Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T14:00:08Z No. of bitstreams: 2 victor_hugo_duarte_costa.pdf: 749112 bytes, checksum: 673a3fb7e223fa45ab64a0cf8798bedf (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2016-01-05T18:02:07Z (GMT) No. of bitstreams: 2 victor_hugo_duarte_costa.pdf: 749112 bytes, checksum: 673a3fb7e223fa45ab64a0cf8798bedf (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2016-01-05T18:02:07Z (GMT). No. of bitstreams: 2 victor_hugo_duarte_costa.pdf: 749112 bytes, checksum: 673a3fb7e223fa45ab64a0cf8798bedf (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2015 / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Lagartas da esp?cie Helicoverpa armigera foram identificadas no Brasil atacando extensas ?reas de produ??o agr?cola. Algumas caracter?sticas fazem deste inseto uma das principais pragas a sistemas agr?colas do mundo, o que torna este ataque um perigo eminente ?s ?reas de produ??o do pa?s. Estudos com os insetos coletados nestas ?reas s?o essenciais para que seja desenvolvido um manejo adequado. Estudos de laborat?rio podem selecionar poss?veis pr?ticas de manejo a fim de combater surtos de pragas no campo. Uma das formas de manejar o ambiente de forma segura ? adotar o Manejo Integrado de Pragas, onde se destaca o controle biol?gico utilizando baculov?rus. A lagarta de H. armigera ? suscept?vel a estirpes de baculov?rus, por?m ? necess?rio verificar esta suscetibilidade. Sendo assim, os objetivos deste trabalho foram selecionar estirpes aut?ctones de baculov?rus t?xicas as lagartas desta esp?cie. Foram realizados testes gen?ticos e biol?gicos entre as estirpes aut?ctones, comparando-as com o produto comercial Gemstar?, de origem norte americana. A an?lise comparativa do sequenciamento gen?tico realizada para os genes LEF-8 e LEF-9 revelaram que os isolados locais est?o, estreitamente, relacionados com esp?cies de baculov?rus da Austr?lia e da ?ndia. Todos os isolados testados possibilitaram o controle de lagartas de terceiro instar de H. armigera. Por?m, analises biol?gicas da concentra??o letal m?dia (CL50) e do tempo letal m?dio (TL50) variaram entre os isolados testados. O isolado HearNPV-BR2 apresentou os melhores resultados de CL50 e TL50. Ademais, este ? o primeiro registro da ocorr?ncia da esp?cie HearNPV-BR2 no Brasil, e suas propriedades inseticidas assinalam que a mesma pode ser ?til para a fabrica??o de bioinseticidas para o controle de H. armigera no pa?s. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2015. / ABSTRACT Larvea of Helicoverpa armigera species were identified in Brazil attacking extensive agricultural production areas. Some features make this one of the main insect pests in agricultural systems in the world, which makes this attack an imminent danger to agricultural production in the country. Studies with insects collected in these areas are essential for developed proper management. Laboratory studies can select possible management practices to combat pest outbreaks in the field. One way to manage the secure environment is to adopt the Integrated Pest Management, highlighting the biological control using baculovirus. The H. armigera larvea is susceptible to strains of baculovirus but it is necessary check this susceptibility. Therefore the objectives of this work were to select indigenous strains of toxic baculovirus larvea of this species. Genetic and biological tests were carried out between indigenous strains, comparing them with the commercial product Gemstar?, origin from North American (USA). Comparative analysis of genetic sequencing performed for the LEF-8 and LEF-9 genes, revealed that indigenous strains are closely related to species of baculovirus of the Australia and India. All strains tested allowed control of the third instar of the H. armigera larvea. However, biological analysis of median lethal concentration (LC 50) and median lethal time (LT50) varied among the strains tested. The HearNPV-BR2 strain showed the best results of CL50 and TL50. Moreover, this is the first record of the occurrence of HearNPV-BR2 species in Brazil and its insecticidal properties indicate that BR2 may be useful for the manufacture of biological insecticides for H. armigera control in the country.