O referencial da Teoria da Localização embasa o modelo matemático de otimização dinâmico desenvolvido a fim de se determinar os melhores locais dos frigoríficos-abatedouros exportadores no Brasil visando à minimização dos custos associados ao transporte, bem como os custos associados à instalação de novas unidades industriais de abate, resultando, assim, em aumento de competitividade para a cadeia. Este trabalho visa responder a três questões: (i) localizações das unidades industriais de abate; (ii) tamanho e número ótimos de cada unidade industrial de abate e (iii) fluxos mensais da matéria-prima necessários para atender a demanda dos frigoríficos-abatedouros exportadores, bem como os fluxos ofertados mensalmente por eles a fim de se satisfazer a demanda dos países importadores da carne bovina brasileira. Desse modo, foram simulados quatro cenários alternativos. Os dois primeiros disseram respeito sobre a determinação da localização dos frigoríficos-abatedouros exportadores e respectivos fluxos de produtos num contexto ideal, ou seja, o modelo determinou onde os mesmos deveriam ser instalados. Enquanto o primeiro cenário considerou na modelagem os dez principais estados exportadores da carne bovina desossada congelada brasileira, o segundo considerou os seis principais estados, excluindo, assim, os estados que fazem parte da Amazônia Legal. No primeiro cenário, dos dez estados considerados, em cinco deveriam ser instalados ao menos um frigoríficoabatedouro exportador, enquanto no segundo cenário dos seis estados considerados em três deveriam ser instalados ao menos um frigorífico-abatedouro exportador. Ambos os cenários indicaram São Paulo como estado candidato à instalação de maior número de frigoríficosabatedouros exportadores. Além disso, em ambos os cenários a decomposição dos custos logísticos revelou que a maior parte do custo total mínimo obtido nos processamentos dos modelos decorreu da instalação dos frigoríficos-abatedouros exportadores,76,3% do custo total mínimo obtido no cenário 1 e 79,4% do custo total mínimo obtido no cenário 2, seguidos pelo custo de transporte da carne bovina aos mercados externos (14,9% e 14,1%) e custo de transporte da carne bovina aos portos exportadores (5,7% e 3,6%). O custo de transporte da matéria-prima aos frigoríficos-abatedouros exportadores foi 1,9% e 1,2% do custo total mínimo obtido no cenário 1 e 2, respectivamente, ao passo que o custo de transporte da carne bovina aos mercados internos foi de 1,2% e 1,7% do respectivo custo total mínimo. O terceiro e quarto cenários disseram respeito sobre quais frigoríficos-abatedouros exportadores já instalados deveriam ser responsáveis pelo abastecimento dos mercados externos demandantes de carne bovina desossada congelada brasileira. Ambos consideraram na modelagem as localizações atuais dos frigoríficos abatedouros exportadores existentes nos seis principais estados exportadores do produto. No entanto, o último cenário refere-se a projeção de aumento de demanda externa futura por carne bovina. Nesses cenários, como não há custo de intalação dos frigoríficos-abatedouros exportadores a ser incorridos, a maior parcela dos custos logísticos decorreu da distribuição da carne bovina aos mercados externos. Os resultados do terceiro cenário revelaram que 12 frigoríficos-abatedouros exportadores seriam suficientes para atender plenamente os mercados externos, ao passo que o aumento da demanda externa futura exigirá 14 frigoríficos-abatedouros exportadores. / The theoretical framework of the Location Theory supports the dynamic optimization mathematical model developed in order to determine the potential sites for the installation of exporter slaughterhouses in Brazil, minimizing the costs associated with transportation and the costs associated with the installation of new slaughter industrial units, thereby increasing the competitiveness of the chain. The following three questions were addressed: (i) at what locations should slaughter industrial units be installed; (ii) what is the optimal-size and number of each slaughter industrial, and (iii) what are the flows of raw material required monthly to meet the exporter slaughterhouses demand and the monthly supplied amount by them in order to meet the importing countries demands? Thus, four alternative scenarios were simulated. The first two scenarios are related to determine the exporter slaughterhousess locations and the products flows in an ideal environment, i.e., the model determines where they should be installed. The difference between these scenarios