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Robust Performance of Spider Viscid Silk During Prey CaptureAlicea-Serrano, Angela Maria 09 August 2022 (has links)
No description available.
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Protein Composition Correlates with the Mechanical Properties of Spider (<i>Argiope Trifasciata</i>) Dragline SilkMarhabaie, Mohammad 20 September 2013 (has links)
No description available.
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73 |
From Nano to Micro to Macro: Importance of Structure and Architecture in Spider Silk AdhesivesSahni, Vasav 24 July 2012 (has links)
No description available.
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The effect of certain light rays upon weighted and unweighted silk fabricsHaas, Golda Pearle. January 1933 (has links)
Call number: LD2668 .T4 1933 H305
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Characterization of silk proteins from African wild silkworm cocoons and application of fibroin matrices as biomaterialsMhuka, Vimbai 11 1900 (has links)
Challenges in treating injuries, together with an increased need for repair of damaged tissues and organs, have made regenerative medicine a major research area today. Biomaterials such as silk fibroin (SF) have proven to be excellent tissue scaffolds possessing properties essential in tissue engineering such as biocompatibility, biodegradability and exceptional mechanical properties. SF nanofibres are especially attractive due to their large surface-to-volume ratio and high porosity which is beneficial in regenerative medicine. However, to design biomaterial scaffolds, chemical and physical properties of SF have to be sufficiently known. The thesis aims to contribute to knowledge by characterizing silk fibroin from the African wild silkworm species Gonometa rufobrunnae, Gonometa postica, Argema mimosae, Epiphora bahuniae and Anaphe panda. Moreover, the feasibility of producing nanofibrous biomaterial scaffolds from these fibroins is explored.
The chemical composition of degummed fibres was investigated using Capillary electrophoresis whilst Infrared (IR) and Raman spectroscopic techniques were utilized to determine structural characteristics of the fibroin. In addition, thermal behaviour and mechanical properties of the fibroins were also investigated. Nanofibres were fabricated via electrospinning. The effects of solution concentration, voltage, polymer flow rate and tip to collector distance were studied to give optimum electrospinning conditions. IR spectroscopy was also utilized to observe the conformational structure of the degummed and electrospun fibres whilst scanning electron microscopy (SEM) provided information on the size and morphology of the fibres. The use of the nanofibres as biomaterials was evaluated using cytotoxicity tests.
Results showed that glycine, alanine and serine constituted over 70% of the amino acid composition of all the fibroins. Gonometa fibroin had more glycine than alanine whilst the opposite was true for Argema mimosae, Epiphora bahuniae and Anaphe panda fibroin. The abundance of basic amino acids in Gonometa rufobrunnae, Gonometa postica, Argema mimosae and Epiphora bahuniae fibroin makes them prime candidates for cell and tissue culture. The amino acid composition of the fibroins influenced secondary structure as the β-sheet structure. Anaphe panda, Argema mimosae and Epiphora bahuniae silks was made up of mostly alanine-alanine (Ala-Ala)n polypeptides whilst Gonometa fibroin had an interesting mixture of both glycine-alanine (Gly-Ala)n and (Ala-Ala)n units. The unique structures impacted the mechanical and thermal properties of the fibroins.
Production of Gonometa nanofibres was mainly dependent on fibroin solution concentration. A minimum of 27 % w/v was needed to produce defect free nanofibres. Diameters of the electrospun fibres produced ranged from 300 to 2500 nm. IR spectroscopy data highlighted that the β-sheet conformation of degummed fibroin was degraded during the formation of the nanofibres rendering them water soluble. It was however possible to regenerate the β-sheet structure in the nanofibres by exposing them to various solvents. Cytotoxicity tests using Sulforhodamine B (SRB) assay demonstrated that the nanofibres were not toxic to cells, a major prerequisite for use as a biomaterial.
