Thesis (Ph. D. (Plant Production)) -- University of Limpopo,2016 / Refer to document / University of Limpopo, The Technology Innovation Agency (TIA), The Land Bank Chair of Agriculture ─ University of Limpopo, The Flemish Interuniversity Council (VLIR) and, The Agricultural Research Council - University Collaboration Centre

MSCAGR (Agricultural Economics) / Department of Agricultural Economics and Agribusiness / study aimed to determine factors that influenced the price of tomatoes sold by hawkers on the roadside market. The study also aimed at determining whether any socio-economic characteristics of hawkers who sold tomatoes had significant impact on the price paid my consumers. The study was conducted in the Thulamela local municipality under Vhembe District of Limpopo, South Africa. A stratified random sampling technique was used to select participants for this study. A sample size of 198 hawkers were drawn randomly. Structured questionnaires were administered through face-to-face interview. Descriptive statistics, correlation matrix, multiple linear hedonic regression and stepwise linear regression were used to analyse collected data. With regards socio-economic characteristics, only age was found to have significant impact on the market price received by hawkers. Smooth appearance, rough appearance, round shape, oval shape, and red and yellow colour tomatoes had a significant impact on the average market price. It is therefore recommended that attribute such appearances, colour, shapes and sizes of tomatoes should be considered when selling tomatoes at both retail and farm level. This will result in more purchase of tomatoes that will eventually contribute to increase in household income and reduction in poverty.

The tomato is one of the most important vegetable crops grown in the South African community. Most hydroponic tomato growers in South Africa are using sawdust as a growing medium due to its availability and affordability. However, there is little or no information on how particle sizes of sawdust influence tomato yield and quality. The aim of the study was to determine the effect of the particle size of sawdust on plant growth, yield and quality of tomato. Six treatments of different particle sizes of sawdust i.e. fine (F), medium (M), coarse (C) and 50:50 ratio of F: M, C: M, and C: F extracted from pine tree were used for the experiment. Treatments were arranged in a randomized complete block design with four replicates. The size of the sawdust particles did not have a significant effect on plant height, stem diameter, leaf length and width, shelf-life, marketable yield, total yield and unmarketable yield. A tendency to increase marketable and total yield was observed when tomato plants were grown at a 50:50 C: F ratio. Fruit and leaf mineral content were not affected by sawdust particle size. After completion of the experiment, air- filled porosity was significantly high on particle size C, M, and C: M while the water holding capacity was significantly high on F followed by M. The study showed that the suitable growth medium for production of tomatoes under the hydroponics system used was the CF particle substrate. Generally, the experimental crop performed better under the CF particle substrate in terms of growth parameters, and fruit quality, thus leading to the conclusion that the CF growth medium is ideal for hydroponically grown tomato under a non-environmentally controlled polytunne / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

Submitted in fulfilment of the requirements for the degree of Master of Applied Science in Food Science and Technology, Durban University of Technology, 2015. / Listeria monocytogenes have been implicated as a public health concern worldwide. The study explored the survival of non-adapted, heat adapted and chlorine adapted L. monocytogenes on tomatoes; as well as the survival of non-adapted, heat adapted and chlorine adapted biofilms after exposure to sodium dodecyl sulphate (SDS), levulinc acid, sodium hypochlorite solution. Contact time of 1, 3 and 5 minutes was used. The survival of L. monocytogenes was monitored at 0, 24, 48 and 72 hours. The sanitizers were used individually or combined as follows; 1% sodium dodecyl sulphate individually; 0.5% levulinic acid individually; 200 ppm sodium hypochlorite solution individually and 0.5% levulinic acid/0.05% sodium dodecyl sulphate in combination (mixture). The samples were kept at 4 °C throughout the period of assessment. The effect of these sanitizers on pH, total soluble solids (TSS) and titratable acidity (TA) was also determined. Furthermore, the attachment of L. monocytogenes on tomatoes was investigated using a scanning electron microscope. Highest log reduction of non-adapted L. monocytogenes were observed on tomatoes treated with 1% SDS and least log reduction was achieved when tomatoes were treated with sodium hypochlorite solution. Though the log reduction achieved by 0.5% levulinic acid was higher that sodium hypochlorite solution, it was lower than log reduction achieved when 0.05% SDS / 0.5% levulinic acid mixture was used for all contact times. Using non-adapted L. monocytogenes, SDS was able to destroy all L. monocytogenes at 1, 3 and 5 minutes contact time. The trend was the same when heat adapted and chlorine adapted L. monocytogenes were used. There was no significant log reduction observed with biofilms. More favourable results were observed as contact time was increased from 1 to 5 minutes. Though there was a decrease in surviving bacteria from 1 to 3 minutes contact time, this decrease was not significant. The study investigated if exposure to sanitizer has an effect on pH, titratable acidity (TA) and total soluble solids (TSS) of the tomatoes. It was revealed that levulinic acid and mixture can have detrimental effect on pH, TA and TSS of tomatoes. The TA and TSS of samples treated with levulinic acid and mixture varied significantly (P ≤ 0.05) compared to the control sample. Although the TA and TSS of samples treated with SDS and sodium hypochlorite solution were different from the control, the differences were not significant. As much as sanitizers have the potential to reduce the bacterial population in fresh produce they may not completely destroy pathogens. Chlorine based sanitizers such as sodium hypochlorite though frequently used in the fresh produce industry, are not the best sanitizer to be used against food borne pathogens. Other sanitizers such as SDS used alone or in combination with another sanitizer can achieve better results than the widely used sodium hypochlorite solution as observed in this study. Stress adapted pathogens become less responsive to sanitizers during subsequent treatments. Through this research, it was established that biofilms are resistant to sanitizers. Though application of sanitizers in fresh produce is cheaper and simpler to apply, there is need to monitor varying concentrations of sanitizers, contact time and minimise contact with sub-surfaces as this could lead to sensory quality losses.

