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Evaluating the nutritional status of Fraser fir [Abies fraseri (Pursh) Poir.] Christmas trees using foliar analysis and DRIS applicationHockman, Joseph N. January 1986 (has links)
Three studies were conducted with Fraser fir Christmas trees to determine the variation in foliar nitrogen (N) , phosphorus (P) , potassium (K), calcium (Ca), and magnesium (Mg) levels among plantation ages, sample locations within the crown, and effects due to seasonal changes. Extensive variation in nutrient concentrations existed with age of tissue collected, sample location with respect to aspect and vertical position, and normal seasonal fluctuation. Tree to tree nutrient variation indicated that greater sampling intensities are required for younger (under four years) plantations as opposed to older (over four years) plantations to achieve comparable confidence and precision levels. The data clearly illustrated that comparisons of foliar nutrient levels without regard to these sampling variables could cause erroneous diagnoses. Recommended foliar sampling practices involved sampling current-year's tissue from 2- or 3-year-old south-facing branches in October or November for routine foliar diagnoses.
Another study developed a DRIS application to evaluate N, P, K, Ca, and Mg nutrition on seventy-nine trees in a 3-year-old Fraser fir Christmas tree plantation. DRIS norms and index equations from premium grade Christmas tree were developed. In contrast to most crops where growth and yield are assessed, Fraser fir Christmas tree performance was evaluated by conventional grading procedures based on tree quality. A classification model utilizing several measured growth characteristics to predict Christmas tree grade was also investigated to objectively evaluate tree performance. Satisfactory grade-prediction results using a discriminant model were obtained yielding overall correct classification rates of 80 percent. Preliminary evaluation of DRIS performance suggested that assessments of nutritional balance, rather than examination of individual nutrient concentrations, may be more useful for prescribing fertilizer to improve Christmas tree quality. / M.S.
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The effects of phosphorus (P) deficiency on growth and nitrogen fixation of Virgilia trees from the Cape Floristic Region (CFR)Magadlela, Anathi 03 1900 (has links)
Thesis (MSc)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: The aim of this study was to determine how P nutrition affects biological nitrogen fixation (BNF) via effects on the N2-fixing bacteria in the nodules of Virgilia species native to the Cape Floristic Region (CFR), South Africa. This was evaluated in 3 separate studies:
The first study aimed to determine how phosphorus deficiency affects N nutrition of two legume tree species from the Mediterranean Fynbos ecosystem. This study showed that during prolonged P deficiency, V. divaricata maintained a constant biomass, while V. oroboides showed a decreased biomass. V. oroboides showed a decrease in nutritional concentrations, which resulted in the decrease of symbiotic nitrogen fixation (SNF). Both plants utilized atmospheric N more efficiently per nodule under P deficiency. Maximum photosynthesis decreased in V. oroboides, while V. divaricata maintained its photosynthesis. Both species also had greater carbon construction costs during P deficiency. V. divaricata showed clear adaptive features during P-deficiency, as it maintained its growth respiration. The two legume species appear to have different adaptations to P deficiency, which may influence their performance and distribution in their naturally low P environment. The second study aimed to evaluate if soil environmental conditions and mineral nutrient concentration play a major role in microbial communities in plant rhizosphere and nodulation during N2 fixation in legumes. Therefore this study firstly aimed to determine the composition of the N2 fixing bacterial population in the rhizosphere and nodules of V. divaricata. Secondly, it aimed to determine the contribution of these bacteria to N2 fixation during conditions of P deficiency in the Fynbos environment. In the study, the effects of phosphate (P) nutrition on N2 fixing bacterial community structures in Virgilia divaricata rhizosphere and nodules were examined in a pot experiment. V. divaricata were germinated in Fynbos soil as natural inoculum, transferred to clean sand cultures and supplied with 500 μM P and 5 μM P. The N2 fixing bacterial communities in the rhizosphere and nodules were examined based on the PCR-DGGE banding patterns of 16S rDNA and sequencing methods. The GenBank blast results revealed that V divaricata was efficiently nodulated by a wide range of root-nodule bacterial strains, including Burkholderia phytofirmans, Burkholderia sp. and Bradyrhizobium sp. during low P supply. The 15N natural abundance data also confirmed that 40-50% of the N nutrition was acquired through symbiotic N2 fixation. This is not only evidence of nodulation, but also an indication of the adaptation of a range of N2 fixing bacterial strains / species to the nutrient poor, sandy, acidic soil of the Mediterranean-type ecosystems of the Fynbos.
