The key character separating the dendrochirotid families Sclerodactylidae (sensu Pawson & Fell, 1965) and the Phyllophoridae (sensu Pawson & Fell, 1965), i.e. entire or undivided radial processes to the calcareous ring in the former and sub-divided processes in the latter, is unjustified since most sclerodactylid species also have subdivided processes. It is here assumed that the basis of elevating the subfamily Sclerodactylinae Panning to family level was established on a misinterpretation or mistranslation of the original diagnosis of this subfamily or a lapsus calumni meaning “plates” instead of “processes”. Panning (1949) categorically states that the processes in the Sclerodactylinae are composed of 3–4 large pieces of calcite and only as an exception they are unbroken. Since Pawson & Fell gave no other distinction between the Sclerodactylidae and the Phyllophoridae, the former is here considered an invalid taxon and its three current subfamilies (Sclerodactylinae, Sclerothyoninae Thandar and Cladolabinae Heding & Panning) are re-assigned to the Phyllophoridae. This family now includes six subfamilies: Cladolabinae, Phyllophorinae Östergren, Sclerodactylinae, Sclerothyoninae, Semperiellinae Heding & Panning and Thyoninae Panning. The diagnosis of the Sclerodactylinae, restricted by Thandar (1989), is now modified to include also those forms whose radial and interradial plates may be slightly sub-divided but still form a short tube. Of the eleven genera placed within this subfamily subsequent to its erection, only ten of these remain. Neothyone Deichmann is a preoccupied name for which Lisacucumis is here proposed as a replacement. Thandar’s (1989) diagnosis of the Thyoninae is here accepted, however, the genus Thorsonia Heding is transferred to the Sclerodactylinae. Of the 66 nominal species which currently stand in the “supergenus” Thyone Jaeger, 10 are transferred to Havelockia Pearson within the Sclerodactylinae, while one species is regarded as a synonym of H. herdmani Pearson. In addition, six species are transferred to Stolus Selenka within the Thyoninae. Finally, three species are transferred to Sclerothyoninae, two within Sclerothyone Thandar and one within Temparena Thandar. Two species show an uncertain affinity to Thyone and are temporally removed from the genus. Furthermore, two species currently classified within Havelockia are transferred to Thyone. The now remaining 46 species are separated into seven groups based on the composition of their introvert deposits: tables only (8 spp.), rosettes only (5 spp.), tables and rosettes (21 spp.), tables and plates/?reduced tables (2 spp.), rosettes and plates/?reduced tables (3 spp.), plates only (2 spp.), or introvert deposits absent or unknown (5 spp.). Regrettably, no other character could be used in conjunction with the above to suggest at least sub-generic levels. Within the genus Havelockia, Cucumaria redimita Sluiter indicates an affinity with Pentamera Ayres. It is here transferred to this genus within the Thyoninae. Havelockia, now containing 17 species, is also revised. Keys, diagnoses and figures are provided for all nominal species now included in Thyone and Havelockia. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2011.

Leopard Panthera pardus are an economically valuable asset and when used in sustainable consumptive use programs can provide tangible benefits to communities to improve human livelihoods and the conservation of the species. Sport hunting is increasingly proposed as a tool to generate funds to support the conservation of leopard and other large carnivores. However, to assess the value of sport hunting as a conservation tool it is critical to understand its economic impact and ensure that the off-takes are sustainable. In this study I assessed the conservation status of leopard and the ecological sustainability of legal and illegal off-take in Niassa National Reserve (NNR) the largest protected area, 42,000 km2, in Mozambique, which is inhabited by 35,000 people. I also investigated whether the revenues from leopard sport hunting off-set the costs of depredation on livestock in local communities and individual benefits from poaching by local hunters. To perform this study, I interviewed hunting operators and villagers, collected camera trapping data, and analyzed long-term leopard sport hunting data. Leopard had high value for sport hunters, however, the economic benefits from the legal hunting did not off-set the costs from livestock depredation and did not compete with benefits from the illegal hunting which accrued to individuals at the household level. Leopard population densities in Niassa Reserve were comparable with the study sites in central and southern Africa. The numbers of leopard legally hunted in NNR appear to be ecologically sustainable, however a high percentage of the leopard taken as trophies were under the recommended age of seven years. The illegal off-take was unsustainable and resulting in high turnover and combined with the operators’ off-take is likely to be negatively affecting leopard populations. For the future ecological and economic sustainability of leopard quotas, I recommend improvements in the distribution of economic benefits and creating economic incentives to encourage villagers not to engage in the illegal hunting and quantification and inclusion of the illegal off-take in the annual quotas. My study also indicates the need to zone community and wildlife areas in NNR to reduce the anthropogenic effects on leopard and other carnivore populations. / Thesis (M.Sc.)-University of KwaZulu-Natal, Westville, 2012.

