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Research in the multidisciplinary science of ecotoxicology is crucial to assess injuries to ecosystem resources from chemical spills or other stressors used to support environmental decision-making. Established guidelines recommend the use of non-standard native species in toxicity investigations. This work focused on the use of native species for aquatic toxicity assessment to make more relevant conclusions on the potential for adverse biological effects to occur as a result to single chemical exposures or exposures to a complex mixture like oil. We apply these studies to investigate petroleum product impacts from the Deepwater Horizon incident and concerns for metal toxicity in estuarine environments using a new model organism. Data generated from comprehensive toxicity testing programs were used in the first probabilistic risk assessment of Deepwater Horizon oil toxicity highlighting a lack of appropriate data and representative phyla. Novel toxicity study methods and a stress-response index were developed and demonstrated sensitivity and success in using the starlet anemone in ecotoxicology studies. Swim performance was used as new method to investigate sublethal indicators of stress resulting in varied responses from sheepshead minnows and Florida pompano. These studies further our ability for better laboratory-to-field extrapolation and for decision-making. The use of native species and complex mixtures like oil presented novel challenges in conducting aquatic toxicity studies. Special emphasis is placed on the necessity to understand the appropriate laboratory conditions for native species not typically held in the laboratory and maintaining study parameters to obtain quality data for more accurate interpretation and replication.

Objectives: To determine the physiological strain on industrial workers under thermal stress on extended shifts. To continuously measure deep body core temperatures, heart rates, fluid intake, changes in hydration state and physical fatigue in order to establish acceptable levels of physiological strain. To develop a rational heat stress index compatible with these limits. To design working-in-heat protocols for a self-paced workforce. Methods: A series of studies was conducted over 77 shifts on a group of approximately 50 male volunteers working in thermally stressful environments. Continuously- recorded deep body core temperatures, heart rates, fluid consumption, urinary specific gravity and physical fatigue were measured and recorded. A new field protocol was developed to assess physical fatigue over the working shift. An original methodology was developed to allow any heat stress index to be assessed on a comparative basis with any other index. A review of the commonly used occupational heat stress indices was conducted. A new rational heat stress index was developed, based on existing biophysical relationships and recommended physiological strain limits of deep body core temperature and sweat rate. New protocols designed for self-paced work incorporating the significant risk factors for heat illness were developed and implemented in a workforce of approximately 2000 workers exposed to heat stress. The previous protocols used a shortened shift as the primary intervention to protect worker health. The subsequent protocols removed the shortened shift and replaced this with a range of other interventions. Deep body core temperature, heart rate, fluid consumption, hydration state and fatigue were measured before and after the changes in protocols. / Results: Comparisons of heat stress indices confirmed the wide divergence in guidance provided by many of the commonly-used indices in terms of acceptable working environments. It also highlighted a number of serious shortcomings in the most widely-used indices, especially WBGT and ISO7933. A new, rational heat stress index called Thermal Work Limit (TWL) was developed. This included development of a computer model incorporating key thermal physiological parameters (deep body core temperature, mean skin temperature, sweat rate, skin wettedness). There was no increase in heat stress (as indicated by average workplace environmental conditions), deep body core temperature, mean heart rate, or changes in hydration status after the changes in protocols. Average environmental conditions were severe (WBGT 30.9° C, sd 2.0° C, range 25.7-35.2° C). Environmental conditions in the study were much hotter than those considered acceptable under standards such as the ACGIH. The results showed that miners regularly exceeded those limits allowable under most current indices in terms of maximum deep body core temperature (avg 38.3° C, std dev 0.4° C), maximum temperature rise (1.4° C, 0.4° C) and maximum heat storage (431 kJ, 163 kJ), without reporting any symptoms of heat illness. A significant component of the observed elevated core temperatures was due to the normal circadian rhythm, which was measured at 0.9° C (std dev 0.2° C). Evidence was found that workers "self-pace" when under thermal stress. Fluid intake averaged 0.8 l/h during exposure (sd 0.3 l/h, range 0.3-1.5 1/h). Average urinary specific gravity at start-, mid- and end of shift was 1.0251, 1.0248 and 1.0254 respectively; the differences between start and mid-shift, mid and end-shift, and start and end-shift were not significant. / However, a majority of workers were coming to work in a moderately hypohydrated state (urinary specific gravity avg 1.024, std dev 0.0059). Involuntary dehydration was not found to occur in the study group. This is in contrast to several other studies and some of the leading heat stress standards, which are based on the premise that workers are unable to maintain their hydration status when working in the heat, even when their fluid consumption is equal to their sweat rate. Continuous heart rates measured over a shift (avg 103 bpm, 14% of shifts exceeding avg 110 bpm, 5% exceeding avg 120 bpm) were in excess of those allowable under most current indices On average, workers experienced a peak 10- minute heart rate of 140 bpm and a peak 30-minute heart rate of 130 bpm during their shifts. There was a significant increase in fatigue in the first half of the working shift (P=0.001), with workers on average showing a significant recovery in the second half of their shift (p=0.04). Conclusions: Current heat stress indices provide little common agreement as to acceptable levels of thermal strain or stress for workers, at equivalent levels of environmental stress. IS07933 is seriously flawed and the ACGIH WBGT guidelines are too conservative for acclimatised workers and are unlikely to become widely adopted by industries with well-acclimatised workers. Many of the existing indices show internal inconsistencies. / Most of the physiological heat strain limits used in existing rational heat stress indices (in terms of deep body core temperature and heart rate) are conservative for self-paced, acclimatise d, non-dehydrating male workers. Involuntary dehydration is not unavoidable when acclimatised workers are exposed to thermal stress. Heat stress standards should not limit heat exposure durations for self- paced workers who have access to water on the basis of an unavoidable body water loss. Physical fatigue does occur in workers under heat stress on extended shifts; however, most workers show a significant increase in fatigue in the first half of their shift; whereas data indicates self-paced workers undergo significant recovery in terms of fatigue in the second half of the shift. As the heat exposures in this study cover a wide range of temperatures, humidity levels, wind speeds, body morphology and VO2max, these conclusions are applicable to most thermally stressful settings involving well-informed, well-acclimatised and self-paced male workers. The major category of work type not covered by this study is that of workers in fully-encapsulated (vapour-barrier) protective clothing. In addition, this study examined acute effects of heat stress and strain, not effects that might only be manifest with chronic exposure to heat.

