Oxygen uptake rates by fine benthic organic matter were measured on samples from two Appalachian mountain streams to determine the effects of land use patterns on microbial respiration. Both Coweeta Creek in western North Carolina, and Wolf Creek in western Virginia, flow through national forest, agricultural land and scattered residential areas. Seven sites were sampled at six to ten week intervals over one year. Land use was determined using infrared aerial photographs. ARC/INFO was used to obtain land use areas for various land uses. Oxygen uptake rates were measured using a Gilson differential respirometer, at ambient temperature and 20°C. Temperature was the most important factor influencing oxygen uptake rates in both streams, with oxygen uptake rates consistently lower at ambient temperatures than 20°C. Oxygen uptake rates were higher in Wolf Creek than Coweeta Creek and were higher at sites that drained agricultural areas. Nitrates increased as the percent of agriculture increased in Wolf Creek, but not in Coweeta Creek. Nitrates were positively correlated with oxygen uptake / Master of Science

This study examined the consequences of the opening and operation of a new highway north of Richmond, Virginia with respect to contamination of the aquatic environment with heavy metals (Zn, Cd, and Pb), and the effects of these metals on the biota of roadside streams. Sixteen sites located on six small, soft-water streams that were crossed by the highway, encompassing six reference sites located upstream of the highway, six sites located directly at the highway, and four sites located downstream of the highway, were sampled over a two and a half year period, allowing both spatial and temporal analyses. Traffic densities on the highway averaged about 12,000 vehicles per day (vpd). Significant increases in the metals concentrations of sediment, benthic invertebrates, fish whole-bodies, and fish tissues (liver, kidney, and bone) were noted over the course of the study, although the increase varied in magnitude, and were not always consistent. Sediment metals concentrations followed a dynamic plateau. Fish whole-body concentrations of Cd and Pb increased steadily over the course of the study. Spot-sampling for the same parameters along another nearby, more heavily traveled highway (50,000 vpd) indicated that increases in metals concentrations in the different ecosystem components at the study streams would have been greater had there been more traffic. A number of biotic parameters were investigated to determine whether metals contamination was affecting the biological integrity of the study sites. These were: benthic macroinvertebrate diversity and density; the percentage of the aquatic insect community that was composed of chironomids; and fish community diversity, density, and biomass. Only benthos density, the percent chironomids, and fish species diversity showed changes that could be related to metals contamination. Indications from spot sampling along the more heavily traveled highway were that if more contamination had been experienced, more biotic parameters would have been disturbed, and to a larger extent. Fish community structure analyses using the Pinkham-Pearson coefficient of similarity indicated that fish community structure became increasingly altered at highway sites, and to a lesser degree downstream sites, over the study period. / Ph. D. / incomplete_metadata

