The mammalian target of rapamycin (mTOR) complex is a key regulator of protein synthesis, with resistance exercise and protein ingestion both shown to increase mTOR activity in human skeletal muscle. It has recently been proposed that mTOR activity is regulated via its intracellular localization and protein complex interaction. However, no research to date has examined this process in human skeletal muscle. Accordingly, the aims of this thesis were to (1) develop immunofluorescent-based methodologies to study mTOR in human skeletal muscle, and (2) apply this approach to the study of mTOR in acute and chronic resistance exercise scenarios. This thesis describes a novel approach to study mTOR regulation in human skeletal muscle in vivo. Taking advantage of this approach, novel data was presented on mTOR distribution, translocation and association with regulators in response to resistance exercise in human skeletal muscle in vivo. It is hoped that this approach will provide insight into the cellular regulation of skeletal muscle protein synthesis and by extension the control of skeletal muscle mass in humans during scenarios of health and chronic disease.

Taking part in regular physical activity leads to adaptive response that enables the body’s antioxidant defence to be better equipped to fight against oxidative stress. Exercise intensity seems to be one of key factors that determines the effectiveness of exercise. The work presented in this thesis used novel approaches, through the application of emerging technologies, to study physical activity and its effects. This thesis contributes to the existing literature by being the first to investigate the effect of exercise on a marker of oxidative stress in the brain, an organ that becomes impaired (including oxidative damage) with ageing and diseases associated with ageing. The finding from this thesis suggests that brain glutathione (GSH) of young sedentary men as measured by magnetic resonance spectroscopy (MRS) was altered in response to acute exercise, in an exercise intensity dependent manner. Observed changes in peripheral markers of oxidative stress were also exercise intensity dependent. The brain seems to be protected against hyperperfusion injury during high intensity phase of high intensity interval exercise. Objectively measured physical activity levels were not significantly increased by an unsupervised home-based exercise intervention in older adults, potentially due to a lack of progressive goals based on adherence to physical activity.

The thesis outlines a collaborative research project that was undertaken between the Malta Football Association (MFA), the Malta National Sports School (NSS) and Liverpool John Moores University (LJMU). Drawing on transition frameworks, models and contemporary literature (Schlossberg, 1981; Stambulova 2003; Wylleman & Lavalle, 2004; Morris, Tod & Oliver, 2015; Morris, Tod & Eubank, 2016; Richardson, Littlewood & Gilbourne, 2005; Richardson, Littlewood, Nesti & Benstead, 2012), the research adopted an action research methodology to examine the psycho-social and cultural challenges that young Maltese football players and parents experienced within migratory based transitions. The author adopted the role of practitioner-researcher to understand, plan and support a range of key stakeholders during the transition process. Specifically, Study One (Reconnaissance Phase) examined the challenges experienced by Maltese footballers that had migrated to European professional football. Individual semi-structured interviews were conducted with 12 male Maltese players who had experienced migration to a foreign club. Results evidenced that players experienced homesickness and lacked psycho-social knowledge and skills for an effective transition. Players also experienced culturally based challenges. The findings are framed around a unique and deeply embedded Maltese cultural identity. Study Two (Reconnaissance Phase) utilised a focus group methodology with parents of players from the Maltese Football Association (MFA) Academy, parents of young players that had previously experienced migration, head coaches from top Maltese nurseries, and coaches from the National Sports School (NSS) and MFA. Findings of Study One were disseminated to facilitate reflection, discussion and to identify issues that required consideration in order to better prepare young players for future migratory based transition. Study Three (Action Planning) used focus groups to disseminate the findings of Study One and Two with the Headmaster of the NSS, and parent representatives of the school. A number of change strategies were developed to improve the school’s programme of preparation for students who may migrate to foreign clubs. A separate action meeting was conducted with the Technical Director of the MFA to discuss and highlight change strategies to improve the MFA’s programme of preparation for young footballers seeking a career abroad in professional football. The Implementation and Monitoring phases focused on applying the action strategies agreed. These were; (i) to experience migration and independent living, (ii) psycho-social and cultural support and development of young players, and (iii) parental education. Regular evaluation of change strategy activities evidenced positive change and also continued to enhance the efficacy of the strategies being implemented. Young players who have migrated overseas over the past year and who had engaged in a programme of preparation prior to migration have coped well with the challenges they have so far faced.

Alzheimer’s disease (AD) is a devastating neurodegenerative disorder which is thought to affect 26.6 million individuals worldwide. There is growing concern over a worldwide dementia epidemic that is predicted to develop over the coming decades. The evidence thus far suggests that increased levels of oxidative stress and vascular risk factors are two major contributors, amongst others, to AD development. The thesis aimed to investigate markers of oxidative stress in AD plasma. Moreover, the oxidative status of specific proteins was investigated using both hypothesis driven and proteomic approaches. Results presented in this thesis suggest that global plasma protein oxidation levels are not different when AD and control subjects are compared, but that individual plasma proteins are specific targets for oxidative modification in AD. The thesis explores different methodologies to assess oxidative changes in AD. In addition it demonstrates that emerging novel and powerful mass spectrometry techniques can be employed successfully to identify several proteins modified by oxidation, providing an initial starting point for further investigation.

