The aim of the research documented in this thesis was to explore issues associated with the development of instrumentation for life detection and characterisation in a planetary exploration context. Within this aim, the following objectives had to be achieved: 1. To consider current and near-future single molecule detection (ultra-low lower limit of detection) analytical techniques that would be compatible with development into a Space qualifiable in situ analytical instrument for the detection of biomarkers in a planetary exploration context. 2. To practically consider the consequences of Planetary Protection and Contamination Control on the development of a sample return instrumentation in a planetary exploration context. 3. To consider the implications of flying an in situ instrument on-board a stratospheric balloon platform in order to apply them into a specific planetary exploration mission: In order to achieve the objectives described above, the following work was pursued:  A desk-based European Space Agency (ESA) study was carried out which entailed producing a literature review on single molecule detection technologies that had to be validated by the expert community. This was done by organising an International Workshop on Single Molecule Detection Technologies for Space Applications in March 2009 at Cranfield University, UK. The approved technologies then had to be analysed with standard analytical techniques (i.e., tradeoffs) in order to propose a specific technology for development and present its breadboard implementation and test plans at the end of the study.  A sample return experiment implementing PP&CC constraints and protocols was designed, built, tested and flown on-board the ESA, Swedish Space Corporation (SSC), Swedish National Space Board (SNSB) and German Space Agency (DLR) BEXUS stratospheric balloon platform. The biological and engineering results obtained from the sample return flight were then analysed and lessons learnt obtained for future flights.  Another desk-based study was performed to research future stratospheric balloon platforms for the exploration of Venus’ cloud layer. The in situ instrument previously proposed for the detection of biomarkers for planetary exploration missions was then put forward as a possible payload for a Venusian stratospheric balloon platform and approved by experts during the Venus Exploration Analysis Group (VEXAG) conference held in August 2011 in Washington D.C, USA. The first part of the research involved studying ultra-low lower limit of detection technologies as these have the potential to impact significantly on the technological and scientific requirements of future Space missions. Two systems were proposed: one based on Tandem Mass Spectrometry (with Cylindrical Ion Trap analysers) followed by Surface Enhanced Raman Scattering spectroscopy to create an MS/MS-SERS instrument for the detection of astrobiology biomarkers in Martian regolith, Europan ice and samples from Titan’s hydrocarbon lakes; and a second one as a Stand-Alone SERS system for the detection of biomarkers in Enceladean plumes, Venusian clouds and cometary coma. The second part of the research practically explored the design of instrumentation for stratospheric balloon platforms. CASS•E, the Cranfield Astrobiological Stratospheric Sampling Experiment, was a life detection experiment that aimed to be capable of detecting stratospheric microorganisms. The experiment consisted of a pump which drew air from the Stratosphere through a 0.2 μm collection filter which retained any microorganisms and >0.2 μm particulates present in the pumped air. Due to the expected rarity of microbes in the Stratosphere compared to the known levels of contamination at ground level, Planetary Protection and Contamination Control (PP&CC)constraints were introduced. Therefore PP&CC protocols were followed to implement Space qualified cleaning and sterilisation techniques; biobarrier technology was implemented to prevent re-contamination of the instrument after sterilisation; and cleanliness and contamination was monitored throughout assembly, integration and testing. The third part of the research demonstrated how an instrument from the first part of the study could be proposed as a payload on-board a stratospheric balloon platform with a focused mission context, i.e., a life detection mission for Venus. Therefore, the research concluded with the proposal of a payload for a Venus mission based on SERS technology on-board a stratospheric balloon platform to search for life above or in the mid Venusian cloud cover.

Scientific stratospheric balloons offer a valuable service to scientists wishing to test or demonstrate developing technological instruments, or to run fully operational instruments with short preparation times and for a cheap price compared to other similar services. The ballooning industry is therefore a vital part of the scientific community as it enables less funded and experienced scientific organisations to actively engage in the development of their technologies. In the context of scientific stratospheric balloons, the speed of permeation affects among other things the flight time and the flight planning, as these are determined by the rate of loss of the buoyancy force keeping the balloon afloat. Most balloons today use helium as lifting gas, but the ballooning industry is today facing increased pressure to switch to hydrogen gas. Before making this switch, understanding how hydrogen gas behaves differently, in terms of permeation or otherwise, is important to prevent unexpected flight paths among other things. In this thesis, two experiments were conducted in order to attempt to determine the ratio of permeation speed between hydrogen gas and helium through balloon membranes. One experiment used a manometric method, where the pressure of permeant in a diffusion chamber was measured over time. The other experiment measured the buoyancy force of permeant-filled balloons over time. The resulting ratio of permeation speed may give more confidence in predicting how much faster or slower a stratospheric balloon filled with hydrogen will lose buoyancy force.

