No description available.

A histological investigation was carried out on the esophagus and stomach of the following birds: kingfisher (Megacercyle alcyon caurina), California murre (Uria aalgae Californioa), screeoh owl (Otus asio kennicotti),Peale's faloon (Falco peregrinius pealei), and sparrow hawk (Falco sparverius sparverius). A number of stains was used to intensify the different structures present. In the kingfisher, murre and owl the esophagus was a thin walled highly expansible tube of generally even calibre, whereas in the Peak's falcon the anterior part was dilated into a sac. The wall of the esophagus possessed the usual structure characteristic of this region of the digestive tube. Three types of glands have been distinguished in these birds. In the kingfisher and owl simple oval glands were situated almost entirely within the epithelium. The glands and excretory duct were composed of similar cells with slight variations occuring in excretory duct lengths. In the California murre the glands were still simple but had sunken to the tunica propria with just the excretory ducts passing through the epithelium. The gland cells were narrower, and the duct cells resembled those observed in the owl and the kingfisher. These glands were all apocrine secretory. However, in the Peale’s falcon and sparrow hawk secretion was holocrine. These glands were deeply embedded within the tunica propria and resembled the esophageal glands of the chicken. The excretory ducts were lined by squamous epithial cells. Although the birds examined possessed a variety of food habits: fish, mammals, birds and crustaceans, the food was essentially meat. However, considerable variations in the structure of the esophagus were observed. The proventriculus was comprised of: a mucosa, indented with minute gastric pits, a muscularis externa of three layers of smooth muscle, and a lamina adventitia that surrounded the entire tube. Within the mucosa the deep glands were situated. These were composed of large lobes, enveloped in a dense capsule of connective tissue and internally consisted of simple tubules that radiated about a central excretory duct. The structure of the proventriculus was more or less uniform in the birds investigated, with only slight variations occuring in cellular arrangement and cellular size. The gizzards in the birds examined were large spherical structures that filled a majority of the abdominal cavity. In the kingfisher, owl, Peale's falcon, and sparrow hawk, the gizzard was lined by a thin keratinoid lining that was secreted by the glands present in the gizzard mucosa. The muscularis externa was of approximately equal thickness throughout. In the murre, the keratinoid layer was five or six times as thick as in the above birds and the muscles showed a heavy development on the dorsal and ventral sides. This development took place toward a grinding mechanism that was necessary for processing the hard shelled crustaceans eaten by this bird. / Science, Faculty of / Zoology, Department of / Graduate

京都大学 / 0048 / 新制・課程博士 / 博士(地球環境学) / 甲第21236号 / 地環博第172号 / 新制||地環||35(附属図書館) / 京都大学大学院地球環境学舎地球環境学専攻 / (主査)教授 山下 洋, 教授 柴田 昌三, 教授 宮下 英明 / 学位規則第4条第1項該当 / Doctor of Global Environmental Studies / Kyoto University / DFAM

Rats given L-Ascorbic Acid in their drinking water prior to and during starvation did not develop severe ulceration in the glandular portion of their stomachs. Control rats which were either nontreated or given deactivated L-Ascorbic Acid developed severe stomach pathology on the starvation regimen. The present study was based on the finding that food deprivation results in severe rumenal ulceration in rats. Given that L-AA is essential in maintaining tissue integrity and that ulcers are examples of tissue degeneration, the L-AA in large amounts could retard, or prevent, the formation of starvation induced stomach ulcers in rats. The results show that large amounts of active L-AA were beneficial in maintaining the integrity of rat gastrointestinal tissue exposed to starvation conditions which, in the vitaimin's absence, induces deterioration.

<p> Using maps, correlation and multiple regression, an ecologic analysis was performed to examine the geographic distribution of cancer incidence in ontario with respect to selected ethnic, socio-economic and environmental characteristics for the 10 year period, 1976-1985. Two of the most common causes of cancer deaths, stomach and lung, were studied for each sex separately. The unit of analysis consisted of census divisions. The information used for the cancer were standardized incidence rates from the Ontario cancer Registry. The data for the ecologic variables was obtained from the 1981 Census of Canada. Two of the ecologic variables, education and income (low and median) were used to account for the effects of smoking. </p> <p> Correlation co-efficients were significant for both sites of cancer for males and females for % urban and population density revealing the possibility of a positive relationship with cancer incidence and environmental characteristics. Ethnicity was strongly related to male and female stomach cancer. </p> <p> Significant regression models were obtained for each of the cancer sites using a stepwise procedure with backward elimination. For each of the "best fit" equations, median income and education were included to control for smoking effects. Population density was significant in all equations at the 0.05 level. The percentage urban was significant for all except female stomach cancer. Manufacturing had a negative significant relationship for all cancer sites (male and female). </p> <p> Also included in the study were descriptive statistics and cancer maps to determine the strongest cancer distributions in Ontario. For each site, northern Ontario contained the highest rates. In southern Ontario, urban areas such as Hamilton-Wentworth, and Toronto-York had high rates for all cancers (except Hamilton-Wentworth for male lung cancer). </p> / Thesis / Bachelor of Arts (BA)

No description available.

