Three populations of Southern flying squirrels were studied in the Ouachita Mountains of Arkansas to assess the impact of population subdivision-due to island formation--on the population genetics of Glaucomys volans. One island, one mainland, and one open population were investigated. A 367 nucleotide hypervariable region of mitochondrial DNA was sequenced in individuals from each population. Individuals and populations were compared to assess relatedness. Higher sequence diversity was detected in the open and island populations. One island individual shared characters with both the island and mainland populations. Results support the hypothesis that the mainland population may have reduced gene flow. Also, the island population may have been originally founded by at least two maternal lineages.

The thesis deals with vocalizations produced by Eurasian ground squirrels of the genus Spermophilus. It is mainly focused on inter-species and intra-species variability in acoustic structure of alarm calls which are emitted in presence of predators.The major part of the thesis deals with intra-species variability in acoustic structure of the alarm calls in five Eurasian ground squirrels, Spermophilus citellus, S. taurensis, S. xanthoprymnus, S. suslicus and S. fulvus. It has been confirmed that the alarm calls of these ground squirrels show a high level of species specificity. The thesis further describes another vocalizations produced by three Eurasian ground squirrel species, Spermophilus suslicus, S. citellus and S. fulvus, and shows that with the exception of the alarm calls, vocal repertoires of these three ground squirrels are remarkably similar. The last part of the thesis deals with individual distinctiveness of the alarm calls of three Eurasian ground squirrels, Spermophilus citellus, S. taurensis and S. xanthoprymnus. It has been shown that the alarm calls of these species have a significant potential to encode information about caller identity.

This research describes and analyzes the behavior of free-living Uinta ground squirrels (Spermophilus armatus) temporally, spatially and with reference to differences in sex, age, population density and habitat. In addition, the relationships between local meteorological parameters and time spent above ground are described and analyzed. The frequency of eight behaviors, feeding, moving, encounters, upright, motionless, grooming, nest gathering and calling, among ground squirrels was found to be labile, varying in frequency with time of day, period of the season, sex, age, population density and habitat, and varying in spatial distribution within the home range. The dominant behavior, feeding, was most frequent during the first and last two hours of the day and tended to occur within the home range core during these hours. During the midday periods home range use became more general and the frequency of the other behaviors increased as feeding frequency decreased. During periods of sexual activity (the beginning and end of the homeothermic season), male ground squirrels displayed high frequencies of encounters, moving and calling behaviors.

Habitat fragmentation and destruction caused by linear infrastructure, including roads, railways, and power line corridors, are recognized as major threats to biodiversity around the world. Roads can act as barriers by impeding animal movement and restricting animal space use. An understanding of factors that influence barrier effects is important to discern the impacts of habitat fragmentation and to develop appropriate mitigation. The barrier effects of roads are driven by several distinct but not mutually exclusive mechanisms that include traffic, edge, and gap avoidance. We used an endangered forest obligate, the Mount Graham red squirrel (Tamiasciurus hudsonicus grahamensis), as our study organism to assess effects of traffic noise on animal occurrence and demonstrated that traffic noise had spatially extensive and negative effects on site occupancy after accounting for effects of distance from roads and the environment. We investigated barrier effects of forest roads and assessed effects of traffic, road edges, and canopy gaps on space use of Mt. Graham red squirrels and compared to the response of introduced, edge-tolerant Abert's squirrels (Sciurus aberti). Forest roads acted as partial barriers for red squirrels regardless of traffic volume likely due to avoidance of canopy gap created by roads, whereas Abert's squirrels showed no avoidance of roads. Therefore, roads restricted movement and space use of a native forest-dependent species while creating habitat preferred by an introduced, edge-tolerated species. Through a meta-analysis of studies that quantified road crossing behavior by mammals, we found that all types of roads, from major highways to narrow forest roads, can impede movement for certain species of mammals. Magnitude of barrier effects of roads decreased as species body mass increased, and was affected positively by increasing road width. We suggest that the species-specific magnitude of barrier effects of roads may be anticipated with basic information from life history traits and road characteristics that are readily accessed through open resources or easily measured.

No description available.

Classically, testosterone (T) was considered the principal regulator of aggression. However, recent studies in birds have found aggression and T uncoupled during the non-breeding season. Circulating testosterone comes with costs such as immunosuppression and energy expenditure. Instead, the pro-hormone, dehydroepiandrosterone (DHEA), is circulated and activated within the brain via conversion into oestradiol (E2), avoiding the costs associated with T. At present the site of DHEA synthesis is unknown. My thesis investigated the existence of an analogous pathway in non-breeding red squirrels (Tamiasciurus hudsonicus) with two studies: (a) a field study investigating the effects of E2 on aggression and (b) a laboratory study that attempted to determine the site of DHEA synthesis. I conclude that E2 regulates non-breeding aggression in red squirrels and that the adrenals are not the site of DHEA synthesis. My results suggest the existence of a mammalian analogue to the regulatory pathway found in birds.

