<p>The main hypothesis in this study is the development of a nanocomposite mediated amperometric biosensor for detection of hydrogen peroxide. The aim is to combine the electrochemical properties of both polyaniline and ferrocenium hexafluorophosphate into highly conductive nano composites capable of exhibiting electrochemistry in non acidic media / shuttling electrons between HRP and GCE for biosensor applications.</p>

The main objective of this work was to develop simple and sensitive electrochemical sensors for the detection of bisphenol A. To investigate the electrochemical behavior of BPA on a bare glassy carbon electrode. To apply the developed biosensor for the determination BPA by differential pulse voltammetry, electrochemical impedance spectrometry, square wave voltammetry and steady-state amperometry. To characterize the synthesized PDMA-PSS by cyclic voltammetry (CV), UV-Vis spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM).

The purpose of this study is to examine the geothermal potential, sustainability, and reinjection possibility of Edremit geothermal field. In order to investigate this, a numerical model consisting of a hot and cold water aquifer system is established. A two dimensional cross sectional model is set to simulate this geothermal system. Different pressure and temperature values are applied to the nodes at the boundaries to perform a steady state calibration which minimizes the computed results and observed values obtained from the near well logs. After the calibration, three alternative scenarios are proposed and the response of the pressure and temperature to these conditions is evaluated. At first the water is pumped from the wells of Yagci, Derman, Entur and ED-3 seperately at a mass rate of 5 kg/s and energy rate of 4.182 x 105 J/s. Then, in scenario 2 the water is pumped at the same rate from all the wells mentioned in the first scenario together. For the third scenario another well is opened to the geothermal system and 80% of the pumped water (temperature being 200C) is injected to the system from the wells while all the wells mentioned are working. The results of these scenarios are utilized to evaluate the reservoir in terms of its response to different production and reinjection conditions. Interpretation of the reservoir response in view of the pressure and temperature declines emphasize that such a simulation study can be applied to assess potential and sustainability of the geothermal systems.

Much experimental evidence shows that the cytoskeleton is a downstream target and effector during cell death in numerous neurodegenerative diseases, including Parkinson's, Huntington's, and Alzheimer's diseases. However, recent evidence indicates that cytoskeletal dysfunction can also trigger neuronal death, by mechanisms as yet poorly understood. We studied a mathematical model of cytoskeleton-induced neuron death in which assembly control of the neuronal cytoskeleton interacts with both cellular stress levels and cytosolic free radical concentrations to trigger neurodegeneration. This trigger mechanism is further modulated by the presence of cell interactions in the form of a diffusible toxic factor released by dying neurons. We found that, consistent with empirical observations, the model produces one-hit exponential and sigmoid patterns of cell dropout. In all cases, cell dropout is exponential-tailed and described accurately by a gamma distribution. The transition between exponential and sigmoidal is gradual, and determined by a synergetic interaction between the magnitude of fluctuations in cytoskeleton assembly control and by the degree of cell coupling. We concluded that a single mechanism involving neuron interactions and fluctuations in cytoskeleton assembly control is compatible with the experimentally observed range of neuronal attrition kinetics. We also studied the transit of neurons through states intermediate between initial viability and cell death. We found that the stochastic flow of neuron fate, from viability to cell death, self-organizes into two distinct temporal phases. There is a rapid relaxation of the initial neuron population to a more disordered phase that is long-lived, or metastable, with respect to the time scales of change in single cells. Strikingly, cellular egress from this metastable phase follows the one-hit kinetic pattern of exponential decline now established as a principal hallmark of cell death in neurodegenerative disorders. Intermediate state metastability may therefore be an important element in the systems biology of one-hit neurodegeneration. Further, we studied the full spatiotemporal dynamics of death factor pulses released from dying neurons to emphasize the effects of the cell-to-cell coupling strength on neuron death rates. The rate of neuron cell loss monotonically increased with increased diffusion-dependent intercellular communication. Death factor diffusion effects may therefore be important moderators of one-hit neurodegeneration.

