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No description available.

No description available.

Chicken and pufferfish genomic libraries were screened with the intent of isolating PEA3 orthologues from evolutionarily removed vertebrate species. The chicken PEA3 gene was found to reside within 15 kb of genomic sequence, and approximately 2 kb of promoter sequence has been identified. Although the pufferfish PEA3 genomic sequence has yet to be completed, exons 2, 3, 4, 5, 12 and 13 have been found, and approximately 1 kb of sequence upstream of the putative start codon has been determined. In addition to the genomic sequence that was isolated, 5' RACE using pufferfish heart RNA produced a 334 bp cDNA sequence encompassing exons 2 to 5 of pufferfish PEA3. A pufferfish homologue of the human RNA helicase 1 (HRH1) gene was also found 3' of the PEA3 gene. Given that HRH1 is also found 3' of human PEA3 (E1AF) on chromosome 17q21, this finding would seem to indicate that chromosomal synteny is maintained between the human and pufferfish PEA3 loci. A four-way alignment of the mouse, human, chicken and pufferfish PEA3 promoters revealed that a region spanning from +1 to -260, relative to the transcriptional start site of mouse PEA3, is well conserved across the four promoters. Conserved transcription factor binding sites for SRY, HNF3β, NFY, AP-1, TCF, AP-2, v-myb, δEF1, and c-Ets-1 were found in three, and in some cases four of the promoters. An additional outcome of the pufferfish genomic library screen was the isolation of a pufferfish orthologue of the Ets transcription factor ERM. The relevance of these findings to the issue of transcriptional regulation of PEA3 expression is discussed. / Thesis / Master of Science (MS)

Thesis / Master of Science (MS)

The ability of simple oligopeptide complexes of nickel (II) to react with various dioxygen intermediates was investigated. Under physiological conditions, nickel (II)-histidine-containing oligopeptides were found to dismutate superoxide anions and disproportionate hydrogen peroxide. In the latter process, chemiluminescence was generated and a strongly oxidizing intermediate was detected capable of oxidizing uric acid, hydroxylating p-nitrophenol, and damaging 2-deoxy-D-ribose. The generation of this reactive intermediate likely occurs without the involvement of free hydroxyl radicals derived from Haber-Weiss or Fenton-type reactions. In addition, the Ni(II) complex of glycylglycyl-L-histidine (GGH) was found to react with mollecular oxygen resulting in the oxidation of the ligand. An attempt was made to relate these reactions to the involvement: of the nickel(III)/(II) redox couple which was shown to exist under physiological conditions. Similar reactivity was observed for non-histidine-containing oligopeptides but higher pH values were required. The oligopeptides used not only represent biologically relevant ligands but: the histidine containing oligopeptides mimics the specific copper(II)/nickel(II) binding and transport site of human serum albumin. The observations made in this study suggest some novel mechanism for the deleterious effects associated with excessive lifelong exposure to nickel compounds, especially in relation to cancer of the respiratory tract. / Thesis / Master of Science (MS)

No / Abstract: This paper is to apply the species conserving genetic algorithm (SCGA) to search multiple solutions of truss topology optimization problems in a single run. A real-vector is used to represent the corresponding cross-sectional areas and a member is thought to be existent if its area is bigger than a critical area. A finite element analysis model has been developed to deal with more practical considerations in modeling, such as existences of members, kinematic stability analysis and the computation of stresses and displacements. Cross-sectional areas and node connections are taken as decision variables and optimized simultaneously to minimize the total weight of trusses. Numerical results demonstrate that some truss topology optimization examples have many global and local solutions and different topologies can be found by using the proposed algorithm in a single run and some trusses have smaller weight than the solutions in the literature.