Considered key landscape elements, ecotones play an important role in landscape ecology. In heavily fragmented, or heterogeneous landscapes, ecotones become a major, even dominant, feature. Yet, there are relatively few studies investigating communities of invertebrates associated with ecotones, especially across natural boundaries. Furthermore, most analyses of habitat loss do not consider the characteristics of the areas surrounding remaining habitat, the matrix. This thesis attempts to partially fill the gap. Afromontane forest-grassland ecotones are characteristically sharp (usually a few metres), are mainly fire-maintained, and have been in existence for, perhaps, millions of years. Therefore they provide a good opportunity to study ecotone, forest patch and grassland matrix characteristics together, and the associated species assemblages. In short, I investigated the diversity and distribution patterns of epigaeic invertebrates across ecotones between the natural and isolated patches of Afromontane forests and the surrounding natural grassland matrix, in the province of KwaZulu-Natal, South Africa. In particular, the following primary hypothesis was evaluated; do edge effects, in terms of elevated abundance and species richness, and in terms of abrupt, significant changes in environmental conditions, occur across near-natural ecotones. These edge effects quite often occur across anthropogenically-created habitat junctions, but it is not clear whether they do across natural ones. To test this hypothesis, the following secondary hypotheses were evaluated. Firstly, often a single invertebrate taxon is used for assessing changing landscape patterns. However, recent work has suggested that patterns and responses vary widely between taxa, and that management programmes based on the knowledge of a single taxon would not necessarily predict or safeguard that of others. Therefore, in chapter I, the hypothesis whether a single taxon could be used in biodiversity studies, or alternatively, whether it is better to select an array of taxa, was tested. Several invertebrate taxa were selected to investigate this. These included terrestrial amphipods, spiders, carabids, staphylinids and ants. Indeed, results showed that species diversities and assemblage-compositions of epigaeic spiders, carabids, staphylinids and ants were significantly different in different-sized Afromontane forest patches. Only carabids and staphylinids correlated positively with each other in terms of numbers of species. The other taxa showed only weak positive, or negative, correlations in their species richness. Results supported the multi-taxa approach in conservation studies, even among groups sharing a common habitat stratum. Secondly, organismal diversity usually increases at disturbed habitat edges. This phenomenon is commonly referred to as the biological edge effect. This pattern, however, is not universal and a number of authors have shown evidence contradicting this hypothesis. In chapter II amphipods, ground beetles and ants were collected to test the biological edge effect hypothesis. In addition, a number of abiotic factors were measured across these forest-grassland boundaries in an attempt to relate the biotic with the abiotic. Little evidence was found to support the classical edge-effect hypothesis (elevated species richness at the ecotone). In fact, carabid abundance and species richness was high in forests, decreasing gradually through the ecotone to a low in grasslands. In contrast, ant species richness increased significantly from a low in forests, increasing gradually through the ecotone, to a high in grasslands. Certain species did, however, show a significant increase in abundance at the ecotone, such as Talistroides africana, a terrestrial amphipod, and Tetramorium avium, a seed-predatory ant. Afromontane forest-grassland ecotones are natural and are not the result of anthropogenic clear-cut fragmentation. They also lacked any great changes in micro-environmental conditions. I hypothesise that edge effects are of less importance at more naturally maintained habitat boundaries even if these boundaries are sharp. Thirdly, climatic variation has a major impact on invertebrate communities. The Afromontane landscape experiences hot and wet summers, and cool and dry winters. I hypothesised that invertebrate distribution patterns across an ecotone change from one season to the next (chapter III). For example, it is expected that certain winter-active species might disperse from one location along the gradient to another, perhaps to escape predators, or find winter-refugia. This would, in turn, change the pattern of distribution of the selected taxa across these ecotones. Surprisingly, the general pattern of distribution across these ecotones changed little. However, there were significant differences between summer, spring, winter and autumn catch, and species identities changed from one season to the next. For example, carabid abundance and species richness was higher in the forest, compared to in the grassland, while ants were species richer in the grassland, compared to in the forest. These patterns were consistent from one season to the next. Again, as was found in chapter II, T. africana was significantly more abundant at the ecotone, compared to either forest or grassland interiors, in all seasons throughout the year. To summarise, amphipods