Thesis (doctoral)--Karolinska Institutet, Stockholm, 1987. / Added t.p. with thesis statement inserted. Includes bibliographical references.

Thesis (doctoral)--Karolinska Institutet, Stockholm, 1987. / Added t.p. with thesis statement inserted. Includes bibliographical references.

Indiana University-Purdue University Indianapolis (IUPUI) / It has been debated as to whether teeth may actually be intruded in their alveoli. This study was designed to measure the intrusive movement and histologic response of specific teeth to a known intrusive force. Four Macaque speciosa monkeys were used. The animals corresponded in age to a 12-14 year old human being. Intrusive forces of either 10, 50, 100, or 300 grams were placed on the maxillary first premolars; each force value was held constant during the duration of the experiment, which was 60 days. Maxillary first molars were used as anchorage. Cephalometric headplates proved that all force values were capable of giving intrusion. The 50 gram forces, however, gave the most efficient intrusion. Histologic sections were made on all experimental teeth. 300 and 100 gram forces gave excessive root resorption but the 50 and 10 gram force values elicited only slight root resorption. Root resorption seemed to be directly related to excessive force. The amount of intrusion was not related to a loss of root structure. Bone resorption appeared to allow the intrusion of teeth with minimal tissue damage if proper force values were used.

Thesis (doctoral)--Munich, 1933. / Includes bibliographical references (p. [18]).

Thesis (doctoral)--Munich, 1933. / Includes bibliographical references (p. [18]).

Orientador: Celso Koogi Sonoda / Banca: Fábio Yoshio Tanaka / Banca: Idelmo Rangel Garcia Júnior / Banca: Sonia Regina Panzarini Barioni / Banca: Mirian Marubayashi Hidalgo / Resumo: O Euro-Collins é um meio de conservação desenvolvido para manutenção de órgãos para transplante. Suas características despertaram o interesse na sua utilização como meio de conservação de dentes avulsionados. Nesse contexto, o objetivo deste trabalho foi realizar a análise histológica e morfométrica do processo de reparo do reimplante de dentes de cães mantidos previamente em solução de Euro-Collins ou leite bovino. Foram utilizadas 80 raízes de quatro cães adultos jovens, divididos em 4 grupos de 20 raízes. Inicialmente foi realizado o tratamento endodôntico de todos os dentes. Após duas semanas, realizaram-se as exodontias e os dentes extraídos receberam os seguintes tratamentos: no grupo I, foram reimplantados imediatamente após a exodontia; no grupo II, foram mantidos em meio seco sobre bancada, por 2 horas e reimplantados; no grupo III, foram mantidos em um frasco contendo 50ml de leite bovino integral por 8 horas e reimplantados; no grupo IV, os dentes foram mantidos em um frasco contendo 50ml de solução de Euro-Collins® por 8 horas e reimplantados. Nos grupos III e IV, as soluções foram mantidas refrigeradas a 4° C. Os resultados demonstraram que todos os grupos foram comprometidos pelas reabsorções radiculares sendo que a perda de estrutura radicular foi maior no grupo II com diferença estatisticamente significante (p<0,01). Neste grupo houve predomínio da reabsorção inflamatória. A manutenção do dente em leite se mostrou menos eficaz do que o reimplante imediato ou do uso da solução de Euro-Collins® (p<0,01). Quanto ao nível de reabsorção e a reorganização do ligamento periodontal, os dentes mantidos em Euro-Collins® apresentaram resultados semelhantes aos dentes imediatamente reimplantados. Este achado permite considerá-lo adequado para uso como meio de conservação por até 8 horas. / Abstract: Euro-Collins is a storage media developed for maintenance of the organ for transplantation. The solution characteristics encouraged the interestingness to its use as a storage media for avulsed teeth prior to replantation. The purpose of this study was to perform the histologic and morphometric analysis of the healing process of delayed replanted canine tooth maintained