is that the former considers in the modeling the Brazilian top ten exporter states of frozen boneless beef, while the second considers the six major states, excluding, thus, the states that belong to the Amazon region. In the first scenario, at least one exporter slaughterhouse should be installed in five out of ten states considered, while in the second scenario at least one exporter slaughterhouse should be installed in three out of six states considered. Both scenarios indicated that most of the exporter slaughterhouses should be installed in São Paulo. Moreover, in both scenarios the decomposition of logistics costs has shown that most of the minimum total cost obtained in the models processing was due to the installation of the exporter slaughterhouses, 76.3% of the minimum total cost obtained in the scenario 1 and 79, 4% of the minimum total cost obtained in the scenario 2, followed by the cost of transporting beef up to the foreign markets (14.9% and 14.1%) and by the cost of transporting beef up to the exporter ports (5.7% and 3 , 6%). The cost of transporting the raw material up to the exporter slaughterhouses was 1.9% and 1.2% of the minimum total cost obtained in scenario 1 and 2, respectively, while the cost of transporting beef up to the domestic markets was 1.2% and 1.7% of the minimum total cost, respectively. The third and fourth scenarios concerned about which exporter slaughterhouses already installed should be responsible for fulfilling the demands of the external markets for the Brazilian frozen boneless beef. Both scenarios considered in the modeling the actual locations of the existing exporter slaughterhouses in the six main exporter states of the product. However, the latter scenario considers an increase in the beef future demand of the external markets. Since there is no cost related to the installation of the exporter slaughterhouses to be incurred in these scenarios, the largest share of logistics costs was related to the distribution of beef up to the foreign markets. The results of the third scenario indicates that 12 exporter slaughterhouses would be enough to fully meet the demands of the foreign markets for frozen boneless beef, while the increase in the external demands will require 14 exporter slaughterhouses.

O referencial da Teoria da Localização embasa o modelo matemático de otimização dinâmico desenvolvido a fim de se determinar os melhores locais dos frigoríficos-abatedouros exportadores no Brasil visando à minimização dos custos associados ao transporte, bem como os custos associados à instalação de novas unidades industriais de abate, resultando, assim, em aumento de competitividade para a cadeia. Este trabalho visa responder a três questões: (i) localizações das unidades industriais de abate; (ii) tamanho e número ótimos de cada unidade industrial de abate e (iii) fluxos mensais da matéria-prima necessários para atender a demanda dos frigoríficos-abatedouros exportadores, bem como os fluxos ofertados mensalmente por eles a fim de se satisfazer a demanda dos países importadores da carne bovina brasileira. Desse modo, foram simulados quatro cenários alternativos. Os dois primeiros disseram respeito sobre a determinação da localização dos frigoríficos-abatedouros exportadores e respectivos fluxos de produtos num contexto ideal, ou seja, o modelo determinou onde os mesmos deveriam ser instalados. Enquanto o primeiro cenário considerou na modelagem os dez principais estados exportadores da carne bovina desossada congelada brasileira, o segundo considerou os seis principais estados, excluindo, assim, os estados que fazem parte da Amazônia Legal. No primeiro cenário, dos dez estados considerados, em cinco deveriam ser instalados ao menos um frigoríficoabatedouro exportador, enquanto no segundo cenário dos seis estados considerados em três deveriam ser instalados ao menos um frigorífico-abatedouro exportador. Ambos os cenários indicaram São Paulo como estado candidato à instalação de maior número de frigoríficosabatedouros exportadores. Além disso, em ambos os cenários a decomposição dos custos logísticos revelou que a maior parte do custo total mínimo obtido nos processamentos dos modelos decorreu da instalação dos frigoríficos-abatedouros exportadores,76,3% do custo total mínimo obtido no cenário 1 e 79,4% do custo total mínimo obtido no cenário 2, seguidos pelo custo de transporte da carne bovina aos mercados externos (14,9% e 14,1%) e custo de transporte da carne bovina aos portos exportadores (5,7% e 3,6%). O custo de transporte da matéria-prima