This thesis successfully provides useful data in an area that has been minimally explored. Results suggest that SF from African silkworm species offers diversity in properties and are therefore attractive for use as biomaterials, especially in cell and tissue engineering. As far as we could determine, we are the first to extend the use of fibroin from African silk species by producing Gonometa SF nanofibres that are of potential use as biomaterial scaffolds. / Chemistry / D. Phil. (Chemisty)
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The ontology of the Venetian halo in its Italian contextMartin, Susan Morag January 2015 (has links)
This thesis aims to reposition the halo’s status within an artwork through arguing a reassessment of its activity 'as a sign' rather than acceptance of its passivity. This active state is further explored and expanded by a heuristic application of semiotic theory to interrogate its fluctuation between sign/non-sign and its oscillation between a seemingly real status and behaviour juxtaposed with its very consciously artificial “manifestation”. A variety of halo shapes are considered, together with texture contained in and on its surface, and this has revealed the Venetian and Venetan artistic innovation of “glass” and “silk” haloes, through artists’ utilisation of contemporaneous industrial practices and their application to halo appearance. Additionally, extant architectural vocabulary is translated and reformulated into internal halo motifs by Venetian and Venetan artists, further enhancing the halo’s somatic characteristics, contextualized by examination of halo representation in various media in Florence, Rome and Siena, and a consideration of haloes within other, mainly Italian, centres. Additionally, the fugitive and transient qualities of the nimbus are noted, with its mimesis of the dying corporeal body in its fading insubstantiality, a further factor in its inexorably reductive form as increasing realism in art challenges its ontological traits. Textual characters contained within the halo body are also examined in their many forms and languages and their contribution to an intertextual function espoused by the ideologeme. An adjunct to this function is the halo’s propagandist role presented by artists. It will be demonstrated how all these different strands of interpretation are imbricated in the changing theological, political and societal landscape, encapsulated within the halo.
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Fabrication and Characterization of Recombinant Silk-elastinlike Protein Fibers for Tissue Engineering ApplicationsQiu, Weiguo January 2011 (has links)
The integration of functional and structural properties makes genetically engineered proteins appealing in tissue engineering. Silk-elastinlike proteins (SELPs), containing tandemly repeated polypeptide sequence derived from natural silk and elastin, are recently under active study due to the interesting structure. The biological, chemical, physical properties of SELPs have been extensively investigated for their possible applications in drug/gene delivery, surgical tissue sealing and spine repair surgery. However, the mechanical aspect has rarely been looked into. Moreover, many other biomaterials have been fabricated into fibers in micrometer and nanometer scale to build extracellular matrix-mimic scaffolds for tissue regeneration, but many have one or mixed defects such as: poor strength, mild toxicity or immune repulsion etc. The SELP fibers, with the intrinsic primary structures, have novel mechanical properties that can make them defects-minimized scaffolds in tissue engineering.In this study, one SELP (SELP-47K) was fabricated into microfibers and nanofibers by the techniques of wet-spinning and electrospinning. Microfibers of meters long were formed and collected from a methanol coagulation bath, and later were crosslinked by glutaraldehyde (GTA) vapor. The resultant microfibers displayed higher tensile strength up to 20 MPa and higher deformability as high as 700% when tested in hydrated state. Electrospinnig of SELP-47K in formic acid and water resulted in rod-like and ribbon-like nanofibrous scaffolds correspondingly. Both chemical (methanol and/or GTA) and physical (autoclaving) crosslinking methods were utilized to stabilize the scaffolds. The chemical crosslinked hydrated scaffolds exhibit elastic moduli of 3.4-13.2 MPa, ultimate tensile strength of 5.7-13.5 MPa, and deformability of 100-130%, closely matching or exceeding the native aortic elastin; while the autoclaved one had lower numbers: 1.0 MPa elastic modulus, 0.3 MPa ultimate strength and 29% deformation. However, the resilience was all above 80%, beyond the aortic elastin, which is 77%. Additionally, Fourier transform infrared spectra showed clear secondary structure transition after crosslinking, explaining the phenomenon of scaffold water-insolubility from structural perspective and showed a direct relationship with the mechanical performance. Furthermore, the in vitro biocompatibility of SELP-47K nanofibrous scaffolds were verified through the culture of NIH 3T3 mouse embryonic fibroblast cells.
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The smoothened gene in Drosophila and vertebrate developmentQuirk, Jeremy Paul January 1999 (has links)
No description available.