Thesis (PhDEng)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: The interaction between moving water and the physical attributes of a river, as displayed by the channel morphology, determines the availability of physical habitat for aquatic species and thus also the condition of the ecosystem. As such, the environmental flow assessment process requires knowledge on how changes in the flow regime will affect both the morphological and hydraulic conditions within a river channel. With the increasing development of water-related infrastructure in mountain regions, knowledge of the morphological and hydraulic characteristics of rivers in the upper catchment areas is very important. Cobble and boulder bed rivers in the Western Cape are typical examples. They are characterized by steep gradients, great variability in sediment size and relatively low flow depths. The bed configuration contains a series of pools, steps, rapids, riffles and plane beds, while energy losses are high as a result of turbulence and hydraulic jumps. Due to their wideranging morphological and associated hydraulic attributes, the physical habitats within these rivers are extremely diverse, both on a spatial and temporal scale. This study addresses the interaction between moving water and the physical attributes of cobble and boulder bed rivers. Empirical, semi-empirical and theoretically based models are developed which define the hydraulic and morphological related characteristics of environmental flow components in cobble and boulder bed rivers. They cover macro scale channel deformation, the scouring of sand from the interstitial spaces between the cobbles as well as velocity-depth relationships, which prove to be key components in the assessment of environmental flow requirements in cobble and boulder bed rivers. / AFRIKAANSE OPSOMMING: Die wisselwerking tussen die vloeiende water en die fisiese eienskappe van 'n rivier, soos beskryf deur die morfologie, bepaal die beskikbaarheid van habitat vir akwatiese spesies en gevolglik ook die welstand van die ekosisteem. Kennis omtrent die impak van 'n veranderde vloei regime op beide die morfologiese en die hidrouliese toestande in 'n rivierloop is dus nodig vir die bepaling van omgewingsvloeibehoeftes. Met die toenemende ontwikkeling van water-verwante infrastruktuur in bergagtige gebiede is 'n grondige kennis van die morfologiese en hidrouliese eienskappe van riviere in hierdie bo-opvanggebiede gebiededend noodsaaklik. Spoelklip riviere in die Wes-Kaap is tipiese voorbeelde van sulke riviere. Hierdie riviere word gekenmerk deur steil hellings, 'n wye verskeidenheid sedimentgroottes, relatiewe lae vloeidieptes en hoë energieverliese as gevolg van turbulensie. Verder bevat die ri vierbed afwisselend poele en stroomversnellings en gevolglik word 'n wye verskeidenheid habitat tipes in hierdie riviere aangetref. Hierdie studie fokus op die interaksie tussen bewegende water en die fisiese eienskappe van spoelklip riviere. Met behulp van empiriese, semi-empiriese en teoretiese modelle word die morfologiese en hidrouliese eienskappe van omgewingsvloeibehoeftes in spoelklip riviere aangespreek. Dit sluit makroskaal kanaal vervorming, die uitskuur van sand tusen die spoelklippe asook die verwantskap tussen vloeisnelheid en vloeidiepte in. Hierdie aspekte kan beskou word as van die sleutel elemente vir die bepaling van omgewingsvloeibehoeftes in spoelklip riviere.

In 2000 the European Union introduced the Water Framework Directive, new legislation that regulates the use of surface waters within the European Community. The goal of this legislation is to protect, enhance and restore all surface waters within the Community to Good Surface Water Status. Good-Status is described as having low levels of anthropogenic distortion in its hydro-morphological and physiochemical components as well as possessing biota that would normally be associated with the type-specific aquatic ecosystem. The assessment of ecosystem status is to be defined by comparisons with intact representative reference sites, by using modelling techniques that define reference conditions, a combination of the two, or expert judgement. As undisturbed aquatic ecosystems are rare or non-existent in Europe the base-line data will have to be defined using the latter methodologies. The aim of this project is to help define reference conditions for lotic systems in Europe based on the physical instream habitat parameters of a resident species. Brown trout (Salmo trutta), a ubiquitous and well studies species endemic to Europe, was used as the target organism to develop the assessment protocol. The project focused on the requirements this species has of aspects of its physical habitat; specifically, its usage of depth, velocity, and substrate. An extensive survey of the scientific literature was used to define the requirements trout has for the three physical parameters at four life stages. These are the spawning, nursery, juvenile and adult-resident life stages. These requirements were expressed as tolerance profiles, which defined suitable, usable and not-suitable habitat. The methodology was demonstrated by evaluating the physical habitat available at six reaches in three small streams, March, Burnhouse and Bin Burns, which drain into the Carron Valley Reservoir in central Scotland. From the perspective of water depth, these streams seem best suited as nursery areas, are less well suited as juvenile habitat, and do not appear to be well matched for adult residents. The assessment of both velocity and substrate indicated that the portion of the study reaches available for use by resident brown trout increased with trout size. The assessment of all three physical habitat parameters at all study reaches found variable portions of the streams suitable for use by spawning trout. When the habitat variables are integrated all stream segments streams seem best suited as nursery and spawning areas. To a lesser extent juvenile trout can use these burns and very little habitat is available for use by adult resident trout. The tolerance profiles that were created in this study are standardized assessment criteria that when compared with stream survey data can produce an appraisal of habitat availability in any fluvial freshwater system that supports populations of brown trout (Salmo trutta). The assessment method can be combined to produce an integrated habitat assessment, using both an index and by the calculation of Froude number, which is a more realistic approach than the assessment of individual habitat parameters as salmonids choose their microhabitat based on multiple factors. This approach allows an investigator to determine the amount and relative portion of useable habitat and to determine the quality of that habitat. Finally, by examining the physical habitat variable that most strongly correlates with the final integrated habitat distribution the individual habitat parameter that is most important to the distribution of physical habitat at a site can be determined. While this technique would certainly benefit from further development it does show potential to aid in physical habitat assessment of trout streams.