favoured the ecotone environment, carabids the forests and ants the grasslands, throughout the year. Finally, scientists have recently become aware of the importance of the matrix surrounding habitat patches, in the survival and occurrence of organisms in the habitat patch. I tested whether the quality of the matrix, as a function of human disturbance, has an influence on invertebrate occurrence and distribution patterns across Afromontane forest-grassland boundaries. Redgrass (Themeda triandra Forssk.) dominated Afromontane grasslands are, and have been experiencing varying degrees of anthropogenic disturbance. Consequently, ecotones vary from being very abrupt (heavy disturbance in the matrix) to gradual (little disturbance in the matrix), although still sharp compared to most ecotones elsewhere. Level of grassland disturbance influenced amphipod, carabid and ant assemblage-structure across Afromontane ecotones (chapter IV). Results support the hypothesis that the dynamics of remnant areas are influenced by factors arising in the surrounding landscape. In particular, carabid assemblage-composition changed highly significantly from undisturbed to disturbed sites (this taxon was mainly captured from forests). Furthermore, only a single carabid individual was captured from 8 to 128 m into the grassland and only 14 T. africana individuals were captured from 2 m inside the forest to 128 m into the grassland of the most disturbed site. Clearly, matrix quality influenced not only the patterns of occurrence of organisms in the grassland matrix, but also at the ecotones and in the forest patches. In conclusion, it is imperative to investigate a number of taxonomic groups in conservation ecology to give more reliable results, and thus conservation recommendations. Of course, not all taxa can be considered, and the selection of appropriate taxa still poses a problem, but a set of taxa that are considerably different biologically is a good start. Native Afromontane forest-grassland mosaics are in urgent need of conservation, as much of this habitat is subject to heavy anthropogenic disturbance such as human settlement, forestry, cattle grazing, agriculture, and frequent, out of season, fires. Unfortunately only 2% of this biome is protected in South Africa. Furthermore, matrix quality is important because it determines the survival rate of propagules moving between remnant patches of habitat, and therefore the success rate of such movements. Private land-owners own most of this grassland area in the Afromontane region, but no guidelines are available to them on how to protect this habitat. Throughout this thesis I emphasised the importance of protecting both native forest and the surrounding native grassland. A first initiative is simply to protect a zone of grassland around the forest patches, both in terms of less frequent burning regimes, and less, or no cattle grazing here. By doing so, a rich grassland ant fauna will be conserved, the grassland matrix quality will improve, and a rich carabid fauna, favouring predominantly Afromontane forest remnants, will be conserved. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 2000.

The primary aim of this study was to investigate the role of food supply and ground cover on the community structure, population dynamics and demography of terrestrial small mammals in a subtropical grassland. This aim was achieved through a series of food supplementation and cover manipulation experiments conducted at eKundizeni Farm near Matsapha, Swaziland, over a 28 month period. The effects of five different diets on the rodent Mastomys natalensis were investigated in the laboratory, and the results showed that rolled oats and rabbit pellets were suitable for growth and reproduction in this species. The effects of supplementary food were investigated on two supplemented grids and one control over a twelve month period. Small mammal biomass increased significantly on the supplemented grids in relation to the control. This increase in biomass was the result of a twofold increase in the numbers of M natalensis. Food supplementation further affected M. natalensis by: extending the breeding season of females; increasing body weight; increasing survival; and decreasing home range area. Food supplementation had a weak positive effect on the density of another rodent Lemniscomys rosalia, but did not affect any other demographic feature of this species. Food supplementation did not have a demographic effect on any other species of small mammal captured. The effects of vegetative cover were investigated, over a twelve month period, on two control grids and four manipulated grids on which the vegetative cover was mechanically reduced. Supplementary food was added to two of the latter four manipulated grids. The biomass of small mammals, including M. natalensis, was lower on grids with reduced vegetative cover than on the controls. However, food supplementation resulted in a significant increase in the biomass of M. natalensis on one of the manipulated grids. Hence, M. natalensis was induced to shift to a habitat with reduced cover by the provision of supplementary food. Additional information on the population dynamics, age structure, reproduction and diet of M natalensis, L. rosalia, Mus minutoides and Steatomys pratensis is also presented. / Thesis (Ph.D.)-University of Natal, Pietermaritzburg, 1997.

