By collating all the prominent hemodynamic indices available in aneurysm literature and developing a few new indices, 25 hemodynamic indices were estimated for each subject. For statistical analysis, it was hypothesized a priori that low wall shear area is different between stable and unstable aneurysms. All other indices were tested in a post-hoc manner. The longitudinal outcome information of these patients was recorded at the clinical centers and the author was blinded until all analyses were complete. Aneurysms that grew during the follow up period were labeled as "grown" and otherwise they were called "stable" by the radiologists. After the hemodynamic analysis was complete, a non-parametric Mann Whitney U test was performed to determine if any index can statistically differentiate the two groups ("grown" versus "stable"). It was found that none of the indices distinguished the two groups with statistical significance. Comparison of the steady and pulsatile flow analysis suggested that the patient population is stratified in the same order by an index, irrespective of whether the index is computed using a steady or pulsatile flow simulation. Pearson correlation coefficient was obtained between basic geometric indices and hemodynamic indices of this population. No strong correlation was found in between morphology and hemodynamics, suggesting uniqueness of the hemodynamic indices. The hypothesis motivating the present study is that aneurysm blood flow based indices can be used as prognostic indicators of growth and/or rupture risk. This study is the first to analyze intracranial aneurysm hemodynamics of a large cohort in a longitudinal prospective manner. Results of the present study indicate that quantitative hemodynamics cannot be used to predict the longitudinal outcome of an aneurysm. Further studies are needed to gain additional clinical insights.

This study developed a nonlinear constitutive model for a sustainable orthotropic material. Existing methods for constitutive models of wood were improved upon to include the nonlinear stress-strain response not only in the two orthogonal axes but at any orientation to the strong axis of the material. This method also simplifies the nonlinear stress-strain relationships into bilinear stress-strain curves which can be valuable in hand calculations as well as finite-element analyses. The effectiveness of the proposed constitutive model is demonstrated by comparing bilinear stress-strain predictions to experimental data.

The objective of this thesis is to determine the relative shear strengths of halloysite, kaolinite, synthetic mixtures, and local soils, to investigate the influence of halloysite content on the shear strength of kaolinite, and to explore the possibility that the strength properties of soil clays might be controlled by the relative content of their component minerals.

Scientists have been well aware of the complexity of Martian and lunar regoliths. There are vast unexplored areas on both, the Moon and Mars, as well as uncertainties in our understanding of the physicochemical properties of their regoliths. Lunar and Martian regoliths differ from terrestrial soils in that they appear granular, but are expected to contain some cohesion. As such, cohesion in regolith poses challenges for future space operations, more specifically for landing, settlement, and mobility purposes. The ability to induce prescribed levels of cohesion in regolith simulants and reliably measure it would allow scientists to evaluate space technology limitations under different operational scenarios on Earth prior to a mission. Therefore, the objectives of this research were to (1) develop methods to induce prescribed levels of cohesion in dry granular media, and (2) evaluate accessible and reliable testing methods to measure cohesion. We developed and evaluated several methods to induce cohesion in two types of dry sand, F-75 silica sand and generic play sand. The methods to induce cohesion included play sand mixed with sugar-water, polymeric sand, and nanocellulose fibers, as well as F-75 sand mixed with polydimethylsiloxane, polyvinyl acetate, crystalline silica, agar, zero-valent iron, adhesive spray, and sand surface modification using a plasma gun. Each method was assessed for advantages and disadvantages, and laboratory specimens produced using the most promising methods were tested at different compositions and densities to measure cohesion. The laboratory methods used to measure the cohesion included direct shear test, simple direct shear test, and vertical cut test. The results from these tests were then compared to tensile strength tests, using a split box test. In addition, these tests were also performed on lunar simulants JSC-1A and GRC-3 at different densities. The direct shear apparatus was available, but the other three devices were fabricated as part of this work. Based on the research results, simple methods to potentially induce low levels of cohesion in dry granular media are suggested along with suitability of laboratory methods to measure the added cohesion.

