Increased nutrient availability globally has the potential to affect community functional composition of plants in nutrient limited environments, such as coastal grassland systems. Stability of these systems are threatened worldwide by urbanization, as well as effects of sea level rise and increased frequency and intensity of storms, and atmospheric N deposition, associated with climate change. Annual net primary productivity (ANPP), species composition, and functional traits (community weighted specific leaf area (CWSLA), leaf area index (LAI), growth form and photosynthetic pathway) were measured across four treatments to assess multiple resource limitation of nitrogen (N) and phosphorus (P) and functional community response in a coastal grassland on Hog Island, VA within the Virginia Coast Reserve, Long Term Ecological Research Network (LTER) applied at a rate of 10 g m-2 yr-1 Nutrient enrichment did not alter species diversity or richness. ANPP was highest in plots receiving any type of nitrogen enrichment, and was higher than expected of low nutrient systems. CWSLA was significantly higher in NP plots, and was lower than other grasslands. P treatments were not significantly different from controls. Graminoid species, specifically C4 species responded with higher ANPP than C3 forbs or graminoids within treatments. Evidence of synergistic NP effects were seen on community level resource allocation and leaf construction, but no significant species changes occurred over a 1-year time span. These results have expanded the knowledge of functional response to increased nutrient availability in an understudied, coastal grassland, which are at high risk to being lost to sea level rise and anthropogenic development and inform community assembly processes in stressful environments.

Native to Europe and Asia, Lythrum salicaria is an invasive element on some other continents. Plants in the invaded areas are more robust, higher, and generally more successful compared to individuals in their original area. Based on my results these characteristics cannot be explained by neither higher photosynthesis, chlorophyll and carotenoid mass, nor specific leaf area.

This study examines the role of deffensive host-plant traits in structuring the community of leaf-chewing insects living on willows. Host-plant phylogeny was reconstructed and leaf morphology and content of three different groups of secondary metabolites were measured. Relationships between defensive leaf traits were examined and their influence on insect community structure was analyzed.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / This study was conducted in the city of Restinga Sêca, RS, with the objective to analyze the productivity, quality, botanical composition and biological characteristics of native pasture in response to fertilization and water availability. The fertilization increased native pastures productivity. The dry matter total accumulation rate, at dry or wet area, both fertilized, was 102 kg/ha/day. In the same areas, not fertilized, was 47 and 41 kg/ha/day, respectively. For green matter, the fertilization also increased the productivity, reaching accumulation rates of 70 and 93 kg/ha/day for dry and wet areas, respectively. In these not fertilized areas, the rates were 29 and 25 kg/ha/day. Furthermore, increased nitrogen and crude protein rates in plant tissues and mineral matter quantity in the plots. Water availability influenced on pasture components, as the water availability was increased, lower amount of components such as strait leaf, broad leaf and legumes and higher amount of neutral detergent fiber in tissues were observed, especially in spring period. Fertilization as well as water availability decreased the wealth of species, causing domination of some species already adapted to these conditions. Two functional groups were formed with vegetal biological characteristics, a group of capture of resources, with a high specific leaf area (SLA) (26) and low dry matter content (DMC) (234), composed by Paspalum pumilum, Paspalum urvillei, Paspalum pauciciliatum, Axonopus affinis and Setaria glauca. The other group is the conservation of resources, low SLA (21) and high DMC (323), composed by Paspalum notatum and Andropogon lateralis. / O presente trabalho foi realizado em área de pastagem natural no município de Restinga Sêca, RS, e teve como objetivos analisar a produtividade, qualidade, composição botânica e os atributos biológicos vegetais da pastagem natural em resposta à adubação e à disponibilidade hídrica. A adubação aumentou a produtividade da pastagem nativa. A taxa de acúmulo de MS Total tanto na área seca como na úmida, ambas adubadas foi de 102 kg/ha/dia. Nas mesmas áreas, não-adubadas, foi de 47 e 41 kg/ha/dia, respectivamente. Com relação ao material verde, a adubação também aumentou a produtividade chegando a taxas de acúmulo diária de 70 e 93 kg/ha para área seca e úmida, respectivamente, nessas áreas não-adubadas, as taxas foram de 29 e 25 kg/ha/dia. Além disso, aumentou o índice de nitrogênio e de PB nos tecidos vegetais e a quantidade de MM nas parcelas. A disponibilidade hídrica influenciou nos componentes da pastagem, quanto maior a disponibilidade de água, menor a quantidade de componentes como folha estreita, folha larga e leguminosas e maior a quantidade de FDN nos tecidos, principalmente no período de primavera. Tanto a adubação como a maior disponibilidade hídrica diminuíram a riqueza de espécies, fazendo com que algumas espécies adaptadas a essas condições dominassem a comunidade. Formaram-se dois grupos funcionais a partir dos atributos biológicos vegetais, um grupo de captura de recursos, com alta AFE (26) e baixo TMS (234), onde fazem parte as espécies Paspalum pumilum, Paspalum urvillei, Paspalum pauciciliatum, Axonopus affinis e Setaria glauca. O outro grupo é de conservação de recursos, com baixa AFE (21) e alto TMS (323), onde Paspalum notatum e Andropogon lateralis foram as espécies agrupadas.

