Seaweeds of the genus Plocamium are known to produce a variety of halogenated monoterpenes. In addition to their ecological role as feeding deterrents, biological activities reported for these compounds include antibacterial, antialgal, antifungal and anticancer activities. An investigation of the non-polar extracts of the seaweed Plocamium corallorhiza resulted in the isolation of six known halogenated monoterpene compounds, 4-bromo-5-bromomethyl-1-chlorovinyl-2, 5-dichloro-methylcyclohexane (2.68), 1,4,8-tribromo-3 ,7-dichloro-3, 7-dimethyl-1,5-octadiene (2.67), 8-bromo-1 ,3,4,7-tetrachloro-3, 7-dimethyl-1,5-octadiene (2.66), 4,6-dibromo-1,1-dichloro-3,7-dimethyl-2,7-octadiene (2.64), 4,8-dibromo-1,1,7-trichloro-3,7-dimethyl-2,5-octadiene (2.65) and 3,4 ,6,7-tetrachloro-3, 7-dimethyl-1-octene (2.63) as well as eight new compounds, including five halogenated monoterpene aldehydes. The new compounds were identified by 1D and 2D NMR spectroscopic techniques as: 8-Bromo-6,7-dichloro-3,7-dimethyl-octa-2,4-dienal (2.72), 8-Bromo-1,1,2,7-tetrachloro-3,7-dimethyl-octa-3,5-diene (2.70), 4,8-Dichloro-3,7-dimethyl-octa-2,4,6-trienal (2.74), 4-Bromo-8-chloro-3, 7-di methyl-octa-2, 6-dienal (2 76), 8-Bromo-4-chloro-3, 7-dimethyl-octa-2,4 ,6-trienaI (2.75), 4-Bromo-1,3,6,7-tetrachloro-3 ,7-dimethyl-octa-1,4-diene (2.71), 8-Bromo-1,3,4,7-tetrachloro-3,7-dimethyl-octa-1,5-diene (2.69), 4,6-Dibromo-3,7 -dimethyl-octa-2,7-dienal (2.73). All compounds were screened for antimicrobial activity, brine shrimp lethality and cytotoxicity towards oesophageal cancer cells. Compound 2.68 was toxic to brine shrimp larvae at a concentration of 50 μ/mL. It also showed promising activity towards oesophageal cancer cells with an IC₅₀, of 2 μg/mL.

Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, Durban, South Africa, 2017. / Background Seborrheic dermatitis (SD) also known as pityriasis capitis is a common skin condition that affects mainly the scalp, causing scaly patches, red skin and stubborn dandruff (Preedy 2012). It has been reported that dandruff occurs in at least 50% of the world’s adult population and approximately 15% to 20% of the world’s total population (Mia 2016). Recent studies suggest that a scalp specific yeast called Malasseiza globosa appears to be responsible for SD (Zhang, Ran, Xie and Zhang 2013). Seborrheic dermatitis does not affect overall health but it can be uncomfortable and may cause embarrassment and low self-esteem (Del Rosso 2011; Preedy 2012). Anecdotal evidence at Ukuba Nesibindi Homoeopathic Community Clinic (UNHCC) indicated that patients with SD responded positively to Calendula officinalis Ø in combination with Olea europaea (olive oil). Notwithstanding this, there is a dearth of clinical data available to validate the aforesaid patient’s positive response. Hence this study aims to provide clinical evidence to prove or disprove patient’s response to Calendula officinalis Ø in combination with Olea europaea (olive oil). Objective The aim of this double blind randomized controlled study was to determine the efficacy of a topical application comprising Calendula officinalis Ø with Olea europaea in the management of SD of the scalp (dandruff). Material and methods The sample was selected by means of non-probability convenience sampling and consisted of 64 consenting participants between the ages of 18 to 50 years who had read the information letter and met the inclusion criteria. Participants were evenly distributed between the treatment and control groups according to the randomization list (32 participants in each group). The treatment group received Calendula officinalis Ø with Olea europaea and the control group received Olea europaea only. Three participants withdrew from the study resulting in only 61 completing the study, 30 from the control group and 31 from the treatment group. The study was conducted at the Durban University of Technology Homoeopathic Day Clinic (DUTHDC) under the supervision of a qualified and registered homoeopathic clinician. The duration of the study was six weeks with three consultations in total. Consultations took place on day 1, day 22 and day 43. At each consultation the participants were assessed by three individuals – the participant themselves, the researcher, and an independent party (the homoeopathic clinician on duty that day). The assessment tools included the Visual Analogue Scale (VAS) for the researcher and clinician consisting of the following categories: irritation, flaking, greasiness, percentage of the scalp involved and overall impression; and the Patient Perception Questionnaire (PPQ) for the patient consisting of the following categories: irritation, flaking, greasiness, itching and overall impression. This was accompanied by a detailed case history and physical examination performed by the researcher. Results Both the control and treatment groups displayed overall improvement in terms of Patient Perception Questionnaire and Visual Analogue Scale which means that a combination of Olea europeae with Calendula officinalis Ø (treatment group) and Olea europeae only (control group) were effective in the management of SD. There was no statistically significant difference between the effect of a combination of Olea europaea with Calendula officinalis Ø and Olea europaea only. In terms of the VAS and PPQ categories, there was a statistical significance between the groups, with the exception of irritation. Significant differences found were as follows: Flaking (clinician and patient rated p = 0.019) on visit 3 for the control group. Greasiness (clinician and patient rated p = 0.027) on visit 3 for the control group. Greasiness (researcher and patient rated p = 0.012) on visit 2 for the treatment group. Percentage of the scalp involved (researcher and clinician rated p = 0.013) on visit 2 for the treatment group. Overall impression (researcher and patient rated p = 0.026) on visit 2 for the control group. Overall impression (researcher and clinician rated p = 0.026) on visit 3 for the treatment group. Conclusion Both the combination of Olea europaea with Calendula officinalis Ø and Olea europaea only improve SD. Therefore, a topical application comprising Calendula officinalis Ø and Olea europaea is effective in the management of SD of the scalp (dandruff) and therefore permits further investigation. / M

