Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography (leaves 178-189).

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Prostaglandin G/H synthase-2 (PGHS-2) is a key rate limiting enzyme in the prostaglandin (PG) biosynthetic pathway, and PG synthesis is required for ovulation in pigs. The objective of this study was to characterize the expression and regulation of PGHS-2 in porcine follicles prior to ovulation. The combination of equine chorionic gonadotropin (eCG; 750 IU) followed by human chorionic gonadotropin (hCG; 500 IU) 72 h later was used to induce ovulation in prepubertal gilts. Previous studies have shown that ovulation is generally induced between 40 and 44 h post-hCG in this model. Ovariectomies were performed at 0, 24, 30, 34 and 38 h post-hCG (n = 4 or 5 animals per time-point), and all follicles larger than 4 mm in diameter were isolated. The regulation of PGHS-1 and PGHS-2 proteins was studied by immunohistochemistry and Western blot analyses, whereas the regulation of PGHS-2 mRNA was studied by Northern blot. PG production was assessed by radioimmunoassay (RIA). (Abstract shortened by UMI.)

Metabolites of arachidonic acid, known as eicosanoids, result from two enzymatic pathways: cyclooxygenase (COX) and lipoxygenase (LOX). Indomethacin (INDO), at a dose level of approximately 10 mg/kg, has been commonly used in studies on the role of prostagiandins (PG) in the porcine ovulatory process since it was believed to be specific in its inhibition of the COX pathway. Other evidence suggests that INDO also has inhibitory effects on the formation of LOX metabolites including 12- and 15-hydroxyeicosatetraenoic acid (HETE). There is also evidence that LOX metabolites may have a role in the ovulatory process of the rat and the pig. In the previous studies on PGs in porcine ovulation, the effect of INDO on LOX metabolites was not examined. It has been shown that progesterone has a role in the formation of eicosanoids in the ovary in the rat and the ewe. The results of this study indicate that an intramuscular injection of INDO, at dose levels as high as 5 mg/kg, reduces ovulation rat and preovulatory follicular fluid concentrations of PGF2alpha without affecting concentrations of 12- and 15-HETE in the gonadotropin-primed pig. Indomethacin, at dose levels as low as 1 mg/kg, reduced ovulation rate and intrafollicular PGF2alpha concentrations but 0.1 mg/kg had no effect on ovulation rate or PGF2alpha. In cultured granulosa and theca interna cells, 10 ng or greater of INDO per 3 x 105 cells reduces PGF2alpha accumulation in the media. Accumulation of 12- and 15-BETE in the media was reduced by 100 ng or greater of INDO per 3 x 10 5 cells. To examine the role of progesterone in the formation of eicosanoids, danazol, an inhibitor of steroidogenesis, was added to cultures of porcine follicular cells. This treatment had no effect on progesterone production by either cell type and, similarly, had no effect on eicosanoid production. (Abstract shortened by UMI.)

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Typescript (photocopy). / Digitized by Kansas Correctional Industries

Includes list of publications by the author. Includes bibliographical references (leaves 103-123) Evaluates the nature of the interactions occurring between semen and cells of the uterus that occur following mating in pigs. Describes a novel ability of porcine seminal plasma to induce dose dependent cell-cell adhesion and mitogenesis amongst peripheral blood lymphocytes in vitro.

Includes list of publications by the author. / Includes bibliographical references (leaves 103-123) / x, 123 leaves : ill.;c 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Evaluates the nature of the interactions occurring between semen and cells of the uterus that occur following mating in pigs. Describes a novel ability of porcine seminal plasma to induce dose dependent cell-cell adhesion and mitogenesis amongst peripheral blood lymphocytes in vitro. / Thesis (Ph.D.)--University of Adelaide, Dept. of Obstetrics and Gynaecology, 1993