aos frigoríficos-abatedouros exportadores foi 1,9% e 1,2% do custo total mínimo obtido no cenário 1 e 2, respectivamente, ao passo que o custo de transporte da carne bovina aos mercados internos foi de 1,2% e 1,7% do respectivo custo total mínimo. O terceiro e quarto cenários disseram respeito sobre quais frigoríficos-abatedouros exportadores já instalados deveriam ser responsáveis pelo abastecimento dos mercados externos demandantes de carne bovina desossada congelada brasileira. Ambos consideraram na modelagem as localizações atuais dos frigoríficos abatedouros exportadores existentes nos seis principais estados exportadores do produto. No entanto, o último cenário refere-se a projeção de aumento de demanda externa futura por carne bovina. Nesses cenários, como não há custo de intalação dos frigoríficos-abatedouros exportadores a ser incorridos, a maior parcela dos custos logísticos decorreu da distribuição da carne bovina aos mercados externos. Os resultados do terceiro cenário revelaram que 12 frigoríficos-abatedouros exportadores seriam suficientes para atender plenamente os mercados externos, ao passo que o aumento da demanda externa futura exigirá 14 frigoríficos-abatedouros exportadores. / The theoretical framework of the Location Theory supports the dynamic optimization mathematical model developed in order to determine the potential sites for the installation of exporter slaughterhouses in Brazil, minimizing the costs associated with transportation and the costs associated with the installation of new slaughter industrial units, thereby increasing the competitiveness of the chain. The following three questions were addressed: (i) at what locations should slaughter industrial units be installed; (ii) what is the optimal-size and number of each slaughter industrial, and (iii) what are the flows of raw material required monthly to meet the exporter slaughterhouses demand and the monthly supplied amount by them in order to meet the importing countries demands? Thus, four alternative scenarios were simulated. The first two scenarios are related to determine the exporter slaughterhousess locations and the products flows in an ideal environment, i.e., the model determines where they should be installed. The difference between these scenarios is that the former considers in the modeling the Brazilian top ten exporter states of frozen boneless beef, while the second considers the six major states, excluding, thus, the states that belong to the Amazon region. In the first scenario, at least one exporter slaughterhouse should be installed in five out of ten states considered, while in the second scenario at least one exporter slaughterhouse should be installed in three out of six states considered. Both scenarios indicated that most of the exporter slaughterhouses should be installed in São Paulo. Moreover, in both scenarios the decomposition of logistics costs has shown that most of the minimum total cost obtained in the models processing was due to the installation of the exporter slaughterhouses, 76.3% of the minimum total cost obtained in the scenario 1 and 79, 4% of the minimum total cost obtained in the scenario 2, followed by the cost of transporting beef up to the foreign markets (14.9% and 14.1%) and by the cost of transporting beef up to the exporter ports (5.7% and 3 , 6%). The cost of transporting the raw material up to the exporter slaughterhouses was 1.9% and 1.2% of the minimum total cost obtained in scenario 1 and 2, respectively, while the cost of transporting beef up to the domestic markets was 1.2% and 1.7% of the minimum total cost, respectively. The third and fourth scenarios concerned about which exporter slaughterhouses already installed should be responsible for fulfilling the demands of the external markets for the Brazilian frozen boneless beef. Both scenarios considered in the modeling the actual locations of the existing exporter slaughterhouses in the six main exporter states of the product. However, the latter scenario considers an increase in the beef future demand of the external markets. Since there is no cost related to the installation of the exporter slaughterhouses to be incurred in these scenarios, the largest share of logistics costs was related to the distribution of beef up to the foreign markets. The results of the third scenario indicates that 12 exporter slaughterhouses would be enough to fully meet the demands of the foreign markets for frozen boneless beef, while the increase in the external demands will require 14 exporter slaughterhouses.