Cyclic dipeptides have been well characterized for their multitude of biological activities, including antimicrobial and anticancer activities. Cyclo(His-Gly) and cyclo(His-Ala) have also recently been shown to possess significant anticancer activity against a range of cell lines, despite the limitations of these two molecules with respect to their physicochemical properties. Low Log P results in poor cell permeability which can often be problematic for drugs with intracellular mechanisms of action. It can also results in poor biodistribution, and theoretical Log P values for cyclo(His-Gly) and cyclo(His-Ala) were extremely low making them ideal candidates for inclusion into a nanoparticulate drug delivery system. The aim of this study was therefore to formulate and evaluate liposome-encapsulated cyclic dipeptides that increase the tumour-suppressive actions of the cyclic dipeptides, while showing a high degree of specificity for tumour cells. While liposomes are relatively simple to prepare, inter batch variation, low encapsulation and poor stability are often problematic in their production and this has lead to very few liposomal products on the market. This study aimed at using a comprehensive statistical methodology in optimizing liposome formulations encapsulating cyclo(His-Gly) and cyclo(His-Ala). Initial screening of potential factors was conducted using a 25-1 fractional factorial design. This design made use of two levels for each of the five factors and abbreviated the design to minimize runs. Although not much information is provided by these types of designs, the design was sufficient in identifying two critical factors that would be studies further in a more robust design. The two factors selected, based on the screening study, were cholesterol and stearylamine content. These two factors were then used in designing a response surface methodology (RSM) design making use of a central composite rotatable vii design (CCRD) at five levels (-1.5, -1, 0, 1, 1.5) for each factor in order to better understand the design space. Various factors influenced the measured responses of encapsulation efficiency, zeta potential, polydispersity index, cellular uptake and leakage, but most notable were the adverse effects of increasing stearylamine levels on encapsulations efficiency and cholesterol levels on leakage for both cyclo(His-Gly) and cyclo(His-Ala) liposomes. Optimized formulations were derived from the data and prepared. Fair correlation between the predicted and measured responses was obtained. The cytotoxic activity of the encapsulated cyclic dipeptides were assessed against HeLa and MCF-7 cells and found to have limited improvement in activity. However, modification of the polyethylene glycol (PEG) grafted to the liposome surface in order to target folate receptors showed good benefit in significantly decreasing the IC50 values recorded in all cells lines tested, particularly low folate HeLa cells with the lowest IC50 being recorded as 0.0962 mM for folate targeted cyclo(His-Ala). The results therefore indicate that hydrophilic cyclic dipeptides are ideal candidates for inclusion into targeted drug delivery systems such as liposomes. Key words: Liposomes, cyclo(His-Gly), cyclo(His-Ala), cyclic dipeptides, HeLa, MCF-7, folate receptors, factorial design, response surface methodology (RSM), central composite rotatable design (CCRD).