Dans de nombreux pays, l’aquaculture de pectinidés dépend aujourd’hui du succès de la production contrôlée de juvéniles. Néanmoins, les fortes variations des taux d’éclosion des oeufs et de la survie larvaire, enregistrées à ce jour, rendent cette production imprévisible. Les élevages larvaires en flux ouvert de coquilles Saint-Jacques (Pecten maximus) ont été développés en Norvège et présentent des résultats prometteurs. Malheureusement, les rendements de production encore faibles et l’impossibilité de travailler à fortes densités restent un frein majeur au développement de cette technique. En France, une technique en flux-ouvert, en petit volume (5 L), et à forte densité (≤ 300 larves mL-1) a été développée pour les ostréidés. Des expériences préliminaires visant à décliner ce système d’élevage aux larves de P. maximus se sont avérées infructueuses : retard de croissance et forte mortalité en quelques jours. Il est reconnu que les larves de pectinidés doivent faire face à des contraintes diverses en écloserie : bactériologiques, physiologiques et environnementales. Elles sont également plus sensibles que les larves des autres espèces de bivalves comme par exemple l’huître japonaise (Crassostrea gigas). Il apparait donc nécessaire aujourd’hui d’identifier plus clairement l’origine des phénomènes perturbant le bon développement des larves en flux ouvert afin d’améliorer la qualité des élevages et les rendements larvaires. Grâce à l’étude et à la compréhension des mécanismes physiologiques impliqués dans la lutte contre le stress des larves de P. maximus en flux ouvert, ce projet de doctorat donne des clés permettant d’améliorer cette technique d’élevage tout en limitant l’utilisation de produits chimiques en milieu contrôlé. / In many countries, aquaculture of pectinids depends on the success of artificial spat production in hatchery. This production is always unpredictable due to the variability of hatching rate and larval survival. Flow-through larval rearing systems were developed in Norway for the King scallop Pecten maximus and showed promising results. Unfortunately the system needs to be optimized since the larval yields and the densities used are still relatively low. In France, a small-scale (5 L) and high-density (≤ 300 larva mL-1) flow-through larval rearing system was successfully developed for oysters. First trials in such system and in similar conditions with P.maximus failed as we registered slower growth and high mortality rate in only a few days. It is known that pectinids larvae are more sensitive to environmental conditions than the oyster Crassostrea gigas, for example.Nowadays, it is important to identify and to understand the phenomena disturbing larval development in flowthrough system to improve larval quality and production yields. This doctoral project provided some indications how improving P. maximus flowthrough rearing system while limiting the use of antibiotic through a better understanding the physiological mechanisms involved in the larval response to a stressful environment

Diploma thesis deals with the issue of systemic risk and its impact on the financial system. In terms of the explanation of the individual regularities analyses principles of systemic risk and its impact on the financial sector. The first part of this work is dedicated to a complex analysis of the systemic risk sources and a description of different measurement methods among others also dedicated to detection of systemically important institutions. The analytical part demonstrates an application of one of the model for systemic risk measurement on the real data from the United States of America between years 1990 and 2011 and the analysis of the newly adopted Dodd-Frank Act regulation. The main merit of this work is to describe and evaluate the complex perspective of the systemic risk, which is a prerequisite for its successful application and management.