The George Sound Shear Zone (GSSZ) exposed in Bligh Sound within Fiordland, New Zealand allowed us to reconstruct the kinematics of transpressive flow in >100 km2 of exhumed Cretaceous lower crust. We compare the three-dimensional characteristics of the deformation to theoretical models of transpression that assume steady-state flow in a homogeneous medium. This assumption is rarely the case for shear zones that experience metamorphism during deformation. We determined the three-dimensional kinematics of the GSSZ and evaluated the effects of metamorphism on strain accommodation and structural fabric evolution in the GSSZ to determine if metamorphism is an important parameter that transpressional models should account for. We found that metamorphism aided strain localization within the GSSZ and resulted in a style of structural fabric development that deviates from predictions made by theoretical models. We used foliation and lineation orientation data and field observations to determine GSSZ kinematics. Asymmetric pyroxene σ-porphyroclasts and hornblende fish show top-down-to-the-SW apparent normal shear sense with a sinistral component. The Z-axes of oblate SPO ellipsoids define the vorticity normal section and the moderately WNW-plunging vorticity vector. Foliation deflections relative to the shear zone boundaries yielded a vorticity magnitude (Wk) of ≥0.8. Our kinematic results suggest that the GSSZ records inclined, triclinic transpression with sinistral, top-down-to-the-SW simple shear-dominated flow. We used finite strain analysis and petrographic analysis to determine that metamorphism influences strain accommodation. Finite strain analyses were performed in 3D on 16 samples using the Rf/ɸ, Fry, and Intercept methods to determine the SPO fabric ellipsoids at different stages of deformation. Petrographic analysis was performed to identify metamorphic reactions using syn-kinematic minerals and constrain deformational temperatures using deformation mechanisms of plagioclase. Early deformation formed a ~13 km wide prolate fabric at granulite facies. Deformation later localized into a ~2-4.6 km wide oblate, mylonitic fabric at upper amphibolite facies. This fabric cross-cuts the prolate fabric and is characterized by metamorphic hornblende and biotite produced from retrogressive hydration reactions. Samples with syn-kinematic biotite contain more shear bands and display more grain size reduction of plagioclase than samples without this phase, suggesting these samples may have accommodated more strain. Changes in syn-kinematic metamorphic minerals were accompanied by steepening of stretching lineations and by changes in foliation orientation. Our analyses show that retrogressive hydration metamorphism aided strain localization within a cross-cutting oblate fabric, and the uneven distribution of biotite within this domain potentially influenced along strike variation in strain magnitude and fabric ellipsoid symmetry. Our results highlight the influence of fluid-induced metamorphism on shear zone evolution and call for new transpressional models to incorporate changes in rheology due to syn-kinematic metamorphism.

There is a clinical need for large numbers of phenotypes which are suitable for tissue engineering and cell therapy applications. Pluripotent stem cells (PSCs) are readily expanded in vitro and can differentiate into any somatic phenotype, making them a potential cell source. However, generating clinically-relevant numbers of phenotypes requires culture in stir-based bioreactor systems which expose cells to shear stress. Here we use a parallel plate bioreactor as a surrogate model system to better understand the effects of shear stress on pluripotent embryonic stem cells (ESCs). Initial studies examined the impact of cell deformation by shear stress during early ESC differentiation. Shear-treatment regulated specification into the three germ lineages and promoted mesodermal differentiation. Next we examined the response to shear stress during later specification events. The application of shear stress was found to promote mesodermal differentiation towards both definitive hematopoietic and mature endothelial phenotypes, although delayed applications were less effective at promoting hematopoietic specification. The next studies used low oxygen treatment to study the impact of another differentiation cue in the presence and absence of shear stress. Hypoxia promoted mesodermal phenotypes but the response was highly dependent on the physical microenvironment such as the culture method and the presence of shear stress. The next group of studies examined the impact of shear stress on ESC expansion. ESCs expanded under flow conditions maintained pluripotency but mesodermal specification was regulated in a manner that was dependent on the presence or absence of a ROCK inhibitor. The final studies used small molecule inhibitors to determine the role of specific signaling molecules during the shear-mediated differentiation of ESCs. Although inhibition of ROCK had little effect, inhibition c-SRC, JNK, or ERK modulated the shear-response. These studies highlight the important effects of shear stress during PSC culture and increase the basic science understanding of stem cell regulation by the physical microenvironment. The systematic approach to analyze multiple parameters allows for an improved translation of techniques from the bench-top into large-scale bioprocessing systems. Altogether these studies can inform large-scale differentiation techniques and bioreactor design in order to help establish the cell banks needed for clinical applications. / acase@tulane.edu

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