in Euro-Collins® solution and bovine milk. Eighty roots of four mongrel adult dogs were used in this study, divided in 4 groups of 20 roots. Initially, endodontic treatment was performed in all involved teeth. After two weeks, the teeth was extracted and then received the following treatments: group I, the teeth were immediately replanted after extraction; group II, the teeth were bench-dried for 2 hours before replantation; group III, the teeth were maintained immersed in 50ml of bovine milk during 8 hours before replantation; and group IV, the teeth were maintained immersed in 50ml of Euro-Collins solution during 8 hours before replantation. In the groups III and IV the recipients containing the solution and teeth were maintained under refrigeration at 4oC. The animals were euthanized at 90 days postoperative. The results revealed radicular resorption in all groups, whereas the Group II exhibited the greater lost of dental structure, statistically different (p<0,01). Inflammatory resorption was predominant in this group. The milk as storage media showed poor results comparing to the immediately replantation and maintenance into Euro-Collins® solution approach (p<0,01). In the group IV, in which the teeth was maintained into Euro-Collins as storage media, the radicular resorption level and reorganization of the periodontal ligament were similar to the immediately replanted teeth. On the basis of these findings, it can be considered that the Euro-Collins® is an adequate solution that can be used as a storage media, until 8 hours, to maintenance of the avulsioned teeth. / Doutor

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Background and Objectives: The proven efficacy of bisphosphonates to inhibit the osteoclastic bone resorption has led to their use in the management of periodontal diseases. This dissertation, comprised by 2 manuscripts, aimed: (1) to histologically analyze the effects of systemic administration of Tiludronate (TIL) on ligature-induced periodontitis in rats; (2) to histologically analyze the effects of systemic administration of TIL as an adjunctive therapy to mechanical periodontal treatment on ligature-induced periodontitis in rats. Methods: In study 1, 32 adult male rats were divided into four groups (n=8): C, PD, PD-TIL5, PD-TIL15 (CâControl group, PDâPeriodontitis groups). On PD groups, a ligature was placed in the cervical area of the right mandibular 1st molar of each rat. After 15 days, TIL solutions (TildrenÂ, Ceva SaÃde Animal Ltda., PaulÃnia, SP, Brazil) at dosages of 5 mg/kg body weight (group PD-TIL5) or 15 mg/kg body weight (group PD-TIL15) were subcutaneously administered 5 times a week for 3 weeks. In study 2, 40 adult male rats were divided into five groups (n=8): C, PD, PDT, PDT-TIL 5, PDT-TIL 15. On PD groups, ligatures were placed as described. After 15 days, ligatures of the rats from groups PDT, PDT-TIL5 and PDT-TIL15 were removed and scaling and root planing were performed. TIL solutions at dosages of 5 mg/kg body weight (group PDT-TIL5) or 15 mg/kg body weight (group PDT-TIL15) were subcutaneously administered 5 times a week for 3 weeks. All animals were euthanized at the 36th day. Histometric and histologic analyses were performed. Data were statistically analyzed (ANOVA, Tukey, p<0.05). Results: In study 1, alveolar bone loss was significantly reduced in group PD-TIL5 (1.12 mmÂ0.24), when compared with groups PD (1.70 mmÂ0.32) and PD-TIL15 (1.47 mmÂ0.21). The animals from all PD groups presented more periodontal attachment loss than the ones from group C (0.12 mmÂ0.09). There were no differences in periodontal attachment loss among PD groups (PD: 0.53 mmÂ0.19; PD-TIL5: 0.37 mmÂ0.09; PD-TIL15: 0.52 mmÂ0.13). In study 2, there were no differences in alveolar bone losses among groups PDT (1.27 mmÂ0.15), PDT-TIL 5 (1.18 mmÂ0.10) and PDT-TIL 15 (1.26 mmÂ0.40). The alveolar bone losses found in these groups were slighter than the alveolar bone loss observed in group PD and did not statistically differ from the alveolar bone loss