Our understanding of bird pollination systems has changed dramatically in the last few years. A long-standing paradigm was that hummingbirds and passerine birds select for different nectar properties in flowers (phylogenetic hypothesis). However, specialist passerines, such as sunbirds, have similar nectar preferences to hummingbirds and nectar in plants pollinated by these two bird groups is strongly convergent. Thus, as an alternative to the existing paradigm, it has been argued that the most useful distinction that can be drawn is that between specialist and generalist avian nectarivores (feeding niche hypothesis). This was supported by phylogenetically-controlled analyses that show that nectar in plants pollinated by specialist avian nectarivores (whether hummingbird or passerine) tends to have a lower volume, higher concentration and higher sucrose content than that in plants pollinated by generalist avian nectarivores. The aim of this thesis was to determine if these trends can be explained by the nectar preferences of avian nectarivores, and to determine whether the generalist-specialist dichotomy can be applied to Kniphofia, a largely bird-pollinated African plant genus. This thesis consists of two sections. In the first, I present data from equicaloric choice experiments to determine sugar preferences of both specialist and occasional nectar feeding birds across a range of concentrations. In addition, I determine apparent sugar assimilation efficiencies and concentration preferences for a range of occasional nectar feeding birds. Using Malachite Sunbirds (Nectarinia famosa), Dark-capped bulbuls (Pycnonotus tricolor), Speckled Mousebirds (Colius striatus) and Red-winged Starlings (Onychognathus morio) (plus Village Weavers (Ploceus cucullatus) in an appendix) as representatives of the major groups of nectar feeding birds in South Africa, I show that some differences occur between occasional nectar feeding birds and all specialist nectar feeding birds studied so far. Specialists show a switch from hexose preference at low (5%) concentrations, exhibit no preference at medium concentrations (10-20%) and prefer sucrose or show no preference at high concentrations (25%). However, specialists are unable to maintain energy balance at low concentrations, and always select the higher concentration when given choices within this range. Occasional nectar feeding birds prefer hexose solutions at low and medium concentrations (5 and 10%), but show varied preferences at higher concentrations. Occasional nectar feeding birds are able to maintain energy balance at low concentrations, and either show no concentration preference, or a preference for lower concentrations when given a choice. Occasional nectar feeding birds show a range in ability to digest sucrose, although some species, like the Dark-capped Bulbul, are quite proficient at it. Surprisingly, no significant difference is found between specialist and occasional nectarivores sugar preferences when analysed globally, even when phyllogeny is accounted for. Instead, I found a significant relationship between body size and bird sugar preference at different concentrations, from which I build the body size hypothesis, which I suggest is a better predictor to use than bird diet type (specialist or occasional nectarivores – feeding niche hypothesis). In the second section of the thesis, I examine the associations between plant traits and nectarivore nectar preferences. This section focuses on flower morphology and nectar characteristics in Kniphofia species, and ecotypes within species, pollinated by specialist versus occasional avian nectarivores. I show that apart from sugar type, which appears to be phylogenetically constrained, flower morphology and nectar characteristics appear to diverge according to whether plants are pollinated by generalist or specialist nectar feeding birds. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2009.

Urbanisation through the process of habitat loss and fragmentation has caused drastic changes in ecosystem dynamics around the world. Many species can no longer survive in these urban areas; however there are those species that have been able to survive and in fact thrive in the newly created habitats. With increasing urbanisation it is important that animals are able to adjust to a life in close association with humans. One such group of organisms which has adjusted well to urbanisation is the suborder Megachiroptera (Chiroptera). Some species from this suborder have benefited from increased food and roost resources in certain urban areas. Exotic fruiting plants (introduced purposely and accidentally) as well as increased cultivated gardens have provided additional food sources in some urban environments, while man-made structures, and increased suitable vegetation, have provided additional roosting opportunities. Although these urban dwelling species live in close association with humans, very little is known of their ecology in urban areas. Wahlberg's epauletted fruit bat Epomophorus wahlbergi is one such species of which little is known of its suburban ecology despite its increased presence in many urban areas. This study on the ecology of E. wahlbergi was conducted from February to October 2011 in the urban environment of Pietermaritzburg, South Africa. The aim was to examine foraging movements and habitat use of E. wahlbergi in this urban environment. The objectives were to determine seasonal differences in foraging movements and home range sizes in this urban environment. In addition the roosting dynamics and roost characteristics of E. wahlbergi in this urban environment were determined. In late summer, it was found that individual E. wahlbergi movements ranged considerably, with some bats making extensive flights to different parts of town while others stayed in particular areas throughout; no bats were recorded to have left the urban environment. Some of the larger distances covered in a single night's movements were two and five km. In late summer roosting fidelity varied between individual bats; all the individual bats changed their roosts at least once during late summer. Some individuals had as many as three known daytime roost sites. There was a difference in home range size between the sexes; with females occupying a larger home range size than males. This variation in movement patterns of individual bats suggests that their social interactions, roost site preferences, or dietary preferences vary between individuals in late summer. A significant difference in home range size and habitat use by E. wahlbergi was found between winter and spring, with home range sizes being larger in winter. The increased home range sizes and habitat use in winter were a consequence of bats feeding on the fruits of the alien invasive Syringa (Melia azedarch) with few other trees in fruit. Consequently bats had to move greater distances for food in winter. In spring, fruit availability was greater and more varied including both indigenous and exotic fruits. Consequently in winter, the bats were more reliant on a few fruiting species to meet their dietary requirements than during spring. Bats changed their roosts regularly in summer, winter and spring. There was considerable variation in roost temperatures however roost temperatures were higher than ambient temperatures. Roosts in man-made structures were higher in temperature than those in natural vegetation. This study suggests the importance of temperature in the selection of daytime roosts, however other factors such as predator avoidance and proximity to food resources are also considered in selection of daytime roosts. Within the order Chiroptera, species from the suborder Microchiroptera have generally not been well represented in urban areas, it is important that the reasons for this be better understood. Further research is still required to better understand the ecology of urban dwelling species as well as to understand the reasons why many species are not able to adjust to urban environments. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