L’Europe a subi la crise la plus sévère de sa récente histoire à la suite de la crise financière globale de 2008. C’est pourquoi cette thèse a l’objectif d’identifier de façon empirique les déterminants de cette crise dans le cadre de 15 principaux membres de l’UE. Dans ce sens, nous développons d’abord un index de pression fiscale continu, contrairement aux travaux empiriques précédents, afin d’identifier des périodes de crise dans les pays UE-15 de 2003 à 2015. Ensuite, nous utilisons trois différentes techniques d’estimation, à savoir Cartes auto-organisatrices, Logit et Markov. Nos résultats d’estimation démontrent que notre indicateur de crise identifie le timing et la durée de la crise de dette dans chacun des pays de UE-15. Résultats empiriques indiquent également que l’occurrence de la crise de dette dans l’UE-15 est la conséquence de la détérioration de balances macroéconomiques et financières sachant que les variables comme le ratio des prêts non-performants sur les crédits totaux du secteur bancaire, la croissance du PIB, chômage, balance primaire / PIB, le solde ajusté du cycle PIB. De plus, variables démontrant la qualité de gouvernance tel que participation et responsabilisation, qualité de la réglementation, et de l'efficacité gouvernementale, jouent également un rôle important dans l’occurrence et sur la durée de la crise de dette dans le cadre de l’UE-15. Étant donne que les résultats économétriques indiquent l’importance de la détérioration fiscale dans l’occurrence de la crise de dette européenne, nous testons la convergence fiscale des pays membre de l’UE. Les résultats montrent que Portugal, Irlande, Italie, Grèce et Espagne diverge des autres pays de l’UE-15 en termes de dette publique / PIB alors qu’ils convergent, à part la Grèce, avec les autres pays membres de l’UE-15 en termes de déficit budgétaires / PIB. / Europe went through the most severe economic crisis of its recent history following the global financial crisis of 2008. Hence, this thesis aims to empirically identify the determinants of this crisis within the framework of 15 core EU member countries (EU-15). To do so, the study develops a continuous fiscal stress index, contrary to previous empirical studies that tend to use event-based crisis indicators, which identifies the debt crises in the EU-15 and the study employs three different estimation techniques, namely Self-Organizing Map, Multivariate Logit and Panel Markov Regime Switching models. Our estimation results show first that the study identifies correctly the time and the length of the debt crisis in each EU-15-member country by developing a fiscal stress index. Empirical results also indicate, via three different models, that the debt crisis in the EU-15 is the consequence of deterioration of both financial and macroeconomic variables such as nonperforming loans over total loans, GDP growth, unemployment rates, primary balance over GDP, and cyclically adjusted balance over GDP. Besides, variables measuring governance quality, such as voice and accountability, regulatory quality, and government effectiveness, also play a significant role in the emergence and the duration of the debt crisis in the EU-15. As the econometric results clearly indicate the importance of fiscal deterioration on the occurrence of the European debt crisis, this study also aims to test the fiscal convergence among the EU member countries. The results indicate that Portugal, Ireland, Italy, Greece, and Spain diverge from other EU-15 countries in terms of public debt-to-GDP ratio. In addition, results also show that all PIIGS countries except for Greece converge to EU-10 in terms of budget deficit-to-GDP ratio.

This dissertation is composed of four essays that empirically investigate three topics in financial economics; financial stress and its leading indicators, the relationship between bank competition and financial stability, and the link between management board composition and bank risk. In the first essay we examine which variables have predictive power for financial stress in 25 OECD countries, using a recently constructed financial stress index. We find that panel models can hardly explain FSI dynamics. Although better results are achieved in country models, our findings suggest that financial stress is hard to predict out-of- sample despite the reasonably good in-sample performance of the models. The second essay develops an early warning framework for assessing systemic risks and predicting systemic events over two horizons of different length on a panel of 14 countries. We build a financial stress index to identify the starting dates of systemic financial crises and select crisis-leading indicators in a two-step approach; we find relevant prediction horizons for each indicator and employ Bayesian model averaging to identify the most useful predictors. We find superior performance of the long-horizon model for the Czech Republic. The theoretical literature gives conflicting predictions on how bank...