found in group C. Animals from all groups with periodontitis induction (group PD: 0.59 mmÂ0.16; group PDT: 0.39 mmÂ0.07; group PDT-TIL 5: 0.42 mmÂ0.05; group PDT-TIL 15: 0.48 mm  0.09) presented periodontal attachment losses statistically greater than the animals from group C (0.12 mmÂ0.09). Groups PDT and PDT-TIL 5 presented less periodontal attachment loss than group PD. Conclusions: Within the limits of this study, it can be concluded that (i) systemically-administered TIL solution reduced alveolar bone loss in established periodontitis in rats, (ii) dosage of TIL may influence its anti-inflammatory and anti-resorptive properties and (iii) systemically-administered TIL did not result in additional benefits to periodontal mechanical therapy in rats with experimental periodontitis. / IntroduÃÃo e Objetivos: A eficÃcia comprovada dos bisfosfonatos em inibir a reabsorÃÃo Ãssea osteoclÃstica levou à utilizaÃÃo dos mesmos no tratamento da periodontite. Esta dissertaÃÃo, composta por 2 artigos, teve como objetivos: (1) avaliar, histologicamente, os efeitos da administraÃÃo sistÃmica do bisfosfonato Tiludronato (TIL) na periodontite induzida por ligadura em ratos; (2) avaliar, histologicamente, os efeitos da administraÃÃo sistÃmica do TIL como terapia adjuvante ao tratamento periodontal mecÃnico na periodontite induzida por ligadura em ratos. MÃtodos: No estudo 1, 32 ratos adultos machos foram divididos em 4 grupos (n=8): C, DP, DP-TIL5 e DP-TIL15 (C-grupo Controle, DP-grupos Periodontite). Nos grupos DP, ligaduras foram colocadas na Ãrea cervical dos 1os molares inferiores direitos de cada um dos ratos no 1 dia. ApÃs 15 dias, soluÃÃes de TIL (TildrenÂ, Ceva SaÃde Animal Ltda., PaulÃnia/SP, Brasil) nas dosagens de 5 mg/kg de peso corporal (grupo DP-TIL5) e 15 mg/kg de peso corporal (grupo DP-TIL15) foram administradas, 5 vezes por semana, durante 3 semanas. No estudo 2, 40 ratos adultos machos foram divididos em 5 grupos (n=8): C, DP, DPT, DPT-TIL5 e DPT-TIL15. Nos grupos DP, foram colocadas ligaduras, conforme descriÃÃo anterior. ApÃs 15 dias, as ligaduras dos ratos dos grupos DPT, DPT-TIL5 e DPT-TIL15 foram removidas, e foram realizados raspagem e alisamento radicular. SoluÃÃes de TIL nas dosagens de 5 mg/kg de peso corporal (DPT-TIL5) e 15 mg/kg de peso corporal (DPT-TIL15) foram administradas, 5 vezes por semana, durante 3 semanas. Os animais foram submetidos à eutanÃsia no 36 dia. Foram realizadas anÃlises histolÃgica qualitativa e histomÃtrica. Os dados obtidos foram analisados estatisticamente (ANOVA, Tukey, p< 0,05). Resultados: No estudo 1, a perda Ãssea alveolar foi significativamente reduzida no grupo DP-TIL5 (1,12 mmÂ0,24), quando comparada à dos grupos DP (1,70 mmÂ0,32) e DP-TIL15 (1,47 mmÂ0,21). Os animais dos grupos DP apresentaram maior perda de inserÃÃo quando comparados aos do grupo C (0,12 mmÂ0,09). NÃo houve diferenÃas na perda de inserÃÃo entre os grupos DP (DP: 0,53 mmÂ0,19; DP-TIL5: 0,37 mmÂ0,09; DP-TIL15: 0,52 mmÂ0,13). No estudo 2, nÃo houve diferenÃas na perda Ãssea alveolar entre os grupos DPT (1,27 mmÂ0,15), DPT-TIL 5 (1,18 mmÂ0,10) e DPT-TIL 15 (1,26 mmÂ0,40). A perda Ãssea alveolar observada nesses grupos foi menor que a do grupo DP e nÃo diferiu estatisticamente da perda Ãssea alveolar encontrada no grupo C. Todos os animais dos grupos com ligadura (grupo DP: 0,59 mmÂ0,16; grupo DPT: 0,39 mmÂ0,07; grupo DPT-TIL 5: 0,42 mmÂ0,05; grupo DPT-TIL 15: 0,48 mm  0,09) apresentaram perdas de inserÃÃo estatisticamente maiores que os animais do grupo C (0,12 mmÂ0,09). Os grupos DPT e DPT-TIL 5 apresentaram menor perda de inserÃÃo que o grupo DP. ConclusÃes: Dentro dos limites deste estudo, pode ser concluÃdo que (i) a administraÃÃo sistÃmica de TIL reduziu a perda Ãssea alveolar na periodontite estabelecida em ratos; (ii) a dosagem do TIL pode influenciar suas propriedades antirreabsortivas e anti-inflamatÃrias; (iii) a administraÃÃo sistÃmica de TIL nÃo proporcionou benefÃcios adicionais à terapia periodontal mecÃnica em ratos com periodontite experimental.