M.Sc. (Zoology) / The Limpopo Province covers an area of 12.46 million hectares and these accounts for 10.2 per cent of the total land area of the Republic of South Africa. The province is endowed with abundant agricultural resources and it is one of the country’s prime agricultural regions noted for the production of fruits and vegetables, cereals, tea, and sugar. A key feature of the agricultural industry of Limpopo Province is its dualism. There are two distinct types of agricultural production systems. The large scale commercial farming system occupies approximately 70% of the total land area. The smallholder farms are located mostly in the former homeland areas and they cover approximately 30% of the provincial land surface area. The town of Thohoyandou, with its surrounding villages, is the area of greatest human concentration in the Luvuvhu Catchment and subsistence farming is about a third of the total agricultural component. It is important to study the effects of agricultural inputs (e.g. fertilizers and sediment loads) on aquatic ecosystems in order to fully understand the processes involved of these stresses on aquatic ecosystems. Knowledge of these impacts toward the environment and human health is often limited due to lack of capacity building, especially among small scale farmers. Ten bio-monitoring sites were studied on five systems in the Vhembe district. The sites were sampled during the low flow period of November 2011 and the high flow period of April 2012. Sampling sites were selected to present conditions in the Mutale, Mutshindudi and Tshinane Rivers upstream and downstream of the potential influence of small scale agricultural activities...

Resumo: Os peixes apresentam uma grande diversidade de espécies, sendo na região Neotropical onde se encontra o maior número de espécies de água doce. Entretanto, esta grande diversidade encontra-se ameaçada devido à degradação dos ecossistemas lóticos, principalmente pela alteração do habitat devido à destruição das florestas e fragmentação da rede hidrográfica. As florestas, principalmente as florestas ripárias, possuem funções ecológicas importantes para a estruturação dos riachos e das comunidades aquáticas. Por muito tempo, as florestas têm sido extensamente destruídas, sendo substituídas principalmente pelos usos agrícolas, ocasionando a destruição dos ecossistemas e da biodiversidade aquática. Além das alterações nas florestas, a presença de represamentos e cruzamentos de estradas gera impactos adicionais para os peixes ao romper a conectividade existente na rede hidrográfica. Os peixes possuem ciclos de vida complexos que dependem da complexidade estrutural do riacho e da conectividade entre trechos montante e jusante da rede. Portanto, a conservação das florestas e a manutenção da conectividade hidrológica são fatores importantes para a conservação das comunidades de peixes. Neste trabalho, avaliou-se a diversidade das comunidades de peixes de riachos em função das alterações na estrutura da paisagem florestal (composição e configuração) e da conectividade da rede hidrográfica em paisagens agrícolas. O estudo foi realizado em microbacias de 1ª a 4ª ordem pertencentes à bacia do rio Corumbataí. Diferenças nos índices de diversidade das comunidades foram testadas a partir da Análise de Variância considerando a estrutura da paisagem florestal em diferentes escalas espaciais (microbacia, rede de drenagem e trecho de coleta) e a conectividade da rede hidrográfica. As relações entre as variáveis da paisagem (estrutura da paisagem... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Fish exhibit great species diversity, and the Neotropical region holds the largest number of freshwater fish species . However, this great diversity is threatened due to lotic ecosystems degradation, mainly through forest destruction and stream network fragmentation. Forests, mainly riparian forests, have important ecological functions for stream and aquatic communities structuring. For a long time, forests have been extensively destroyed and replaced mainly by agricultural uses, causing stream and aquatic biodiversity destruction. Besides the alterations in forests, the presence of dams and road crossings generates additional impacts to fish by disrupting existing connectivity to the stream network. Fish have complex life cycles that depend of the stream structural complexity and the connectivity between upstream and downstream sections of the stream network. Hence, forest conservation and maintenance of hydrological connectivity are important factors for the conservation of fish communities. In this study, we evaluated stream fish community diversity in relation to changes in forest landscape structure (composition and configuration) and the stream network connectivity in agricultural landscapes. The study was conducted in 1st and 4th order catchments belonging to the Corumbatai river basin. Differences in diversity indices were tested by Analyses of Variance considering forest landscape structure at different spatial scales (whole catchment, stream network and stream sampled reach) and stream network connectivity. The relationship between landscape variables (forest landscape structure, riparian forest landscape structure and physical and hydrological hierarchy variables) and aquatic environment variables (channel structure and limnology and fish diversity indices) were evaluated using Redundancy Analysis (RDA) and Multiple Regression Analysis. These relationships were also assessed... (Complete abstract click electronic access below) / Orientador: Silvio Frosini de Barros Ferraz / Coorientador: Pedro Gerhard / Banca: Lílian Casatti / Banca: Maurício Cetra / Mestre

