Lo Ting Sze. / Thesis submitted in: December 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 145-170). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.vii / List of Contents / Contents in Brief --- p.viii / Contents in Detail --- p.ix / List of Figures and Tables / List of Figures --- p.xiii / List of Tables --- p.xvi / Chapter Chapter 1 --- Introduction and literature review General introduction --- p.1 / Chapter 1.1 --- Structural changes in ovary of penaeid shrimp during maturation --- p.3 / Chapter 1.2 --- Biochemical and physiological changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1 --- Variation of protein content --- p.7 / Chapter 1.2.1.1 --- Changes of proteins in ovary of penaeid shrimp during maturation --- p.7 / Chapter 1.2.1.2 --- Biochemical characterization of vitellin --- p.8 / Chapter 1.2.1.3 --- Site of vitellogenin synthesis --- p.10 / Chapter 1.2.2 --- Variation of lipid composition --- p.12 / Chapter 1.2.2.1 --- Role of lipids in ovary of penaeid shrimp --- p.12 / Chapter 1.2.2.2 --- Changes of lipids in ovary of penaeid shrimp during maturation --- p.13 / Chapter 1.2.3 --- Variation of other nutrient contents --- p.16 / Chapter 1.3 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.16 / Chapter 1.3.1 --- Gonad-inhibiting hormone (GIH) --- p.17 / Chapter 1.3.2 --- Crustacean hyperglycemic hormone (CHH) --- p.19 / Chapter 1.3.3 --- X-organ sinus gland complex (XOSG) and CHH neuropeptide family --- p.20 / Chapter 1.3.4 --- Gonad-stimulating hormone (GSH) --- p.22 / Chapter 1.3.5 --- Methyl farnesoate (MF) --- p.23 / Chapter 1.3.6 --- Neurotransmitters --- p.24 / Chapter 1.3.7 --- Other factors --- p.26 / Chapter 1.3.8 --- Androgenic hormone (AH) --- p.27 / Chapter 1.4 --- Ecology and reproductive biology of Metapenaeus ensis --- p.29 / Chapter 1.4.1 --- Identification --- p.29 / Chapter 1.4.2 --- Ecology --- p.29 / Chapter 1.4.3 --- Reproductive biology --- p.30 / Chapter 1.5 --- Objective of research --- p.32 / Chapter Chapter 2 --- "Variation of circulating gonad-inhibiting hormone (GIH) level during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 2.1 --- Introduction --- p.34 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- Experimental animals and serum extraction --- p.35 / Chapter 2.2.2 --- Protein content assay --- p.36 / Chapter 2.2.3 --- SDS-polyacrymide gel electrophoresis (SDS-PAGE) --- p.39 / Chapter 2.2.4 --- Western blot --- p.40 / Chapter 2.2.5 --- Purification of IgG from antiserum --- p.42 / Chapter 2.2.6 --- ELISA assay (indirect ELISA) --- p.42 / Chapter 2.3 --- Results --- p.44 / Chapter 2.3.1 --- Experimental animals --- p.44 / Chapter 2.3.2 --- Purification of anti-GIH IgG --- p.46 / Chapter 2.3.3 --- Optimization of indirect ELISA --- p.46 / Chapter 2.3.4 --- Determination of circulating GIH in haemolymph --- p.51 / Chapter 2.4 --- Discussion --- p.55 / Chapter Chapter 3 --- "Identification of genes differentially expressed during ovarian maturation in the shrimp, Metapenaeus ensis" / Chapter 3.1 --- Introduction --- p.60 / Chapter 3.2 --- Materials and methods --- p.61 / Chapter 3.2.1 --- Outline of methodology --- p.61 / Chapter 3.2.2 --- Experimental animals --- p.62 / Chapter 3.2.3 --- Total RNA extraction --- p.62 / Chapter 3.2.4 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.64 / Chapter 3.2.5 --- DNA electrophoresis by agarose gel --- p.68 / Chapter 3.2.6 --- DNA electrophoresis by polyacrylamide gel --- p.68 / Chapter 3.2.7 --- Preparation of DIG-labeled probe from PCR product --- p.69 / Chapter 3.2.8 --- Checking for DIG-labeling yield --- p.69 / Chapter 3.2.9 --- Excision of cDNA library from ovary of Metapenaeus ensis --- p.70 / Chapter 3.2.10 --- PCR screening of insertion sequence --- p.73 / Chapter 3.2.11 --- Dot-blot --- p.75 / Chapter 3.2.12 --- Dot-blot hybridization --- p.76 / Chapter 3.2.13 --- Growth of cell for plasmid preparation --- p.79 / Chapter 3.2.14 --- Permanent culture preparation --- p.80 / Chapter 3.2.15 --- DNA Sequencing --- p.80 / Chapter 3.2.16 --- RNA formaldehyde denaturing gel electrophoresis --- p.82 / Chapter 3.2.17 --- Northern blot --- p.84 / Chapter 3.2.18 --- Northern hybridization --- p.84 / Chapter 3.2.19 --- 5´ة RACE (rapid amplification of cDNA ends) --- p.88 / Chapter 3.3 --- Results --- p.92 / Chapter 3.3.1 --- Experimental animals --- p.92 / Chapter 3.3.2 --- Total RNA extraction --- p.92 / Chapter 3.3.3 --- RNA arbitrarily primed PCR (RAP-PCR) --- p.92 / Chapter 3.3.4 --- Titer of cDNA library from Metapenaeus ensis ovary --- p.95 / Chapter 3.3.5 --- PCR screening of insertion sequence --- p.95 / Chapter 3.3.6 --- Dot-blot hybridization --- p.99 / Chapter 3.3.7 --- DNA sequencing of differentially expressed genes --- p.96 / Chapter 3.3.8 --- Northern analysis --- p.99 / Chapter 3.3.8.1 --- Housekeeping gene - elongation factor 1α --- p.106 / Chapter 3.3.8.2 --- Translationally controlled tumor protein (TCTP) --- p.106 / Chapter 3.3.8.3 --- Cytoskeletal actin --- p.109 / Chapter 3.3.8.4 --- Keratin --- p.109 / Chapter 3.3.8.5 --- Heat shock cognate 70 kD protein (hsc70) --- p.109 / Chapter 3.3.8.6 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.117 / Chapter 3.3.8.7 --- Arginine kinase --- p.117 / Chapter 3.3.8.8 --- High mobility group 1-like protein (HMGl-like protein) --- p.122 / Chapter 3.3.8.9 --- Nucleoside diphosphate kinase --- p.122 / Chapter 3.4 --- Discussion --- p.122 / Chapter 3.4.1 --- RAP-PCR and dot-blot hybridization --- p.122 / Chapter 3.4.2 --- Functions of differentially expressed genes in ovarian maturation --- p.128 / Chapter 3.4.2.1 --- Translationally controlled tumor protein (TCTP) --- p.128 / Chapter 3.4.2.2 --- Cytoskeletal actin --- p.130 / Chapter 3.4.2.3 --- Keratin --- p.130 / Chapter 3.4.2.4 --- Heat shock cognate 70 kD protein (hsc70) --- p.131 / Chapter 3.4.2.5 --- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) --- p.134 / Chapter 3.4.2.6 --- Arginine kinase --- p.135 / Chapter 3.4.2.7 --- High mobility group 1-like protein (HMGl-like protein) --- p.136 / Chapter 3.4.2.8 --- Nucleoside diphosphate kinase --- p.137 / Chapter 3.4.3 --- Speculation on the molecular changes in ooctyes during ovarian maturation --- p.139 / Chapter Chapter 4 --- General conclusion --- p.142 / References --- p.145

Cui, Ju. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 110-136). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables and Figures --- p.xi / List of Abbreviations --- p.xiii / Chapter 1 General introduction --- p.1 / Chapter 2 Literature review --- p.4 / Chapter 2.1 --- Introduction --- p.4 / Chapter 2.2 --- Structural changes in ovary and thelycum of penaeid shrimp during maturaiton --- p.4 / Chapter 2.3 --- Biochemical changes in ovary of penaeid shrimp during maturation --- p.7 / Chapter 2.4 --- Endocrine control of ovarian maturation in penaeid shrimp --- p.9 / Chapter 2.4.1 --- Peptides --- p.9 / Chapter 2.4.2 --- Steroids --- p.17 / Chapter 2.4.3 --- Terpenoids --- p.19 / Chapter 2.4.4 --- Biogenic amines --- p.21 / Chapter 2.6 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.23 / Chapter 2.6 --- Proteomics --- p.27 / Chapter Chapter 3 --- Identification of proteins differentially expressed during ovarian maturation in the shrimp Metapenaeus ensis --- p.28 / Chapter 3.1 --- Introduction --- p.28 / Chapter 3.2 --- Materials and methods --- p.30 / Chapter 3.2.1 --- Animals --- p.30 / Chapter 3.2.2 --- Histological observation --- p.30 / Chapter 3.2.3 --- Data analysis --- p.31 / Chapter 3.2.4 --- Two-dimensional gel