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Role of Serum Albumin Aggregation in Lubrication and Wear Protection of Shearing SurfacesSamak, Mihir 11 July 2019 (has links)
Healthy articular joints exhibit remarkable lubrication due in large part to the complex rheological and tribological behavior of the synovial fluid (SF) that lubricates the joints. Current approaches that seek to elucidate such remarkable lubrication usually focus on the roles of high molecular weight SF components such as lubricin and hyaluronic acid but frequently overlook the role of serum albumin (SA), although it represents 90% of the protein content of SF. In this thesis, we used the Surface Forces Apparatus to investigate in detail the structural and tribological response of SA thin films when sheared between model surfaces and subjected to a large range of shearing parameters. Our data indicate that, under shear, SA films reproduce closely the shear response previously reported for SF, i.e., film thickening and formation of numerous long-lived aggregates accompanied by low friction and efficient surface protection against damage. More specifically, our detailed investigation of shear parameters reveals that (i) strong anchoring of SA to surfaces promotes the formation of large rod-like shaped aggregates that enable rolling friction and keep surfaces far apart, preventing damage, (ii) aggregation mechanism is irreversible, which makes aggregates long-lived (though mobile) in the contact, and (iii) aggregate formation only occur when SA was sheared above a ‘critical’ amplitude Ac and a critical shear velocity Vc.
Collectively, our results provide experimental evidence of the role of globular proteins, such as SA, in lubrication and establish a correlation between shearing parameters, formation and stability of aggregates, low friction and wear protection. Although our findings are based on experiments involving rigid, nonporous surfaces hence can hardly be generalized to compliant and porous cartilage surfaces, they are applicable to other rigid tribosystems such as artificial joints and will certainly advance our understanding of joint implants’ lubrication in SF mediated by protein aggregation, with implications for future design of artificial joints and therapeutic interventions.
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Probing Molecular Interactions of Comb-type Polymers in Air/Water/Solids InterfacesZhang, Ling Unknown Date
No description available.
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Μελέτη της αλληλεπίδρασης μεταξύ προσροφημένων πολυμερικών στρωμάτωνΧιωτέλης, Ιωάννης 17 July 2014 (has links)
Στην παρούσα διδακτορική διατριβή έγινε μελέτη πολυμερικών βουρτσών που διαμορφώνονται από συμπολυμερή πολυστυρενίου-πολυβουταδιενίου που φέρουν στην άκρη τους ομάδες ικανές να προσροφηθούν (PS-PB-Zw) Η μελέτη έγινε κυρίως με την τεχνικής της μέτρησης δυνάμεων (SFA) και της ανάκλασης νετρονίων. Ιδιαίτερο ενδιαφέρον παρουσιάζουν οι γραμμικές πολυμερικές αλυσίδες όπου ένα μεγάλου μοριακού βάρους πολυμερές φέρει στην άκρη του μια μόνο ακραία ομάδα. Τα γραμμικά αυτά συμπολυμερή είχαν μελετηθεί και στο παρελθόν. Το ζητούμενο όμως ήταν να μεταβάλλουμε την αρχιτεκτονική αυτή και να παρατηρήσουμε τις πιθανές μεταβολές στα ποιοτικά χαρακτηριστικά των προσροφημένων πολυμερικών στρωμάτων που διαμορφώνουν.
Ως μέτρο σύγκρισης μελετήσαμε αρχικά τις ήδη μελετημένες γραμμικές αλυσίδες και στη συνέχεια προχωρήσαμε στη μελέτη των νέων δειγμάτων. Προσθέσαμε δύο και τρεις ακραίες ομάδες στο άκρο της πολυμερικής αλυσίδας μεγάλου μοριακού βάρους και αναμέναμε σύμφωνα με θεωρητικές προβλέψεις αύξηση στο προσροφημένο ποσό. Η αύξηση αυτή θα ήταν εμφανής μέσω της αύξησης του πάχους του πολυμερικού στρώματος που εύκολα μπορεί να μετρηθεί με την τεχνική της μέτρησης των δυνάμεων αλληλεπίδρασης. Επίσης μετρήσαμε άμεσα το προσροφημένο ποσό και με την τεχνική της ανάκλασης νετρονίων για πολυμερή τριών διαφορετικών μοριακών βαρών (25000, 70000 και 150000). Το σημαντικότερο αποτέλεσμά μας είναι ότι δεν παρατηρήσαμε αύξηση στο προσροφημένο ποσό σε σχέση με τις γραμμικές αλυσίδες και σε χρόνους ενός τυπικού πειράματος. Υπήρχε ωστόσο η υποψία ότι σε βάθος χρόνου μπορεί να αυξάνεται το προσροφημένο ποσό. Για το λόγο αυτό διεξαγάγαμε μια σειρά από μετρήσεις «κινητικής» με την τεχνική της ανάκλασης νετρονίων, αλλά και με συμπληρωματικές τεχνικές (διέγερση επιφανειακών πλασμονίων). Καμία μεταβολή δεν παρατηρήσαμε στο προσροφημένο ποσό, ούτε σε βάθος χρόνου αλλά ούτε και με την προσθήκη επιπλέον ακραίων ομάδων ικανών να προσροφώνται σε επιφάνειες.