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Following the implementation of the Water Framework Directive (WFD) integrated catchment management plans must be prepared for all river basins, in order to achieve 'good ecological status' (GES) in all EU waters. This concept is a broader measure of water quality than the chemical and biological measures, which were previously dominant in EU water policy. The Directive also calls for a consideration of the economic costs and benefits of improvements to ecological status in catchment management plans, along with the introduction of full social cost pricing for water use. In this thesis, the primary focus is on the use of the Choice Experiment (CE) method. The CE method is reviewed and then used to estimate the value of improvements in a number of components of ecological status on two Irish waterways (the Boyne and the Suir). Apart from CE method another stated preference approach to environmental valuation is also considered; the Contingent Valuation Method (CVM). This thesis determines what value the targeted population of the two catchments place on the nonmarket economic benefits of moves towards GES by employing both approaches and various model specifications, while the applicability of Benefit Transfer (BT) method is also assessed under different tests. In addition, the design of the questionnaire used in the survey stage of the research, offered the possibility of investigating issues related to the effect of cognitive ability and psychometric factors on choice. Respondents with discontinuous preferences are identified and analysis is conducted to investigate the implications of not accounting for these preferences. Finally, due to experiencing protesting behaviour by a proportion of the sampling population an attempt is made to investigate the parameters that contributed to this inclination.

This thesis examines the ecological consequences of sewage disposal in aquatic ecosystems. Sewage simultaneously represents a form of enrichment and a source of stress to a receiving system. Enrichment effects dominate when sewage loading does not exceed the capacity of the system to assimilate waste, as is presently the case in the St. Lawrence River. We developed a method to quantify the assimilation of sewage-derived organic matter by riverine biota, then used this method to examine the pathways by which sewage enhances secondary production in the St. Lawrence. We showed that the relative importance of dissolved nutrients and particulate organic matter to the river food web is to a large degree dictated by the physical and biological characteristics of the local environment. The effects of this enrichment on the receiving water community were also influenced by habitat characteristics. Analysis of body size distributions revealed that only the largest organisms had higher densities at enriched sites, and that the identity of these organisms depended on habitat structure. Densities of smaller organisms were controlled by a combination of habitat characteristics and feeding interactions. This pattern was consistent with food web models of top-down (consumer) control. At each trophic level, relatively invulnerable (large) prey achieved higher densities, whereas densities of more vulnerable (small) prey were controlled by their predators. Finally, we synthesized the ideas outlined above to predict how conditions in the St. Lawrence will change in the future as a result of lower water levels. More extensive macrophyte cover, slower current, and warmer temperatures in a shallower St. Lawrence will all enhance the ability of the system to physically retain and biologically process sewage nutrients, but will also increase the likelihood of negative effects such as anoxia.