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Ground-borne vibration from underground rail vehicles is studied analytically. A previously developed model by J.A.Forrest and H.E.M.Hunt is modified to account for different track and vehicle models. The tunnel is modeled as infinite cylindrical shell surrounded by viscoelastic soil. The track is coupled to the tunnel with supports of complex stiffness. The rails, which are modeled as infinite Euler beams, are supported by discrete sleepers with regular spacing, and railpads with complex stiffness. A modified hysteretic damping model for moving loads is applied to soil. Linearized Hertzian contact spring is included between the wheel and the rail. The solution is obtained in frequency domain using random process theory. Effects of improvements in the model are graphically presented to enable comparison with the previously developed model and measurements from literature.

The main objective of this work is to develop models using multivariable regression and artificial neural network approaches for cost estimation of the construction costs of trackworks of light rail transit and metro projects at the early stages of the construction process in Turkey. These two approaches were applied to a data set of 16 projects by using seventeen parameters available at the early design phase. According to the results of each method, regression analysis estimated the cost of testing samples with an error of 2.32%. On the other hand, artificial neural network estimated the cost with 5.76% error, which is slightly higher than the regression error. As a result, two successful cost estimation models have been developed within the scope of this study. These models can be beneficial while taking the decision in the tender phase of projects that includes trackworks.

The overall objective of this thesis is to assess the financial, technical and social feasibility of investing in modern Automated People Movers (APM) transportation systems, generally known as monorails, in METU campus which presents a unique opportunity to fulfill the modern-day transportation needs of METU campus. This study complements the Presidency Office&rsquo / s long term goal to integrate environmental, social and economic sustainability into the policies, practices and culture of the university and ultimately reduce the consumption of all resources on campus and traffic congestion and accidents. In this context, the consequent cost-benefit effects of the proposed monorail system on campus life were quantified in monetary expressions and the corresponding multi-criteria feasibility assessment including: Break-even Analysis, Cost Effectiveness Assessments and Cost Benefit Analysis have been done successfully. According to these analyses the overall capital cost of system is $46.5 million which covers the 24 months project construction period, and an additional annual operating and maintenance cost of $2 million will span the 30 year project life time. Three different scenarios were proposed for financing the project and relevant break-even points were determined for each of the scenarios. Eventually, it appears that based on the evaluations, constructing such a transit system in METU campus will be cost effective and will certainly enhance the transportation, and will contribute to the institutional improvements and environmental preservation schemes of METU campus.

Eine Übersicht über die Verfahren der Erweiterten Suche (TF,IDF, Stemming, Indexing, Klang von Wörtern) sowie Textkorrektur, dazu deskriptorenbasierte Beschreibung von Dokumenten und Abstracts. Es erfolgt eine Evaluierung dieser Verfahren anhand von ausgewählten XML-Metadaten aus dem MONARCH. Den Abschluß bildet eine Analyse zum Ist-Zustand des MONARCH, bezogen auf Qualität der verwendeten Metadaten und deren Nutzbarkeit für die Erweiterte Suche.

The ability of a neuron to alter its synaptic connections during development is essential to circuit assembly. Synapse remodeling or refinement has been observed in many species and many neuronal circuits, yet the mechanisms defining which neurons undergo remodeling are unclear. Moreover, the molecules that execute the process of remodeling are also obscure. To address this issue, we sought to identify targets of the transcription factor unc-55 COUP-TF, which acts as a cell-specific repressor of synapse remodeling in C. elegans. unc-55 COUP-TF is expressed in VD neurons, where it prevents synapse remodeling. DD neurons can remodel synapses because they do not express unc-55 COUP-TF. Ectopic expression of unc-55 COUP-TF in DD neurons prevents remodeling. We identified the transcription factor Hunchback-like hbl-1 as a target of UNC-55 COUP-TF repression. Differential expression of hbl-1 explains the cell-type specificity of remodeling. hbl-1 is expressed in the DD neurons that are capable of remodeling, and is not expressed in the VD neurons that do not remodel. In unc-55 mutants, hbl-1 expression increases in VD neurons where it promotes ectopic remodeling. Moreover, hbl-1 expression levels bidirectionally regulate the timing of DD remodeling, as increases in hbl-1 cause precocious remodeling while decreases in hbl-1 cause remodeling delays. Finally, hbl-1 coordinates heterochronic microRNA and neuronal activity pathways to regulate the timing of remodeling. Increases or decreases in circuit activity cause increases or decreases in hbl-1 expression, and consequently early or delayed remodeling. Thus, convergent regulation of hbl-1 expression defines a genetic mechanism that patterns activity-dependent synaptic remodeling across cell types and across developmental time. We identified other targets of UNC-55 COUP-TF regulation using gene expression profiling, and implicate some of these factors in the regulation of remodeling using functional genomic screens. Our work suggests roles for conserved networks of transcription factors in the regulation of remodeling. We propose a model in which hbl-1 and other targets of unc-55 COUP-TF transcriptional repression are responsible for regulating synapse remodeling in C. elegans.