Information on dental resorption first appeared in the literature in an 1829 textbook by Thomas Bell. What first was mistaken as bone tumor and known as “absorption,” continues to be one of the most challenging and mysterious phenomena dental clinicians have had to address. Over the past years, there has been a dramatic increase in reports of resorption in the literature, which have aided in the understanding of the condition and its treatments. Resorption is identified by an increase in osteoclastic cell activity that causes a loss of hard dental tissues. As a physiological process, root resorption helps with the eruption and exfoliation of primary teeth. In adults, resorption is of pathological nature. Resorption can happen both internally and externally. External resorption is a much more common occurrence in dentistry than its counterpart, internal resorption, and involves the external aspect of the tooth. External resorption has been subclassified into external inflammatory resorption, external replacement resorption, external cervical resorption, external surface resorption, and transient apical breakdown. Internal resorption is more unusual and more challenging to diagnose and affects the tooth’s pulp chamber and/or its root canal. Internal resorption has been sub-classified as internal inflammatory resorption and internal replacement resorption. Descriptions of the various forms of resorption are numerous in the literature and have become available due to case studies, clinical presentations and treatment options. From trauma, to prolonged orthodontic treatment, to viruses, to genetic and idiopathic factors, there is a variety of possible etiologic causes of both kinds of resorptions that are central to the understanding and treatment of this condition. Despite the many advances in the field, however, there are still gaps in the processes leading to resorption lesions that remain to be elucidated. If OPG and RANKL are the major culprits in initiating resorption, being able to arrest these molecules or transcription factors, such as c-fos and NFkB or identifying genetic propensities for resorption with a BRCA-like test seem to be research goals which may translate into the prevention of resorption as well as identifying how a physiological process essential to survival transforms into a pathological condition. Additionally and equally important, when resorption is suspected, there is the need for a thorough examination of the oral cavity and a proper understanding of the underlying pathogenesis for its clinical management. Depending on the extent of the lesion, resorption can be arrested by a variety of endodontic treatments that often include root canals or more complex surgical procedures, such as muco-periosteal flaps that allow the access to the resorptive lesion and the excavation of the granulomatous tissue in the tooth. Moreover, composite, sodium hypochlorite or calcium hydroxide are also employed as treatment/ preventive options as well as breaks in orthodontic procedures to eliminate pressure forces that contribute to and/or cause resorption, pulpectomy, careful monitoring, among others. Whether external or internal resorption, the condition can be treated with high rate of success if caught early. Without intervention, resorption leads to tooth loss.

