In this study the growth and yield response of Solanum retroflexum Dun. (nightshade) and Brassica rapa L. subsp. chinensis (non-heading Chinese cabbage) to N, P and K availability in the soil and the interaction effects of these three nutrients were determined by means of pot experiments in a greenhouse. S. retroflexum was most sensitive to the availability of nitrogen in the soil. Sufficient nitrogen needed to be available to achieve optimum growth but adding too much adversely affected biomass production, suggesting a fairly narrow optimum range for nitrogen availability. The production of the crop was also dependent on the adequate availability of phosphorus and potassium but any adverse effects due to excess availability were less distinct than for nitrogen. In the case of B. rapa subsp. chinensis, an optimum availability range was identified for N and K and a critical level of availability for P. The decline in biomass production caused by adding N in excess of the optimum was reversed by applying both P and K at rates that were in excess of the respective optima.

Thesis (M. Sc. Agric.(Plant Production)) -- University of Limpopo, 2019 / Nightshade (Solanum retroflexum Dun.) is among the most important indigenous leafy vegetables in Vhembe District, Limpopo Province, South Africa, due to its high values of beta-carotene, vitamin E, folic acid, ascorbic acid, calcium, iron and protein. Vhembe District occurs in the tropical regions of Limpopo Province and the production of vegetables is dependent upon the availability of irrigation water. An Integrated Drip Irrigation System (IDIS) and a 3S planter were developed to save water by planting several plants/hole of drip irrigation system. The subsystems in IDIS allow for the production of different crops with different water requirements, whereas the 3S planter can be used for planting from one to nine plants/hole of drip irrigation system. Also, the subsystems could be used in assessing irrigation interval for crops under various planting densities. The interaction of irrigation interval and planting density of S. retroflexum had not been documented. The objective of this study, therefore, was to determine the interactive effects of irrigation interval and planting density on biomass yield and chemical nutrient elements (summer harvest only) of S. retroflexum under field conditions. The irrigation interval and planting density/hole were arranged in a split-plot experimental design, with eight replications. The main plot was irrigation interval and the subplot was the planting densities. Harvesting was done twice for both summer and winter experiments. The first harvest (H1) was done at 6 weeks after transplanting, with the second harvest (H2) being done at six weeks after the first harvest. Fresh shoots were oven-dried at 60°C for 72 h for the determination of dry matter. Mature leaves were powdered and analysed for mineral content (Ca, P, K, Mg, Na, Fe, Zn, Mn and Cu) using the ICPE-9000. Data were subjected to analysis of variance using SAS software. In the summer experiment, the interaction was significant (P ≤ 0.05) for dry shoot mass at H1 and H2. However, the contribution of xv the interaction in the total treatment variation (TTV) of the variable was negligent and therefore, only single factors were reported. Irrigation interval and planting density had highly significant (P ≤ 0.01) effects on plant variables during H1 and H2 in summer and winter. However, irrigation interval effects for dry shoot mass were not significant for summer H2. Interaction effects were significant for Ca, P, K, Mg, Mn and Cu in leaf tissues during summer H1, but were not significant for Na, Fe and Zn. Also, irrigation interval was significant for Ca, Mg, P, K, Na, Fe, Zn, Mn and Cu during summer H1, whereas planting density had no significant effects for all chemical nutrients except for Ca, P and K during summer H1. Dry shoot mass of S. retroflexum increased linearly with increasing irrigation interval and planting density. Results suggested that most nutrient elements increased with deficit irrigation water and higher planting density, whilst P decreased under high planting density. The study showed that there is a high potential for saving water through longer irrigation intervals and produce good high yields at a higher planting density. In conclusion, the use of IDIS and 3S planter to promote growth and accumulation of essential nutrient elements on S. retroflexum demonstrated that longer irrigation interval and higher plant density per drip irrigation hole could be suitable for cultivation of this indigenous vegetable. The recommendation of this study is that higher planting density and longer irrigation intervals are key determinants of higher biomass yield and water saving strategies for large-scale production of the crop. Further, the mineral composition of the crop was under the influence of higher planting density and irrigation intervals.