There has been relatively little robust research investigating the experience of family therapy from a client’s perspective. Much of the literature fails to make clear their methods for analysing the data, and takes an ‘either or’ approach to family and individual perspectives. Thus, either whole family perspectives, or the perspectives of a particular group are sought, making it difficult to understand the impact of the family context on individual perspectives or vice versa. The present research seeks to understand the family therapy experiences of individuals within their familial context. Two families of three were interviewed using a semi-structured interview guide and interviews were analysed using Interpretative Phenomenological Analysis. Results are presented as two family case studies. One over-arching theme of ‘the safety of the therapeutic relationship’ emerged from the accounts of both families. The therapeutic relationship provided the safety to talk and explore problems and relationships. This was described as cathartic and helped family members to see themselves and each other differently. Varying degrees of exploration of individuals was associated with differing levels of engagement with therapy. Being able to explore relationships for both families allowed them to develop new understandings of each other. Gender also emerged as an important theme and this is discussed in relation to issues of power and gender. Some key methodological limitations of the research including the small number of participants and the impact of an overly detailed interview schedule on the data are discussed. As this study involves two case studies of three family members each, it is not easily transferable, but points to some key themes and processes which have implications for practice and future research.

Previous controlled studies have shown that severe daytime restriction of dietary protein improves the efficacy of L-dopa and reduces response fluctuations in some Parkinson's disease in-patients. The main purpose of the present study was to investigate the nutritional adequacy of the restricted protein diet. Other objectives were to assess the patients' acceptance of the diet and to identify the practical difficulties encountered in following the diet at home. The effect of the restricted protein diet on the subjects' response to levodopa was also examined. Subjects were 11 free-living, otherwise healthy Parkinson's disease patients who suffered from unpredictable response fluctuations to Sinemet (L-dopa containing medicine). This condition is also described as the "on-off" phenomenon. They were counselled to consume a daytime restricted protein diet for 6 weeks (mean ± s.d. protein intake before evening meal 12 ± 2 g for females and 14 ± 3 g for males). Food intake was unrestricted from dinner until bedtime, and subjects were encouraged to consume nutrient-dense foods during this period. The subjects were required to complete a series of three 6-day food records and "on-off" charts (pre-diet, diet week 2, diet week 6). The "on-off" charts indicated the daily number of hours spent in the "on" state (when medication is effective and parkinsonian symptoms are controlled) and in the "off" state (when medication is not effective and symptoms are not well controlled). Hemoglobin, plasma albumin, prealbumin and ferritin were measured before and after the 6-week diet period. Subjective evaluation questionnaires were completed by all participants and their spouses or caregivers. Results from dietary record analysis showed that the restricted protein diet was associated with significant decreases in total intakes of protein, calcium, iron, magnesium, phosphorus, niacin, riboflavin, vitamin B6 and pantothenate, in comparison to "usual" intakes. Intake of energy, carbohydrates, lipids, potassium, thiamin, folacin, and vitamins A, C and B12 did not change significantly. While on the restricted protein diet, only calcium intake was substantially less than the RNI. Biochemical measures of nutritional status were not significantly reduced. Mean body weight tended to decrease (p=.054) over the first 2 weeks and then stabilized until the end of the study period. The results of the "on-off" charts showed that three of the eleven subjects significantly increased their daily time "on" while on the restricted protein diet. Subjectively, six individuals noted an improvement in daytime mobility and indicated that they would maintain the diet for an indefinite period of time. Problems identified by the subjects included hunger prior-'to the evening meal and a lack of variety in food choices. These results show that otherwise healthy and motivated patients with Parkinson's disease can maintain an adequate intake of energy, protein, and most nutrients while on the daytime restricted protein diet. The diet appeared to be relatively well tolerated by patients who obtained a subjective benefit. It is suggested that in patients whose regular diets are marginally adequate, the restricted protein diet might compromise nutrient intakes. Counselling by a registered dietitian is recommended for all patients who undertake this type of diet. / Land and Food Systems, Faculty of / Graduate