electrophoresis --- p.31 / Chapter 3.2.5 --- Protein isolation and identification by MALDI-TOF MS/MS --- p.35 / Chapter 3.3 --- Results --- p.37 / Chapter 3.3.1 --- General ovarian histology --- p.37 / Chapter 3.3.2 --- Morphometric analysis --- p.41 / Chapter 3.3.3 --- Comparison of proteomic patterns of shrimp ovaries and image analysis --- p.43 / Chapter 3.3.4 --- Identification of differentially expressed proteins in shrimp ovaries by MALDI-TOF MS/MS analysis --- p.48 / Chapter 3.4 --- Discussion --- p.61 / Chapter 3.4.1 --- Identification of proteins by MALDI-TOF MS/MS analysis --- p.61 / Chapter 3.4.2 --- Potential functions of the identified ditterentially expressed proteins in shrimp reproduction --- p.62 / Chapter Chapter 4 --- Characterization of cellular retinoic acid binding protein (CRABP) and retinoic X receptor (RXR) in the shrimp Metapenaeus ensis --- p.69 / Chapter 4.1 --- Introduction --- p.69 / Chapter 4.2 --- Materials and methods --- p.72 / Chapter 4.2.1 --- Experimental animals --- p.72 / Chapter 4.2.2 --- Preparation of total RNA --- p.72 / Chapter 4.2.3 --- Rapid Amplification of 5' and 3' cDNA Ends (RACE) --- p.73 / Chapter 4.2.4 --- Subcloning --- p.75 / Chapter 4.2.5 --- Sequencing --- p.78 / Chapter 4.2.6 --- Phylogenetic analysis --- p.78 / Chapter 4.2.7 --- Reverse transcription (RT) --- p.79 / Chapter 4.2.8 --- Semi-quantitative RT-PCR --- p.79 / Chapter 4.2.9 --- Real-Time RT-PCR --- p.80 / Chapter 4.2.10 --- In situ hybridization --- p.81 / Chapter 4.2.11 --- Tissue culture and in vitro ovary explant assay --- p.83 / Chapter 4.2.12 --- Statistical analysis --- p.84 / Chapter 4.3 --- Results --- p.84 / Chapter 4.3.1 --- Full-length RXR cDNA derivation --- p.84 / Chapter 4.3.2 --- Sequence comparisons and phylogenetic analyses --- p.86 / Chapter 4.3.3 --- Spatiotemporal expression profiles of MeCRABP and MeRXR mRNA in female shrimp --- p.92 / Chapter 4.3.4 --- Effect of exogenous retinoic acids on the expression of MeCRABP and MeRXR in shrimp ovaries --- p.96 / Chapter 4.4 --- Discussion --- p.101 / Chapter 4.4.1 --- Cloning and characterization of the M. ensis RXR --- p.101 / Chapter 4.4.2 --- Developmental expression of CRABP and RXR in shrimp ovary --- p.102 / Chapter 4.4.3 --- Effects of exogenous retinoic acids --- p.104 / Chapter Chapter 5 --- General conclusion --- p.106 / References --- p.110

Mak Wai Yan. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 95-115). / Abstracts in English and Chinese. / Declaration --- p.i / Abstract --- p.ii / Acknowledgements --- p.viii / Table of Contents --- p.ix / List of Tables --- p.xiii / List of Figures --- p.xiv / List of Abbreviations --- p.xvi / Chapter Chapter 1 --- Introduction and Literature Review / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Endocrine control of reproduction in crustaceans --- p.2 / Chapter 1.3 --- The X-organ sinus gland (XOSG) complex --- p.3 / Chapter 1.3.1 --- CHH/MIH/GIH family of neuropeptides --- p.4 / Chapter 1.3.2 --- Gonad inhibiting hormone (GIH) --- p.6 / Chapter 1.3.3 --- Molt inhibiting hormone (MIH) --- p.7 / Chapter 1.3.4 --- Crustaceans hyperglycemic hormone (CHH) --- p.9 / Chapter 1.3.5 --- The chormatophorotropins (RPCH and PDH neuropeptide family) --- p.10 / Chapter 1.4 --- Other mechanisms of reproduction control --- p.11 / Chapter 1.4.1 --- Methyl farnesoate (MF) and mandibular organ inhibiting hormone (MOIH) --- p.11 / Chapter 1.4.2 --- Gonad stimulating hormone (GSH) --- p.13 / Chapter 1.4.3 --- Serotonin (5HT) --- p.13 / Chapter 1.4.4 --- Dopamine --- p.14 / Chapter 1.4.5 --- Enkephalin (ENK) --- p.15 / Chapter 1.4.6 --- 17β-Estradiol --- p.16 / Chapter 1.4.7 --- Progesterone --- p.17 / Chapter 1.5 --- Androgenic hormone (AH) --- p.18 / Chapter 1.6 --- Objective and