Native to Europe and Asia, Lythrum salicaria is an invasive element on some other continents. Plants in the invaded areas are more robust, higher, and generally more successful compared to individuals in their original area. Based on my results these characteristics cannot be explained by neither higher photosynthesis, chlorophyll and carotenoid mass, nor specific leaf area.

Linking environmental variation to population dynamics is necessary to understand and predict how the environment influences species abundances and distributions. I used demographic, environmental and trait data of forest herbs to study effects of spatial variation in environmental factors on populations as well as environmental change in terms of effects of forest succession on field layer plants. The results show that abundances of field layer species during forest succession are correlated with their functional traits; species with high specific leaf area increased more in abundance. I also found that soil nutrients affect vegetative and flowering phenology of the forest herb Actaea spicata. The effect of nutrients shows that a wider range of environmental factors than usually assumed can influence plant phenology. Moreover, local environmental factors affected also the demography of A. spicata through effects on vital rates. An abiotic factor, soil potassium affecting individual growth rate, was more important for population growth rate than seed predation, the most conspicuous biotic interaction in this system. Density independent changes in soil potassium during forest succession, and to a lesser extent plant population size dependent seed predation, were predicted to alter population growth rate, and thereby the abundance, of A. spicata over time. Because these environmental factors had effects on population projections, they can potentially influence the occupancy pattern of this species along successional gradients. I conclude that including deterministic, as opposed to stochastic, environmental change in demographic models enables assessments of the effects of processes such as succession, altered land-use, and climate change on population dynamics. Models explicitly incorporating environmental factors are useful for studying population dynamics in a realistic context, and to guide management of threatened species in changing environments.