Campylobacter est la principale cause de gastro-entérite bactérienne d’origine alimentaire à travers le monde. Chez les consommateurs, les campylobactérioses d’origine alimentaire sont en grande majorité dues au contact et à la consommation de produits de volaille, le poulet à griller étant particulièrement mis en cause. La contamination de la carcasse se fait souvent lors de l’abattage des oiseaux. Bien que plusieurs données concernant la distribution de Campylobacter à l’abattoir soient disponibles dans certains pays, aucune étude récente visant à décrire la présence et la distribution de ce pathogène, tout au long de la chaîne d’abattage, n'a été réalisée au niveau des établissements d'abattage canadiens. Notre hypothèse était que l’on pouvait identifier des étapes clés d’intervention pour contrôler la contamination par Campylobacter sur les carcasses de volaille à l’abattoir en étudiant la présence de ce pathogène sur les produits de viande et dans l’environnement de production. Il y avait deux objectifs principaux dans cette étude. Premièrement, nous voulions décrire la distribution de Campylobacter lors des différentes étapes de production dans deux établissements de transformation québécois. Deuxièmement, nous voulions déterminer si les moyens de gestion du risque mis en place au moment de l’étude étaient suffisants pour prévenir la contamination du produit de viande destiné au consommateur. Pour répondre à ces objectifs, un oiseau par lot d’élevage a été échantillonné par rinçât de carcasse pour chaque étape suivante de la transformation (n=4) : avant l'abattage, tout juste après la saignée, au transfert entre les chaînes d'abattage et d'éviscération, après l'éviscération et après l’étape du refroidissement dans deux abattoirs québécois. Cette procédure fut répétée pour un total de 379 échantillons de rinçâts de carcasses de poulets de chair qui ont été collectés à l’occasion de multiples visites à l’abattoir et ce, de février à juillet 2017. Un total de 217 échantillons environnementaux pouvant être impliqués dans la contamination croisée des oiseaux ont aussi été récupéré pendant les diverses visites. Les échantillons ont été dilués dans de l'eau peptonée et une identification de Campylobacter par PCR a été faite à l’aide d’amorces spécifiques au gène codant pour l’ARN ribosomal 16S. Les résultats obtenus pour la période étudiée indiquent que la positivité des carcasses de poulets de chair à Campylobacter est significativement plus élevée pour les échantillons effectués l’été comparé au printemps. En revanche, la présence de la bactérie dans l’environnement des abattoirs étudiés apparaît plus élevée durant l’hiver. Puisque la présence de la bactérie sur les carcasses de poulets a diminué tout au long de la chaîne de production, qu’aucune carcasse positive n’a été retrouvée après un refroidissement à l’air et que la positivité des carcasses suite au refroidissement à l’eau était aussi très basse, nos résultats suggèrent, malgré certains enjeux associés à la sensibilité de la méthode d’identification des échantillons positifs, que les mesures actuelles de gestion du risque sont efficaces pour contrôler Campylobacter dans les deux abattoirs québécois suivis. / Campylobacter is responsible of the highest number of bacterial gastroenteritis worldwide. Most diseases in humans attributed to meat can be associated to consumption of or contact with poultry derived products; broiler chicken being involved in the majority of cases. The contamination of carcasses often occurs during the slaughter process. While many studies from various countries reported the distribution of Campylobacter among various critical steps of the slaughter process, none has been published, to our knowledge, in Canada regarding the presence and distribution of this bacterium within the abattoir. Our hypothesis was that it would be possible to identify key steps to control the contamination of carcasses by this bacterium by studying the presence and distribution of Campylobacter on carcasses and within the environment during the slaughter process. This study had two objectives. The first objective was to describe the distribution of Campylobacter within two selected slaughterhouses in Quebec in order to understand which processing step(s) play(s) a critical role in carcasses contamination. In the second objective, we aimed to verify if actual management procedures applied in these abattoirs were efficient in preventing consumer’s exposition. To meet these goals, four birds by production lot, one at each of the following steps (after bleeding, at transfer between killing and evisceration chain, after evisceration and after the cooling process) were sampled for a total of 379 birds from February 2017 to July 2017 in two slaughterhouses located in the province of Quebec. Furthermore, 217 environmental samples were collected during these visits in various sites possibly in contact with birds. Samples were suspended in peptone water and submitted to a PCR assay, using a specific 16S ribosomal probe, for detection of Campylobacter. Overall, for the year of the study, we observed a significantly higher number of positive carcasses in summer compared to spring, while the environmental samples were more often positive in winter compared with summer. Furthermore, our results indicated that the number of positive carcasses decreased over the various processing steps, being either negative (air chilling) or low (water chilling) after the cooling process. Although we experienced some issues associated with the sensitivity of the procedure we used in this study to recover Campylobacter, taken together, these results suggest that the actual management procedures of Campylobacter in studied slaughterhouses are efficient.