Στη συνέχεια επεκτείναμε τη μελέτη μας στην «αντίστροφη» αρχιτεκτονική. Διατηρήσαμε μια ακραία ομάδα και προσθέσαμε δύο και τρεις πολυμερικές αλυσίδες μεγάλου μοριακού βάρους (Μr=70000). Η αρχιτεκτονική που διαμορφώσαμε με τον τρόπο αυτό ήταν ουσιαστικά αρχιτεκτονική αστεροειδών πολυμερών με μικρό αριθμό πλοκαμιών. Στη περίπτωση αυτή παρατηρήσαμε μια σταδιακή μείωση του προσροφημένου ποσού με την προσθήκη επιπλέον βραχιόνων στο αστέρι. Η μείωση αύτη έχει παρατηρηθεί τόσο από μετρήσεις δυνάμεων, όσο και από μετρήσεις με ανάκλαση νετρονίων. Στα προφίλ δυνάμεων παρατηρούμε διαφοροποιήσεις σε σχέση με την γενική εικόνα που εμφανίζουν οι πολυμερικές βούρτσες. Αυτές οι διαφοροποιήσεις μπορούν να ερμηνευτούν με τη βοήθεια θεωρητικών προβλέψεων για τη συμπεριφορά των αστεροειδών πολυμερών. Η μείωση του προσροφημένου ποσού με αύξηση του αριθμού των πλοκαμιών μπορεί να αποδοθεί είτε σε λόγους κινητικής, είτε σε λόγους αλληλεπίδρασης μεταξύ των πολυμερικών αλυσίδων και μεταξύ των πλοκαμιών ενός μακρομορίου.
Επίσης στη παρούσα διδακτορική διατριβή μελετήσαμε τη συμπεριφορά προσροφημένων ασθενών πολυ-ηλεκτρολυτών. Οι πολυ-ηλεκτρολύτες συγκεντρώνουν σταδιακά την προσοχή όλο και περισσότερων ερευνητών καθώς εμφανίζουν αρκετές σημαντικές εφαρμογές και είναι συμβατοί με βιολογικά συστήματα. Μέχρι πριν λίγο καιρό οι ασθενείς πολυ-ηλεκτρολύτες, όπως είναι το πολυακρυλικό οξύ που μελετούμε στη παρούσα εργασία, δεν είχαν μελετηθεί εκτενώς. Η μελέτη τους παρουσιάζει δυσκολίες, λόγω της πολυπλοκότητας στη συμπεριφορά τους. Αυτή εξαρτάται τόσο από τη συγκέντρωση άλατος του διαλύματος, όσο και από το pH. Μεταβάλλαμε τόσο τη συγκέντρωση άλατος του διαλύματος, όσο και το pH του διαλύματος εξετάζοντας πως αυτά επηρεάζουν το πάχος του προσροφημένου στρώματος και τα ποιοτικά χαρακτηριστικά των πολυ-ηλεκτρολυτικών προσροφημένων στρωμάτων. Παρατηρήσαμε λοιπόν μείωση του πάχους της πολυ-ηλεκτρολυτικής βούρτσας με αύξηση της συγκέντρωσης άλατος. Το αποτέλεσμα αυτό είναι πλήρως συμβατό με θεωρητικές προβλέψεις.
