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Selection of new eggplant (Solanum melongena, L.) linesArias, Iliana Corría 15 June 2011 (has links)
In den letzten Jahren ist das Interesse der Europäer am Verbrauch „Exotischer Gemüsearten“ gewachsen wie z.B. für asiatische Auberginengenotypen, die sich in der Farbe, im Geschmack und der Form von den standardmäßigen dunklen violetten Auberginenfrüchten unterscheiden. Diese Entwicklung kann die Einführung und die Kommerzialisierung neuer asiatischer Auberginensorten in Westeuropa beeinflussen und dadurch die Vielfalt des Angebots mit diesem Gemüse in den gemäßigten Regionen erhöhen. Aus diesem Grund bestand das Ziel dieser Arbeit darin, einige asiatische Genotypen (hauptsächlich vietnamesischen Ursprung) hinsichtlich ihres Wachstums und Ertrags zu untersuchen und diese mit typischen „europäischen“ Sorten bei einer Kultivierung im Gewächshaus zu vergleichen. Außerdem sollte geprüft werden, ob die genetische Variabilität von Samenherkünften verwendet werden könnte, um Klone zu selektieren, die den Wachstumsbedingungen in den Gewächshäusern unter den Klimabedingungen in Deutschland angepasst sind. In den in vitro Experimenten ist die Reaktion von 5 Auberginengenotypen auf die Mikrovermehrung und auf die in vitro Verfahren ausgewertet worden. Zusätzlich wurden die Einflüsse von Wachstumsregulatoren, NAA und 2.4D, auf die Kallus- und Organbildung und indirekte Pflanzenregeneration in vitro untersucht, um Aussagen hinsichtlich der Nutzbarkeit von biotechnologischen Züchtungsmethoden, zu erhalten. Das in vitro Klonen wurde mit dem Ziel durchgeführt, Klone von ausgewählten 5 asiatischen Auberginengenotypen zu erhalten, um Aussagen zu ihrer vegetativen und generativen Entwicklung sowie dem Ertrag zu erhalten, und mit typischen „europäischen“ Sorten unter Gewächshausbedingungen zu vergleichen. Alle Genotypen wurden einer der hydroponischen Substratkultur im Gewächshaus kultiviert. Die Entwicklung der Genotypen in organischen und mineralischen Substrat wurde verglichen. Das organische Substrat hat zum besseren Wachstum aller Auberginengenotypen geführt. Die aus der in vitro Vermehrung erhaltenen Klone der asiatischen Auberginengenotypen entwickelten sich zu normalen Pflanzen, die einen typischen Habitus erreichten und teilweise einen Ertrag hatten, der mit dem der Kontrolle vergleichbar war. Die besten asiatischen Auberginenklone hatten ähnliche Ergebnisse wie die der Kontrolle sowohl im Stadium des Blühbeginns, als auch im Ertrag. Ein wichtiges Merkmal für die Auswahl einiger asiatischer Klone war ihre Fähigkeit frühzeitig zu blühen, vergleichbar mit den „europäischen“ Sorten. Die asiatischen Genotypen waren charakterisiert durch schmalere und leichtere Früchte mit einem guten Geschmack, weniger Samen und einer guten Konsistenz des Fruchtfleisches im Vergleich mit den „europäischen“ Sorten. Ausgehend von den Ergebnissen die in den Untersuchungen erzielt wurden, scheint es möglich, neue stabile Genotypen zu erhalten, die geeignet sind für eine Kultivierung in der „Substratkultur“ im Gewächshäusern und die ein Potenzial für den Erwerbsanbau unter europäischen Bedingungen haben. Für eine Optimierung der Wachstumsbedingungen, insbesondere das Formierungssystem, sind weitere Forschungsarbeiten erforderlich zumal diese Genotypen sehr starkwüchsig sind. Des Weiteren sollte der optimale Erntezeitpunkt und die Fruchtqualität untersucht werden. / Recently, there has been a growing interest of the Europeans in the consumption of „exotic vegetables“ like those of Asian eggplant genotypes different in colour, taste and shape from the traditional dark violet eggplant fruits. This may influence the introduction and commercialisation of Asian eggplant types in Western Europe, which will contribute to increase the biodiversity of this crop in temperate regions. Therefore, this work aimed at screening 4 Asian eggplant genotypes (mainly of Vietnamese origin) concerning their growth and yield in greenhouses in comparison to „European“ breeds. Moreover, it should be tested whether the genetic variability of seed progenies could be used to select plants adapted to the growing conditions in greenhouses under the climatic peculiarities in Germany. In in-vitro experiments the response of 5 selected eggplant genotypes to micropropagation and in-vitro manipulation has been evaluated. Further on the influence of plant growth regulators, e.g. NAA and 2,4 D, on the callus and organ formation, and indirect plant regeneration in-vitro were studied in preparation of use of biotechnological breeding methods later on. The in-vitro cloning was carried out with the aim to produce clones of 5 Asian eggplant genotypes to evaluate their vegetative and generative development, as well as the yield, in comparison with typical „European“ varieties under greenhouse conditions. All genotypes were cultivated in „substrate culture“ with drip irrigation. The influence of organic and mineral substrates on the growth and development of the eggplant genotypes was compared. The organic substrate favoured better growth of all eggplant genotypes. The in-vitro derived clones of Asian eggplant genotypes developed to normal plants that reached full maturity and some of them had a yield comparable with that of the controls, the typical „European“ varieties. The best Asian eggplant clones gave similar results as the controls in the beginning of flowering and the yield. The early flowering feature characteristic of some clones of Asian origin comparable to that of the typical „European“ varieties is very important for selection. Asian genotypes were characterized by smaller and lighter fruits having good taste, less seeds and good consistency in comparison with the „European“ varieties used as control. Derived from the results obtained in this research, it seems possible to obtain new stable genotypes for „substrate culture“ system in greenhouse with a potential for commercial production under European conditions. The optimization of growing conditions especially the pruning system needs further research for these very vigorously growing plant types. Further on studies regarding optimal harvesting time and fruit quality shall be done.