Made available in DSpace on 2015-03-26T13:52:01Z (GMT). No. of bitstreams: 1 texto completo.pdf: 582896 bytes, checksum: f0af95b88bc9afacbb9f1a952768a98e (MD5) Previous issue date: 2008-10-03 / Conselho Nacional de Desenvolvimento Científico e Tecnológico / Shiga-toxigenic Escherichia coli, STEC, has emerged as a pathogen that can cause food-borne infections and severe and potentially fatal diseases such as hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Most HC and HUS outbreaks have been attributed to strains of entero-hemorrhagic serotype O157:H7, but there is growing interest on the risk to human health associated with the non-O157 STEC serotypes carried by beef products contaminated by ruminant feces. This study aimed to determine the occurrence of STEC in a cattle slaughterhouse located in the State of Minas Gerais. Sampling was carried out in two lots; Lot A with 75 bovines and Lot B with 51 animals in three stages of the slaughtering line: removal of skin after bleeding, carcass after sawing, and feces during evisceration. Samples were collected from ten animals of each lot. The samples were enriched in modified EC broth (mEC) and modified EC added with novobiocin (mECn) followed by isolation of typical colonies on Sorbitol MacConkey Medium. Two hundred and forty-one bacterial isolates obtained from the two lots, were identified as E. coli. Of these, 104 were derived from the skin, 93 from fecal material and 44 from carcasses. Polymerase chain reaction detected the stx gene in 18 isolates of E. coli. These isolates were obtained from six animals: four were of fecal origin and come from lot A; two were from carcasses from lot B. Enrichment in mECn resulted in isolates originated from one animal, whereas in mEC, the isolates were originated from five bovines. Isolates stx positive were analyzed for the hlyA gene encoding enterohemolysin. Four hlyA positive isolates originated from two bovines, one from lot A and another from lot B. Two displayed the entero- hemolytic phenotype and were isolated from the carcass. Ten of the 18 potentially STEC isolates showed cytotoxic effect on Vero cells. Eight isolates from three different animals had the stx PCR amplicons sequenced. The translated sequences obtained from stx of three isolates from the carcass and of one isolate from feces corresponded to Stx2, showing 98% to 99% identity, with references at the GeneBank (NCBI). The sequences inferred from four amplicons obtained from isolates of faecal origin corresponded to Stx1, with 94% to 96% identity. The presence of STEC in the feces and carcasses of cattle at a slaughterhouse in the state of Minas Gerais emphasizes the importance of establishing a HACCP system and the need for implementation, evaluation, and validation of control measures to minimize the risk of cross-contamination during and after slaughter. / Escherichia coli Shiga-toxigênica, STEC, tem emergido como patógeno que pode causar infecções de origem alimentar e doenças severas e potencialmente fatais, como Colite Hemorrágica (CH) e Síndrome Urêmico Hemolítica (SUH). A maioria dos surtos de CH e SUH tem sido atribuída a estirpes do sorotipo entero-hemorrágico O157:H7, entretanto existe interesse crescente sobre o risco à saúde humana associado com os sorotipos STEC não-O157 veiculados em produtos de carne bovina, contaminados por fezes de ruminantes. Este trabalho teve como objetivos determinar a ocorrência de STEC em bovinos em matadouro frigorífico, localizado no Estado de Minas Gerais. A amostragem foi realizada em dois lotes, lote A com 75 bovinos e lote B com 51 bovinos, em três etapas da linha de abate: pele, após a sangria; carcaça, após a serragem; e fezes, durante a evisceração. Dez animais de cada lote foram amostrados. As amostras foram enriquecidas em caldo EC modificado, mEC e EC modificado acrescido de Novobiocina, mECn, seguindo-se o