Στη συνέχεια εξετάσαμε τη συμπεριφορά των ασθενών πολυ-ηλεκτρολυτών μεταβάλλοντας το pH του διαλύματος. Παρατηρήσαμε ότι με αύξηση του pH οι πολυμερικές αλυσίδες εκτείνονταν αυξάνοντας το πάχος της πολυμερικής βούρτσας. Αυτή η συμπεριφορά είναι άμεσο αποτέλεσμα της αύξησης του βαθμού διάστασης των πολυμερικών αλυσίδων. Αυξάνοντας το pH περισσότερα φορτία αποσπώνται από την πολυμερική αλυσίδα φορτίζοντάς την έτσι ισχυρότερα. Οι απωστικές ηλεκτροστατικές αλληλεπιδράσεις μεταξύ των φορτίων εξαναγκάζουν την αλυσίδα σε έκταση επιμηκύνοντας την πολυμερική βούρτσα. Τα αποτελέσματα αυτά είναι επίσης σε πλήρη συμφωνία με θεωρητικές προβλέψεις. / Flexible polymer chains end-tethered to a surface in good solvent tend to extend away from the surface due to excluded volume interactions. At sufficiently high grafting densities the chains become elongated normal to the surface, this extension being opposed by an elastic restoring force of entropic origin to form a layer of stretched chains referred to as a “polymer brush”. These systems have been studied extensively in recent years by numerous experimental techniques and theoretical methods. In the present investigation we have studied asymmetric star-shaped polymers whereby the different arms are either non-adsorbing PS chains or short PB chains terminating in a zwitterionic end-group known to adsorb strongly on surfaces such as mica or quartz. In this manner, it is possible on the one hand to form brushes with a single PS chain, but multiple zwitterionic stickers, and on the other to study the reverse case of multiple non-adsorbing arms attached to a surface via a single zwitterions. We have used the surface force balance technique to determine the interaction between such brush-layers formed on mica and neutron scattering to determine the absorbed amount and interanchor distance.
Interactions between polymer brushes formed by highly asymmetric star-like polymers with a long PS arm and one, two or three short PB arms each terminating in a zwitterionic end-group were studied in order to explore the effect of the sticking energy on the brush structure. Polystyrene stars with two and three tails bearing a single end group were also studied to investigate how the height of the adsorbed layer and the grafting density are affected. Our measurements show no significant differences between PS with 1, 2 and 3 end groups. This may be due to kinetic reasons since additional polymer chains are hindered from attaching to a brush-bearing surface. The behavior of two PS chains with one end-group ((PS)2-PB-X) seems not to differ appreciably from that of PS-PB-X copolymer. On the other hand three PS chains with one end-group ((PS)3-PB-X) appeared to form layers with smaller brush height and greater interanchoring distance, relative to PS-PB-X layers. We can attribute this to the extra stretching that the three-chain architecture imposes on the adsorbed brush.
In the present investigation we have also studied weak polyelectrolytes. Polyelectrolytes (charged polymer chains) remain among the least understood materials despite their importance in biology (proteins, DNA) and materials science. Their behavior and characteristics are not yet fully understood because of complicated correlations due to their charged nature that gives rise to long-range interactions. The counterplay of their properties as polymers and electrolytes with counterions around polyelectrolyte chains imposes additional difficulties on explaining their behavior. The association of counterions around polyelectrolytes and the pH of the solution are two parameters than can affect the properties of such systems. Especially weak polyelectrolytes (like poly-acrylic acid) are even sensitive to pH changes because of the alterable degree of dissociation. We measured forces between two charged polymer layers of Poly (isoprene-acrylic acid) diblock copolymers adsorbed on mica surfaces. Poly (isoprene-acrylic acid) diblock copolymers can be adsorbed from one end (poly-isoprene) which is sorter than the poly-(acrylic acid) part. The properties of the polymer layer at various salt concentrations and different pH of the solutions were measured by Surface Forces Apparatus. Information about the height of the polymer layer, and the adsorbed amount were extrapolated. The extracted results were compared with theoretical predictions showing well fit.
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Greffage chimique de molécules et de polymères sur des substrats de mica et étude de leurs propriétés de surfaceLiberelle, Benoît January 2007 (has links)
Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal.