Although sleep forms an important part of an animal’s life, there is a paucity of knowledge about sleep behaviour. The function of sleep in birds is poorly understood, even though birds spend a large part of their lives sleeping. Sleep behaviour in passerine birds has not been looked at as extensively as that of non-passerine birds. I looked at the sleep behaviour of three relatively common passerine birds occurring in southern Africa, namely the Malachite Sunbird (Nectarinia famosa), the Cape White-eye (Zosterops pallidus) and the Fan-tailed Widowbird (Euplectes axillaris). By using an infra-red sensitive camera I described basic sleep behaviours at various ambient temperatures, of all three species, such as sleep position and eye closure, and also investigated the incidence of unihemispheric sleep. Individuals of all three species spent most of the night asleep and kept on waking up intermittently throughout the night, with no significant differences between temperatures. Cape White-eyes and Malachite Sunbirds showed an increase in back sleep and a decrease in front sleep at 5oC. Little evidence of unihemispheric sleep was found, suggesting that it is more likely to occur in non-passerines, especially ground dwelling birds. Diurnal birds generally sleep during the hours of darkness. Most male southern African sunbirds have pectoral tufts, although the function of these is not always understood. In male Malachite Sunbirds it has recently been found that they display their pectoral tufts almost continuously throughout the night, whilst asleep. I explored the possible function of this behaviour and suggest that these tufts might be a deterrent to predators, as they look like ‘eyes’ in the dark. A review of the use and occurrence of pectoral tufts in southern African sunbird species is also presented. Blood glucose concentrations of most birds are much higher than those found in mammals and it is still not known how they evade the complications of such high levels. I investigated the change in blood glucose concentrations of Malachite Sunbirds at two different ambient temperatures and at different times of the night and day and explored the possibility that gluconeogenesis might be used by birds to ‘warm up’ during arousal of torpor in the early morning, before daylight. Generally blood glucose levels were fairly high, between 13.6 and 21.4 mmol/L, which was expected. Blood glucose levels were higher at 5oC than at 25oC and generally lower in the early hours of the morning. Therefore I reject the assumption that Malachite Sunbirds use gluconeogenesis as an additional form of heat generation during torpor. It is thought that the difference in the levels of blood glucose might be a function of the cold temperature and the consumption of their nectarivorous diet. This research clearly highlights the need for further studies to be undertaken in the sleeping behaviours and patterns of birds, especially in southern African species. It also shows that more studies need to be done on the use of pectoral tufts in sunbird species and furthermore it is suggested that more research is needed to elucidate the mechanism by which Malachite Sunbirds are able to rapidly ‘warm up’ during arousal, when in torpor. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.

In this study I investigated patterns of invertebrate diversity in Limpopo Province indigenous forests, in order to highlight forests and taxa of special conservation significance. Invertebrates from seven target taxa were sampled in 11 patches of indigenous forest in Limpopo Province from February 2001 to January 2002, including six forests in the Soutpansberg and five forests in the northern Drakensberg. Selected forests comprise three distinct vegetation subtypes and the target taxa selected were millipedes, centipedes, earthworms, terrestrial molluscs, spiders, scorpions and amphipods. Invertebrates were sampled by active searching of quadrats and line transects and pitfall traps. A total of 11 969 indigenous target group individuals were sampled, comprising 14 orders, 50 families, 86 genera and 142 species (including at least nine new). There was a significant difference in the total invertebrate species richness and diversity of forest patches but results varied considerably when different target group figures were analyzed. With the exception of spiders, the factors influencing total and individual target group richness in forests could not be determined. Introduced invertebrates comprised a large proportion of the species and individuals sampled, but were not shown to affect indigenous fauna. Invertebrate species assemblages were most similar between forests sharing the same vegetation subtype and between forests in the same mountain region. However, each forest patch had unique species and some even had unique families. Limpopo Province forests support high numbers of endemic invertebrates. A total of 47 endemic invertebrate species were sampled, including six site endemics, eight local endemics, nine regional endemics and 24 national endemics. The numbers and scales of endemism varied by target group. Invertebrate species’ distributions in Limpopo Province forests generally support the biogeographic theories of Pleistocene forest refugia and the Limpopo River valley as a radiation barrier, although some important contradictions were found. Local endemism in Limpopo Province forests is likely the product of historical processes. Although some significant relationships were found between surrogate and true measures, single taxon biodiversity indicators, the higher taxon method, morphospecies and land classes could not accurately predict patterns of target invertebrate species richness in Limpopo Province forests. Results show that formal species identification should be used if accurate richness estimates are desired; the use of surrogates is not supported by this study. Conservation of Limpopo Province forests is vital for the preservation of valuable invertebrate communities. No forest sampled in this study can be considered unimportant. Effective forest conservation and management is dependent upon the protection of forests of varying patch size, careful evaluation and control of utilization and the establishment and maintenance of corridors linking isolated forest patches. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2004.

