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Activated Sludge as Renewable Fuels and Oleochemicals FeedstockRevellame, Emmanuel Durante 09 December 2011 (has links)
The utilization of activated sludge as feedstock for biofuel and oleochemical production was investigated. Initial studies included optimization of biodiesel production from this feedstock through in situ transesterification. Results of these studies indicated that activated sludge biodiesel is not economically viable. This was primarily due to relatively low yields and the high economics of feedstock dewatering. Strategies to increase biofuel yield from activated sludge were then evaluated. Bacterial species present in activated sludge are known to produce a wide variety of lipidic compounds as carbon and energy storage material and as components of their cellular structures. In addition to lipidic compounds, activated sludge bacteria might also contain other compounds depending on wastewater characteristics. Among these bacterial compounds, only the saponifiable ones can be converted to biodiesel. The unsaponifiable compounds present in the activated sludge are also important, not only for biofuel production, but also for a wide variety of applications. Characterization of lipids in activated sludge revealed that it contains significant amount of polyhydroxyalkanoates, wax esters, acylglycerides and fatty acids. It also contains Template Created By: James Nail 2010 sterols, steryl esters and phospholipids as well as small but detectable amounts of hydrocarbons. This indicated that activated sludge could be also an inexpensive source of oleochemicals. Another strategy that was evaluated was lipid-enhancement by fermentation of activated sludge. Since the majority of products from petroleum oil are used as transportation fuel, the aim here was to increase the saponifiable lipids in activated sludge bacteria by applying a biochemical stimulus (i.e. high C:N ratio). Results showed that application of this stimulus increased the amount of saponifiable lipids, particularly triacyglycerides, in the activated sludge. Furthermore, fermentation homogenized the lipids in the sludge regardless of its source. This solidified the concept of utilizing wastewater treatment facilities as biorefineries. To support the utilization of other compounds in raw activated sludge for biofuel production, a model compound was chosen for catalytic cracking experiments. Results indicated that catalytic cracking of 1-octadecanol over H+ZSM5 proceeds via dehydration, producing octadecene. The octadecene then undergoes a series of reactions including β-C─C bond scission, alkylation, oligomerization, dehydrocyclization and aromatization producing aromatics, paraffins and olefins suitable for fuel applications.
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On Chip Preconcentration and Labeling of Protein Biomarkers Using Monolithic Columns, Device Fabrication, Optimization, and AutomationYang, Rui 01 February 2014 (has links) (PDF)
Detection of disease specific biomarkers is of great importance in diagnosis and treatment of diseases. Modern bioanalytical techniques, such as liquid chromatography with mass spectrometry (LC-MS), have the ability to identify biomarkers, but their cost and scalability are two main drawbacks. Enzyme-linked immunosorbent assay (ELISA) is another potential tool, but it works best for proteins, rather than peptide biomarkers. Recently, microfluidics has emerged as a promising technique due to its small fluid volume consumption, rapidness, low fabrication cost, portability and versatility. Therefore, it shows prominent potential in the analysis of disease specific biomarkers. In this thesis, microfluidic systems that integrate monolith columns for preconcentration and on-chip labeling are developed to analyze several protein biomarkers. I have successfully fabricated cyclic olefin copolymer (COC) microfluidic devices with standard micromachining techniques. Monoliths are prepared in situ in microchannels via photopolymerization, and the physical properties of monoliths are optimized by varying the composition and concentration of monomers to achieve better flow and extraction. On-chip labeling of protein biomarkers is achieved by driving solution through the monolith using voltage and incubating fluorescent dye with protein retained in the monolith. Subsequently, the labeled proteins are eluted by applying voltages to reservoirs on the microdevice and detected by laser-induced fluorescence. Finally, automation of on-chip preconcentration and labeling is successfully demonstrated.
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Design and Development of 2-Functionalized Calix[4]arenes and Their Investigation in the Separation of LanthanidesMenon, Sreejit Rajiv, Menon January 2016 (has links)
No description available.
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Commercial Applicability of an Innovative Anthocyanin Purification Technique, Utilizing Mixed-Mode Solid-Phase ExtractionSimmons, Steven Tyler 20 June 2012 (has links)
No description available.
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Evaluation of SPEC®•Microcolumns for the extraction of ranitidine from urineBlankenship, Donna A. 05 September 2009 (has links)
Solid phase extraction (SPE) has become a popular sample preparation method for the extraction of analytes from complex matrices. Solid phase extraction overcomes such disadvantages as large solvent usage and waste, large amounts of time spent in sample preparation and labor intensive methods with little hope of automation. In this report, SPEC®⋅ Microcolumns are evaluated for the extraction of Zantac® (ranitidine hydrochloride) from urine for high performance liquid chromatography (HPLC) analysis.