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Greffage chimique de molécules et de polymères sur des substrats de mica et étude de leurs propriétés de surfaceLiberelle, Benoît January 2007 (has links)
Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal
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A Study of the Structure and Dynamics of Smectic 8CB Under Mesoscale ConfinementBenson, James January 2012 (has links)
The structure and dynamics of the smectic-A liquid crystal 8CB (4 cyano-4 octylbiphenyl) when sheared and confined to mesoscale gaps (with crossed cylindrical geometry and mica confining surfaces) were studied using a Surface Forces Apparatus (SFA). Triangular shear patterns with frequencies of 0.01, 0.1, 1.0 and 10 Hz, and amplitudes of 62.5 nm, 625 nm and 6.25 m were applied to samples at gap sizes of 0.5 and 5.0 m. The study was performed at room temperature (20.5C) and at two higher temperatures (22C and 27C). In order to minimize the thermal fluctuations within the test chamber and hence to allow for the rapid re-initialization of test runs, the SFA was modified to allow for quick, precise and remote control of the confining surfaces. The procedure maximized the number of tests that could be undertaken with a single pair of surfaces so that a single gap geometry could be maintained for the duration of the test run. In order to run the SFA remotely, scripts written with a commercial software package, LabVIEW, were used to control of the SFA components, its FECO-monitoring camera and all its peripheral electronic equipment as well. Samples were agitated to disrupt any shear-induced liquid crystal domain alignment from previous testing following each shear test, and methodologies were developed to ascertain the extent of confinement quickly and remotely following agitation. Separate methods were developed for gap sizes at each extreme of the mesoscale regime, where the transition from bulklike structure and dynamics to nano-confinement occurs (between 1 and 10 microns for smectic-A 8CB).
The results revealed that the greater amplitude-gap aspect ratio and surface-to-domain contact associated with smaller gaps facilitated reorientation of the domains in the shear direction. Evidence was also presented of domains at the higher end or outside of the mesoscale regime that, while straining and accreting, were unable to reorient and thereby led to an overall increase of viscoelastic response. The effective viscosity was found to obey a simple power law with respect to shear rate, , and the flow behaviour indices, n, slightly in excess of unity indicate shear thickening occurs with large enough shear amplitude, and that the viscosity reached a plateau near unity over shear rates of 0.005 to 500 s-1 within the mesoscale regime. Different K and n values were observed depending on the shear amplitude used.
Unlike bulk smectic 8CB, whose domains do not align well in the shear direction with large shear-strain amplitude, at mesoscale levels of confinement large amplitude shearing (up to 12.5 shear strain amplitude) was found to be very effective at aligning domains. In general domain reorientation is found to be much more rapid within the mesoscale regime than has been reported in bulk. Aggressive shearing was found to result in a complete drop in viscoelastic response within seconds, while gentler shearing is found to produce a very gradual increase that persists for more than six hours, with individual shear periods exhibiting frequent and significant deviations from the expected smooth shear path that may be a product of discrete domain reorientations.
From these findings, certain traits of the smectic 8CB domain structures under mesoscale confinement were deduced, including how they respond to shear depending on the level of confinement, and how their reorientation due to shear varies not only with shear rate but also independently with shear amplitude. An equation describing the viscosity change as a function of both shear rate and shear amplitude is proposed. The shear amplitude dependence introduces the notion of shearing beyond the proposed smectic 8CB “viscoelastic limit”, which was shown to exhibit behaviour in accordance with Large Amplitude Oscillatory Shear (LAOS) techniques developed for Fourier Transform rheology. The findings provided an understanding of the behavioural changes that occur as one reduces the level of confinement of smectic materials from bulk to nanoconfinement.
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A Study of the Structure and Dynamics of Smectic 8CB Under Mesoscale ConfinementBenson, James January 2012 (has links)
The structure and dynamics of the smectic-A liquid crystal 8CB (4 cyano-4 octylbiphenyl) when sheared and confined to mesoscale gaps (with crossed cylindrical geometry and mica confining surfaces) were studied using a Surface Forces Apparatus (SFA). Triangular shear patterns with frequencies of 0.01, 0.1, 1.0 and 10 Hz, and amplitudes of 62.5 nm, 625 nm and 6.25 m were applied to samples at gap sizes of 0.5 and 5.0 m. The study was performed at room temperature (20.5C) and at two higher temperatures (22C and 27C). In order to minimize the thermal fluctuations within the test chamber and hence to allow for the rapid re-initialization of test runs, the SFA was modified to allow for quick, precise and remote control of the confining surfaces. The procedure maximized the number of tests that could be undertaken with a single pair of surfaces so that a single gap geometry could be maintained for the duration of the test run. In order to run the SFA remotely, scripts written with a commercial software package, LabVIEW, were used to control of the SFA components, its FECO-monitoring camera and all its peripheral electronic equipment as well. Samples were agitated to disrupt any shear-induced liquid crystal domain alignment from previous testing following each shear test, and methodologies were developed to ascertain the extent of confinement quickly and remotely following agitation. Separate methods were developed for gap sizes at each extreme of the mesoscale regime, where the transition from bulklike structure and dynamics to nano-confinement occurs (between 1 and 10 microns for smectic-A 8CB).