methodology of present research --- p.19 / Chapter 1.7 --- Reproductive biology of the shrimp Metapenaeus ensis --- p.20 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Introduction --- p.26 / Chapter 2.2 --- Extraction of eyestalk RNA from the shrimp --- p.27 / Chapter 2.3 --- Construction of the eyestalk cDNA library --- p.28 / Chapter 2.3.1 --- First-strand cDNA synthesis --- p.28 / Chapter 2.3.2 --- cDNA amplification by LD PCR --- p.29 / Chapter 2.3.3 --- Proteinase K digestion --- p.29 / Chapter 2.3.4 --- Sfi I digestion --- p.30 / Chapter 2.3.5 --- cDNA size fractionation --- p.30 / Chapter 2.3.6 --- Ligation of cDNA to λ TriplEx2 Vector --- p.31 / Chapter 2.3.7 --- Packaging --- p.31 / Chapter 2.3.8 --- Titering the unamplified library --- p.32 / Chapter 2.3.9 --- Library amplification --- p.33 / Chapter 2.3.10 --- Titering the amplified library --- p.34 / Chapter 2.4 --- Mass Excision of the eyestalk cDNA library --- p.34 / Chapter 2.5 --- PCR screening of inserted sequence --- p.35 / Chapter 2.6 --- RNA arbitrarily primed polymerase chain reaction (RAP-PCR) --- p.35 / Chapter 2.7 --- Dot blot hybridization --- p.37 / Chapter 2.7.1 --- Probe Labelling --- p.37 / Chapter 2.7.2 --- Dotting of membrane --- p.38 / Chapter 2.7.3 --- Hybridization --- p.38 / Chapter 2.7.4 --- Washing and chemiluminescent detection --- p.39 / Chapter 2.7.5 --- Probe stripping for re-hybridization --- p.40 / Chapter 2.8 --- Sequencing --- p.40 / Chapter 2.9 --- BLAST search --- p.41 / Chapter 2.10 --- Northern blot analysis --- p.41 / Chapter 2.10.1 --- Probe labelling --- p.41 / Chapter 2.10.2 --- RNA formaldehyde denaturing gel electrophoresis --- p.41 / Chapter 2.10.3 --- Northern blot --- p.42 / Chapter 2.10.4 --- Pre-hybridization --- p.43 / Chapter 2.10.5 --- Hybridization --- p.43 / Chapter 2.10.6 --- Washing and chemiluminescent detection --- p.43 / Chapter 2.11 --- Real-time RT-PCR --- p.44 / Chapter 2.11.1 --- DNaseI treatment --- p.44 / Chapter 2.11.2 --- First strand synthesis --- p.44 / Chapter 2.11.3 --- Primer design and verification --- p.45 / Chapter 2.11.4 --- Real-time PCR --- p.45 / Chapter 2.11.5 --- Statistical analysis --- p.45 / Chapter Chapter 3 --- Results / Chapter 3.1 --- Experimental animals --- p.50 / Chapter 3.2 --- Total RNA extraction --- p.50 / Chapter 3.3 --- Library construction --- p.50 / Chapter 3.4 --- PCR screening of inserted sequences --- p.50 / Chapter 3.5 --- RNA arbitrarily primed polymerase chain reaction (RAP-PCR) --- p.51 / Chapter 3.6 --- Dot blot hybridization --- p.51 / Chapter 3.7 --- Sequencing and BLAST search --- p.52 / Chapter 3.8 --- Northern blot analysis --- p.53 / Chapter 3.8.1 --- Housekeeping gene - elongation factor la --- p.53 / Chapter 3.8.2 --- Insulin-like growth factor binding protein (IGFBP) --- p.54 / Chapter 3.8.3 --- Arrestin --- p.54 / Chapter 3.8.4 --- Opsin --- p.54 / Chapter 3.9 --- Real-time RT-PCR --- p.55 / Chapter 3.9.1 --- β-Actin --- p.55 / Chapter 3.9.2 --- Farnesoic acid O-methyltranferase (FAMeT) --- p.56 / Chapter Chapter 4 --- Discussion / Chapter 4.1 --- Application of RAP-PCR and dot blot analysis in identifying differential expressed genes --- p.79 / Chapter 4.1.1 --- Abundant genes --- p.79 / Chapter 4.1.2 --- Appearance of 16S rRNA in eyestalk cDNA library of M. ensis --- p.80 / Chapter 4.1.3 --- False positive --- p.80 / Chapter 4.2 --- Investigation on common housekeeping genes --- p.81 / Chapter 4.3 --- Potential functions of identified differential expressed genes in reproduction of shrimp --- p.82 / Chapter 4.3.1 --- Arrestin --- p.84 / Chapter 4.3.2 --- Opsin --- p.86 / Chapter 4.3.3 --- Insulin-like growth factor binding protein (IGFBP) --- p.88 / Chapter 4.3.4 --- β-Actin --- p.89 / Chapter 4.4 --- Investigation for farnesoic acid O-methyltransferase (FAMeT) in shrimp --- p.89 / Chapter Chapter 5 --- General Conclusion --- p.91 / References --- p.95 / Appendix --- p.116