M.Tech. (Homoeopathy) / Osteoarthritis (OA) is a chronic and debilitating condition, characterized by irreversible damage to the joint space, most commonly affecting the knees, hips, hands and spine (Colledge et al., 2010). OA is the leading cause of joint pain and disability in middle-aged and elderly persons (Long et al., 2001). The prevalence of OA of the knee in adults living in the United Sates has grown from a reported 21 million in 1990 to a total estimate of 26.9 million in 2005 (CDC, 2011). By the age of 65 years, 80% of the total population has been reported as showing radiographic evidence of OA; while a 20-30% of the total population is symptomatic with radiographic evidence of OA (Doherty et al., 2006). Conventional treatment for OA of the knee is aimed at pain management by use of analgesics and non-steroidal anti-inflammatory drugs (NSAIDs). Some negative effects of these drugs include drug dependency, liver and kidney damage, cardiovascular pathologies, gastric upset and depression. Corticosteroid injections are also used to alleviate chronic inflammation and joint pain, but may lead to further joint destruction (Shamoon and Hochberg, 2000; Mayo Foundation for Medical Education and Research, 2011). OsteoEze Gold™ is a nutraceutical product that contains chondroitin sulphate, glucosamine sulphate, vitamin C and manganese. In combination, the constituents of OsteoEze Gold™ have been shown to be useful in the treatment for OA of the knee (Clegg et al., 2006). In addition, studies have shown that these ingredients prove effective in reducing moderate to severe pain in sufferers of OA of the knee (Vidyasagar et al., 2004). The aim of this study was to determine the effect of OsteoEze Gold™ on the inflammatory marker C-reactive protein (CRP) and quality of life in OA of the knee using blood tests and the Arthritic Impact Measurement Scales (AIMS2SF) respectively. This was a 16-week, double blind, placebo-controlled study using matched pairs according to age, gender and severity of symptoms, and formed part of a group study, with another researcher, who utilized the Intermittent and Constant Osteoarthritis Pain scale (ICOAP) Short Physical Performance Battery (SPPB) and the same sample...

Play can be beneficial and effective for children experiencing the stress, fears, and anxieties associated with illness or death by expressing their feelings into activity (Brown 2018). It can be difficult for children to verbally communicate complex emotions because they usually are unsure of what words to say to express their emotions. Therapeutic play is a great coping mechanism for coping with death because it allows children to express their emotions through play. Professionals working with children would be better equipped to meet children’s needs with a firmer understanding on the ways in which therapeutic play techniques can be incorporated into their practice. The impact therapeutic play makes can also be beneficial for families as it can help the families understand what the children comprehend which can lead to more efficient communication between members of the family. Professionals aim to assist children in reaching optimal outcomes, which in turn has beneficial impacts for the family overall.

The purpose of this study was to explore the various animal assisted interventions mental health professionals incorporate in the therapeutic treatment process, as well as the various therapeutic purposes intended with each technique. Participants were recruited from animal assisted therapy related databases. Participants included professionals who practiced in the mental health field. Thirty one participants qualified for the study. A survey was developed based on information found reviewing literature related to animal assisted therapy. Nineteen animal assisted therapy techniques and ten therapeutic intentions were identified from a review of the literature. Participants were asked to rate on a Likert scale how often they incorporated each technique in their treatment process. Additionally, participants were asked to identify which therapeutic purposes they intended with each technique. Results indicated participants incorporated a variety of animal assisted techniques for various therapeutic intentions. Results indicated seven animal assisted techniques were incorporated by more than 50% of the participants. Building rapport in the therapeutic relationship was the most common therapeutic intention reported with a variety of animal assisted techniques.