Classically, testosterone (T) was considered the principal regulator of aggression. However, recent studies in birds have found aggression and T uncoupled during the non-breeding season. Circulating testosterone comes with costs such as immunosuppression and energy expenditure. Instead, the pro-hormone, dehydroepiandrosterone (DHEA), is circulated and activated within the brain via conversion into oestradiol (E2), avoiding the costs associated with T. At present the site of DHEA synthesis is unknown. My thesis investigated the existence of an analogous pathway in non-breeding red squirrels (Tamiasciurus hudsonicus) with two studies: (a) a field study investigating the effects of E2 on aggression and (b) a laboratory study that attempted to determine the site of DHEA synthesis. I conclude that E2 regulates non-breeding aggression in red squirrels and that the adrenals are not the site of DHEA synthesis. My results suggest the existence of a mammalian analogue to the regulatory pathway found in birds.

Northern Goshawk breeding habitat was conceptualized and analyzed as a system composed of different sized components organized within a hierarchy. The organization of the hierarchy was asymmetric with smaller components nested within and subordinate to larger components. Components considered in the breeding habitat hierarchy of goshawks were nest tree, nest site, nest territory, foraging area, and landscape. Choice at any level within the hierarchy was contrasted with the next level in the hierarchy. Thus it was found that goshawks chose nest trees with a largerdbh (79.0 ± 4.4 cm) than the nest site average (43 ± 2.7 cm) (p=0.000036); the averagedbh of the nest site (43.2± 3.1 cm) was significantly larger than the territory (33.2± 1.9cm) (p=0.018). The nest territory when contrasted with random unoccupied locations in the landscape demonstrated a composition of a central site of trees with largedbh and fewer stems when compared with the surrounding forest. I hypothesize that this pattern reduces thepredation risk at the nest site. I investigated the nest site relative to the assumed foraging area (radius of 3000m; approximately 3000ha). Goshawks selected nest sites which were significantly further from young clear cuts (>10 ha). The average distance from a nest site to a recentclearcut greater than 10 ha was 1,350 m of the nest while for unoccupied sites the average distance was 770 m (p = 0.011). Finally, at the landscape level, areas of approximately 10,000 ha, goshawks on Vancouver Island were more abundant, had more nest sites, and had higher productivity per active nest in old growth forests (>120 years) than in either second growth (majority less than 80 years) or fragmented landscapes. The majority of goshawk prey, as determined though pellet analysis was red squirrel (69%). Red squirrels were equally abundant in second growth and old growth yet less abundant in fragmented landscapes. Despite similar abundance of their main prey item goshawks were not as abundant in second growth forests as in old growth forests. I conclude that the structure of second growth and fragmented landscape reduces the availability of key prey species and therefor the suitability of these landscape fpr goshawks is lower. / Graduate

Uinta ground squirrels (Spermophilus armatus) were instrumented with ECG radio-transmitters. Heart rate and oxygen consumption were monitored for one hour at ambient temperatures above, within and below the thermoneutral zone. These measures were made at predetermined intervals throughout the active season of squirrels to determine if the heart rate-oxygen consumption relationship was a stable linear regression within and between squirrels during this period. Heart rate and oxygen consumption were also monitored for squirrels exposed to artificial and natural stressors. These squirrels were then released in an outdoor enclosure. Heart rate and behavior of animals were monitored simultaneously during above and below ground activity. Heart rate-oxygen consumption regressions were stable during measurements through a 25°C temperature range. Regressions of heart rate versus oxygen consumption did vary between and within squirrels over four days. Heart rate tended to decrease initially after presentation of natural stressors. Heart rate tended to increase when artificial stressors were presented. Regressions of heart rate versus oxygen consumption for these stressors tended to be linear, but heart rate would not be a good predictor of oxygen consumption during rapid heart rate changes because, in most cases, heart rate-oxygen consumption regressions were not statistically significant. In some tests with rapidly decreasing initial heart rates, the heart rate-oxygen consumption relationship was negative. that is, oxygen consumption remained constant as heart rate decreased. Possible causes of negative responses are discussed. Heart rate responses generated in laboratory stress tests were similar to semi free-living heart rate responses. During the major portion of time spent above and below ground, heart rate of squirrels was found to be quite stable. During these periods, heart rate should be a reasonable predictor of oxygen consumption. In summary, average heart rate should be a reasonable predictor of oxygen consumption of free-living squirrels over daily periods. In using this method, it would be necessary to calculate regressions of heart rate and oxygen consumption in the laboratory before and after free-living studies to examine the stability of the regression lines during this period. An average regression line could be used to compensate in part for a change of the heart rate-oxygen consumption relationship during study periods.

Various methods were employed to detect changes in the peripheral degradation of thyroxine (T4) in adult Uinta ground squirrels (Citellus armatus) collected at two different altitudes (5400 and 9000 feet.) Plasma half-life of injected T4-125I (tl/2) was significantly decreased with altitude, as was T4-125I distribution space (TDS) and T4 degradation and secretion rate (TSR). In vitro deiodination of T4-125I by heart homogenates was not affected, while urinary excretion of thyroxine derived iodide was increased at high altitude 44-70 hours post-injection. Both total and free serum T4 concentrations were reduced at high altitude, although not significantly. The cause of the reduced tl/2 at high altitude was not evident, while the decreased TDS was probably due to a reduced plasma volume. Increased urinary and fecal clearance could account for the decreased total and free serum T4. TSR was reduced due to the low TDS and T4 concentration observed in high altitude squirrels.