SPEC®⋅Microcolumns are rigid disc structures with glass fiber impregnated with silica gel. These microcolumns look like packed-bed SPE cartridges but have the advantages of low void volumes and low bed mass that give clean extracts with the use of small amounts of solvents. An SPE method was developed and evaluated for the recovery of Zantac® from water and spiked urine. Percent recoveries and matrix interferences are reported. The limit of detection and analytical range tor the HPLC method of analysis with ultraviolet detection will be presented. / Master of Science
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Detection, analysis, and photocatalytic destruction of the freshwater taint compound geosminBellu, Edmund January 2007 (has links)
A significant issue affecting the aquaculture and water industries is the presence of off-flavour compounds in water, which cause problems by imparting an undesirable earthy/musty flavour and smell to water and fish. Two predominant off-flavour compounds are geosmin (GSM) and 2-methylisoborneol (MIB). These compounds are produced by several varieties of cyanobacteria and actinomycetes as metabolic products and can be detected by humans at concentrations as low as 0.015 mg L-1. Removal of GSM and MIB from potable waters has proven to be inefficient using standard water treatment such as filtration, coagulation, flocculation, sedimentation and chlorination. Activated carbon and membrane processes can physically remove GSM and MIB, but do not destroy them, and ozone treatment can be expensive. Titanium dioxide (TiO2) photocatalysis has recently been demonstrated to rapidly degrade GSM and MIB. When the semiconductor catalyst is illuminated with ultraviolet light simultaneous oxidation and reduction reactions occur. Pollutants are broken down into mineral acids, carbon dioxide and water. This study was conducted to determine if TiO2 photocatalysis, using a pelleted form of TiO2 called Hombikat K01/C, was a suitable method for the treatment of potable water. Additionally an analytical method was developed to rapidily analyse the large number of samples generated. Two reactors, a bench scale batch reactor and pilot scale flow reactor, were developed and used to evaluate the efficacy of Hombikat K01/C TiO2 photocatalysis in degrading GSM. The batch reactor, containing Hombikat K01/C, was used to investigate the effect of numerous experimental variables on the photocatalysis of GSM, including initial substrate concentration, pH, light intensity, aeration rate, the presence of additional reactants, and catalysis conducted in deuterated water. GSM was rapidly degraded using the TiO2 batch reactor, with the rate of GSM degradation most affected by light intensity and additional reactants, though pH also had a notable effect. A kinetic isotope effect of 1.61 was observed for the destruction of GSM using Hombikat K01/C TiO2. The flow reactor was also found to efficiently degrade GSM in raw waters. The rate of GSM destruction was found to be significantly lowered by UV shielding of the catalyst, caused by constituents of raw the water used, and the presence of additional reactants. The pilot scale flow reactor was also successfully evaluated in Denmark using gesomin contaminated water from an eel farm
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Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine WastesAbdulrheem, Ali Jamal 01 April 2017 (has links)
The spread of tetracyclines through agricultural systems is causing the present bacteria to develop antibiotic resistance. The spread of this bacteria, as well as the tetracycline antibiotics in the environment is dangerous because these antibiotics pose health hazards for humans. The overuse of antibiotics, which are added to livestock feed, results in the antibiotics being released into the environment via animal feces. In this research, we have attempted to design an analytical method to isolate antibiotics from agricultural wastes with subsequent detection using liquid chromatography and mass spectrometry (LC-MS). The antibiotics investigated in this study were tetracycline, chlortetracycline, and oxytetracycline.
The analytical procedure involves mixing the agricultural samples with an organic solvent, such as methanol, which solubilizes these antibiotics. Next, samples are centrifuged to remove solid particulates. A polymeric weak cation cartridge was used to concentrate and separate the antibiotics from the unwanted organic chemical compounds found in the samples. The antibiotics were released with methanol with small amounts of acid and then detected and quantified using LC-MS and high performance liquid chromatography with a photodiode array detector (HPLC-PDA).
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Stanovení estrogenních polutantů v reálné vodné matrici metodou HPLC-UV po extrakci na tuhé fázi. / Determination of estrogen pollutants in real water sample by HPLC-UV after solid phase extraction.Kozlík, Petr January 2010 (has links)
4 Abstract Estrogens are considered to belong to chemicals that negatively affect the endocrine system, even if present at very low concentrations. They are discharged into environment as a result of an increasing application of drugs etc. This work is focused on the separation and quantification of five estrogens, namely estrone (E1), 17β-estradiol (βE2), 17α-estradiol (αE2), 17α-ethynylestradiol (EE2) and estriol (E3) in natural water samples by HPLC-UV method. The chromatographic system consisted of a C18 stationary phase (SunFire® C18, 150 x 4.6 mm, octadecyl bounded to silica gel, particle size 5 µm) and binary mobile phase of acetonitrile/water in various ratios in isocratic separation mode. The effect of acetonitrile content in the mobile phase and flow rate of the mobile phase on retention and separation parameters was tested. Under the optimized separation conditions (acetonitrile/water 40/60 (v/v), 1.3 ml/min), all the compounds were baseline resolved and eluted within 15 min. These experimental conditions were applied to the calibration measurements which were carried out within the concentration range from 0.001 to 1 mg/ml. Limits of detection (LOD) and limits of quantification (LOQ) for the individual estrogens and their mixture (standards dissolved in methanol) were determinated. The detection...