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Use of bio-waste as fertiliser for the protected vegetable cultivationBöhme, Michael Henry 22 February 2019 (has links)
The number of biogas plants in Germany is increasing from 3,711 in 2007 to 8,075 in 2016. In these biogas plants, it occurred more than 50 Mt digestate. Therefore, several investigations are started to use digestate as organic fertiliser mostly for field crop cultivation. Experiment with tomatoes was carried out were digestate was used as a supplement to the growing media in an amount of 5%, 15%, and 25%, compared with a treatment of mineral fertiliser and lupine wholemeal. The tomato yield was highest in the treatment with mineral fertilisation, the yield with 25% digestate was only a little lower. More experiments are necessary for particular regarding the amount and frequency of fertilization with digestate from biogas plants. In Germany and in Vietnam the number of sheep flocks is increasing, high amounts of uncleaned sheep wool are available. Because of the high amount of nutrients - especially nitrogen -, sheep wool pellets could be used as multi-functional fertiliser in vegetable cultivations. Four types of sheep wool pellets have been tested in protected cultivation. Tomatoes were cultivated in a greenhouse using substrate culture with perlite, bark compost, sheep wool slabs, respectively, and sheep wool pellets as fertiliser. Best growth and highest yield for tomatoes were obtained using pine bark and perlite as a substrate, both fertilised with sheep wool pellets. Based on the results of the yield and the analyses of the nutrient content in plants it seems that sheep wool pellets can be used, for the cultivation of vegetables in greenhouses. / Số lượng các nhà máy biogas tại CHLB Đức tăng từ 3.711 năm 2017 lên 8.075 năm 2016. Các nhà máy biogas sản sinh ra hơn 50 triệu tấn chất thải. Vì vậy đã có nhiều nghiên cứu liên quan đến sử dụng nguồn chất thải này làm phân bón hữu cơ cho canh tác nông nghiệp. Thí nghiệm với cà chua sử dụng chất thải biogas làm chất bổ sung dinh dưỡng cho giá thể trồng cây theo các tỷ lệ 5%, 15% và 25% đối chứng với công thức sử dụng phân hóa học và bột nguyên vỏ họ đậu. Năng suất cà chua thu được từ các công thức bổ sung chất thải biogas đều cao hơn đối chứng, chỉ có công thức bổ sung 25% có năng suất thấp hơn. Tuy nhiên vẫn cần có những nghiên cứu tiêp theo về lượng và tần xuất sử dụng bón phân với chất thải từ nhà máy biogas. Ở Đức và ở Việt Nam số lượng đàn cừu đang tăng lên, một lượng lớn lông cừu phế phẩm phát sinh. Với hàm lượng dinh dưỡng cao, đặc biệt là nitơ, viên nén từ lông cừu phế phẩm có thể sử dụng làm phân bón đa chức năng cho trồng trọt. Nghiên cứu đã sử dụng 4 loại viên nén lông cừu làm phân bón trong điều kiện trồng có kiểm soát. Cà chua được trồng trong nhà kính với 3 loại giá thể là perlite, vỏ cây thông đã ủ hoai, thảm lông cừu với phân bón là viên nén từ lông cừu phế phẩm. Năng suất cao nhất và đem lại sinh trưởng tốt nhất cho cây cà chua là công thức sử dụng vỏ cây thông và perlite. Dựa trên kết quả về năng suất và phân tích dinh dưỡng trong cây và sản phẩm, nghiên cứu cho thấy sự phù hợp của viên nén từ lông cừu phế phẩm làm phân bón cho canh tác rau trong nhà kính.
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