The aim of this study was to investigate the effects of biochar amendment on soil greenhouse gas (GHG) emissions and to elucidate the mechanisms behind these effects. I investigated the suppression of soil carbon dioxide (CO2) and nitrous oxide (N2O) emissions in a bioenergy and arable crop soil, at a range of temperatures and with or without wetting/drying cycles. More detailed investigation on the underlying mechanisms focused on soil N2O emissions. I tested how biochar altered soil physico-chemical properties and the subsequent effects on soil N2O emissions. In addition, 15N pool dilution techniques were used to investigate the effect of biochar on soil N transformations. Biochar amendment significantly suppressed soil GHG emissions for two years within a bioenergy soil in the field and for several months in an arable soil. I hypothesised that soil CO2 emissions were suppressed under field conditions by a combination of mechanisms: biochar induced immobilisation of soil inorganic-N (BII), increased C-use efficiency, reduced C-mineralising enzyme activity and adsorption of CO2 to the biochar surface. Soil CO2 emissions were increased for two days following wetting soil due to the remobilisation of biochar-derived labile C within the soil. Soil N2O emissions were suppressed in laboratory incubations within several months of biochar addition due to increased soil aeration, BII or increased soil pH that reduced the soil N2O: N2 ratio; effects that varied depending on soil inorganic-N concentration and moisture content. These results are significant as they consistently demonstrate that fresh hardwood biochar has the potential to reduce soil GHG emissions over a period of up to two years in bioenergy crop soil, while simultaneously sequestering C within the soil. They also contribute greatly to understanding of the mechanisms underlying the effect of biochar addition on soil N transformations and N2O emissions within bioenergy and arable soils. This study supports the hypothesis that if scaled up, biochar amendment to soil may contribute to significant reductions in global GHG emissions, contributing to climate change mitigation. Further studies are needed to ensure that these conclusions can be extrapolated over the longer term to other field sites, using other types of biochar.

Forested riparian zones, which function as a buffer between agricultural fields and streams, filter out contaminants and sediment from the fields thereby improving water quality, cool the water with shade from trees, stabilize the stream bank and provide habitat for wildlife. However, in many agricultural areas, riparian vegetation has been removed for crop production or pasture purposes. Riparian restoration or rehabilitation is a way of restoring riparian ecosystem functions. This study examines the effect of riparian rehabilitation via tree planting along a first-order creek in Southern Ontario, 25 years after rehabilitation. Litter input, soil quality parameters and soil CO2 production rates were determined for the rehabilitated riparian zone, a grass-forb riparian zone and a natural forest riparian zone. Total litter input was 480, 580 and 295 g m-2 y-1 for the rehabilitated riparian zone, grass riparian zone and forest riparian zone, respectively. Soil bulk density was higher and hydraulic conductivity was lower for the rehabilitated riparian zone compared to the grass riparian zone and forest riparian zone. The concentration and soil stock of organic carbon and total nitrogen was lowest for the rehabilitated riparian zone compared to the grass riparian zone and forest riparian zone which were similar. The effect of riparian zone on soil CO2 production rates varied over the season. From spring to mid-summer, rates were 167, 224 and 104 mg C m-2 h-1 for the rehabilitated riparian zone, grass riparian zone and forest riparian zone, respectively. Soil CO2 production rates did not differ significantly (p < 0.05) between riparian zones for late summer and fall sampling dates. Soil CO2 production rates were significantly negatively correlated with soil C/N and positively correlated with soil pH and litter input. Soil CO2 production rates were positively correlated with soil temperature (r = 0.32) and negatively correlated with soil moisture (r = -0.48). Of the three riparian zones, the natural forest riparian zone exhibited the least amount of seasonal fluctuation for soil CO2 production rates, soil moisture and temperature. Results from this research indicated that more time is needed before soil quality and soil CO2 production rates of the rehabilitated riparian zone reach values similar to the natural forest riparian zone.

The distributions of calcite rich horizons within dryland soils are commonly used as paleoclimate proxies. Comprehensive conceptual and mathematical models of calcite accumulation in soils are required to accurately interpret and calibrate these proxies. A conceptual model for calcite accumulation is already well established: As water percolates through a soil, it dissolves minerals, such as calcite, transporting the soluble minerals downward. As soil water is removed by evaporation and transpiration, the water solution becomes supersaturated resulting in precipitation of calcite at depth. The impacts of dynamic plant growth and microbial respiration have not yet been simulated in numerical models for calcite accumulation but are likely important because of their influence on variables governing calcite solubility. The soil hydraulic modeling software, HYDRUS-1D, simulates water and solute transfer through a soil column, accounting for variations in all previously studied variables (temperature, water content, soil pCO₂) while additionally simulating vegetation-soil interactions. Five separate sensitivity studies were conducted to determine the importance for calcite accumulation of 1) soil texture, 2) plant growth, 3) plant phenology, 4) atmospheric CO₂ concentrations, and 5) the proximal variables that control calcite dissolution and precipitation: soil CO₂, soil water content, and soil temperature. In each modeling simulation, calcite was leached from the top several cm and redistributed deeper in the soil after 20 years. Soils with courser texture yield deeper (+20cm), more diffuse calcite horizons, as did simulations with bare soil compared to vegetated soil. The phenology of plant communities (late spring versus late summer growth) resulted in soil calcite accumulation at temperatures differing by at least 10°C. Changes in atmospheric CO₂ concentrations do not affect the soil calcite distribution. Variations in concentration of soil CO₂, rather than soil water content, have the greatest direct effect on calcite solubility. The most significant time periods of annual accumulation also corresponded with positive water fluxes resulting from high matric potential at the surface. Transpiration and evaporation moisture sinks caused solution to travel upward from higher to lower soil CO₂ concentrations, causing CO₂ de-gassing and calcite accumulation. This pathway describes a new qualitative mechanism for soil calcite formation and should be included in the conceptual model. / text

