Feeds often contain molybdenum (Mo) and sulfur (S) in excess of recommended allowances for adequate copper (Cu) absorption by ruminant livestock. Two randomized-block experiments were conducted using lambs given a cereal-based diet (90% of dry matter (DM) intake), containing 8 mg Cu, 0.7 mg Mo and 2.1g S per kg DM, that was either unsupplemented or supplemented with ammonium molybdate or sodium sulfate alone or in combination, to determine the effects of Mo, S and Mo+S on growth, hematology, serum Cu and Mo concentrations and reproductive function. Short term intake (4 weeks) by rams, aged 18 and 20 weeks, of 26 mg Mo alone or in combination with 2g S per kg DM, had no effect on growth, hematology or the concentration of total Cu in serum (TCu). However, supplemented groups had lower (P<0.05) concentrations of serum Cu soluble in trichloroacetic acid (TCA-Cu), but the group given Mo+S had the highest (P<0.05) concentration of serum residual Cu (RCu). Luteinizing hormone (LH) peak amplitude was affected by age*diet interaction (P<0.05), because of high amplitude LH peaks in the serum of lambs, aged 24 wks, given Mo alone. Testosterone serum secretory profiles did not differ among diet groups, but testosterone peak frequency was higher (P<0.05) for older ram lambs. Long term intake (32-39 wks) by ewe, ram and wether lambs (gonadal influence) of 12 mg Mo, or 2g S alone or combined per kg DM also had no effect on hematology or TCu. Food intake and liver weights were higher (P<0.05), but TCA-Cu was lower (P<0.05) for groups given S. Serum concentrations of total Mo (TMo) were higher (P<0.05) for Mo-supplemented groups, but RCu was highest only for the Mo+S group. Growth was affected by Mo*S*gonadal influence*time interaction (P<0.05); until autumn, the body weight of Mo-supplemented groups were higher than those of ram and wether lambs given Mo+S. Ovarian or testicular functions were delayed more severely for Mo- than Mo+S-supplemented groups. Mean concentrations of LH were affected by Mo*S*gonadal influence*time interaction (P<0.05); the mean LH values of Mo-supplemented ram and ewe lambs were higher than the Mo+S-supplemented group, whereas a reverse trend was observed for wether lambs. The amplitude of LH peaks was affected by Mo*S*gonadal influence*time interaction (P<0.05); for ram lambs, LH peak amplitude was higher for Mo than Mo+S-supplemented groups, whereas for wether and ewe lambs the reverse trend was noted. Mean concentrations of serum Cortisol and Cortisol peak amplitude were affected by Mo*S interaction (P<0.05); the mean Cortisol concentration and peak amplitude for the Mo-supplemented group were higher than those for the Mo+S-supplemented group. The frequency of LH and Cortisol peaks did not differ (P>0.10) among diet groups. In conclusion, Mo supplementation of cereal-based diets containing a high concentration of Cu did not adversely affect lamb growth. However, the effect of high dietary Mo on reproductive function appears to depend on the dietary level of S and the induction of high serum RCu (thiomolybdate). Further investigations on the effects of Mo and thiomolybdate on endocrine function may provide a nutritional basis for improving reproductive efficiency in ruminants. / Land and Food Systems, Faculty of / Graduate