This thesis describes the development of an autonomous recovery system for stratospheric balloons, focusing on the novel pressurised balloon prototype BALMAN of CNES. Stratospheric balloons, reaching altitudes of up to 40 km, are utilised for scientific experiments, with recovery of payloads being a critical aspect. While traditional recovery methods involve separating the balloon envelope and deploying a parachute, BALMAN's parachute will be deployed in free fall. The proposed autonomous system comprises decision-making and electronics components. The decision-making segment employs microcontrollers and environmental sensors to recognise the balloon's descent, triggering the release decision. The electronics section, responsible for providing energy to a pyro cutter, is designed with electrical switches and capacitors. Thermal simulations guide the placement of heaters, maintaining system temperature within operational limits. The final prototype, tested for functionality on-ground, exhibits a measured energy release of 24 mJ, double the requirement for pyro cutter activation. However, environmental and flight testing remain pending. The system's potential applications extend beyond BALMAN, offering a standardised autonomous recovery solution for various balloons. This innovation promises enhanced landing accuracy, obviates the need for telecommunication in recovery, and facilitates payload descent deceleration. Future endeavors involve comprehensive testing and potential integration into BALMAN missions, showcasing the system's adaptability and operational simplicity across diverse stratospheric endeavors.

JEM-EUSO est un projet de télescope spatiale dédié à la détection des rayons cosmiques d'ultra-haute énergie (RCUHE) (d'énergie supérieure à 10/48 eV) par l'observation de l'émission de lumière ultra-violette produite par l'interaction entre li rayon cosmique et l'atmosphère terrestre. Dans le cadre de ma thèse, j'ai travaillé sur le premier démonstrateur du projet, EUSO-Balloon, une version réduite de l'instrument prévu pour JEM-EUSO portée par un ballon stratosphérique. J'ai participé à l'étalonnage de la surface focale du ballon, composée de 36 photo-multiplicateurs multi-anodes (MAPMT), ainsi qu'à l'intégration de l'électronique de lecture et l'assemblage et les tests de l'instrument complet. J'ai pris part à la campagne de vol qui s'est déroulée à Timmins, au Canada, pour un vol la nuit du 24 août 2014. Pendant le vol, l'instrument a pu observer le flux lumineux en ultra-violet émis et réfléchi par le sol, ainsi que des impulsions laser tirées à partir d'un hélicoptère volant sous l'instrument pendant une partie de la mission et simulant le signal émis par un RCUHE interagissant avec l'atmosphère terrestre. Après le succès du premier vol d'EUSO-Balloon, un second vol est prévu au printemps 2017. Ce vol est prévu pour durer plusieurs semaines, et a pour objectif principal l'observation de RCUHE. Pour préparer ce vol, et à la suite des retours de la première mission, j'ai participé à plusieurs campagnes de tests afin d'améliorer certains aspects technologiques de l'instrument. J'ai également mené des simulations afin d'estimer le nombre d'UHECR que l'instrument détectera pendant un vol de plusieurs semaines / JEM-EUSO is a future space UV telescope dedicated to the observation of Ultra-High Energy Cosmic Rays( UHECR), through 'the detection of the UV light emitted by the interaction between the UHECR and the Earth atmosphere. The work done during my PhD was focused on EUSO-Balloon, a smaller scale balloon borne prototype of JEM-EUSO with a complete detection chain and Fresnel optics. During my PhD, I took part in the calibration of the focal surface, made up of 36 mufti-anode photomultipliers as well as the integration and full scale tests of the read-out electronics and the whole instrument. I took part of the flight campaign in Timmins, Canada with a flight on the 24`11 of August 2014. During the flight, the instrument was able to observe the UV light emitted and reflected by the ground as well as laser pulses shot from an helicopter flying under the balloon during the first part of the flight to simulate UHECR signal as seen from the instrument. After the success of the first flight of EUSO-Balloon, a second flight o a several weeks is planned for spring 2017, with the goal of observing real UHECR events from above. I took part of several test campaigns to improve the performances of the instrument for the second flight. Finally, I mn a serie of simulations to estimate the number of events the instrument should be able to detect during a several-week flight