Cavity-nesting guilds are diverse communities of organisms that are hierarchically structured around the creation of and competition for tree holes as nest sites. Quantifying these communities as networks of interconnected species, or "nest webs", is a proven and efficient method for identifying important species and interactions within a cavity-nesting guild that can aid the management of these often threatened communities. Yet nest web data does not provide information regarding how natural resource managers are to encourage the support and adoption of conservation behaviors. This dissertation comprises two broad studies: one describing a cavity-nesting guild in central Namibia, and the other exploring methodology for identifying an effective flagship species for local conservation. In chapter 1, I provide background information of my study system and a review of the cavity-nesting and flagship species literature. In chapter 2, I quantify the species and resources within a cavity-nesting guild and create a cavity nest web to depict cavity creation and use in central Namibia. All cavity-nesters depended on sparse, large trees, which likely contributed to limited use of tree species across the guild. Cavity-excavators were of disparate importance for secondary cavity-nesters: important for small- and medium-bodied species but mostly unimportant for large-bodied species who depend more on large trunk hollows. In chapter 3, I describe and compare species-specific cavity resource niches among guild members to explore potential instances of cavity resource partitioning. Use of nest cavities appears to sort out by size among both cavity-excavators and secondary cavity-nesters. Excavators appear to partition cavity substrate spatially by decay class, while spatial partitioning among secondary nesters was largely by cavity type. Most interesting is the apparent temporal partitioning of large excavations among medium- and large-bodied cavity-nesters. In chapter 4, I explore a novel approach to flagship species selection that identified all previously-existing traits for flagship species, numerous traits specific to culture and personal experience, and a dozen new traits that were previously not recognized as potentially important for flagship species. This method shows considerable promise as a tool for investigating preferences for wildlife among broad stakeholders, as well as for engaging local people in wildlife conservation. / Doctor of Philosophy / Holes in trees, or cavities, are important resources for many birds, who use cavities as a safe place for raising young. Cavity-nesting birds are linked together in a cavity "nest web" based on the types of cavities used by each species. Understanding how birds are linked with each other and with certain types of cavities is critical in order to design conservation plans that preserve important community relationships. However, successful conservation also depends on strong support from local people, who are most often the people tasked with implementing the conservation actions. Flagship species can be used to gain support from necessary groups of people for specific conservation missions. This dissertation consists of two broad studies: the first study describes a community of cavity-nesting birds in Namibia, and the second explores a new way to investigate how people connect with wildlife through emotional connections. In chapter 1, I provide background information on my area of research. In chapter 2, I outline the different trees, cavities, and birds at the study site and estimate their abundance in the area. I also create a cavity nest web that illustrates the links between species. Large trees were relatively uncommon, but were important for all birds in the community. Cavities made by other birds, such as woodpeckers, were very important for small- and medium-sized species, but were mostly unimportant for large-sized species. In chapter 3, I compare the tree cavities used by different species, including the types of trees, cavities, and cavity sizes. I also explore multiple ways by which cavity-nesting birds might be dividing cavities between different species. Our most interesting finding was how medium-sized species seem to share cavities in time rather than in space; some species (i.e., small owls and woodpeckers) breed before some larger species that use the same type of cavity. Breeding early may be a way for some species to avoid competition with more competitive species, and may be the first example of this strategy being used by multiple cavity-nesters in a community. In chapter 4, I explore a new method for selecting flagship species. This method uncovered all traits that have been previously recommended for flagship species, as well as many traits specific to culture and personal experience. We also identified a dozen new traits that were previously not considered to be important for flagship species. Our new method is very promising as a tool for investigating preferences for wildlife among broad stakeholders, and for engaging and involving local people in wildlife conservation.

Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is a species of wood boring beetle which feeds on the inner bark of ash trees, Fraxinus spp., and white fringe tree, Chionanthus virginicus L. In North America emerald ash borer feeding damages the trees vascular system, killing the tree in 1-6 years after initial infestation. Emerald ash borer's native range is north east China, the Russian Far East, and the Korean peninsula. In the mid-1990s emerald ash borer was accidentally introduced to Michigan from the Hebei and Tianjin city province regions of China. Since then, due to human-aided transport and natural spread, emerald ash borer now infests 35 states and five Canadian provinces. Studies in to Asia discovered several species of parasitic wasps which feed and reproduce on emerald ash borer; four of these species were approved for release in the United States. Three species which attack emerald ash borer larvae Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae), Spathius agrili Yang (Hymenoptera: Braconidae), and Spathius galinae Belokobylskij and Strazanac (Hymenoptera: Braconidae), and one species which utilizes emerald ash borer eggs, Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae). This research focuses on the control of emerald ash borer using the three larvae-utilizing species of parasitic wasp. The first objective of this research focuses on the synchrony of emerald ash borer larvae with the early spring emergence of S. agrili and S. galinae. The first objective also assessed how two different cold temperature treatments changed the emergence timing, and health of the wasps. The second objective assessed to determine the effects of competition between S. agrili and S. galinae when they were exposed to a single emerald ash borer larvae sequentially, and simultaneously. When exposed sequentially, the first wasp held the competitive advantage, but when exposed simultaneously S. agrili had a small advantage, but did not completely exclude S. galinae. The third objective focused on the potential for two larval parasitoids, S. agrili and S. galinae to parasitize emerald ash borer larvae in the novel host plant white fringe tree. We determined that both parasitoids are capable of finding and parasitizing emerald ash borer larvae within a non-ash host. Finally, we located 13 stands of emerald ash borer infested ash in Virginia and North Carolina in order to determine its life cycle, and overwintering life stage. We determined that emerald ash borer overwinters at different life stages, and has a more complex life cycle than previously reported. Additionally, all three species of larvae-utilizing parasitic wasp were recovered at field sites at least 1 year after release. These results all show promise for the biocontrol program, and indicate that biocontrol of emerald ash borer could be successful in Virginia and North Carolina. / Doctor of Philosophy / Emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is a wood boring beetle which feeds on the inner bark of ash trees, Fraxinus spp., and white fringe tree, Chionanthus virginicus L. In the mid-1990s emerald ash borer was accidentally introduced to Michigan from the Hebei and Tianjin city province regions of China. Since then, due to human-aided transport and natural spread, emerald ash borer now infests urban and natural forests in 35 states and five Canadian provinces. North American ash trees did not evolve with emerad ash borer, and have little resistance to their attack. A North American ash or white fringe tree attacked by emerald ash borer dies in 1-6 years if left untreated. Systemic insecticide treatments exist, but require annual treatment and are less effective than initially hoped. Additionally, systemic insecticides are impractical to apply to forest ecosystems. Researchers traveled to the Asia, and discovered several species of parasitic wasps which attack emerald ash borer. After years of efficacy and host-exclusivity testing four species were released in the United States. Three species which exclusively utilize emerald ash borer larvae Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae), Spathius agrili Yang (Hymenoptera: Braconidae), and Spathius galinae Belokobylskij and Strazanac (Hymenoptera: Braconidae), and one species which utilizes emerald ash borer eggs, Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae). My research focuses on the control of emerald ash borer using the three larvae-utilizing species of parasitic wasp. Chapter two focuses on the life cycle of emerald ash borer larvae, S. agrili and S. galinae during the early spring. We determined that both parasitoid species have similarly timed life cycles as EAB, indicating a good climate match in Virginia. Chapter three describes the interactions between S. agrili and S. galinae when competing for EAB larvae. We determined that the first species to attack the larvae has the advantage; and when adult wasps competed to find a larvae neither species completely dominated the other. The third objective focused on the potential for two larval parasitoids, S. agrili and S. galinae to parasitize emerald ash borer larvae in the novel host plant white fringe tree. We determined that both parasitoids are capable of finding and parasitizing emerald ash borer larvae within white fringe tree. Finally, we located 13 stands of emerald ash borer-infested ash in Virginia and North Carolina in order to determine its life cycle, and overwintering life stage. We determined that emerald ash borer overwinters at different life stages, and has a more complex life cycle than previously reported. Additionally, all three species of larvae-utilizing parasitic wasp were recovered at field sites at least 1 year after release. These results all show promise for the biocontrol program, and indicate that biocontrol of emerald ash borer could be successful in Virginia and North Carolina.

Hepatitis E virus (HEV) is an important human pathogen, with pigs and likely other animal species serving as natural reservoirs. There are currently four recognized HEV genotypes that infect humans within the genus Hepevirus of the family Hepeviridae. Genotypes 1 and 2 are human viruses that are associated with waterborne and fecal-oral transmission in developing countries, while genotypes 3 and 4 have been identified in humans and other animal species and are zoonotic and endemic in both industrialized and developing countries. In my dissertation research, we identified the first strain of HEV from rabbits in the United States. We subsequently determined the complete genome sequence of the virus. Phylogenetic analyses of the full-length sequence indicated that U.S. rabbit HEV is a distant member of the zoonotic genotype 3, thus raising a potential concern for zoonotic infection. In order to investigate the cross-species potential of rabbit HEV, we then determined its antigenic cross-reactivity with other animal strains of HEV. Additionally, we demonstrated that the novel rabbit HEV could cross species barriers and infect pigs under experimental conditions. Finally, we attempted to determine the risk factors and sources of foodborne HEV infection in the United States. We detected HEV for the first time from non-liver pork commercial products in the United States and demonstrated consumption of undercooked meat a risk factor for HEV infection. HEV sequences of genotype 3 origin were detected from pork products purchased from grocery stores in Southwest Virginia. Approximately 6.3% (21/335) of university students tested seropositive for HEV antibodies and, importantly, those with a history of consuming undercooked meats were 13 times more likely to be seropositive. These results further underscore the importance of cooking pork thoroughly and using proper hygiene when preparing meals. / Ph. D.