Climbing stairs is a lifestyle physical activity with effects on a range of CVD risk factors. This thesis explored the potential of stair climbing at home as a public health intervention in three empirical studies. A pilot study tested the feasibility of progressive increases in stair climbing and descent at home. Eight participants progressed from 10 floors.day-1 in week one to 19 floors.day-1 in week four. Percent body fat, SBP and leg power were improved at the end of the brief intervention. The second study randomly allocated 24 healthy weight (BMI = 22.1) and 26 overweight (BMI = 31.7) sedentary women to gym-based and home-based stair climbing for 5 days.week-1 over 8 weeks, with a healthy weight control group recruited for comparison. Intervention participants progressed from 2 continuous 32.8m ascents.day-1 in weeks 1-2 to 5 ascents.day-1 in weeks 7-8. Stair climbing improved body composition, cardio-respiratory fitness and serum lipid profiles. Increases in leg power were found in the stair climbing group. Overall, effects were similar for gym-based and home-based interventions. Given the effects of leg power, and the importance of leg power in the elderly, the third study investigated the potential psychological determinants of increased stair climbing at home for an older population. Participants (n = 281; age = 69.2 years) reported the number of floors that they were willing to climb continuously at home, as well as potential barriers and facilitators of the behaviour. Positive beliefs about the benefits of regular stair climbing and fear of falling, both on stairs and in general, were the major predictors of willingness to climb stairs at home. Discussion focuses on the potential of home-based stair climbing as a cost-effective intervention for preservation of function and CVD risk in public health.

Within Exercise Science, there are ongoing debates among health and fitness professionals over exercise related health questions. It can be difficult to discern myth from science when there are poorly designed research studies or limited evidence to unequivocally answer the question. Therefore, the purpose of this symposium is-to have a panel of experts provide research-based evidence related to three controversial topics often posed to exercise science professionals. First, within the weight loss community there is frequent debate over the role of exercising in the fat burning zone for weight loss. Dr. Ed Howley will address the questions: what is the fat burning zone, and what is its significance for those trying to lose fat? Secondly, there has been an interest in the concept of a runner's high and some early research linked this phenomenon to brain endorphins. Dr. Pat O'Connor will address the questions: is there a physiological basis for euphoria associated with exercise and what role do endorphins play in this phenomenon? Lastly, the health related benefits of cardiovascular and resistance training are well documented, but the importance of stretching for health and sports performance is less clear. Dr. Mike Stone will address the questions: does research support the role of stretching in enhanced health and athletic performance and are there situations in which stretching might be considered contraindicative?

The participation of children and early and late adolescents in resistance training activities, including training for sport, appears to continually be on the rise. The involvement of youth in the sport of weightlifting and the use of weightlifting methods as part of training for sport performance also appears to be growing. The increased acceptance of these activities is evidenced by the number of both lay and scientific articles, specialized magazines and publications devoted to the different types of resistance training. It is also indicated by simple observation of fitness centres and sports training facilities. The use of resistance training appears to be growing in popularity despite poorly supported position statements, anecdotal reports and speculation concerning the potential for injury. Children and adolescents participating in resistance training programs can make noticeable increases in strength and power as well as physiological parameters associated with both health and sport performance. Although the number of children and adolescents (aged 8–18 years) involved in some form of resistance training is increasing and evidenced-based reviews that support these activities have been published, controversy still exists. There are still those who insist that training with free weights, particularly the sport of weightlifting, should not take place until a person is fully developed, even though there is not much objective evidence to support such a position. More recently published scientific data support the notion that properly supervised resistance training programs can improve sport performance, reduce injury potential and enhance healthy facets of children and early and late adolescents.

No description available.