Forested riparian zones, which function as a buffer between agricultural fields and streams, filter out contaminants and sediment from the fields thereby improving water quality, cool the water with shade from trees, stabilize the stream bank and provide habitat for wildlife. However, in many agricultural areas, riparian vegetation has been removed for crop production or pasture purposes. Riparian restoration or rehabilitation is a way of restoring riparian ecosystem functions. This study examines the effect of riparian rehabilitation via tree planting along a first-order creek in Southern Ontario, 25 years after rehabilitation. Litter input, soil quality parameters and soil CO2 production rates were determined for the rehabilitated riparian zone, a grass-forb riparian zone and a natural forest riparian zone. Total litter input was 480, 580 and 295 g m-2 y-1 for the rehabilitated riparian zone, grass riparian zone and forest riparian zone, respectively. Soil bulk density was higher and hydraulic conductivity was lower for the rehabilitated riparian zone compared to the grass riparian zone and forest riparian zone. The concentration and soil stock of organic carbon and total nitrogen was lowest for the rehabilitated riparian zone compared to the grass riparian zone and forest riparian zone which were similar. The effect of riparian zone on soil CO2 production rates varied over the season. From spring to mid-summer, rates were 167, 224 and 104 mg C m-2 h-1 for the rehabilitated riparian zone, grass riparian zone and forest riparian zone, respectively. Soil CO2 production rates did not differ significantly (p < 0.05) between riparian zones for late summer and fall sampling dates. Soil CO2 production rates were significantly negatively correlated with soil C/N and positively correlated with soil pH and litter input. Soil CO2 production rates were positively correlated with soil temperature (r = 0.32) and negatively correlated with soil moisture (r = -0.48). Of the three riparian zones, the natural forest riparian zone exhibited the least amount of seasonal fluctuation for soil CO2 production rates, soil moisture and temperature. Results from this research indicated that more time is needed before soil quality and soil CO2 production rates of the rehabilitated riparian zone reach values similar to the natural forest riparian zone.

Soil carbon dioxide efflux resulting from microbial and root respiration is a major component of the forest carbon cycle. We undertook this investigation to better understand the nature of soil carbon dioxide efflux of plantation loblolly pine, an important ecological and economical resource in the southeastern United States. Specifically, we hoped to learn how soil carbon dioxide efflux differs both spatially and temporally for four age classes of plantation loblolly pine on the Virginia piedmont. During a 12-month period, soil carbon dioxide efflux was repeatedly measured for four age classes of plantation loblolly pine using a dynamic, closed-chamber infrared gas analyzer. The age classes examined were 1- to 2-year-old, 4- to 6-year-old, 8- to 12-year-old, and 20- to 25-year-old stands. Mean soil carbon dioxide efflux rates measured during the 12-month study were 1.72, 2.58, 2.84, and 2.90 micromole/sq m/s for 1- to 2-year-old, 4- to 6-year-old, 8- to 12-year-old, and 20- to 25-year-old stands, respectively. Stand age had a significant effect on efflux rate during 10 of the 12 monthly sampling sessions. Additionally, mean efflux rates were consistently higher near the tree and a significant positional difference was detected during 8 of the 12 monthly sampling sessions. Mean soil carbon dioxide efflux rates, by position, for the 12-month study were 2.72 and 2.28 micromole/sq m/s for the near and away measurement positions, respectively. Based on monthly mean soil carbon dioxide efflux rates, annual carbon losses were estimated at 651, 976, 1074, and 1082 g C/sq m/yr for 1- to 2-year-old, 4- to 6-year-old, 8- to 12-year-old, and 20- to 25-year-old stands, respectively. Regression analysis was used to examine the influence of soil and climatic factors on seasonal changes in soil carbon dioxide efflux. The most influential factors affecting soil carbon dioxide efflux during the 12-month study were soil temperature, soil moisture, stand age, and measurement position. We believe respiring roots significantly influence soil carbon dioxide efflux of plantation loblolly pine and account for differences observed between stands of different ages as well as spatial differences observed within a given stand. / Master of Science