isolamento de colônias típicas em meio MacConkey sorbitol. Duzentos e quarenta e um isolados bacterianos obtidos nos dois lotes foram identificados como E. coli. Desses, 104 foram provenientes da pele, 93 do material fecal e 44 das carcaças. Por reação em cadeia de polimerase, foi detectado o gene stx em 18 isolados de E. coli. Esses isolados foram provenientes de seis bovinos, sendo quatro do lote A (origem fecal) e dois do lote B (carcaça). O enriquecimento em meio mECn resultou em isolados originados de um bovino, enquanto no mEC os isolados se originaram de cinco bovinos. Os isolados stx positivos foram posteriormente analisados para o gene hlyA, codificador de entero-hemolisina. Quatro isolados, positivos para o gene hlyA, foram provenientes de dois bovinos, um do lote A (origem fecal) e outro do lote B (carcaça). Desses, dois isolados da carcaça apresentaram fenótipo entero-hemolítico. Dez dos 18 isolados potencialmente STEC demonstraram ter efeito citotóxico em células Vero. Oito isolados, provenientes de três bovinos distintos, tiveram os amplicons stx seqüenciados. As seqüências traduzidas de três isolados da carcaça e de um das fezes corresponderam a Stx2, com 98% a 99% de identidade, com referências no GeneBank (NCBI). As seqüências inferidas de quatro amplicons, de origem fecal, corresponderam, com 94% a 96% de identidade, a Stx1. A presença de STEC nas fezes e carcaça de bovinos em matadouro frigorífico no Estado de Minas Gerais enfatiza a importância de implementação do sistema HACCP, e a necessidade de implementar, avaliar e validar medidas de controle que minimizem os riscos de contaminação cruzada durante o processo de abate.

Made available in DSpace on 2016-04-04T18:21:57Z (GMT). No. of bitstreams: 1 Douglas Murilha-1.pdf: 14097136 bytes, checksum: de6509aa6792a81ed834e6fa52dc439d (MD5) Previous issue date: 2011-02-15 / This research aims to study the theories and concepts hygienists present on the body of doctors and engineers, from the early nineteenth century until the mid-twentieth century, checking the impact thereof on the construction of public buildings - markets and slaughterhouses . We also intend to investigate an analytical perspective that focuses on the constructive-typological conceptions of slaughterhouses and public markets of these cities, which are the objects of investigation of research both in Europe and Brazil. From the second half of the nineteenth century, the construction of slaughterhouses and markets began to be administered by the municipalities of S?o Paulo Province. These buildings were linked to food production and movement of goods. Generally, they were built in locations away from the urban center at the time, so as to cater to the principles hygienists. The epidemics that afflict European cities in Brazil were also concerned with the arrival of cholera by ship from Europe in the 1850s and led the Imperial Government passed obliging local councils to adopt their positions on health determinations, provided the imperial legislation of 1828, which are based on the theory that hygienist largely attributed to the "poison" in this environment the cause of several epidemics. Since then, the political hygienist who largely had their bases in the miasma theory, began to explain the control of government in the marketing of foodstuffs in the cities. Municipalities are now responsible for the construction and administration of abattoirs and markets, which, thereafter, they became public, no longer private. Many of these buildings built in cities in the interior have been demolished, and the Municipal Slaughterhouse of Campinas, designed by renowned architect-engineer Francisco de Paula Ramos de Azevedo. The slaughterhouse of Vila Mariana in Sao Paulo with Alberto Kuhlmann design, opened in 1887, was preserved and is now a cultural center. Still, it was preserved in Sao Paulo Municipal