Thesis (MSc)--Stellenbosch University, 2011. / ENGLISH ABSTRACT: With growing concerns about current environmental issues, such as climate change, that affect ecosystems around the world, understanding ecosystem function is becoming increasingly important. In this study, I investigate the plant – seed disperser mutualism between an endemic scatter-hoarding mouse Acomys subspinosus and its Proteaceae plant counterpart Leucadendron sessile in the biodiversity hotspot of the Cape Floristic Region, South Africa. The main objective of this thesis is to investigate the seed selection and caching behaviour of A. subspinosus. First, I determined the seed selection strategy for dispersal and burial by A. subspinosus. Acomys subspinosus may exert stabilizing selection pressure onto L. sessile seeds by dispersing and burying medium seeds with medium hull-thicknesses. Small seeds were eaten in situ and large seeds left at depots. I concluded that the buried L. sessile seeds may have a competitive advantage when it comes to seedling establishment in a post-fire environment, since seeds dispersed by rodents in the fynbos, such as L. sessile, are much larger in size and therefore have more stored nutrients and rapid growth capabilities than seeds dispersed by other vectors. Secondly, I documented rodent dispersal behaviour over a full years’ time. Acomys subspinosus dispersal behaviour changed significantly over the seasons, which I attributed to a change in food availability as the year progressed. Acomys subspinosus buried seeds in autumn after mass seed drop but began to recover caches and consume seeds during winter and spring. The rodent switched to an insectivorous diet in spring. I propose that the A. subspinosus – L. sessile relationship is mutualistic during the year, but the relationship does shift in the favour of the rodent during winter and spring. Lastly, I address the scatter-hoarding behaviour of A. subspinosus and cache recovery ability of its assumed closest food competitor Rhabdomys pumilio. I found that cache size has a profound influence on pilferage rates of L. sessile seeds. Acomys subspinosus scatter-hoarded the majority of seeds singly in the field and R. pumilio had difficulties finding those single-cached seeds in dry substrate under controlled experimental conditions, serving as evidence that scatter-hoarding is an effective method of pilferage mitigation by A. subspinosus during the dry summer months. Relatively little was known about this plant – disperser mutualism and how it functions before this thesis were conducted. I have provided insights into the influence of rodent disperser behaviour on seed morphology development, seed fate and seed persistence in the field and suspect that the plant –disperser relationship may have a larger influence on ecosystem dynamics than previously anticipated. Further research on this system is of importance, especially with today’s emerging environmental instability and human interference that threaten the robustness of highly interconnected ecosystems like the fynbos. / AFRIKAANSE OPSOMMING: Met die huidige omgewingskwessies, soos die klimaatsverandering, wat ekosisteme wêreldwyd affekteer, word die begrip van ekosisteemfunksionering toenemend belangrik. In hierdie studie ondersoek ek die dier – saadverspreidingsmutualisme tussen die endemiese verstrooiings-storing muis Acomys subspinosus en sy Proteaceae plant eweknie Leucadendron sessile in die biodiversiteit 'hotspot’ van die Kaapse Floristiese Ryk, Suid-Afrika. Die hoof doelwit van die tesis is om die saadseleksie en storingsgedrag van A. subspinosus te ondersoek. Eerstens het ek die saadseleksie strategie vir die verspreiding en begrawing deur A. subspinosus bepaal. Acomys subspinosus het direksionele druk uitgeoefen op L. sessile sade deur mediumgrootte sade met medium saadhuiddiktes te versprei en te begrawe. Klein sade was in situ geëet en groot sade was gelaat by afgesette plekke. Ek het die gevolgtrekking gemaak dat die L. sessile sade wat begrawe is ‘n kompeterende voordeel mag hê wanneer dit kom by die vestiging van saailinge in ‘n afgebrande omgewing, aangesien sade wat in die fynbos deur knaagdiere versprei word, soos L. sessile, baie groter is en dus meer gestoorde voedingstowwe en spoedige groeivermoëns het, as sade wat deur ander vektore versprei word. Tweedens het ek die knaagdier verspreidingsgedrag oor die tydperk van ‘n jaar gedokumenteer. Acomys subspinosus se verspreidingsgedrag het beduidend verander deur die verloop van die jaar, wat ek toegeskryf het aan die verandering in