Made available in DSpace on 2014-06-11T19:31:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-12-19Bitstream added on 2014-06-13T19:20:08Z : No. of bitstreams: 1 sottovia_ad_dr_araca.pdf: 20246955 bytes, checksum: 1945c663b8d25d4878f64606fb881206 (MD5) / O Euro-Collins é um meio de conservação desenvolvido para manutenção de órgãos para transplante. Suas características despertaram o interesse na sua utilização como meio de conservação de dentes avulsionados. Nesse contexto, o objetivo deste trabalho foi realizar a análise histológica e morfométrica do processo de reparo do reimplante de dentes de cães mantidos previamente em solução de Euro-Collins ou leite bovino. Foram utilizadas 80 raízes de quatro cães adultos jovens, divididos em 4 grupos de 20 raízes. Inicialmente foi realizado o tratamento endodôntico de todos os dentes. Após duas semanas, realizaram-se as exodontias e os dentes extraídos receberam os seguintes tratamentos: no grupo I, foram reimplantados imediatamente após a exodontia; no grupo II, foram mantidos em meio seco sobre bancada, por 2 horas e reimplantados; no grupo III, foram mantidos em um frasco contendo 50ml de leite bovino integral por 8 horas e reimplantados; no grupo IV, os dentes foram mantidos em um frasco contendo 50ml de solução de Euro-Collins® por 8 horas e reimplantados. Nos grupos III e IV, as soluções foram mantidas refrigeradas a 4° C. Os resultados demonstraram que todos os grupos foram comprometidos pelas reabsorções radiculares sendo que a perda de estrutura radicular foi maior no grupo II com diferença estatisticamente significante (p<0,01). Neste grupo houve predomínio da reabsorção inflamatória. A manutenção do dente em leite se mostrou menos eficaz do que o reimplante imediato ou do uso da solução de Euro-Collins® (p<0,01). Quanto ao nível de reabsorção e a reorganização do ligamento periodontal, os dentes mantidos em Euro-Collins® apresentaram resultados semelhantes aos dentes imediatamente reimplantados. Este achado permite considerá-lo adequado para uso como meio de conservação por até 8 horas. / Euro-Collins is a storage media developed for maintenance of the organ for transplantation. The solution characteristics encouraged the interestingness to its use as a storage media for avulsed teeth prior to replantation. The purpose of this study was to perform the histologic and morphometric analysis of the healing process of delayed replanted canine tooth maintained in Euro-Collins® solution and bovine milk. Eighty roots of four mongrel adult dogs were used in this study, divided in 4 groups of 20 roots. Initially, endodontic treatment was performed in all involved teeth. After two weeks, the teeth was extracted and then received the following treatments: group I, the teeth were immediately replanted after extraction; group II, the teeth were bench-dried for 2 hours before replantation; group III, the teeth were maintained immersed in 50ml of bovine milk during 8 hours before replantation; and group IV, the teeth were maintained immersed in 50ml of Euro-Collins solution during 8 hours before replantation. In the groups III and IV the recipients containing the solution and teeth were maintained under refrigeration at 4oC. The animals were euthanized at 90 days postoperative. The results revealed radicular resorption in all groups, whereas the Group II exhibited the greater lost of dental structure, statistically different (p<0,01). Inflammatory resorption was predominant in this group. The milk as storage media showed poor results comparing to the immediately replantation and maintenance into Euro-Collins® solution approach (p<0,01). In the group IV, in which the teeth was maintained into Euro-Collins as storage media, the radicular resorption level and reorganization of the periodontal ligament were similar to the immediately replanted teeth. On the basis of these findings, it can be considered that the Euro-Collins® is an adequate solution that can be used as a storage media, until 8 hours, to maintenance of the avulsioned teeth.

