Speech reception threshold (SRT) tools have been developed to assist in the evaluation of hearing. This study was performed to develop, digitally record, evaluate, and equate Taiwan Mandarin trisyllabic words which can be used to measure the SRT. Eighty-nine commonly used trisyllabic words were chosen and digitally recorded by native male and female speakers. The words were then presented to 20 normally hearing subjects at 14 intensity levels (-10 to 16 dB HL) with 2 dB increments. Psychometric function slopes were calculated using logistic regression. Twenty-eight words with steep psychometric function were selected and digitally adjusted to match the mean subject pure-tone average (5.0 dB HL). A list of 28 trisyllabic words which were relatively homogeneous in audibility and slope were developed. The mean slopes for the 28 selected male and female trisyllabic Taiwan Madarin words were 11.3 %/dB (male talker) and 11.7 %/dB (female talker), respectively. Digital recordings of the psychometrically equivalent trisyllabic words are available on compact disc.

Speech recognition thresholds are used for several clinical purposes, so it is important that they be accurate reflections of hearing ability. Variations in the acoustic signal may artificially decrease threshold scores, and such variations can result from being tested in a second dialect. Thirty-two native Mandarin-speaking subjects (sixteen from mainland China and sixteen from Taiwan) participated in speech recognition threshold testing in both dialects to see whether using non-native dialect test materials resulted in a significantly lower score. In addition, tests were scored by two interpreters, one from each dialect, to see whether the scorer's dialect resulted in a significantly different score. Talker dialect was found to be statistically significant, while scorer dialect was not. Factors explaining these findings, as well as clinical implications, are discussed.

Change detection is currently a topic of great interest to theoretic geographic researchers. The necessity to map, monitor, and model land cover change is also important to a variety of applied fields as varied as urban planning and military intelligence. This research compares five algorithms to map urban land cover change in the greater Las Vegas, Nevada metropolitan area. Landsat Thematic Mapper imagery acquired on May 1990 and May 2000 was used as the primary data. The change detection methods yielded simple maps of change vs. no change. These algorithms included image differencing, image ratioing, image regression, vegetation index differencing, and principal components analysis. Each of these techniques accurately identified areas of land cover with moderate levels of accuracy and produced overall change detection accuracy values between 60% and 76% depending on the method. The highest accuracy was obtained by the image ratioing method using the red spectral band (76%). As expected, the determination of change detection thresholds for each technique was critical to the accuracy produced by the algorithm. Moreover, the type of statistic used in optimizing that threshold was also a significant impacting the final accuracy. The approach of using a set of ground points to calibrate the change detection threshold proved to have significant merit.

Cholera toxin (CT), secreted from Vibrio cholerae, causes a massive fluid and electrolyte efflux in the small intestine that results in life-threatening diarrhea and dehydration which impacts 3-5 million people per year. CT is secreted into the intestinal lumen but acts within the cytosol of intestinal epithelial cells. CT is an AB5 toxin that has a catalytic A1 subunit and a cell binding B subunit. CT moves from the cell surface to the endoplasmic reticulum (ER) by retrograde transport. Much of the toxin is transported to the lysosomes for degradation, but a secondary pool of toxin is diverted to the Golgi apparatus and then to the ER. Here the A1 subunit detaches from the rest of the toxin and enters the cytosol. The disordered conformation of free CTA1 facilitates toxin export to the cytosol by activating a quality control mechanism known as ER-associated degradation. The return to a folded structure in the cytosol allows CTA1 to attain an active conformation for modification of its Gsα target through ADP-ribosylation. This modification locks the protein in an active state which stimulates adenylate cyclase and leads to elevated levels of cAMP. A chloride channel located in the apical enterocyte membrane opens in response to signaling events induced by these elevated cAMP levels. The osmotic movement of water into the intestinal lumen that results from the chloride efflux produces the characteristic profuse watery diarrhea that is seen in intoxicated individuals. The current model of intoxication proposes only one molecule of cytosolic toxin is required to affect host cells, making therapeutic treatment nearly impossible. However, based on emerging evidence, we hypothesize a threshold quantity of toxin must be present within the cytosol of the target cell in order to elicit a cytopathic effect. Using the method of surface plasmon resonance along with toxicity assays, I have, for the first time, directly measured the efficiency of toxin delivery to the cytosol and correlated the levels of cytosolic toxin to toxin iv activity. I have shown CTA1 delivery from the cell surface to the cytosol is an inefficient process with only 2.3 % of the surface bound CTA1 appearing in the cytosol after 2 hours of intoxication. I have also determined and a cytosolic quantity of more than approximately .05ng of cytosolic CTA1 must be reached in order to elicit a cytopathic effect. Furthermore, CTA1 must be continually delivered from the cell surface to the cytosol in order to overcome the constant proteasome-mediated clearance of cytosolic toxin. When toxin delivery to the cytosol was blocked, this allowed the host cell to de-activate Gs, lower cAMP levels, and recover from intoxication. Our work thus indicates it is possible to treat cholera even after the onset of disease. These findings challenge the idea of irreversible cellular toxicity and open the possibility of postintoxication treatment options.

Sparkle is a visual texture which appearance basically consists of very bright spots randomly distributed over a darker background. The effect is more apparent to the observer when one of the contributors (sample, observer, illumination) is moved, meaning it is a dynamic effect. While the characterization and measurement of sparkle has been an important topic of research during the past years, the focus has been solely around the static component of it, the dynamic aspect of sparkle has been left out of the discussion. This is somewhat reasonable since the dynamic attribute bases itself on the perception and measurement of static sparkle. The dynamic aspect of sparkle, or “living sparkle” can be described as the variation of its appearance over a given angular distance. Nonetheless, both effects are without doubt related and perhaps together they provide the key to finding the absolute characterization of sparkle. In this work we aim to dive into both aspects. First, the setup of a goniometric system with a color camera was carried out, obtaining a high spatial and angular resolution. Second, measurements of commercial and self-manufactured sample sets were performed, and an analysis algorithm was developed. The algorithm provided a wide range of parameters for different thresholding methods. These methods were put to test when the results were correlated with the visual assessment of a group of 9 samples under one illumination/observation geometry, obtaining the correlation factors for all methods and parameters. The combination of thresholding method and parameter obtained an R2 > 0.9, implying a strong connection with the visual experience. The threshold applied was always fixed at the same value and the parameter (“S_Values”) represented the brightness of the pixels, indicating that the brightness of the sample is the most influential attribute for the perception of sparkle. Then, this same methodology was applied to the rest of the samples in this study (71 total), for the geometries in between 71.2 and 11.2 degrees in 5 degrees steps. The physical parameters of the self-manufactured samples were also used to test the correctness of the algorithm. The results broadly matched the expected values of intensity and particle size. In addition, the algorithm measured the angular variability of the samples though the angular visibility of the sparkle spots. The wider the visible range, the more invariant the sample, and the less apparent the sparkle effect. This work proposes an innovative way of measuring sparkle in its dynamic and static components, a methodology and measurands to do so are proposed and contrasted to visually assessed samples.

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