The results revealed that the greater amplitude-gap aspect ratio and surface-to-domain contact associated with smaller gaps facilitated reorientation of the domains in the shear direction. Evidence was also presented of domains at the higher end or outside of the mesoscale regime that, while straining and accreting, were unable to reorient and thereby led to an overall increase of viscoelastic response. The effective viscosity was found to obey a simple power law with respect to shear rate, , and the flow behaviour indices, n, slightly in excess of unity indicate shear thickening occurs with large enough shear amplitude, and that the viscosity reached a plateau near unity over shear rates of 0.005 to 500 s-1 within the mesoscale regime. Different K and n values were observed depending on the shear amplitude used.
Unlike bulk smectic 8CB, whose domains do not align well in the shear direction with large shear-strain amplitude, at mesoscale levels of confinement large amplitude shearing (up to 12.5 shear strain amplitude) was found to be very effective at aligning domains. In general domain reorientation is found to be much more rapid within the mesoscale regime than has been reported in bulk. Aggressive shearing was found to result in a complete drop in viscoelastic response within seconds, while gentler shearing is found to produce a very gradual increase that persists for more than six hours, with individual shear periods exhibiting frequent and significant deviations from the expected smooth shear path that may be a product of discrete domain reorientations.
From these findings, certain traits of the smectic 8CB domain structures under mesoscale confinement were deduced, including how they respond to shear depending on the level of confinement, and how their reorientation due to shear varies not only with shear rate but also independently with shear amplitude. An equation describing the viscosity change as a function of both shear rate and shear amplitude is proposed. The shear amplitude dependence introduces the notion of shearing beyond the proposed smectic 8CB “viscoelastic limit”, which was shown to exhibit behaviour in accordance with Large Amplitude Oscillatory Shear (LAOS) techniques developed for Fourier Transform rheology. The findings provided an understanding of the behavioural changes that occur as one reduces the level of confinement of smectic materials from bulk to nanoconfinement.
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Interactions et propriétés physico-chimiques de surfaces modèles de biomatériauxGiraud, Lucie 12 1900 (has links)
La surface d’un implant ou d’un système à libération contrôlée de médicament est la première zone en contact avec les systèmes physiologiques. Les propriétés de surface vont alors définir le devenir à court et long termes de ces biomatériaux dans l’organisme. Pour améliorer la biointégration mais aussi l’efficacité des matériaux en contact avec les fluides et tissus biologiques, un fin contrôle des phénomènes se produisant à l’interface biologique est nécessaire. Cette thèse s’intéresse à l’étude de trois types de surfaces pouvant modéliser celles de biomatériaux couramment employés.
Dans un premier temps, la stabilité hydrolytique de surface amino-fonctionnalisée a été investiguée. L’amino-fonctionnalisation de surface via l’emploi de monocouche auto-assemblée rencontre un intérêt certain pour l’ancrage de diverses molécules, macromolécules, systèmes colloïdaux et cellules. Cependant, le manque de stabilité en milieu aqueux limite grandement leurs perspectives d’utilisation pour la fonctionnalisation de surface de biomatériaux. Dans ce manuscrit, une monocouche amino-fonctionnalisée à base d’aminoalkylsilane a été greffée sur des substrats de silicate (silice et mica). L’extrême stabilité hydrolytique rapportée pour cette monocouche permet une immersion prolongée en milieu aqueux et sur une large gamme de pH. Les paramètres ayant été identifiés comme impactant cette stabilité sont l’organisation de la monocouche, la densité de greffage et la longueur de la chaîne carbonée de l’aminoalkylsilane.