Heavy load equipment, such as tractors, shovels, cranes, airplanes, etc, often employ fluid power (i.e. hydraulic) systems to control their loads by way of valve adjustment in a pump-valve control configuration. Most of these systems have low energy efficiency as a consequence of pressure losses across throttle valves. Much of the energy is converted into heat energy which can have determinantal effects on component life and the surrounding environment. From an energy efficiency point of view, an ideal hydraulic system is one that does not include any throttling valve. One such circuit is made of a variable pump and motor load (pump/motor configuration). The velocity of the load is controlled by manipulating the pump displacement or by changing the rotary speed of the pump shaft. In such a system, the transient response of the load is often unsatisfactory because it is difficult to quickly and accurately manipulate the pump displacement or change shaft speed. Thus circuit design must be a compromise between the energy efficiency of the pump/motor system and the controllability of a pump/valve/motor combination. One possible compromise is to use a pump-valve configuration which reduces energy losses across the valve. One way to achieve this is by controlling the pressure drop across the valve and limiting it to a small value, independent of load pressure. Based on this idea, a type of hydraulic control system, usually called load-sensing (LS), has recently been used in the flow power area. This type of system, however, is complex and under certain operating conditions exhibits instability problems. Methods for compensating these instabilities are usually based on a trial-and-error approach. Although some research has resulted in the definition of some instability criterion, a comprehensive and verifiable approach is still lacking. This research concentrates on identifying the relationship between system parameters and instability in one particular type of LS system. Due to the high degree of non-linearity in LS systems, the instabilities are dependent on the steady state operating point. The study therefore concentrates first on identifying all of the steady state operating points and then classifying them into three steady state operating regions. A dynamic model for each operating region is developed to predict the presence of instabilities. Each model is then validated experimentally. This procedure, used in the study of the LS system, is also applied to a pressure compensated (PC) valve. A PC valve is one in which the flow rate is independent in variations to load pressure. A system which combines a LS pump and a PC valve (for the controlling orifice) is called a load sensing pressure compensated (LSPC) system. This research, then, examines the dynamic performance of the LSPC system using the operating points and steady state operating regions identified in the first part of the research. The original contributions of this research include: (a) establishment of three steady state operating conditions defined as Condition I, II & III, which are based on the solution of steady state non-linear equations; (b) the provision of an empirical model of the orifice discharge coefficient suitable for laminar and turbulent flow, and the transition region between them; (c) and the development of an analytical expression for orifice flow which makes it possible to accurately model and simulate a hydraulic system with pilot stage valve or pump/motor compensator. These contributions result in a practical and reliable method to determine the stability of a LS or LSPC system at any operating point and to optimize the design of the LS or LSPC system.

Heavy load equipment, such as tractors, shovels, cranes, airplanes, etc, often employ fluid power (i.e. hydraulic) systems to control their loads by way of valve adjustment in a pump-valve control configuration. Most of these systems have low energy efficiency as a consequence of pressure losses across throttle valves. Much of the energy is converted into heat energy which can have determinantal effects on component life and the surrounding environment. From an energy efficiency point of view, an ideal hydraulic system is one that does not include any throttling valve. One such circuit is made of a variable pump and motor load (pump/motor configuration). The velocity of the load is controlled by manipulating the pump displacement or by changing the rotary speed of the pump shaft. In such a system, the transient response of the load is often unsatisfactory because it is difficult to quickly and accurately manipulate the pump displacement or change shaft speed. Thus circuit design must be a compromise between the energy efficiency of the pump/motor system and the controllability of a pump/valve/motor combination. One possible compromise is to use a pump-valve configuration which reduces energy losses across the valve. One way to achieve this is by controlling the pressure drop across the valve and limiting it to a small value, independent of load pressure. Based on this idea, a type of hydraulic control system, usually called load-sensing (LS), has recently been used in the flow power area. This type of system, however, is complex and under certain operating conditions exhibits instability problems. Methods for compensating these instabilities are usually based on a trial-and-error approach. Although some research has resulted in the definition of some instability criterion, a comprehensive and verifiable approach is still lacking. This research concentrates on identifying the relationship between system parameters and instability in one particular type of LS system. Due to the high degree of non-linearity in LS systems, the instabilities are dependent on the steady state operating point. The study therefore concentrates first on identifying all of the steady state operating points and then classifying them into three steady state operating regions. A dynamic model for each operating region is developed to predict the presence of instabilities. Each model is then validated experimentally. This procedure, used in the study of the LS system, is also applied to a pressure compensated (PC) valve. A PC valve is one in which the flow rate is independent in variations to load pressure. A system which combines a LS pump and a PC valve (for the controlling orifice) is called a load sensing pressure compensated (LSPC) system. This research, then, examines the dynamic performance of the LSPC system using the operating points and steady state operating regions identified in the first part of the research. The original contributions of this research include: (a) establishment of three steady state operating conditions defined as Condition I, II & III, which are based on the solution of steady state non-linear equations; (b) the provision of an empirical model of the orifice discharge coefficient suitable for laminar and turbulent flow, and the transition region between them; (c) and the development of an analytical expression for orifice flow which makes it possible to accurately model and simulate a hydraulic system with pilot stage valve or pump/motor compensator. These contributions result in a practical and reliable method to determine the stability of a LS or LSPC system at any operating point and to optimize the design of the LS or LSPC system.