Market of Santo Amaro, opened in 1897, built in brick masonry, where it supplies the capital of timber, grain and other goods produced in the region or received from nearby cities like Itapecerica and Embu. Indeed, part of this heritage today tumbled by CONDEPHAAT Sao Paulo is linked to the production office of architect-engineer Francisco de Paula Ramos de Azevedo. This is the case Paulistano Municipal Market, opened in 1933, built in neoclassical style. Another example is the Municipal Market of Campinas, opened in 1908, also designed by Ramos de Azevedo, and this style neomourisco. This paper presents a set of case studies of public markets and slaughterhouses still exist in some cities in the interior, which are of significant historical value and still unprotected by the municipal bodies. / A presente pesquisa tem como objetivo o estudo das teorias e as concep??es higienistas presentes junto ao corpo de m?dicos e engenheiros, a partir do in?cio do s?culo XIX at? meados do s?culo XX, verificando a repercuss?o das mesmas na constru??o de edif?cios p?blicos mercados e matadouros. Pretende-se tamb?m investigar uma perspectiva de an?lise que foca as concep??es tipol?gicas-construtivas dos matadouros e mercados p?blicos destas cidades, sendo estes os objetos de investiga??o da pesquisa, tanto na Europa como no Brasil. A partir da segunda metade do s?culo XIX, a constru??o de matadouros e mercados passaram a ser administrados pelas municipalidades da Prov?ncia Paulista. Estes edif?cios estavam ligados ? produ??o aliment?cia e a circula??o das mercadorias. Geralmente, os mesmos foram constru?dos em locais afastados do centro urbano na ?poca, para assim, atenderem aos princ?pios higienistas. Os surtos epid?micos que acometiam as cidades europ?ias tamb?m se manifestaram no Brasil com a chegada da c?lera atrav?s de navios vindos da Europa na d?cada de 1850 e fez com que o Governo Imperial passasse a obrigar as c?maras municipais a adotarem em suas posturas determina??es sanit?rias, previstas na legisla??o imperial de 1828, sendo estas fundamentadas na teoria higienista que em grande parte atribu?a ao veneno presente no ambiente a causa das diversas epidemias. A partir de ent?o, as pol?ticas higienistas que em grande parte possu?am na teoria miasm?tica suas bases, passaram a explicar o controle das administra??es p?blicas na comercializa??o dos g?neros aliment?cios nas cidades. As C?maras Municipais passam a ser as respons?veis pela constru??o e administra??o dos matadouros e mercados, da qual, a partir da?, os mesmos passaram a ser p?blicos, n?o mais particulares. Muitos destes edif?cios constru?dos em cidades do interior paulista j? foram demolidos, como o Matadouro Municipal de Campinas, projetado pelo renomado arquiteto-engenheiro Francisco de Paula Ramos de Azevedo. O Matadouro da Vila Mariana em S?o Paulo com projeto de Alberto Kuhlmann, inaugurado em 1887, foi preservado e ? hoje um centro cultural. Ainda, em S?o Paulo foi preservado o Mercado Municipal de Santo Amaro, inaugurado em 1897, constru?do em alvenaria de tijolos, onde este abastecia a capital de madeiras, cereais e outras mercadorias produzidas na regi?o ou recebidas de cidades pr?ximas, como Itapecerica e Embu. Na verdade, parte deste patrim?nio hoje tombado pelo CONDEPHAAT de S?o Paulo est? vinculada ? produ??o do escrit?rio do arquiteto-engenheiro Francisco de Paula Ramos de Azevedo. ? o caso do Mercado Municipal Paulistano, inaugurado em 1933, constru?do no estilo neocl?ssico. Outro exemplo ? o Mercado Municipal de Campinas, inaugurado em 1908, tamb?m projetado por Ramos de Azevedo, sendo este em estilo neomourisco. O presente trabalho apresenta um conjunto de estudos de caso de matadouros e mercados p?blicos ainda presentes em algumas cidades do interior paulista, sendo estes de significativo valor hist?rico e ainda desprotegidos pelos ?rg?os municipais.