Grasses are widely used in revegetation to control erosion, build soil and maintain habitat. In northeast British Columbia, non-native grass species are commonly seeded to reclaim industrially disturbed sites. Widespread concern about degradation of biodiversity and key ecological processes has led to increasing value placed on native species and management practices leading to a more resilient landscape. I undertook this study to fill the restoration knowledge gap relating to native grasses in northeast BC. I did an extensive inventory of grasses on 217 sites in 2007, 2008 and 2009. Functional traits were measured in the field and in a greenhouse growth experiment. I found ninety-nine grass species occuring in the region, 70% of which are native. The number, proportion and extent of non-native grasses are increasing and four of these – Poa pratensis, Festuca rubra, Bromus inermis, and Phleum pratense represented almost a quarter of all occurrences. Several native species were common throughout the region: Calamagrostis canadensis, Leymus innovatus, Elymus trachycaulus, Poa palustris and Agrostis scabra. Other native species, including Festuca altaica, Koeleria macrantha, Pascopyrum smithii, and Schizachne purpurascens, strongly favoured intact habitats. Elevation, soil moisture regime, proportion of bare ground, and land use were significant factors related to local grass species composition and abundance. Agrostis scabra, Alopecurus aequalis, Beckmannia syzigachne, Bromus ciliatus, Cinna latifolia, Deschampsia cespitosa, Elymus alaskanus, Elymus trachycaulus, Festuca saximontana and Hordeum jubatum grew commonly on severely damaged well sites. Field measurements for Specific Leaf Area (SLA) and Leaf Dry Matter Content (LDMC) of 11 species showed an inverse correlation. Bromus ciliatus, Bromus pumpellianus, and Elymus trachycaulus had high SLA/low LDMC linked to rapid growth, whereas Festuca altaica, Deschampsia cespitosa, and Calamagrostis stricta had low SLA/high LDMC linked to slow growth and persistence. In the greenhouse experiment, Poa palustris, Cinna latifolia and Bromus ciliatus produced the most overall biomass and Pascopyrum smithii and Poa palustris produced the greatest aboveground biomass. Calamagrostis stricta, Poa palustris, Elymus glaucus, Leymus innovatus and Pascopyrum smithii exhibited clonal growth. Beckmannia syzigachne, Bromus ciliatus Cinna latifolia produced viable seed during the 135-day experiment. Considering all attributes five native species, Calamagrostis canadensis, Elymus trachycaulus, Poa palustris, Leymus innovatus, and Agrostis scabra are recommended for general restoration use in northeast B.C. Other native species show promise when matched to particular site conditions, including Alopecurus aequalis, Arctagrostis latifolia, Beckmannia syzigachne, Bromus ciliatus, Calamagrostis stricta, Cinna latifolia, Deschampsia cespitosa, Elymus glaucus, Festuca saximontana, Glyceria striata, Hordeum jubatum, Koeleria macrantha, Pascopyrum smithii, Poa alpina, Schizachne purpurascens and Trisetum spicatum. This information will be valuable to land managers interested in moving beyond reclamation to ecological restoration of sites disturbed by oil and gas development. Developing practices that are environmentally sound and socially acceptable requires ongoing botanical inventory. Plant traits may be useful in matching species to site conditions and restoration goals. Policy recommendations include phasing in of requirements to use native seed while restricting the use of agronomic species, promoting natural colonization, and supporting a native seed industry.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Plant functional types defined from morphological attributes are fundamental to understanding functioning of grasslands communities. Thus, to build a classification based solely on grasses (family of greater biomass contribution), it is necessary for increasing the knowledge about complex ecosystems. This work aims to characterize vegetation dynamics by species and by functional types of grasses of two RS physiographic regions (Depressão Central-Santa Maria and Campanha-Bagé) under different managements. The evaluated treatments were: natural pasture and natural pasture overseeded with cool season species (Bagé) and natural pasture submitted to burning and grazing treatments (Santa Maria). For this, permanent transects were used in order to represent the different communities at paddock level. Vegetation dynamics was evaluated using procedures of BOTANAL method. Tillers of grasses with contributions exceeding 3% of total aboveground biomass were collected for subsequent measurement of the attributes specific leaf area (AFE) and leaves dry matter content (TMS). Burned treatments presented greater contribution of Andropogon lateralis, while in grazed treatments there was a greater species diversity. Introduction of cool season species combined with fertilizer promotes an increase of species characterized by resources´ capture. TMS was more stable for species linked to capturing resources strategy and AFE was more robust for species characterized by resources conservation. Therefore, until a regional data basis were developed, it is recommended to use both attributes for future research. / Tipos funcionais de plantas definidos a partir de atributos morfológicos são fundamentais para o entendimento do funcionamento de comunidades campestres. Desta maneira, a construção de uma tipologia baseada apenas em gramíneas (família de maior contribuição), torna-se necessária para o avanço do conhecimento sobre ecossistemas complexos. Este trabalho tem por objetivo caracterizar duas regiões fisiográficas do RS (Depressão Central-Santa Maria e Campanha-Bagé) sob distintos manejos quanto à dinâmica vegetacional por espécies e por tipos funcionais de gramíneas. Os tratamentos avaliados foram: pastagem natural e pastagem natural com introdução de espécies hibernais (Bagé) e pastagem natural submetida a tratamentos de queima e pastejo (Santa Maria). Para isto, foram dispostas transectas fixas de modo a representar as diferentes comunidades em nível de potreiro. Foi avaliada a dinâmica vegetacional utilizando os procedimentos do método BOTANAL. Foram coletados afilhos das gramíneas com contribuição superior a 3% da biomassa aérea total para posterior medida dos atributos área foliar específica (AFE) e teor de matéria seca da folha (TMS). Os tratamentos queimados tiveram maior contribuição de Andropogon lateralis, enquanto nos tratamentos pastejados houve uma maior diversidade de espécies. A introdução de espécies de estação fria, aliada à adubação, promove um acréscimo das espécies caracterizadas por captura de recursos. O TMS se mostrou mais estável para espécies ligadas à estratégia de captura de recursos e a AFE mais robusta para as espécies caracterizadas pela conservação de recursos. Por tanto, até que se desenvolva uma base regional de dados, recomenda-se que futuras pesquisas continuem a utilizar os dois atributos.

Plant functional traits (plant height, aboveground biomass, R/S ratio, specific leaf area, leaf dry matter content, specific root length and arbuscular mycorrhizal infection) were measured for several grassland plant species growing under different soil fertility conditions in a greenhouse and a field experiments. Traits` response to nutrient addition and the differences in traits among species and between forbs and grasses were studied. Results from the greenhouse and the field experiment were compared.