Thesis (M. A. Agriculture (Plant Protection)) -- University of Limpopo, 2019 / The successful cultivation of nightshade (Solanum retroflexum) as a leafy vegetable with the nutritional potential of contributing to food security in marginalised communities of Limpopo Province could be limited by high population densities of root-knot (Meloidogyne species) nematodes. However, the use of Nemarioc-AL/AG and Nemafric-BL/BG phytonematicides in suppressing nematodes and not being phytotoxic requires the empirically-developed non-phytotoxic concentration, technically referred to as Mean Concentration Stimulation Point (MCSP). The MCSP, developed using the Curve-fitting Allelochemical Response Data (CARD) computer-based model, is crop-specific, hence it should be developed for every crop. The objective of this study was to investigate the influence of Nemarioc-AL/AG and Nemafric-BL/BG phytonematicides on growth of nightshade, accumulation of essential nutrient elements and cucurbitacin residues in nightshade leaves. Microplots were established by inserting 20-cm-diameter plastic pots into 10-cm-deep holes at 0.6 m intra-row and 0.6 m inter-row spacing. Each pot was filled with 10 000 cm3 steam-pasteurised river sand and Hygromix at 3:1. After establishment, Nemarioc-AL and Nemafric-BL phytonematicides were applied at 7-day interval, whereas, Nemarioc-AG and Nemafric-BG phytonematicides were only applied at planting. Two separate experiments for Nemarioc-AL and Nemafric-BL phytonematicides were conducted in summer (November-January) 2017/2018 under microplot conditions with each comprising treatments namely; 0, 2, 4, 8, 16, 32 and 64%, similarly, two separate experiments for the following phytonematicides, Nemarioc-AG and Nemafric-BG comprised treatments namely; 0, 2, 4, 6, 8, 10 and 12 g arranged in a randomised complete block design (RCBD), with 12 replications. The nutrient elements in leaf tissues of nightshade were analysed using the inductively coupled plasma optical emission spectrometry (ICPE-9000) while, cucurbitacin A and B were xii each quantified using the isocratic elution Shimadzu HPLC Prominence with Shimadzu CTO-20A diode array detector. Plant growth and nutrient elements variables were subjected to the CARD computer-based model to generate biological indices to generate the curves, quadratic equations and the related biological indices (Dm, Rh, k) (Liu et al., 2003). The MCSP values were calculated using the biological indices of plant or nutrient element variables which, along with increasing concentration of Nemarioc-AL, Nemafric BL, Nemarioc-AG and Nemafric-BG phytonematicides, exhibited positive quadratic relations, with R2 ≥ 25. Using cucurbitacin A and B standards, residues of Nemarioc AL/AG and Nemafric-BL/BG phytonematicides, were not detected in nightshade leaves, respectively. Dry root mass and dry shoot mass of nightshade over increasing concentration of Nemarioc-AL phytonematicide each exhibited a quadratic relationship, with the models explained by 93 and 61%, respectively. Dry root mass, dry shoot mass, plant height, chlorophyll content and stem diameter against increasing concentration of Nemafric-BL phytonematicide each exhibited positive quadratic relationships with the models explained by 95, 72, 65, 78 and 62%, respectively. Plant height, stem diameter and dry root mass against increasing concentration of Nemarioc-AG phytonematicide each exhibited positive quadratic relationships with their models explained by 93, 88 and 91%, respectively. Dry shoot mass and stem diameter against increasing concentration of Nemafric-BG phytonematicide each exhibited positive quadratic relationships with their models explained by 94 and 84%, respectively. Na, Fe and K over increasing concentration of Nemarioc-AL phytonematicide each exhibited positive quadratic relationships with their associations explained by 96, 91 and 95%, respectively. Zn over increasing concentration of Nemafric-BL phytonematicide exhibited positive quadratic relationship with the model explained by 98%. Fe over increasing concentration of Nemarioc-AG phytonematicide exhibited positive quadratic xiii relationship with the association explained by 91%. Fe, Na, K and Zn over increasing concentration of Nemafric-BG phytonematicide each exhibited positive quadratic relationships with their associations explained by 81, 90, 80 and 89%, respectively, whereas, on the contrary, Zn over increasing concentration of Nemarioc-AG phytonematicide exhibited negative quadratic relationship with the association explained by 96%. Significant (P ≤ 0.05) plant variables were subjected to CARD, to generate biological indices which were used to compute the MCSP using the relation: MCSP = Dm + Rh/2 and the overall sensitivity value (∑k). In Nemarioc-AL phytonematicide trial, MCSP = 3.02% and ∑k = 1 for plant variables, whereas, MCSP and ∑k for nutrient elements were 12.09% and 1, respectively. In Nemafric-BL phytonematicide trial, MCSP = 3.08% and ∑k = 0 for plant variables, while MCSP = 2484.14% and ∑k = 0 for nutrient elements. In Nemarioc-AG phytonematicide trial, MCSP = 3.47 g and ∑k = 0 for plant variables, whereas, for nutrient elements MCSP = 8.49 g and ∑k = 1. In Nemafric-BG phytonematicide trial, MCSP = 4.70 g and ∑k = 0 for plant variables, whereas, MCSP =723.75 g and ∑k = 1 for nutrient elements. In conclusion, the application of Nemarioc-AL/AG and Nemafric-BL/BG phytonematicides had the ability to stimulate the growth of nightshade and enhance the accumulation of the selected nutrient elements without leaving cucurbitacin chemical residues in leaf tissues of nightshade. / National Research Foundation (NRF) and the Land Bank Chair of Agriculture