The effects of three fertilization rates of N (0, 112 and 224 kg/ha) as ammonium nitrate and S (0, 34, and 67 kg/ha) as calcium sulphate on yield, chemical composition, digestibility and palatability of orchardgrass (Dactylis glomerata L.) , were studied in a field experiment. Treatments were applied in a randomized block design with a 3x3 factorial arrangement of treatments with four replications. Hay was harvested on 18 Aug. 1982 and fed to sheep in metabolism trials. One half of the initial amount of fertilizer was reapplied and forage was stockpiled. Stockpiled growth was ensiled on 6 Nov. 1982 and was fed to sheep in palatability trials. Fertilizer was reapplied at the initial rates on 27 Apr. 1983 and yield and mineral concentrations were determined on 6 June 1983. Dry matter yield increased in response to N (P<0.01). Nitrogen fertilization decreased NDF (P<0.05) ADF, cellulose (P<0.01) and total S (P<0.01) and increased nitrate-N (P<0.01) in hay and stockpiled growth. In stockpiled growth S increased ADF ( P<0.05) and cellulose quadratically ( P<0.001) at the 34 kg/ha rate. Sulphur increased ( P<0.05) No₃-N in hay but decreased (P<0.05) it in stockpiled growth. The N:S ratio was increased (P<0.01) by N fertilization and decreased (P<0.05) by S fertilization. Nitrogen and S had a quadratic effect (P<0.0001) on lactic acid and water-soluble carbohydrates in silage. Digestibility or palatability of silage was not influenced by N or S. / Master of Science

The effects of red clover (Trifolium pratense, L.), three levels of N (0, 224 and 448 kg•ha⁻1•yr⁻1, as a split application) and two levels of S (0 and 34 kg•ha⁻1•yr ) on the chemical composition and dry matter digestibility of an orchardgrass (Dactylis glomerata, L.) sward were studied. The treatments were replicated three times in a randomized block design with a split-plot arrangement of treatments. Crude protein content, in vitro dry matter digestibility and dry matter yield were increased (P<. 01) with increasing levels of N. Increasing levels of N decreased (P<.05) S content of orchardgrass. The inclusion of red clover did not have any significant effect on the components analyzed, probably due to a low proportion of red clover (approximately 8%) in the mixed sward. Application of S increased ( P<.05) the S content of orchardgrass and decreased N-to-S ratio at all levels of N application. Application of S increased (P<.05) the crude protein and in vitro dry matter digestibility, whereas neutral detergent fiber, lignin and hemicellulose were decreased (P<. 05). Nitrogen fertilization had a quadratic effect (P<.05) on in vivo digestibility of dry matter, acid detergent fiber and hemicellulose and a linear effect (P<.001) on apparent digestibility of crude protein. Inclusion of red clover increased (P<.01) in vivo digestibility of dry matter and hemicellulose, and decreased (P<.01) digestibility of acid detergent fiber and lignin. Application of S had no direct effect on in vivo digestibility of orchardgrass. A linear interaction (P<.01) between N and S was observed for dry matter and crude protein digestibility. Rumen fluid ammonia-N was increased (P<.05) and butyric acid concentration was decreased (P<.05) linearly by N fertilization. / Master of Science

Sulfur (S) deficiencies have become an increasing problem in the United States. A field experiment, in a latin square design, was conducted to investigate effects of S fertilization as 0 and 67 kg/ha as a single or split application on corn (Zea mays L.) forage yield and chemical composition. Sulfur fertilization by either method increased yield of whole plant and grain 7% and increased number of plants with two ears. Total S and sulfate-S concentration in whole corn plants, leaf, stem, and grain were increased with S fertilization. Corn forages were ensiled at harddent stage (35% dry matter). Sulfur fertilized corn silages (N/ S=42 and 43) and non-S fertilized silage (N/ S=62) supplemented at two rates with sodium sulfate (N/ S=l2 and 45) were fed to sheep in metabolism and palatability trials. Both experiments were conducted as a randomized block design with six replications per treatment. All silages were supplemented with urea (6.7 g/ d). Digestibility of dry matter and cell wall components and apparent absorption of Sand N were increased with S fertilization and S supplementation. Nitrogen retention was increased 14% by S supplementation (N/S=l2) and 31% by S fertilization. Sheep fed N/ S=12 silage had lower blood hematocrit and hemoglobin levels then those fed S fertilized or N/ S=45 silages. Blood urea-N levels were higher in sheep fed S fertilized silages. Increasing dietary S by fertilization or supplementation had no measurable effect on dry matter intake. / M.S.