O Coup¬TF, αποτελεί ορφανό μέλος της υπεροικογένειας των υποδοχέων των στεροειδών/θυρεοειδών ορμονών και κατέχει κυρίαρχο ρόλο στην ανάπτυξη των εμβρύων όλων των μεταζώων. Στην παρούσα Διατριβή μελετήθηκε η cis¬ ρυθμιστική περιοχή του γονιδίου του, με σκοπό την ένταξή του στο γονιδιακό ρυθμιστικό δίκτυο του εμβρύου του αχινού. Με πειράματα in situ υβριδοποίησης βρέθηκε ότι το γονίδιο PlCoup¬TF εκφράζεται στο στοματικό εξώδερμα του γαστριδίου και στη βλεφαριδωτή ζώνη στον πλουτέα, στο είδος Paracentrotus lividus. Από παλαιότερα πειράματα είχε βρεθεί ότι το τμήμα της ανοδικής περιοχής που εκτείνεται από το -232 ως το ¬532 (τμήμα a), είναι απαραίτητο και επαρκές για την έκφραση του γονιδίου αναφοράς (gfp) στη βλεφαριδωτή ζώνη του πλουτέα. Εντός της περιοχής a ανευρέθησαν τρία πιθανά ρυθμιστικά στοιχεία (¬ 453, ¬432 και ¬377) του γονιδίου PlCoup¬TF, τα οποία αναγνωρίζονται από πρωτεΐνες εμβρυικού πυρηνικού εκχυλίσματος. Στοχευμένες μεταλλάξεις των στοιχείων αυτών, οδήγησαν σε μείωση της έκφρασης του γονιδίου αναφοράς (στοιχείο ¬453) και στην εκτοπική έκφρασή του (στοιχεία ¬432 και ¬377). Περαιτέρω μελέτη των παραγόντων που αναγνωρίζουν τα στοιχεία αυτά, οδήγησε στο συμπέρασμα ότι ο μεταγραφικός παράγοντας PlElk αναγνωρίζει το στοιχείο ¬ 453 και ρυθμίζει θετικά το γονιδίο του PlCoup¬TF και ο μεταγραφικός παράγοντας PlOtx αναγνωρίζει το στοιχείο -377 και καταστέλλει την έκφραση του PlCoup¬TF στο αντιστοματικό εξώδερμα. Τα αποτελέσματα της παρούσης εργασίας οδήγησαν στην ένταξη του γονιδίου PlCoup¬TF και των δύο ρυθμιστών του στο γονιδιακό ρυθμιστικό δίκτυο που καθορίζει τη διαφοροποίηση της βλεφαριδωτής ζώνης εντός του εμβρυικού εξωδέρματος. / Coup­TF, an orphan member of the nuclear receptor super family, has a fundamental role in the development of metazoan embryos. The study of the gene's regulatory circuit in the sea urchin embryo will facilitate the placement of this transcription factor in the well­studied embryonic Gene Regulatory Network (GRN). The Paracentrotus lividus Coup­TF gene (PlCoup­TF) is expressed throughout embryonic development preferentially in the oral ectoderm of the gastrula and the ciliary band of the pluteus stage. Two overlapping λ genomic clones, containing three exons and upstream sequences of PlCoup­TF, were isolated from a genomic library. The transcription initiation site was determined and 5′ deletions and individual segments of a 1930 bp upstream region were placed ahead of a GFP reporter cassette and injected into fertilized P.lividus eggs. Module a (−532 to −232), was necessary and sufficient to confer ciliary band expression to the reporter. Comparison of P.lividus and Strongylocentrotus purpuratusupstream Coup­TF sequences, revealed considerable conservation, but none within module a. 5′ and internal deletions into module a, defined a smaller region that confers ciliary band specific expression. Putative regulatory cis­acting elements (RE1, RE2 and RE3) within module a, were specifically bound by proteins in sea urchin embryonic nuclear extracts. Site­specific mutagenesis of these elements resulted in loss of reporter activity (RE1) or ectopic expression (RE2, RE3). It is proposed that sea urchin transcription factors, which bind these three regulatory sites, are necessary for spatial and quantitative regulation of the PlCoup­TF gene at pluteus stage sea urchin embryos. Additional experiments led us to the conclusion that transcription factor PlElk binds to the ­453 regulatory element and positively regulates PlCoup­TF gene in the ciliary band. Furthermore, PlOtx binds to the ­377 regulatory element and negatively regulates PlCoup­TF gene in the aboral ectoderm. These findings lead to the hierarchical positioning of PlCoup­TF within the embryonic GRN

In this thesis, we aim to study derivations from l^1(Z+) to its dual, l^\infty(Z+). We first characterize them as certain closed subspace of l^1(Z+). Then we present a necessary and sufficient condition, due to M. J. Heath, to make a bounded derivation on l^1(Z+) into l^\infty(Z+), a compact linear operator. After that base on the work in [6], we study weakly compact derivations from l^1(Z+) to its dual. We introduce T-sets and TF-sets and then state their relation with weakly compact operators on l^1(Z+). These results are originally due to Y. Choi and M. J. Heath, but we give simpler proofs. Finally, we will study certain classes of derivations from L^1(R+) to L^\infty(R+), and give an elementary proof that they are always mapped into C0(R+).