No description available.

Plasmonic photothermal therapy (PPTT) has a rising promise for treating different cancer cells such as lymphoma or stomach cancer. Technique development of PPTT using metallic nanoparticles is developed upon a modification of the irradiation therapy using two major changes: using a less harmful visible amber light (excluding blue light) and using gold-loaded biocompatible nanoparticles. Acrylate nanoparticles were loaded with desired types of gold nanoparticles at different sizes. The gold-loaded gold nanoparticles were conjugated to cancer cells. By selectively delivering the gold nanoparticles into cancer cells, irradiating a harmless amber visible light will achieve thermal ablation of the cancer cells. Based on imaging spectroscopy, flow cytometry, and cell viability assays, results showed reduction of gold-loaded viable cancer cells upon irradiating with amber visible light, no change in the number of cancer cells with irradiating with light only. On the other hand, DNA intercalation of a trinuclear gold(I), [Au(3-CH3,5-COOH)Pz]3 (Au3) is contrasted with the standard organic intercalators ethidium and ellipticine, as investigated computationally. Frontier molecular orbital energies of intercalators and DNA base pairs were determined and found that all intercalators are good electron acceptors with Au3 being the best electron acceptor having the lowest LUMO. DNA base pairs are better electron donors having the lowest HOMO values, and from the intercalators and base pairs' HOMO/LUMO energies, it is evident the intercalators will overlap with the HOMO of DNA stabilizing the intercalators. Interaction energies (kcal/mol) were obtained as a function of distance, r (angstroms). Results show that the theoretical treatment SDD-WB97XD outperforms SDD-LSDA in both adenine-thymine (AT) systems with ethidium and Au3 intercalators. In both guanine-cytosine (GC) and AT pairs, the Au3 has the lowest interaction energies among these common intercalators, suggesting a potential intercalating drug. Experimental DNA intercalation studies were attempted and methods of finding intercalation binding constants were established, showing gold complexes have better binding constants to DNA than common intercalators to support the computational results.

Orientador: João Pessoa Araujo Junior / Resumo: A suinocultura é uma das principais atividades do agronegócio brasileiro. Por isso, perdas devem ser evitadas a fim de satisfazer a indústria e o bem-estar dos animais. O aparecimento de úlceras gastroesofágicas em suínos causa prejuízos para o produtor e pode levar os animais à morte. O objetivo do trabalho foi verificar a participação de Fusobacterium necrophorum e Helicobacter suis como agentes etiológicos das úlceras nos suínos. Para isso foi realizada avaliação macroscópica e microscópica das lesões, padronização e aplicação de qPCR para diagnóstico bacteriano e visualização de micro-organismos em lâminas de histopatologia coradas por prata, a partir de estômagos de suínos com e sem úlceras. Foram avaliados 126 estômagos, 63 sadios e 63 com úlcera. O DNA extraído de fragmento de tecido da região do pars oesophagea foi utilizado para a análise por qPCR. Foi detectado DNA de F. necrophorum em seis (4,8%) estômagos e H. suis em 16 (12,7%). Os resultados demonstraram que houve diferença quanto a classificação macroscópica e microscópica das lesões. A classificação macroscópica das lesões dos estômagos serve como uma forma de triagem a fim de demonstrar as condições de saúde do animal abatido. A histopatologia pode demonstrar o processo inflamatório desde o seu início, quando a lesão ainda não é visível macroscopicamente favorecendo o rastreamento das possíveis causas das lesões. Os resultados demonstraram associação entre a detecção de H. suis e a presença de úlceras. Para a... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Swine production is one of the main activities of the Brazilian agribusiness and because of that losses must be avoided in order to satisfy the industry and animal welfare. The appearance of gastroesophageal ulcers in swine causes loss to the producer and can lead to animal’s death. The aim of the present study was to verify the participation of Fusobacterium necrophorum and Helicobacter suis as etiological agents of ulcers in swine. Macroscopic and microscopic evaluation of the lesions was performed, qPCR for bacterial diagnose and microorganism visualization in histology slides stained with silver from swine with and without ulcers was also performed. In total, 126 stomachs were evaluated, 63 healthy and 63 with ulcer. The DNA extracted from the tissue fragment of the region pars oesophagea was used for qPCR. DNA of F. necrophorum was detected in six (4.8%) stomachs and H. suis in 16 (12.7%). Results demonstrated that there was statistical difference as to the macroscopic and microscopic lesions classification. Data pointed to an association between H. suis detection and ulcer presence. An association between gravity of the lesions and the presence of bacteria was observed to both microorganisms. The macroscopic classification of the stomachs lesions serves as a form of screening in order to demonstrate the health conditions of the slaughtered animals. Histopathology can demonstrate the inflammatory process from its onset, when the lesion is not yet visible macroscopically ... (Complete abstract click electronic access below) / Doutor

