Submitted by Erika Demachki (erikademachki@gmail.com) on 2017-05-05T19:00:03Z No. of bitstreams: 2 Dissertação - Winnie Castro Amorim e Albuquerque - 2017.pdf: 2279498 bytes, checksum: 160e4a276e405c6393dd5bb7742b106b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-05-10T13:37:01Z (GMT) No. of bitstreams: 2 Dissertação - Winnie Castro Amorim e Albuquerque - 2017.pdf: 2279498 bytes, checksum: 160e4a276e405c6393dd5bb7742b106b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-05-10T13:37:01Z (GMT). No. of bitstreams: 2 Dissertação - Winnie Castro Amorim e Albuquerque - 2017.pdf: 2279498 bytes, checksum: 160e4a276e405c6393dd5bb7742b106b (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-03-31 / Outro / Bovine papillomavirus is the etiological agent of bovine papillomatosis, a disease that triggers warts throughout the skin, udder, roofs, genitalia and in more severe cases can develop extensive papillomas, cause neoplasia in the digestive tract and bladder, weaken the animal's health and cause losses in the Productivity and losses for livestock. The present study aims to detect and typify bovine Papillomavirus present in bovine tissue and blood samples with papillomatosis, to sequence the isolated viral types, to analyze the nucleotide sequences and the phylogeny of the detected viral types. As a result, amplification was obtained in five tissue samples (papilloma) from different bovines, not being successful in the amplification of blood samples. PCR reactions revealed the presence of BPV-1 in 60%, BPV-5 in 40%, BPV-9, BPV-10, BPV-13 and BPV-14 in 20% and BPV-12 in 40% of the analyzed samples. The presence of coinfection was verified in 60% of the lesions analyzed, with up to four viral types infecting the same sample. Alignments of viral type sequences 1, 5 and 14 were validated with identity ranging from 74% to 95%. The phylogenetic diagram showed a genetic approximation between viral types 1 and 14, both belonging to the genus Deltapapillomavirus, and distancing between nucleotide sequences of viral types 5, 9 and 14. Papillomaviruses of types 5 and 9 belong to different genera, Epsilonpapillomavirus and Xipapillomavirus, Respectively, the phylogenetic distance between these viral types, verified in the diagram, is justified. / Papilomavírus bovino é o agente etiológico da papilomatose bovina, doença que desencadeia verrugas por toda pele, úbere, tetos, genitália e em casos mais graves pode desenvolver papilomas extensivos, causar neoplasia no trato digestivo e bexiga, debilitar a saúde do animal e provocar perdas na produtividade e prejuízos para a pecuária. O presente estudo possui como objetivo detectar e tipificar Papilomavírus bovino presentes em amostras de tecido e sangue de bovinos com papilomatose, sequenciar os tipos virais isolados, analisar as sequências nucleotídicas e a filogenia dos tipos virais detectados. Como resultado, obteve-se amplificação em cinco amostras de tecido (papiloma) de diferentes bovinos, não obtendo sucesso na amplificação das amostras de sangue. As reações de PCR revelam a presença do BPV-1 em 60%, BPV-5 em 40%, BPV-9, BPV-10, BPV-13 e BPV-14 em 20% e BPV-12 em 40% das amostras analisadas. A presença de coinfecção foi verificada em 60% das lesões analisadas, com até quatro tipos virais infectando a mesma amostra. Os alinhamentos das sequencias do tipo viral 1, 5 e 14 foram validados com identidade variando de 74% a 95%. O diagrama filogenético demonstrou aproximação genética entre os tipos virais 1 e 14, ambos pertencentes ao gênero Deltapapillomavirus, e distanciamento entre as sequências nucleotídicas dos tipos virais 5, 9 e 14. Os papilomavírus dos tipos 5 e 9 pertencem a gêneros diferentes, Epsilonpapillomavirus e Xipapillomavirus, respectivamente, justifica-se dessa forma, a distância filogenética entre esses tipos virais, verificada no diagrama.