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Silk Fibroin-Based Scaffolds for Tissue Engineering ApplicationsMcCool, Jennifer 27 July 2011 (has links)
This study focused on the comparison of the electrospun silk scaffolds to the electrospun silk fibroin gel scaffolds. Moreover, this study examined the differences in cross-linking effects of genipin and methanol as well as solvents on the mechanical properties and cell compatibility of the scaffolds. Silk scaffolds were electrospun from an aqueous solution or 1,1,1,3,3-hexafluoro-2-propanol (HFIP) without genipin, immediately after 8 % (wt) genipin was added to the solution, and 18 hours after genipin blended with the solution. Uniaxial tensile testing determined that the silk scaffolds electrospun from water exhibit a higher modulus and peak stress than that of the silk scaffolds electrospun from HFIP. In vitro cell culture was conducted to determine the cell compatibility of the various silk fibroin-based scaffolds. 4'-6-Diamidino-2-phenylindole (DAPI) staining and histology suggest that genipin may enhance cell compatibility, and that neither ethanol nor methanol inhibit cell interactions.
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80 |
Characterization of silk proteins from African wild silkworm cocoons and application of fibroin matrices as biomaterialsMhuka, Vimbai 11 1900 (has links)
Challenges in treating injuries, together with an increased need for repair of damaged tissues and organs, have made regenerative medicine a major research area today. Biomaterials such as silk fibroin (SF) have proven to be excellent tissue scaffolds possessing properties essential in tissue engineering such as biocompatibility, biodegradability and exceptional mechanical properties. SF nanofibres are especially attractive due to their large surface-to-volume ratio and high porosity which is beneficial in regenerative medicine. However, to design biomaterial scaffolds, chemical and physical properties of SF have to be sufficiently known. The thesis aims to contribute to knowledge by characterizing silk fibroin from the African wild silkworm species Gonometa rufobrunnae, Gonometa postica, Argema mimosae, Epiphora bahuniae and Anaphe panda. Moreover, the feasibility of producing nanofibrous biomaterial scaffolds from these fibroins is explored.
The chemical composition of degummed fibres was investigated using Capillary electrophoresis whilst Infrared (IR) and Raman spectroscopic techniques were utilized to determine structural characteristics of the fibroin. In addition, thermal behaviour and mechanical properties of the fibroins were also investigated. Nanofibres were fabricated via electrospinning. The effects of solution concentration, voltage, polymer flow rate and tip to collector distance were studied to give optimum electrospinning conditions. IR spectroscopy was also utilized to observe the conformational structure of the degummed and electrospun fibres whilst scanning electron microscopy (SEM) provided information on the size and morphology of the fibres. The use of the nanofibres as biomaterials was evaluated using cytotoxicity tests.
Results showed that glycine, alanine and serine constituted over 70% of the amino acid composition of all the fibroins. Gonometa fibroin had more glycine than alanine whilst the opposite was true for Argema mimosae, Epiphora bahuniae and Anaphe panda fibroin. The abundance of basic amino acids in Gonometa rufobrunnae, Gonometa postica, Argema mimosae and Epiphora bahuniae fibroin makes them prime candidates for cell and tissue culture. The amino acid composition of the fibroins influenced secondary structure as the β-sheet structure. Anaphe panda, Argema mimosae and Epiphora bahuniae silks was made up of mostly alanine-alanine (Ala-Ala)n polypeptides whilst Gonometa fibroin had an interesting mixture of both glycine-alanine (Gly-Ala)n and (Ala-Ala)n units. The unique structures impacted the mechanical and thermal properties of the fibroins.
Production of Gonometa nanofibres was mainly dependent on fibroin solution concentration. A minimum of 27 % w/v was needed to produce defect free nanofibres. Diameters of the electrospun fibres produced ranged from 300 to 2500 nm. IR spectroscopy data highlighted that the β-sheet conformation of degummed fibroin was degraded during the formation of the nanofibres rendering them water soluble. It was however possible to regenerate the β-sheet structure in the nanofibres by exposing them to various solvents. Cytotoxicity tests using Sulforhodamine B (SRB) assay demonstrated that the nanofibres were not toxic to cells, a major prerequisite for use as a biomaterial.
This thesis successfully provides useful data in an area that has been minimally explored. Results suggest that SF from African silkworm species offers diversity in properties and are therefore attractive for use as biomaterials, especially in cell and tissue engineering. As far as we could determine, we are the first to extend the use of fibroin from African silk species by producing Gonometa SF nanofibres that are of potential use as biomaterial scaffolds. / Chemistry / D. Phil. (Chemisty)
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