Much experimental evidence shows that the cytoskeleton is a downstream target and effector during cell death in numerous neurodegenerative diseases, including Parkinson's, Huntington's, and Alzheimer's diseases. However, recent evidence indicates that cytoskeletal dysfunction can also trigger neuronal death, by mechanisms as yet poorly understood. We studied a mathematical model of cytoskeleton-induced neuron death in which assembly control of the neuronal cytoskeleton interacts with both cellular stress levels and cytosolic free radical concentrations to trigger neurodegeneration. This trigger mechanism is further modulated by the presence of cell interactions in the form of a diffusible toxic factor released by dying neurons. We found that, consistent with empirical observations, the model produces one-hit exponential and sigmoid patterns of cell dropout. In all cases, cell dropout is exponential-tailed and described accurately by a gamma distribution. The transition between exponential and sigmoidal is gradual, and determined by a synergetic interaction between the magnitude of fluctuations in cytoskeleton assembly control and by the degree of cell coupling. We concluded that a single mechanism involving neuron interactions and fluctuations in cytoskeleton assembly control is compatible with the experimentally observed range of neuronal attrition kinetics. We also studied the transit of neurons through states intermediate between initial viability and cell death. We found that the stochastic flow of neuron fate, from viability to cell death, self-organizes into two distinct temporal phases. There is a rapid relaxation of the initial neuron population to a more disordered phase that is long-lived, or metastable, with respect to the time scales of change in single cells. Strikingly, cellular egress from this metastable phase follows the one-hit kinetic pattern of exponential decline now established as a principal hallmark of cell death in neurodegenerative disorders. Intermediate state metastability may therefore be an important element in the systems biology of one-hit neurodegeneration. Further, we studied the full spatiotemporal dynamics of death factor pulses released from dying neurons to emphasize the effects of the cell-to-cell coupling strength on neuron death rates. The rate of neuron cell loss monotonically increased with increased diffusion-dependent intercellular communication. Death factor diffusion effects may therefore be important moderators of one-hit neurodegeneration.

We propose a situation in which a single employee is responsible for processing incoming claims to an insurance company that can be classified as being one of two possible types. More specifically, we consider a priority-based system having separate buffers to store high priority and low priority incoming claims. We construct a mathematical model and perform queueing analysis to evaluate the performance of this priority-based system, which incorporates the possibility of claims being redistributed, lost, or prematurely processed.

We propose a situation in which a single employee is responsible for processing incoming claims to an insurance company that can be classified as being one of two possible types. More specifically, we consider a priority-based system having separate buffers to store high priority and low priority incoming claims. We construct a mathematical model and perform queueing analysis to evaluate the performance of this priority-based system, which incorporates the possibility of claims being redistributed, lost, or prematurely processed.

The epilepsies are a heterogenous group of symptom complexes, whose common features is the recurrence of seizures. There is no certain therapy for epilepsy. In order to promote new advances for the prevention of epilepsy the molecular mechanism of epileptic activities should be clarified. In the present study the goal is to obtain information for molecular mechanism of epilepsy. To achieve this, molecular alterations from pentylenetetrazol (PTZ)-induced epileptic activities on rat brain tissue and cell membranes were investigated by Fourier Transform Infrared (FTIR) spectroscopy, Fourier Transform Infrared Microscopy and Atomic Force Microscopy (AFM). Moreover, the therapeutic role of an antiepileptic agent vigabatrin (VGB) on epileptic rat brain membranes were examined at molecular level. For better understanding of the action mechanism of PTZ and an antiepileptic drug VGB in cell membranes we firstly studied at model level using multilamellar liposomes (MLVs). We investigated PTZ-DPPC MLVs interactions in terms of lipid phase behavior, order and dynamics and nature of hydrogen bonding around its polar part, using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), Electronspin Resonans Spectroscopy (ESR) and Steady State Fluorescence Spectroscopy. According to our data, PTZ has no ability to interact with membrane lipids. On the other hand, the results of VGB-DPPC interactions showed that VGB strongly interact with the head group and/or the region near the head of membrane phospholipids. The molecular investigation of PTZ-induced epileptic activities revealed that PTZ-induced seizures cause a decrease in the lipid and protein content, membrane fluidity and glycogen level. They stimulate alterations in membrane packing and the secondary structure of proteins as well as lipid peroxidation. In addition, our results show the transcription of early genes following high dose PTZ administration. All these molecular alterations variatins are only resulted from the consequences of epileptic activities not from convulsant agent PTZ itself. The important finding is that, VGB restored some of the alterations by PTZ-induced epileptic activities on brain cell membrane. For instance, it restored membrane fluidity, lipid peroxidation, phospholipid degradation and changes in membrane organization. However, it was found that VGB has no significant effects on the changes in protein secondary structure.