The tomato is one of the most important vegetable crops grown in the South African community. Most hydroponic tomato growers in South Africa are using sawdust as a growing medium due to its availability and affordability. However, there is little or no information on how particle sizes of sawdust influence tomato yield and quality. The aim of the study was to determine the effect of the particle size of sawdust on plant growth, yield and quality of tomato. Six treatments of different particle sizes of sawdust i.e. fine (F), medium (M), coarse (C) and 50:50 ratio of F: M, C: M, and C: F extracted from pine tree were used for the experiment. Treatments were arranged in a randomized complete block design with four replicates. The size of the sawdust particles did not have a significant effect on plant height, stem diameter, leaf length and width, shelf-life, marketable yield, total yield and unmarketable yield. A tendency to increase marketable and total yield was observed when tomato plants were grown at a 50:50 C: F ratio. Fruit and leaf mineral content were not affected by sawdust particle size. After completion of the experiment, air- filled porosity was significantly high on particle size C, M, and C: M while the water holding capacity was significantly high on F followed by M. The study showed that the suitable growth medium for production of tomatoes under the hydroponics system used was the CF particle substrate. Generally, the experimental crop performed better under the CF particle substrate in terms of growth parameters, and fruit quality, thus leading to the conclusion that the CF growth medium is ideal for hydroponically grown tomato under a non-environmentally controlled polytunne / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)

Effective microorganisms (EM), a commercial concoction of microbes that includes yeasts, fungi, bacteria and actinomycetes, have been found to be effective in enhancing crop growth by a number of scholars. It is registered in South Africa, but it had not been thoroughly investigated. The present study investigated the effects of EM on growth, yield and quality of tomato (Lycopersicon esculentum Mill), butternut (Curcurbita moschata) and Swiss chard (Beta vulgaris), along with selected soil properties. In field-grown tomato it was observed that the application of EM caused a significant increase in the number of fruits at seven weeks after transplanting. However, plants treated with EM alone, or EM in combination with other amendments, subsequently produced lower yields owing to an outbreak of early and late blights which affected them the most severely. Combined applications of EM with organic amendments improved plant N content and increased soil N content above initial levels. The application of compost resulted in soil N and P concentrations higher than those of the control presumably due to nutrients being slowly released from the compost material. In a follow up greenhouse trial EM application had a negative effect on tomato leaf dry matter yield, number of leaves, number of trusses, fruit yield and number of fruits. The negative effects of EM were ascribed to N immobilization by the EM that could have resulted in reduced N availability to plants. The lower number of fruits associated with EM application resulted in improved average fruit weight of tomatoes grown in the greenhouse, possibly as a result of more assimilates being partitioned to the few fruits EM application also had a negative effect on field grown butternut as reflected by lower total yield, lower marketable yield and lower first grade yield. The results were attributed to immobilization of N induced by application of EM, and to the inability of EM to control pumpkin fly that attacked very young fruit, resulting in their failure to develop or resulting in the down grading of mature fruits. The application of EM alone had a positive but non significant effect on the yields of both the first and second harvests of Swiss chard. However, when applied with compost or goat manure, a non significant negative effect on yield was observed. When applied with inorganic fertilizer, EM had no effect on yield but tended to increase the uptake of nitrogen by Swiss chard. Though goat manure had a narrower C: N ratio than compost, it did not result in greater EM effectiveness as had been hoped. However, goat manure had a more positive effect on soil properties than compost. It increased the N, P, and K contents of the soil and resulted in a narrower C: N ratio of the soil compared to compost. Generally, the results of the four trials conducted with three different crops indicated that EM had inconsistent effects on crop performance.