Objetivou-se realizar um estudo retrospectivo sobre a frequência de cães acometidos pela reabsorção dentária (RD) atendidos no Laboratório de Odontologia Comparada da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, no período de 2006 a 2017, correlacionando com características como raça, idade, sexo, doenças orais concomitantes, hábito alimentar, comportamento mastigatório, histórico dental e sinais clínicos relacionados a dor, bem como, relatar estatisticamente os grupos dentários frequentemente acometidos, porções dentárias mais afetadas, aspectos radiográficos e extensão da reabsorção de acordo com a classificação determinada pelo American Veterinary Dental College (AVDC). Os dados avaliados procederam de 2.364 prontuários contendo fichas de consulta e de tratamento, além da análise de 1.122 registros radiográficos. Constatou-se que a prevalência geral da RD em cães foi de 8,7% e o ano mais frequente foi 2016 com 21,2%. Os pré-molares mandibulares e os maxilares foram significativamente mais afetados (37,3% e 38,1% respectivamente) e a porção radicular apresentou-se mais acometida (60,5%). As lesões do tipo 1 foram significativamente mais frequentes (53,2%) que as lesões do tipo 2 (27,9%) e tipo 3 (8,1%). Não houve lesões classificadas no estágio 1. Os estágios da reabsorção dentária mais frequentes foram o RD3 com 34,3% e o RD2 com 29,5%. Os estágios 4a, 4b e 4c representaram respectivamente 3,3%, 1,8% e 12,8%. No estágio 5, detectou-se apenas fragmentos de raiz em estágio avançado da reabsorção (2,3%). A reabsorção dentária foi associada estatisticamente com os cães de idades avançadas (10 e 13 anos com 14,6% cada). Não houve diferença significativa entre a frequência de machos e fêmeas, mas, a frequência de cães castrados (42,3%) foi significativamente menor do que a de não castrados (57,7%). A doença periodontal mostrou-se significativamente associada à reabsorção dentária (84,0%), seguida das fraturas dentárias (20,9%). Os cães acometidos pela RD alimentavam-se somente de ração (90,5%), premium ou super premium (86,6%). A maioria dos cães apresentaram-se assintomáticos no pré-operatório (82,0%) e sem alteração do comportamento no pós-operatório (39,3%). O tratamento de escolha para 66,5% dos casos foi a extração dentária. Concluiu-se que: a reabsorção inflamatória externa ou reabsorção do tipo 1, mostrou-se mais frequente quando comparada com a reabsorção de substituição externa (tipo 2). Sugere-se que boa parte da reabsorção dentária dos cães avaliados nesse estudo, esteja diretamente relacionada com processos inflamatórios desencadeados pela doença periodontal, uma vez que, evidenciou-se correlação estatística significativa entre a reabsorção dentária e a doença periodontal. / The objective of this study was to conduct a retrospective study on the frequency of dogs affected by tooth resorption attended at the Laboratory of Comparative Dentistry of the Faculty of Veterinary Medicine and Animal Science of the University of São Paulo from 2016 to 2017, correlating with characteristics such as race, age, sex, concomitant oral diseases, eating habits, masticatory behavior, dental history and clinical signs related to pain, as well as, statistically reporting the frequently affected dental groups, most affected dental portions, radiographic aspects and extent of resorption according to the classification determined by the American Veterinary Dental College (AVDC). The data evaluated came from 2,364 medical records containing records of consultation and treatment, in addition to the analysis of 1,122 radiographic records. It was found that the overall prevalence of tooth resorption in dogs was 8.7% and the most frequent year was 2016 with 21.2%. The mandibular and maxillary premolars were significantly more affected (37.3% and 38.1%, respectively) as well as the root portion (60.5%). Type 1 lesions were significantly more frequent (53.2%) than type 2 (27.9%) and type 3 (8.1%) lesions. There were no lesions classified in stage 1. The most frequent stages of tooth resorption were E3 with 34.3% and E2 with 29.5%. Stages 4a, 4b and 4c represented 3.3%, 1.8% and 12.8%, respectively. In stage 5, only root fragments were detected at an advanced stage of reabsorption (2.3%). Dental resorption was statistically associated with dogs at advanced ages (10 and 13 years old with 14.6% each). There was no significant difference between the frequency of males and females, but the frequency of castrated dogs (42.3%) was significantly lower than that of non-castrated dogs (57.7%). Periodontal disease was significantly associated with tooth resorption (84.0%), followed by dental fractures (20.9%). The dogs affected by tooth resorption were only fed commercial food (90.5%), premium or super premium (86.6%). Most of the dogs were asymptomatic in the preoperative period (82.0%) and no change in postoperative behavior (39.3%). The treatment of choice for 66.5% of the cases was dental extraction. It was concluded that: The external inflammatory resorption or type 1, was more frequent when compared to external replacement resorption (type 2). It is suggested that a good part of the dental resorption of the dogs evaluated in this study is directly related to inflammatory processes triggered by periodontal disease, since a significant statistical correlation between dental resorption and periodontal disease was evidenced.