Chu Wai Kit. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2006. / Includes bibliographical references (leaves 87-96). / Abstracts in English and Chinese. / Acknowledgments --- p.ii / Publication --- p.iii / List of Abbreviations --- p.iv / List of Tables --- p.v / List of Figures --- p.vi / Abstract --- p.vii / 摘要 --- p.x / Table of Contents --- p.xii / Chapter Chapter 1 --- Introduction --- p.1 / Chapter 1.1 --- Epidemiology of gastric cancer --- p.1 / Chapter 1.2 --- Risk factors associated with gastric cancer --- p.7 / Chapter 1.3 --- Prevention of Gastric Cancer --- p.9 / Chapter 1.4 --- H. pylori eradication and gastric cancer development --- p.11 / Chapter 1.5 --- Non-steroidal anti-inflammatory drugs and gastric cancer prevention --- p.13 / Chapter 1.6 --- COX-2 independent pathway --- p.14 / Chapter 1.7 --- Animal model of gastric cancer --- p.15 / Chapter 1.8 --- Microarray system --- p.16 / Chapter 1.9 --- Hypothesis --- p.18 / Chapter 1.10 --- Aim of study --- p.19 / Chapter Chapter 2 --- Chemoprevention of gastric cancer by celecoxib --- p.20 / Chapter 2.1 --- Introduction --- p.20 / Chapter 2.2 --- Material and Methods --- p.22 / Chapter 2.2.1 --- Animals --- p.22 / Chapter 2.2.2 --- Chemicals --- p.22 / Chapter 2.2.3 --- Study design --- p.23 / Chapter 2.2.4 --- Cell Culture --- p.24 / Chapter 2.2.5 --- Celecoxib treatment --- p.24 / Chapter 2.2.6 --- Cell proliferation assay --- p.25 / Chapter 2.3 --- Results --- p.26 / Chapter 2.3.1 --- Chemoprevention of gastric cancer by celecoxib in rats --- p.26 / Chapter 2.3.2 --- Effects of celecoxib on growth of human gastric cancer cells --- p.29 / Chapter 2.4 --- Discussion --- p.30 / Chapter 2.4.1 --- MNNG induced gastric cancer effectively --- p.30 / Chapter 2.4.2 --- Celecoxib significantly suppressed gastric carcinogenesis in rats --- p.31 / Chapter 2.4.3 --- Celecoxib inhibited the growth of MKN 45 in a concentration-dependent manner --- p.31 / Chapter 2.4.4 --- Celecoxib may exert its anti-tumor property through COX independent pathway --- p.32 / Chapter Chapter 3 --- Gene expression profiles of celecoxib treated rat gastric tumor and human gastric cells --- p.34 / Chapter 3.1 --- Introduction --- p.34 / Chapter 3.2 --- Material and Methods --- p.34 / Chapter 3.2.1 --- RNA extraction --- p.34 / Chapter 3.2.2 --- Target preparation and Array hybridization --- p.35 / Chapter 3.2.3 --- Post-hybridization processing and Scanning --- p.36 / Chapter 3.2.4 --- Microarray data analysis --- p.36 / Chapter 3.2.5 --- Quantitative RT-PCR --- p.37 / Chapter 3.3 --- Results --- p.39 / Chapter 3.3.1 --- Gene expression profiles of rat gastric tumors --- p.39 / Chapter 3.3.1.1 --- Genes differentially expressed in MNNG induced gastric tumors --- p.39 / Chapter 3.3.1.2 --- Genes differentially expressed in celecoxib treated group --- p.42 / Chapter 3.3.1.3 --- Mechanisms underlying chemoprevention of celecoxib --- p.43 / Chapter 3.3.2 --- Verification of gene expression by quantitative RT-PCR --- p.55 / Chapter 3.3.3 --- Confirmation of the gene expression profiles in human by quantitative RT-PCR --- p.59 / Chapter 3.4 --- Discussions --- p.63 / Chapter Chapter 4 --- Effects of celecoxib on Akt pathway in gastric cancer cells --- p.68 / Chapter 4.1 --- Introduction --- p.68 / Chapter 4.2 --- Material and methods --- p.72 / Chapter 4.2.1 --- Protein extraction --- p.72 / Chapter 4.2.2 --- Western blotting --- p.72 / Chapter 4.3 --- Results --- p.74 / Chapter 4.3.1 --- Expression of the Akt pathway after treatment with celecoxib --- p.74 / Chapter 4.4 --- Discussions --- p.78 / Chapter Chapter 5 --- Conclusion --- p.82 / References --- p.87