voedselbesbikbaarheid soos wat die jaar gevorder het. Acomys subspinosus het sade begrawe in die herfs na grootskaalse vrylating en val van die sade, maar het gestoorde sade begin terug kry en sade begin eet gedurende die winter en lente. Die knaagdier het na ‘n insekvretende dieët omgeskakel in die lente. Ek stel voor dat die A. Supspinosus – L. sessile verhouding nie die hele jaar mutualisties is nie, maar eerder antagonisties, in die knaagdier se guns, gedurende die winter en lente. In die laaste hoofstuk spreek ek die verstrooiings-storingsgedrag van A. subspinosus en storingsverkrygingvermoeë van sy naaste voedselmededinger en deponeringsdief Rhabdomys pumilio aan. Ek het gevind dat die storingsgrootte ‘n beduidende invloed het op die koers van diefstal van L. sessile sade. Acomys subspinosus het die meerderheid van die sade gestoor in die veld en R. pumilio het die enkel-gestoorde sade in droeë substraat onder gekontroleerde eksperimentele kondisies moeiliker gevind. Dit is ondersteunende bewyse dat verstrooings-storingsgedrag ‘n effektiewe metode is om diefstal te verminder in die droë somer in die fynbos. Relatief min was bekend oor hierdie dier – saad verspreidingsmutualisme en hoe dit funksioneer voordat die studie uitgevoer was. Ek het insig verskaf oor die invloed van knaagdier verspreidingsgedrag op saadmorfologie ontwikkeling, die lot van sade en die tydperk wat dit begrawe is in die veld. Ek vermoed dat die mutualisme ‘n hoeksteenproses is in die fynbos en die invloed daarvan op ekosisteemdinamieka mag dalk groter wees as wat voorheen verwag was. Verdere navorsing oor hierdie sisteem is belangrik, veral met vandag se opkomende omgewingsonstabiliteit en menslike inmenging wat die robuustheid van hoogs verbonde-netwerk ekosisteme soos die fynbos bedreig.

Thesis (PhD)--Stellenbosch University, 2008. / ENGLISH ABSTRACT: The order Macroscelidea has a strict African distribution and consists of two extant subfamilies, Rhynchocyoninae with a single genus that includes three species, and the Macroscelidinae represented by the remaining three genera, Elephantulus that includes 10 species, and the monotypic Macroscelides and Petrodromus. On the basis of molecular, cytogenetic and morphological evidence, Elephantulus edwardii (Cape rock elephant-shrew), the only strictly South African endemic species, was shown to comprise two closely related taxa. A new Elephantulus taxon, described here is reported for the first time. It has a restricted distribution in the central Nama Karoo of South Africa. Apart from important genetic distinctions, Elephantulus sp. nov. has several relatively subtle morphological characters that separate it from E. edwardii. Molecular sequences from the mitochondrial cytochrome b gene and the control region of E. edwardii sensu stricto suggests the presence of a northern Namaqua and central Fynbos clade with four evolutionary lineages identified within the latter. The geographic delimitation of the northern and central clades corresponds closely with patterns reported for other rock dwelling vertebrate species indicating a shared biogeographic history for saxicolous taxa in South Africa. Elephantulus rupestris (western rock elephant-shrew) and Macroscelides proboscideus (round-eared elephant-shrew) are two taxa with largely overlapping distributions that span the semi-arid regions of South Africa and Namibia. Based on mitochondrial DNA sequence data E. rupestris has a structured genetic profile associated with a habitat of rocky outcrops compared to M. proboscideus that inhabits gravel plains, where the pattern is one of isolationby– distance. Chromosomal changes, apart from heterochromatic differences, are limited to variation in diploid number among elephant-shrew species. These range from 2n=26 (E. edwardii; E. rupestris; Elephantulus sp. nov.; E. intufi; E. brachyrhynchus and M. proboscideus) to 2n=28 in both Petrodromus tetradactylus and E. rozeti to 2n=30 in E. myurus. Cross-species chromosome painting (Zoo-Fluorescence in situ hybridization or zoo-FISH) of E. edwardii flow-sorted probes that correspond to the five smaller sized autosomes (8-12) and the X chromosome showed no evidence of synteny disruption among Elephantulus sp. nov., E. intufi, E. myurus, P. tetradactylus and M. proboscideus, and reinforced the G-banding observations underscoring the conservative karyotypes in these species. A comprehensive phylogeny including all described elephant-shrew species is presented for the first time. A multigene supermatrix that included 3905 bp from three mitochondrial (12S rRNA, valine tRNA, 