Thesis (M. Agricultural Management (Agronomy)) -- University of Limpopo, 2018 / A survey study identified indigenous leafy vegetables (ILVs) utilized by rural communities in Limpopo Province in the three districts of Capricorn, Sekhukhune and Vhembe focusing on their availability, agronomic practices, marketing, medicinal and cultural roles, as well as their nutritional value. An ILV that was highly preferred and with good marketing potential was identified for further studies on its response to planting density and nitrogen fertilizer rate. A questionnaire survey was used to gather information about types of ILVs utilised, their production practices, marketing of ILVs and their importance in medicinal and cultural roles. Data collected were subjected to analysis using the Statistical Package for Social Sciences (SPSS) using descriptive statistics. Results showed that there were 45 different types of ILVs identified from the three districts in Limpopo Province. Farmers indicated that cultivation of these vegetables was mostly done from October to January in Sekhukhune and Capricorn district while in Vhembe it was practiced all year round. Most of farmers in Vhembe district used inorganic fertilizer during planting while farmers in Sekhukhune and Capricorn districts used organic fertilizers such as cattle manure. Irrigation was commonly used in Vhembe district through furrow irrigation system whereas in Capricorn and Sekhukhune districts most farmers relied on rainfall. Farmers indicated that harvesting was done at an early growing stage of the crop. Indigenous leafy vegetables were mostly marketed in local communities. Several ILVs were identified as preferred and regularly consumed as vegetables. Spider plant (Cleome gynandra) and nightshade (Solanum retroflexum) were identified as the most consumed ILVs in the three districts. Comparing the market potential of the two vegetables, S. retroflexum was chosen for further studies on its response to plant density and nitrogen fertilizer rate. The first study investigated the effect of varying plant density (inter and intra-row spacing) and the second study evaluated the response of intra-row spacing and nitrogen fertilizer (LAN-28%N) rate on growth and yield of S. retroflexum during 2014 (April to May) and 2015 (March to May) growing seasons. The plant density experiment was laid out as a 3 x 4 factorial arrangement in a randomised complete block design (RCBD) with three replications. The treatment factors were 3 inter-row spacings of 30, 45 and 60 cm and 4 intra-row spacings of vi 7.5, 15, 22.5 and 30 cm. The data collected included plant height, plant vigour, number of leaves and branches per plant as well as plant leaf yield. Data were subjected to analysis of variance (ANOVA) using STATISTIX 10.0 package and mean treatments were separated using Turkey HSD at 5% probability level. The results revealed that growth parameters (plant height, plant vigour, number of branches and leaves) and plant leaf yield were significantly influenced by the combined inter and intra-row spacings. Closer inter-row spacings of 30 cm and 45 cm, and intra-row spacings of 7.5 and 15 cm produced the highest values of parameters and plant leaf yield. The combined spacings of 30 x 7.5 cm produced the highest plant leaf yield. The second study was also laid out as a randomised complete block design (RCBD) with three replications. The treatments were 6 x 2 factorial arrangement: 6 levels of nitrogen (LAN-28%N) at 0, 20, 40, 60, 80 and 100 kg N/ha and two intra-row spacings of 15 and 30cm. Inter-row spacing of 30 cm was used. The nitrogen fertilizer was applied a week after transplanting and repeated a week after first harvesting. Data was subjected to analysis of variance (ANOVA) using STATISTIX 10.0 package. Where significant differences were detected, means were separated using Turkey HSD at 5% propability level. The results suggested that both nitrogen fertilizer and spacing can be used to enhance growth and leaf yield of S. retroflexum vegetable. Nitrogen fertilizer rate and plant density significantly (P≤0.05) affected plant growth and plant leaf yields. Growth parameters and leaf yield were optimised using closer spacing of 15 cm and applying nitrogen at 60 kg N/ha. The application of 60 kg N/ha and 15 cm spacing was therefore recommended for the production of S. retroflexum as a vegetable, if planted at 30 cm inter-row spacing. Key words: Indigenous leafy vegetables, agronomic practices, Solanum retroflexum, planting density, nitrogen fertilizer, planting date