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Fotodegradação do contaminante emergente 2-(tiocianometiltio) benzotiazol (TCMTB) por meio de fotólise diretaBertoldi, Crislaine Fabiana January 2017 (has links)
Os contaminantes emergentes são considerados compostos onipresentes em águas, portanto investigar a degradação e comportamento dessas substâncias torna-se necessária, pois é reportado que estes compostos causam efeitos adversos em seres vivos. O composto 2-(tiocianometiltio) benzotiazol (TCMTB), considerado um contaminante emergente, é amplamente empregado na indústria do couro como biocida com a finalidade de inibir o desenvolvimento de microrganismos na pele. Sendo assim, o objetivo do presente trabalho foi estudar a degradação do contaminante emergente TCMTB, por meio das técnicas de fotólise direta com radiação UV, radiação solar e oxidação com ozônio. Experimentos de fotólise direta do TCMTB, em soluções aquosas com diferentes concentrações, em efluente do processo de remolho e efluente simulado do remolho, foram conduzidos em reator com lâmpada de vapor de mercúrio (250 W). O teste de hidrólise foi realizado protegido da luz, a temperatura ambiente com diferentes concentrações do TCMTB para observar o comportamento do contaminante na ausência de luz. O maior coeficiente de absorção molar foi medido e identificado em 220 e 280 nm como 20489 e 11317 M-1 cm-1, respectivamente, para pH 5,0. Os resultados experimentais da fotodegradação mostraram que TCMTB foi rapidamente degradado por fotólise direta em soluções aquosas em 30 min de tratamento fotolítico. Os resultados do estudo do pH, demonstraram que pH interfere no processo fotoquímico, uma vez que em condições alcalinas o composto é mais estável e a taxa de fotodegradação diminui. Os ensaios com o efluente do processo de remolho mostraram que a degradação do TCMTB tem comportamento semelhante às soluções aquosas. O efluente simulado do remolho mostrou que uma alta concentração do contaminante leva a um maior tempo de irradiação de luz para a degradação. A aplicação de luz natural evidenciou degradação mais lenta, mas ainda assim, foi possível observar degradação de até 96% para a concentração de 6 mg L-1 em 420 min. A utilização do oxidante ozônio como tratamento, alcançou 40% de remoção do contaminante em 30 min, assinalando a alta estabilidade do composto. Portanto, este trabalho aponta o potencial do uso de fotólise direta (luz v artificial), ou radiação solar (luz natural) para a degradação de contaminantes emergentes como o 2 (tiocianometiltio) benzotiazol (TCMTB). / Emerging contaminants are considered omnipresent compounds in water, thus investigate the degradation and behavior of these substances becomes necessary as it is reported that these compounds cause adverse effects on living beings. The 2- (thiocyanomethylthio) benzothiazole compound (TCMTB), considered an emerging contaminant, is widely used in the leather industry as a microbicide for the purpose of inhibiting the development of microorganisms in the skin and leather. In this context, the purpose of the present work was to study the degradation of the emerging contaminant TCMTB by direct photolysis with UV radiation, solar radiation and ozone. Experiments of direct photolysis of the TCMTB in aqueous solutions with different concentrations, in the effluent from the soaking process were conducted in a reactor with mercury vapor lamp (250 W). The hydrolysis test was performed protected from light at room temperature with different concentrations of TCMTB to observe the behavior of the contaminant in the absence of light. The highest molar absorption coefficient was measured and identified at 220 and 280 nm as 20489 and 11317 M-1 cm -1, respectively, at pH 5.0. The experimental results of photodegradation showed that TCMTB was rapidly degraded by direct photolysis in aqueous solutions in 30 min of photolytic treatment. The results of the pH study showed that pH interfered in the photochemical process, since under alkaline conditions the compound is more stable and the photodegradation rate decreases. Assays of the direct photolysis in effluent from the soaking process have shown that TCMTB degradation behaves similarly to aqueous solutions. The direct photolysis of the the simulated effluent from the soaking showed that a high concentration of the contaminant leads to a longer time of light irradiation for degradation. The application of natural light evidenced slower degradation, however, it was possible to observe degradation of up to 96% for the 6 mg L-1 concentration with 420 min. The use of the ozone oxidant as a treatment, achieved 40% removal of the contaminant for 30 min of treatment, indicating the high stability of the compound. Therefore, this work highlights the potential of the use of direct photolysis (artificial light), or solar radiation (natural light) for the vii degradation of emerging contaminants such as 2 (thiocyanomethylthio) benzothiazole (TCMTB).