16S rRNA) and two nuclear segments (Von Willebrand factor [vWF] and exon 1 of the interphotoreceptor retinoid binding protein [IRBP]) was analysed. Cytogenetic characters, previously described morphological, anatomical and dental features as well as allozyme data and penis morphology were evaluated and mapped to the molecular topology. The molecular findings did not support a monophyletic origin for the genus Elephantulus and suggests that both the monotypic Petrodromus and Macroscelides should be included in Elephantulus. Molecular dating suggests that an arid-adapted Macroscelidinae lineage dispersed from east Africa at ~11.5 million years ago via the African arid corridor to southwestern Africa. Subsequent speciation events within the Macroscelidinae are coincidental with three major periods of aridification of the African continent. / AFRIKAANSE OPSOMMING: Die orde Macroscelidea het ’n verspreiding beperk tot Afrika en sluit twee bestaande subfamilies in, die Rhynchocyoninae wat drie spesies binne ’n enkele genus insluit en die Macroscelidinae verteenwoordig deur drie genera, Elephantulus (10 spesies) en die monotipiese Macroscelides en Petrodromus. Gebaseer op molekulêre, sitogenetiese en morfologiese bewyse, bestaan E. edwardii, tot op datum die enigste streng endemiese Suid- Afrikaanse klaasneusspesie, uit twee nabyverwante taksa. Die nuwe Elephantulus takson, hierin beskryf, het ’n beperkte verspreiding in die sentraal Nama Karoo van Suid-Afrika. Afgesien van belangrike genetiese bewyse wat die beskrywing van die nuwe spesie ondersteun, word Elephantulus sp. nov. gekenmerk deur ’n aantal subtiele morfologiese karakters wat dit onderskei van E. edwardii. Binne E. edwardii sensu stricto, het mitochondriale molekulêre volgordes beduidende substruktuur aangedui regoor die spesies se verspreiding. Die data het die teenwoordigheid van ’n noordelike Namakwa en sentrale Fynbos klade aangetoon met vier evolusionêre lyne binne die laasgenoemde. Die geografiese skeiding van die noordelike en sentrale klades stem grootliks ooreen met patrone in ander rotsbewonende vertebraat spesies, wat op ’n gedeelde biogeografiese verlede in Suid-Afrika dui. Elephantulus rupestris (westelike klipklaasneus) en Macroscelides proboscideus (ronde-oor klaasneus) is twee taksa met verspreidings wat grootliks oorvleuel in die semi-woestyn streke van Suid-Afrika en Namibië. Mitochondriale DNS volgorde-bepaling dui op ’n gestruktueerde genetiese profiel binne E. rupestris, geassosieer met ’n habitat van rotskoppies, in vergelyking met ’n isolasie-deur-afstand patroon wat M. proboscideus, wat op gruisvlaktes aangetref word, karakteriseer. Chromosoom verandering, afgesien van heterochromatiese verskille, is beperk tot ’n strukturele verandering van ‘n diploïede getal van 26 (E. edwardii; E. rupestris; Elephantulus sp. nov.; E. intufi; E. brachyrhynchus en M. proboscideus) tot 2n=28 in beide Petrodromus tetradactylus asook E. rozeti en 2n=30 in E. myurus. Kruis-spesies chromosoom fluoressent hibridisasie (“zoo-FISH”) van die vloei-sorteerde merkers toegewys tot die vyf kleiner grootte outosome (8-12) asook die X chromosoom van E. edwardii tot metafase chromosome van Elephantulus sp. nov., E. intufi, E. myurus, P. tetradactylus en M. proboscideus het geen bewyse getoon van sintenie-verbreking nie en versterk G-bandbepaling waarnemings wat die konserwatiewe kariotipes in hierdie spesies ondersteun. ‘n Volledige evolusionêre filogenie, verteenwoordigend van alle erkende klaasneusspesies, word vir die eerste keer voorgestel. As sulks is ’n multigeen supermatriks wat gebaseer is op 3905 bp van drie mitochondriale (12S rRNA, valien tRNA, 16S rRNA) en twee nukluêre segmente (Von Willebrand faktor [vWF] en ekson 1 van die interfotoreseptor-retinoïed-bindende proteïen [IRBP]) ingesluit. As toevoeging, is nuwe sitogenetiese data, voorheen beskryfde morfologiese, anatomiese en dentale karakters sowel as data van allosieme-analises en penis morfologie ge-evalueer en nie-molekulêre ondersteuning aangedui op die molekulêre topologie. Die molekulêre bevindinge ondersteun nie ’n monofiletiese oorsprong vir Elephantulus nie en stel voor dat beide die monotipiese Petrodromus en Macroscelides ingesluit moet word in die genus Elephantulus. Molekulêre datering stel voor dat ’n dor-aangepasde Macroscelidinae lyn versprei het vanaf oos Afrika ~11.5 miljoen jaar gelede deur die “droeë Afrika korridor” tot in suidwestelike Afrika. Verdere spesiasie gebeurtenisse binne die Macroscelidinae kan nouliks geassosieer word met drie groot periodes van verdorring in Afrika.