The use of complementary and alternative medicines and therapies (CAM) in Australia and across the world is becoming increasingly prevalent. The most recent survey conducted by MacLennon et al. in 2004 [2] identified that more than half the general Australian population had used some form of CAM in the previous 12 months before the survey was conducted.[2-4] Up until now, the prevalence of CAM use among elite Australian athletes was unknown. As athletes are constantly looking to gain an advantage over their competitors through physical or psychological interventions or through the application of new technologies, it was hypothesised that the use of CAM in this group would be higher in athletes than in the general population. After surveying 497 elite Australian athletes we found that 93% of this specific population utilised at least one CAM within their lifetime. The 10 CAM (as defined by the Therapeutics Goods Administration) used most frequently were sports drinks, massage, sports bars, cereal bars, multivitamins, aloe vera, sports gels, Pilates, caffeine and yoga. There were no significant differences identified between specific sporting groups- football codes, court sports, field sports, water sports and others, with all groups reporting high usage. Sports physicians, family, coaches and friends were identified as the main sources for product information, with health food shops, sports dieticians and sports physicians being the predominant providers of the actual product. Four CAM sold on the Australian market which purported to decrease the symptoms of delayed onset muscle soreness (DOMS), or reported anti-inflammatory properties which could impact of the inflammatory response associated with DOMS were studied. Lyprinol® (an anti-inflammatory agent), topical Arnica (for symptomatic relief of soft tissue trauma), Tienchi Ginseng (proposed to relieve symptoms of DOMS) and Devil’s Claw (an analgesic and anti-inflammatory agent) were the chosen CAM. Four separate randomised, double blind placebo controlled studies comprising of 20 subjects per study were carried out with the same DOMS inducing methodology (downhill treadmill running) employed. All subjects in each of the four studies had their performance (counter movement and squat jump, maximal force), pain (visual analogue scale and muscle tenderness) and blood parameters (high sensitivity C-reactive protein, interleukin-1A, interleukin-6, tumour necrosis factor-K, creatine kinase and myoglobin) analysed seven times over five days. Lyprinol® did not affect performance, pain or blood markers of muscle damage and inflammation analysed in this study. Despite Lyprinol® being marketed as having “potent anti-inflammatory” properties, Lyprinol® did not demonstrate any antiinflammatory properties in our sample group at a dose of 200mg daily for two months, and did not alter any of the markers of inflammation after a downhill running protocol. A significant difference was identified in quadriceps muscle tenderness between the topical Arnica and placebo groups 72 hours after the downhill running protocol. This indicated that the topical Arnica group experienced less quadriceps pain at this time point, though this was not reflected in the quadriceps visual analogue scale results. There were significant differences identified between the topical Arnica and placebo groups for muscle tenderness in the gastrocnemius and tumour necrosis factor-K concentration at baseline. Further statistical analysis assessing relative changes from baseline did not demonstrate any statistically significant differences between the groups for either of these parameters. We therefore conclude that the symptomatic relief of soft tissue trauma claimed from a topical Arnica product sold in Australia was not conclusively demonstrated in this study. The single, isolated significant difference identified in quadriceps tenderness does not conclusively, from this study, indicate efficacy for the use of this topical Arnica for the relief of soft tissue trauma. Tienchi Ginseng demonstrated the most promising outcomes, with statistically significant differences identified in performance and inflammatory markers in favour of Tienchi Ginseng. Though it cannot be conclusively deemed beneficial for DOMS from this study alone, Tienchi Ginseng warrants further research with larger sample sizes and a similar muscle damage protocol. Finally, Devil’s Claw did not demonstrate beneficial outcomes in regards to DOMS within this study. It actually demonstrated some, perhaps detrimental effects, upon analysis of performance and inflammatory markers. We are unsure of the mechanisms behind these findings, particularly when considering the proposed anti-inflammatory effects of Devil’s Claw. From the literature, Devil’s Claw appears to be beneficial in chronic musculoskeletal conditions however it does not seem to impact on muscle damage and pain resulting from DOMS inducing exercise. The questionnaire study has demonstrated that there is a high usage of CAM among elite Australian athletes, an area up until now that has been unexplored. The onset of CAM use within the Australian athletic population, and the prevalence of CAM use by other professional Australian athletes would be an area to explore in the future. In regards to products which specifically claim to benefit athletes suffering soft tissue trauma such as DOMS, convincing evidence for specific product use was not demonstrated in these studies. Further research involving greater sample sizes may reveal more definitive outcomes, specifically in regards to the use of Tienchi Ginseng. / Doctor of Philosophy (PhD)

This autoethnographic study investigates the emotional and social dimensions of a coaching relationship from the athlete�s perspective. Autoethnography is an approach that draws on highly personalised biographical accounts in which authors tell stories about their lived experiences (Ellis & Bochner, 2000; Richardson, 2000) in order to place the "self within a social context" (Reed-Danahay, 1997, p. 9). Consequently, through the analysis of my memories and ethnographic notes, I analyse my experiences as an Olympic marathon runner and, in particular, the challenges I faced with my coach. In the process of investigating the emotional and social dimensions of the coaching process (which I have divided into three phases), I focus specifically on the creation of (1) my 'athletic identity', (2) the power relationship that developed between my coach and myself and, (3) my early retirement from running. In order to make sense of my experiences, I draw upon theories of identity (e.g. Bradley, 1996), Foucauldian concepts on 'power' (e.g. Foucault, 1980), and the literature addressing 'premature retirement' (e.g. Sparkes 1996; 2000). Finally, a conclusion summarises the main points made in addition to outlining their implications for further coaching research and practice.