The therapeutic relationship has been a cornerstone of the theory and practice of psychotherapy since it first emerged as a healing modality. Research has extensively reported on the therapeutic relationship and its role in the outcomes of psychotherapy. Despite the vast research on the therapeutic relationship, little qualitative exploration has focused on both patient and therapist lived experience. Using a hermeneutic phenomenological qualitative methodology, this study examined the lived experiences of both patients and therapists, within the same therapeutic relationship in a South African context. Three patients and three therapists (i.e. 3 patient-therapist dyads) were interviewed using in-depth semi-structured individual interviews (six interviews in total) to explore their experience of the relationship they developed during therapy, and the meanings that have been made of this. Through thematic analysis, using interpretation to deepen the analysis, several themes and subthemes were identified. Both patients’ and therapists’ lived experiences of the therapeutic relationship in psychodynamic psychotherapy clustered around three major and somewhat similar themes, with various sub-themes. Patients’ lived experiences of the therapeutic relationship centred around the following themes and sub-themes: therapist’s therapeutic approach (holding the patient in mind—knowing them; non-judgmental stance; providing an objective presence— perspective; and a consistent presence), facilitating therapeutic factors (mutuality; and therapist self-disclosure), and process within the relationship (describing the relationship—a lifeline; moment of meeting; change; and the relationship over time). Whilst therapist-participants’ experiences of the therapeutic relationship held somewhat similar themes with subtle differences, namely, patient qualities, facilitating therapeutic factors (holding and containing; theoretical orientation; use of technique; and supportive factors for therapists), and process within the relationship (describing the relationship—intimate yet formal; moment of meeting; change; and the relationship over time). Similarities and differences between patient and therapist experiences are examined that may influence the therapeutic relationship. Conclusions are discussed with a consideration of the limitations of the study as well as implications for future research, practice, and training. / Dissertation (MA)--University of Pretoria, 2019. / Psychology / MA / Unrestricted

Trädgårdsterapi är en behandlingsmetod där deltagarna genomgår ett program baserat på upplevelser och arbete i en terapeutisk trädgård med stöd av ett multidisciplinärt team med syftet att behandla olika sjukdomstillstånd. I Sverige tillämpas trädgårdsterapi främst vid vård av psykisk ohälsa som depression, utmattningssyndrom och schizofreni.  Syftet med detta arbete var att undersöka vilken effekt trädgårdsterapi har visats ha vid olika sjukdomstillstånd. Därför genomfördes en litteraturöversikt med systematisk ansats. Sökningar utfördes i databaserna PubMed, APA Psychinfo samt Swemed+ vilket resulterade i tio experimentella studier. Dessa har kvalitetsgranskats och deras resultat har bedömts och sammanställts. Studierna undersöker: cancer i palliativ vård, schizofreni, demens, stroke, smärta, depression och utmattningssyndrom samt hjärt- och kärlsjukdomar. Många av studierna har metodologiska problem som sänker deras kvalitet och bevisvärde.  Det finns inget stöd för att trädgårdsterapi skulle kunna användas för att öka livskvaliteten i palliativ vård för patienter med cancer. Det har inte heller visats fungera bättre än konventionell behandling vid stroke-rehabilitering eller för att förbättra stämningsläget hos personer med demens. Däremot har trädgårdsterapi visats ha gynnsamma effekter på symptomen vid schizofreni, depression och långvarig smärta samt bidra till rehabilitering av depression och utmattningssyndrom. De positiva effekterna på flera av sjukdomstillstånden visar att behandlingen kan ha potential att öka hälsa och lindra sjukdom. Detta kan motivera till ytterligare studier och en fortsatt utveckling av trädgårdsterapin. / Horticultural Therapy is an activity where the participants are working in and experiencing a therapeutic garden with the support of a multidisciplinary team with the aim to treat various illnesses. In Sweden the method is mainly used to treat mental health problems such as depression, fatigue and schizophrenia. The objective of this paper was to examine the effects of horticultural therapy on different illnesses. To reach the objective a review with a systematic approach was performed. A key word search was made in the databases PubMed, APA Psychinfo and Swemed+ which resulted in ten experimental studies that has been used for analyses in this paper. The quality of the studies has been examined and the results have been evaluated and compiled. The included publications examine palliative care, schizophrenia, dementia, stroke, pain, depression, fatigue and cardiopulmonary diseases. Several of the studies have methodological shortcomings which limits their quality and evidential value.  There is no evidence that horticultural therapy could be used to improve the quality of life in cancer patients in palliative care. Neither has it been shown to perform better than conventional treatments in the rehabilitation of stroke or to improve the mood of persons with dementia.  However, horticultural therapy had significant positive effects on the symptoms of schizophrenia, depression and chronic pain and contributed to the rehabilitation of depression and fatigue. The positive effects indicate that horticultural therapy could potentially contribute to promoting health and treating disease. This could motivate further studies and a continued development of the treatment.