Thesis (doctoral)--Katholieke Universiteit van Nijmegen.

Inaug.-Diss. Rechtswiss. Bochum, 2007.

Blood sugar levels, and tumor size and incidence were determined for alloxanized Wistar rats bearing subcutaneous and intraperitoneal transplants of Novikoff hepatoma and Walker 256 carcinoma, and histological examination of the pancreas was undertaken to determine the extent of the damage to the beta-cells of the islets of Langerhans. Experiments were conducted in which animals were alloxanized 14 days, 7 days, 4 days, and 24 hours prior to, and 24 hours and 4 days after transplanting. The incidence and rate of growth of intraperitoneal tumors was appreciably decreased, and the rate of growth of subcutaneous tumors was slightly decreased in alloxanized animals, as compared with tumor-bearing controls. In tumor-bearing alloxanized animals, the high blood sugar levels characteristic of alloxan-diabetes were reduced to normal in some animals, and considerably ameliorated in others, as compared with alloxan-diabetic controls. Intraperitoneal tumors were more effective than subcutaneous tumors in reducing blood sugar levels, and relief was more pronounced in animals alloxanized just before or after transplanting than in those alloxanized two weeks before transplanting. Histological examination indicated that intraperitoneal tumor tissue invading the pancreas of alloxanized rats exerted a protective or regenerative effect on the beta-cells of the islets of Langerhans, which are selectively destroyed by alloxan. The possible role of sulphydryl groups in the amelioration of alloxan-diabetes in tumor-bearing animals, and in the reduction of tumor size and incidence in alloxan-diabetic animals is discussed. This work was reported in part in Proceedings of the American Association for Cancer Research, 2;20, 1955. / Science, Faculty of / Botany, Department of / Zoology, Department of / Graduate

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VOLUME 2 - APPENDICES

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Thesis (M.S.)--University of Wisconsin--Madison, 1982. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 54-59).

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The use of organs from donors after circulatory death (DCD) has been recommended as one strategy to enlarge the donor pool and raise the transplant rate. However, DCD allografts had higher incidence of early post-transplant dysfunction. The general aim of this research project was to develop clinical and experimental strategies to reduce the incidence of early post-transplant dysfunction of kidney and liver allografts from DCD. First the ability of a clinical scoring system based on donor data for identifying DCD kidneys with high-risk of post-transplant dysfunction was evaluated using the Oxford and the UK National DCD kidney transplant cohorts. This works suggest that stratification of DCD kidneys before transplantation might allow early identification of kidneys in which lower graft function and survival could be expected if any additional therapeutic intervention is implemented. Second, as it has been suggested that hypothermic machine perfusion (HMP) may protect DCD kidneys from additional preservation injury and improve their outcome after transplantation, this work explored the benefit of HMP as preservation technique fo DCD kidneys in Oxford and discusses the potential of this technique for reducing the incidence of post-transplant dysfunction in DCD kidneys. The Oxford. Liver Group has provided evidence of the benefit of preservation with normothermic machine perfusion (NMP) on post-transplant function and survival of DCD liver allografts. In this work, the molecular mechanisms associated with this benefit were characterized using micro array technology. This analysis suggests that the beneficial effect ofNMP may be associated with the induction of the ischaemic preconditioning phenomenon and highlights a group of genes with potential for gene therapy. Finally, this works provides the "proof-of-concept" that the use of a non-mammalian viral vector for gene transfer of kidneys and livers during conventional cold preservation is feasible and is not associated with additional tissue injury.