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[en] USE OF POLYURETHANE FOAM ON INDIUM EXTRACTION FROM IODIDE MEDIUM / [pt] USO DE ESPUMA DE POLIURETANO NA EXTRAÇÃO DE ÍNDIO DE MEIO IODETOELIZABETH DE MAGALHAES MASSENA FERREIRA 13 April 2004 (has links)
[pt] Este trabalho apresenta um estudo sistemático da sorção
de índio de meio iodeto, utilizando espuma de poliuretano
à base de poliéter comercial como extrator em fase
sólida, visando à pré-concentração e/ou separação de
índio de diferentes matrizes de amostras. As
características físico-químicas de sorção do sistema
foram investigadas por processo em batelada, apresentando
rápida cinética e máxima sorção numa faixa significativa
de meio ácido ou básico, com coeficientes de distribuição
acima de 104 L kg-1 ([KI] maior ou igual 0,6 mol L-1). A
análise do equilíbrio de sorção indicou o tetraiodo-
indato (MinI4) como a principal espécie sorvida na matriz
da espuma; os mecanismos de sorção mais prováveis foram
discutidos. A isoterma de Langmuir foi ajustada com êxito
sendo obtido um valor de (1,55 mais ou menos 0,02)x10-1
mol kg-1 para a
capacidade de saturação. O efeito da temperatura foi
avaliado indicando um processo espontâneo e exotérmico
caracterizado por sorção química. A reextração do índio
da espuma com solução de HCl 1,0 mol L-1 em meio etanol
50% apresentou rápida cinética (10 minutos) e eficiência
(97%). O sistema pode ser utilizado para a separação de
traços de índio de grandes quantidades de Al, Fe, Zn, Ni,
Co, Mn e Ga. Cd e Pb são co-extraídos. Ácido cítrico, F-
e EDTA interferem na sorção do índio; tiosulfato de sódio
(menor 0,1 mol L-1) e ácido ascórbio (até 0,8 mol L-1)
não
interferem. O sistema foi avaliado na determinaço de In
na presença de 105 vezes Zn, Al, Fe pelo método de adição
e recuperação de índio. Os elementos determinados por ICP-
OES apresentaram fatores de separação, da ordem de 7,0 x
102 para Zn/In, 1,0x105 para Fe/In e de 5,5x104 para
Al/In, com eficiente recuperação de índio. A metodologia
desenvolvida foi aplicada para determinar índio por ICP-
OES em material de referência padrão de zinco metálico
(NIST), apresentando concordância compatível com o valor
certificado, para um nível de confiança de 95%. / [en] This work shows a systematic study on indium iodide
sorption using commercial polyether-based polyurethane foam
as a solid phase extractor in order to preconcentrate and/
or separate indium from different sample matrices. The
physico-chemical characteristics of the system sorption
were investigated for the batch process. The system has
shown rapid kinetic and maximum sorption from a meaningful
range of either acid or basic medium and achieves
distribution ratios above 104 L kg-1 ([KI] maior ou igual
0.6 mol L-1).
Sorption equilibrium analysis indicates the tetraiodo-
indate (MInI4) as the main species sorbed on the foam
matrix; the more probable mechanisms of sorption were
discussed. Langmuir isotherm was successfully fitted and a
value of (1.55 mais ou menos 0.02)x10-1 mol kg-1 was
obtained for the
saturation capacity. The temperature effect was evaluated
and indicates a spontaneous and exothermic chemisorption
process favored at low temperatures. The indium
reextraction from the foam using a HCl 0.1 mol L-1 in
ethanol 50% medium solution has also shown a rapid kinetic
(10 minutes) and efficiency (97%). The system should be
used to separate large quantities of Al, Fe, Zn, Ni, Co, Mn
and Ga from trace indium. Cd and Pb are coextracted with
indium. Citric acid, F- and EDTA interfere on indium
sorption; sodium thiosulfate (menor ou igual 0.1 mol L-1)
and ascorbic
acid (up to 0.1 mol L-1) do not interfere. The system was
evaluated in the determination of indium in the presence of
105 times Zn, Al and Fe by indium addition and recovery
technique. The elements determination by ICP-OES showed
separation factors around 7.0x102 for Zn/In, 1.0X105 for
Fe/In and of 5.5x104 for Al/In, with an efficient recovery
of indium. The developed methodology was applied to
determine indium in metallic zinc standard reference
material (NIST) by ICP-OES and has shown compatible
agreement with the certified value at a 95% confidence
level.
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