published_or_final_version / abstract / toc / Zoology / Master / Master of Philosophy

published_or_final_version / Zoology / Master / Master of Philosophy

Made available in DSpace on 2014-06-11T19:35:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-07-27Bitstream added on 2014-06-13T21:07:58Z : No. of bitstreams: 1 simoes_sm_dr_botib.pdf: 2480639 bytes, checksum: 742a2cba98aff1b821711418308e9eb4 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A finalidade do presente estudo foi analisar a estrutura da comunidade dos camarões marinhos (Penaeidea e Caridea) encontrados no Complexo Baía-Estuário de Santos e São Vicente enfocando a composição de espécies, os índices de diversidade, equidade, similaridade e, a distribuição ecológica dos indivíduos em relação aos fatores ambientais. Os fatores bióticos e abióticos (temperatura, salinidade, granulometria e matéria orgânica) foram coletados durante o período de maio/2008 a abril/2010, mensalmente, em 4 pontos no estuário e 4 pontos na baía. As coletas foram realizadas com um barco camaroneiro equipado com uma rede do tipo otter-trawl. Foi estimada a diversidade (H’) através do índice de Shannon-Wiener, a equidade (J’) e utilizou-se a análise de cluster para verificar a similaridade das espécies entre as estações do ano e ponto amostral. A análise de correlação canônica foi empregada com intuito de observar a relação entre as espécies e entre os indivíduos com os fatores ambientais analisados. No estuário, os peneídeos tiveram representantes somente da família Penaeidae, sendo elas: Litopenaeus schmitti, Rimapenaeus constrictus, Farfantepenaeus paulensis e F. brasiliensis. Os camarões carídeos foram pertencentes à família Palaemonidae (Leander paulensis e Macrobrachium acanthurus), a família Alpheidae (Alpheus intrinsecus, A. pontederiae, A. cf. armillatus, A. cf. lobidens, Synalpheus apioceros e Athanas nitescens) e a família Hippolytidae representada por Lysmata rauli. Na baía, os peneídeos foram representados por espécies da família Penaeidae (Xiphopenaeus kroyeri, L. schmitti, R. constrictus, F. paulensis, F. brasiliensis, Artemesia longinaris), da família Solenoceridae (Pleoticus muelleri) e da família Sicyoniidae... / Not available

Orientador: Rogério Caetano da Costa / Banca: Alexandre Oliveira de Almeida / Banca: Antonio Leão Castilho / Banca: Maria Lucia Negreiros-Fransozo / Banca: Luís Medina Mantelatto / Resumo: A finalidade do presente estudo foi analisar a estrutura da comunidade dos camarões marinhos (Penaeidea e Caridea) encontrados no Complexo Baía-Estuário de Santos e São Vicente enfocando a composição de espécies, os índices de diversidade, equidade, similaridade e, a distribuição ecológica dos indivíduos em relação aos fatores ambientais. Os fatores bióticos e abióticos (temperatura, salinidade, granulometria e matéria orgânica) foram coletados durante o período de maio/2008 a abril/2010, mensalmente, em 4 pontos no estuário e 4 pontos na baía. As coletas foram realizadas com um barco camaroneiro equipado com uma rede do tipo otter-trawl. Foi estimada a diversidade (H') através do índice de Shannon-Wiener, a equidade (J') e utilizou-se a análise de cluster para verificar a similaridade das espécies entre as estações do ano e ponto amostral. A análise de correlação canônica foi empregada com intuito de observar a relação entre as espécies e entre os indivíduos com os fatores ambientais analisados. No estuário, os peneídeos tiveram representantes somente da família Penaeidae, sendo elas: Litopenaeus schmitti, Rimapenaeus constrictus, Farfantepenaeus paulensis e F. brasiliensis. Os camarões carídeos foram pertencentes à família Palaemonidae (Leander paulensis e Macrobrachium acanthurus), a família Alpheidae (Alpheus intrinsecus, A. pontederiae, A. cf. armillatus, A. cf. lobidens, Synalpheus apioceros e Athanas nitescens) e a família Hippolytidae representada por Lysmata rauli. Na baía, os peneídeos foram representados por espécies da família Penaeidae (Xiphopenaeus kroyeri, L. schmitti, R. constrictus, F. paulensis, F. brasiliensis, Artemesia longinaris), da família Solenoceridae (Pleoticus muelleri) e da família Sicyoniidae... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Not available / Doutor

published_or_final_version / abstract / Biological Sciences / Doctoral / Doctor of Philosophy

Sex-specific nutritional requirements for crude protein and fat were demonstrated in cultured (F1) Penaeus stylirostris brood stock. Female shrimp required diets which had higher protein (32 versus 27%), lower fat (2.5 versus 3.9%), higher protein/calorie ratios (8.5 versus 6.8% protein/kcal/g), and much higher protein/fat ratios (15.4 versus 7.8% protein/% fat) than males. These studies have also demonstrated a nutritional demand corresponding to the onset of ovarian maturation, a phenomenon which was explained as a reduction in growth rates at the attainment of 30 to 35 g in shrimp fed deficient diets. Both the quality and the quantity of dietary fat were shown to affect the growth of P. stylirostris brood stock. Male growth was positively correlated with the quantity of eicosapentaenoic acid (20:5 ω3) in the diets. The females were not affected by the types of fatty acids in the fat; they were influenced more by the quantity of fat, i.e., as the fat level of the diet increased, the growth decreased. Cold extrusion feed (CEF) diets supplemented with squid, and diets which included squid as one of the ingredients in the formulation, were found to stimulate better growth in both male and female brood stock as compared to CEF diets of equal protein and fat content without squid. The protein/fat ratio, as well as the content of polyunsaturated fatty acids (PUFA), were suggested to be responsible for the improved growth. Comparisons were made between the quality of spawns from wild P. stylirostris matured in captivity (P1) and F1 shrimp. Protein levels of the eggs did not correlate with either the number of eggs spawned or the eclosion rate. The number of the eggs spawned was correlated positively with the levels of eicosaenoic acid (20:1 ω9) in both P1 and F1 eggs, and correlated negatively with linoleic acid (18:2 ω6) in P1 eggs only. Spawning times were reported to occur later in the evening as summer approached. A significant, negative correlation was observed between the elapsed time from copulation, i.e., collection of fertilized shrimp, to spawning and eclosion rate. Also, a significant positive correlation was observed between the number of spawns which contained eggs which did not hatch, and the elapsed time from copulation to spawning. The number of eggs spawned and the eclosion rate were found to be higher in P1 shrimp as compared to F1 shrimp. Also, first breeding season spawners (FBS) had better quality spawns than second breeding season (SBS) spawners, i.e., more eggs with higher eclosion rates. A general reduction in the quality of the spawns was therefore implicated as a result of the culture conditions. Multiple spawning behavior was observed and there appeared to be no qualitative or quantitative difference between spawns. Tank size and shape were demonstrated to affect the onset of ovarian development and the transfer of the spermatophore. A minimum of three meters was thought to be required for the development of the ovaries and the successful transfer of the spermatophore.