The purpose of this study is to explore the relationship between the attitudes toward women of female managers, the quality of leader-member exchange between female managers and their female subordinates, and female managers¡¦queen bee behavior which female subordinates perceived. Moreover, this study examined seven related moderator variables within the relationship between the attitudes toward women of female managers and their queen bee behavior female subordinates perceived. We collected 140 matched questionnaires from female managers and one of their random female subordinates as a complete set in various industries, and 129 of 140 sets are valid. Our results showed that the attitudes toward women of female managers had a significant positive relationship with queen bee behavior female subordinates perceived, which means the more traditional attitudes toward women female managers hold, the higher degree queen bee behavior their female subordinates perceived. Besides, we found a significant negative relationship between the quality of leader-member exchange between female managers and their female subordinates, and queen bee behavior female subordinates perceived, which indicates the lower the exchange quality between female managers and their female subordinates, the higher degree queen bee behavior female subordinates perceived. And we also found that marital status of female managers and the female portion in their departments moderated the relationship between the attitudes toward women of female managers and queen bee behavior female subordinates perceived. Further practical implications and suggestions are also discussed in this study.