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In the past decade there has been increasing interest in exploring the use of nanocellulose in medicine. However, the influence of the physicochemical properties of nanocellulose on the material´s biocompatibility has not been fully investigated.  In this thesis, thin films of nanocellulose from wood (NFC) and from Cladophora algae (CC) were modified by the addition of charged groups on their surfaces and the influence of these modifications on the material´s physicochemical properties and on cell responses in vitro was studied. The results indicate that the introduction of charged groups on the surface of NFC and CC results in films with decreased surface area, smaller average pore size and a more compact structure compared with the films of unmodified nanocelluloses. Furthermore, the fibres in the carboxyl-modified CC films were uniquely aggregated and aligned, a state which tended to become more prevalent with increased surface-group density. The biocompatibility studies showed that NFC films containing hydroxypropyltrime-thylammonium (HPTMA) groups presented a more cytocompatible surface than unmodified NFC and carboxymethylated NFC regarding human dermal fibroblasts. Carboxymethyl groups resulted in NFC films that promoted inflammation, while HPTMA groups had a passivating effect in terms of inflammatory response.  On the other hand, both modified CC films behaved as inert materials in terms of the inflammatory response of monocytes/macrophages and, under pro-inflammatory stimuli, they suppressed secretion of the pro-inflammatory cytokine TNF-α, with the effects of the carboxylated CC film more pronounced than those of the HPTMA CC material.  Carboxyl CC films showed good cytocompatibility with fibroblasts and osteoblastic cells. However, it was necessary to reach a threshold value in carboxyl-group density to obtain CC films with cytocompatibility comparable to that of commercial tissue culture material.  The studies presented here highlight the ability of the nanocellulose films to modulate cell behaviour and provide a foundation for the design of nanocellulose-based materials that trigger specific cell responses. The bioactivity of nanocellulose may be optimized by careful tuning of the surface properties. The outcomes of this thesis are foreseen to contribute to our fundamental understanding of the biointerface phenomena between cells and nanocellulose as well as to enable engineering of bioinert, bioactive, and bioadaptive materials.

Ce travail étudie les variétés pseudo-riemanniennes compactes localement homogènes à travers le prisme des (G,X)-structures, introduites par Thurston dans son programme de géométrisation. Nous commençons par présenter la problématique générale et discutons notamment du rapport entre la complétude géodésique de ces variétés et une autre notion de complétude propre aux (G,X)-structures. Nous donnons également dans le chapitre 1 une nouvelle preuve d’un théorème de Bromberg et Medina qui classifie les métriques lorentziennes invariantes à gauche sur SL(2,R) dont le flot géodésique est complet. Conjecturalement, toute (G,X)-structure pseudo-riemannienne sur une variété compacte est complète. Nous prouvons ici que cela est vrai pour certaines géométries, sous l’hypothèse que la (G,X)-structure est a priori kleinienne. On en déduit que, pour ces géométries, la complétude est une condition fermée. Lorsque X est un groupe de Lie de rang 1 muni de sa métrique de Killing, ce résultat complète un théorème de Guéritaud–Guichard–Kassel–Wienhard selon lequel la complétude est une condition ouverte. Nous nous tournons ensuite vers l’étude des représentations d’un groupe de surface à valeurs dans les isométries d’une variété riemannienne M complète simplement connexe de courbure sectionnelle inférieure à -1. Étant donnée une telle représentation ρ, nous montrons que l’ensemble des représentations fuchsiennes j telles qu’il existe une application (j,ρ)-équivariante et contractante de H2 dans M est un ouvert non vide et contractile de l’espace de Teichmüller (sauf lorsque ρ est elle-même fuchsienne). Ce résultat nous permet de décrire l’espace des métriques lorentziennes de courbure constante -1 sur un fibré en cercle au-dessus d’une surface compacte. Nous montrons que cet espace possède un nombre fini de composantes connexes classifiées par un invariant que nous appelons longueur de la fibre. Nous prouvons également que le volume total de ces métriques ne dépend que de la topologie du fibré et de la longueur de la fibre. / In this work, we study closed locally homogeneous pseudo-Riemannian manifolds through the notion of (G,X)-structure, introduced by Thurston in his geometrization program. We start by presenting the general problem. In particular, we discuss the link between geodesical completeness of those manifolds and another notion of completeness specific to (G,X)-structures. In chapter 1, we also give a new proof of a theorem by Bromberg and Medina which classifies left invariant Lorentz metrics on SL(2,R) that are geodesically complete. Conjecturally, every pseudo-riemannian (G,X)-structure on a closed manifold is complete. Here we prove that it holds for certain geometries, provided that the (G,X )-structure is a priori Kleinian . This implies that, for such geometries, completeness is a closed condition. When X is a Lie group of rank 1 handled with its Killing metric, this result complements a theorem of Guéritaud–Guichard–Kassel–Wienhard, acording to which completeness is an open condition. We then turn to the study of representations of surface groups into the isometry group of a complete simply connected Riemannian manifold M of curvature less than or equal to -1. Given such a representation ρ, we prove that the set of Fuchsian representations j for which there exists a (j,ρ)-equivariant contracting map from H2 to M is a non-empty open contractible subset of the Teichmüller space (unless ρ itself is Fuchsian). This result allows us to describe the space of Lorentz metrics of constant curvature -1 on a circle bundle over a closed surface. We show that this space has finitely many connected components, classified by an invariant that we call the length of the fiber. We also prove that the total volume of those metrics only depends on the topology of the bundle and on the length of the fiber.