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11	Mutantní glykosidasy s vysokou substrátovou specifitou a jejich analýza / Mutant glycosidases with a high substrate specificity and their analysis Nekvasilová, Pavlína January 2019 (has links) β-N-acetylhexosaminidases (EC 3.2.1.52, GH 20) are retaining exo-glycosidases that in vivo cleavage both β-N-acetylglucosamine (GlcNAc) or β-N-acetylgalactosamine (GalNAc) residues fom glycostructures. Under suitable reaction conditions, these enzymes are able to synthesize the glycosidic bond in good yields. Substitution of selected amino acid(s) in the emzyme active site by site-directed mutagenesis may change the enzyme's substrate specificity or suppress the hydrolytic activity of the enzyme in favor of synthesis. The present thesis deals with three mutant β-N-acetylhexosaminidases from Talaromyces flavus, in which the amino acid residues responsible for binding to C-4 hydroxyl of the substrate (Arg218, Glu546) were exchanged for amino acids proposed on the basis of molecular modeling. The effect of introduced single point mutations on substrate specificity of prepared enzymes was studied. Mutant β-N-acetylhexosaminidases were heterologously expressed in Pichia pastoris and characterized. Furthermore, transglycosylation reactions with these enzymes were performed. The prepared carbohydrate products were characterized by NMR.
12	The effect of YakA deficiency in <i>T. marneffei</i> infection of THP-1 and J774 macrophage cell lines Parr, Kayla 23 August 2018 (has links) No description available. Biology Immunology Microbiology Pathology Talaromyces marneffei pathogenic fungi J774 THP-1 pro-inflammatory cytokines TNF IL-1 IL-6 ELISA thermally dimorphic AIDS yakA macrophage cell line mycology

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Mutantní glykosidasy s vysokou substrátovou specifitou a jejich analýza / Mutant glycosidases with a high substrate specificity and their analysis

The effect of YakA deficiency in <i>T. marneffei</i> infection of THP-1 and J774 macrophage cell lines