Dans un second temps, les propriétés lubrifiantes en milieu aqueux de surfaces structurées sont rapportées. Le besoin en surface autolubrifiante couvre une large variété de biomatériaux tels que les substituts cartilagineux, les dispositifs oculaires ou bien les cathéters. Des structures dômes ont été produites sur des surfaces via l’immobilisation de particules. Des particules polymériques à base de polyélectrolytes sensibles aux variations de pH ont permis l’obtention de structures molles et déformables alors que l’immobilisation de particules de silice a permis la formation de structures dures. Deux mécanismes majeurs contrôlant les propriétés de frottement ont été mis en évidence. Les surfaces structurées à partir de polyélectrolytes présentent des propriétés de frottement directement corrélées au gonflement et donc à la teneur en eau de ces structures. Ce
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gonflement peut être contrôlé par le pH du milieu aqueux. Plus les structures sont gonflées, plus le coefficient de frottement est faible. En revanche, avec des structures dures obtenues par l’immobilisation de particules de silice, le roulement de ces particules permet d’obtenir sous certaines conditions des coefficients de frottement extrêmement faibles. Dans ce cas, la nature du lien entre la particule et le substrat importe peu et un dégreffage systématique de certaines particules est observé pour permettre le mouvement des surfaces tout en limitant les forces de frottement.
Dans un troisième temps, la complexation de simples brins de siARN via différentes natures d’interactions a été étudiée à l’aide de surfaces modèles de chimie variable. Cette étude a permis de démontrer la possibilité d'adsorber des simples brins de siARN via des interactions non-électrostatiques sur des surfaces planes. Des interactions hydrophobes et les liaisons hydrogène ont par la suite pu être employées pour complexer cet acide nucléique avec des formulations micellaires et liposomales non-cationiques. Cette étude permet d'envisager la conception de nanovecteurs non-cationiques et donc moins toxiques pour la délivrance de simples brins de siARN.
Les travaux présentés dans ce manuscrit contribuent à l’élargissement des connaissances en matière de propriétés physico-chimiques de surface aux interfaces biologiques. / The surface of an implant or a drug delivery system is the first area of contact with biological environment. The surface properties of these biomaterials will define the short and long term behavior in the organism. To improve biointegration and efficiency, a fine control of the biological interface is required. This thesis investigates three different kind of surfaces modelling commonly used biomaterials.
First, the hydrolytic stability of amino-functionalized surfaces was investigated. The amino-functionalization using self-assembled monolayers is required for the anchorage of molecules, macromolecules, colloidal systems and cells onto biomaterials. However, the lack of stability in aqueous media limits their use. In this manuscript, an amino-functionalized self-assembled monolayer made of aminoalkylsilane was grafted onto silicate substrates (silica and mica). The extreme robustness that we reported for this monolayer allows immersion into aqueous media for a wide range of pH and over long periods of time. The most important parameters that were identified that significantly impact the hydrolytic stability are the order of the monolayers, the grafting density and the length of the alkyl chain of the aminoalkylsilane.
Second, the lubricant properties in aqueous media of structured surfaces are reported. The need in self-lubricant surfaces is required in a wide variety of biomaterials such as the cartilage substitute, ocular medical device or catheters. Domed structures were produced on surfaces through immobilization of particles. Polymeric nanoparticles composed of pH-sensitive polyelectrolytes were used to prepared soft and deformable structures while the immobilization of silica particles allows hard structures to be created. Two main mechanisms controlling friction properties were identified. Friction properties of structured surfaces made of polyelectrolytes were controlled by the swelling and the water content of the particles. This swelling can be tuned by changing the pH of the aqueous media. An increase in particle swelling leads to a decrease in the friction coefficient. However, with the hard structures, the rolling of the particles in some cases can also lead to extremely low friction coefficient. In that case, the nature of the attachment of
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the particle to the surface does not matter and systematic degrafting of some particles was observed which allows surfaces to slide with small friction forces.
Third, the complexation of a single-stranded siRNA through different interactions was investigated with model surfaces of various chemistry. The results show that ss-siRNA can adsorb onto hydrophilic (positively and negatively charged) as well as on hydrophobic substrates suggesting that the complexation can occur through hydrophobic interactions and hydrogen bonding in addition to electrostatic interactions. This study suggests that non-electrostatic interactions could be exploited to complement electrostatic interactions in the design of less toxic nanocarriers and that non-cationics nanovectors can be employed as a potential single-stranded siRNA delivery systems.
The results presented in this thesis contribute to increase the knowledge in the field of physico-chemistry surface properties of biological interfaces.
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