The third study examined the physiological effects, such as N2 fixation parameters, plant dependence on N2 fixation, N preference, legume plant growth, carbon costs and amino acid biosynthesis during P deficiency and mineral N supply as NH4NO3 in a slow-growing, Fynbos legume tree, Virgilia divaricata. Continued application of NH4NO3 to the legume plant showed a greater increase in plant dry matter compared to plants with two nitrogen sources (mineral N and atmospheric N2) or plants that relied on atmospheric N2 fixation. Carbon construction costs were more pronounced in plants supplied with two N sources and during P deficiency. Maximum photosynthetic rates per leaf area increased during prolonged P deficiency, irrespective of the N sources. Though plants nodulated, plant dependence on N2 fixation decreased with the addition of NH4NO3. Roots and nodules of the P deficient plants showed an increase in asparagine content, most strikingly so in plants treated with a single source of N. These studies reveal that different legume species of the same genus, may employ contrasting adaptations in order to maintain N nutrition under P deficiency. / AFRIKAANSE OPSOMMING: Die doel van hierdie studie was die bepaling van die wyse waarop fosfaat (P) voeding die biologiese stikstof binding (BNF) deur middel van die effek op N2-bindingsbakterië in die wortelknoppies van Virgilia spesies wat inheems tot die Kaap floraryke area (CFR), Suid Afrika is, affekteer. Drie aparte eksperimente is uitgevoer om die doel te evalueer:
Die eerste studie het gepoog om te bepaal hoe 'n fosfaat tekort N voeding van twee peulplant spesies van die Mediterreense Fynbos ekosisteem affekteer. Hierdie studie het getoon dat V. divaricata 'n konstante biomassa tydens verlengde P tekort behou, terwyl V. oroboides ‟n verlaagde biomassa getoon het. V. oroboides het 'n verlaging in voedingskonsentrasies getoon, wat tot 'n verlaging in simbiotiese stikstof binding (SNF) gelei het. Beide plante benut atmosferiese N meer doeltreffend per nodule tydens P tekort. Die maksimum fotosintese in V. oroboides het afgeneem, terwyl V. divaricata sy fotosintese gehandhaaf het. Beide spesies het ook 'n groter koolstof konstruksie koste tydens P tekort gehad. V. divaricata toon duidelike aanpassingsmeganismes tydens P-tekort, aangesien hierdie species sy groei respirasie konhandhaaf. Dit wil voorkom asof die twee peulplant spesies verskillend aangepas is vir P tekort, wat hulle producksie en verspreiding in hulle natuurlike lae P omgewing mag beïnvloed. Die doel van die tweede studie was om te bepaal of grond omgewingskondisies en minerale voedingskonsentrasie 'n belangrike rol speel in die mikrobiese gemeenskappe in die plant risofeer en wortelknoppie vorming tydens N2 binding in peulgewasse. Eerstens het die studie dus gepoog om die samestelling van die N2 bindende bakteriële populasie in die risosfeer en die wortelknoppies van V. divaricata te bepaal. Ten tweede, is die bydrae van die bakterië tot N2-binding tydens P tekort kondisies in die Fynbos omgewing bepaal. In die studie is die effek van fosfaat (P) voeding op die N2-bindende bakteriële gemeenskapstrukture in die Virgilia divaricata risofeer en wortelknoppies in 'n pot eksperiment ondersoek. V. divaricata sade is in fynbos grond as 'n natuurlike inokulum ontkiem, waarna dit na skoon sand kulture oorgedra is en van 500 μM P en 5 μM P voorsien is. Die N2-bindende bakteriële gemeenskappe in die risofeer en wortelknoppies is op grond van die PCR-DGGE band patrone van die 16S rDNA en volgorde bepalingsmetodes ondersoek. Die GenBank Blast resultate het getoon dat V. divaricata doeltreffend deur 'n wye reeks wortel-wortelknoppie bakteriële stamme genoduleer is, insluitende insluitende Burkholderia phytofirmans, Burkholderia sp. en Bradyrhizobium sp. tydens lae P toediening. Die natuurlike 15N voorkoms data het ook bevestig dat 40-50% van die N voeding deur simbiotiese N2 binding bekom is. Dit dien nie net as bewys vir wortelknoppie vorming nie, maar ook 'n aanduiding van die aanpassing van 'n reeks N2 bindende bakteriële stamme/ spesies tot die voedingsarme, sanderige, suur grond van die Mediterreanse ekosisteem van die Fynbos.
Die derde studie het die fisiologiese effekte soos bv. N2 fikserings faktore, die afhanklikheid van plante op N2 fiksering, N voorkeur, peulgewas groei, koolstof kostes en aminosuur biosintese tydens P tekort en minerale N toediening soos NH4NO3 in 'n stadig-groeiende, Fynbos peulgewasboom, Virgilia divaricata ondersoek. Volgehoue toediening van NH4NO3 aan die peulplant toon 'n groter toename in plant droë weefsel. Tydens P tekort is die koolstof bou koste meer verhoog in plante wat met twee N bronne voorsien is. Tydens verlengde P tekort het die maksimum fotosintese tempo per blaaroppervlakte toegeneem, ongeag die N bron. Alhoewel die plante wortelknoppies gevorm het, het die plant se afhanklikheid van N2 binding tydens die toediening van NH4NO3 afgeneem. Wortels en wortelknoppies van die P tekort plante het 'n toename in asparagien inhoud getoon, veral in die plante wat met 'n enkele bron van N behandel is. / The DST/NRF-Center of Excellence for Tree Health and Biotechnology, based at the University of Pretoria, for their financial support
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Nutrient management strategies for nectarine orchardsKrige, Graeme Tertius 12 1900 (has links)
Thesis (MScAgric)--University of Stellenbosch, 2007. / ENGLISH ABSTRACT: The macro-element uptake and distribution by higher density central leader
‘Donnarine’ nectarine trees was studied through the sequential excavation of trees. A
large portion, 41.5%, of the nitrogen manifested in the new growth from dormancy up
to pit-hardening, originated from nitrogen reserves in the permanent structure. The
permanent structure was also an important source of phosphorous reserves. Of the
phosphorous in the fruit, leaves and new shoots at pit-hardening, 35.0% came from
reserves in the permanent structure. Potassium did not act as an important reserve in
the nectarine trees and was taken up throughout the season. From pit hardening to
harvest the fruit represented the most important sink for potassium. Calcium and
magnesium, like potassium, did not play significant roles as reserves in the nectarine
tree and must be available for uptake from the beginning of the season for new growth
and development as well as fruit quality.
The micro-element uptake and distribution was also studied through the sequential
excavation of the same ‘Donnarine’ nectarine trees. Little scientific data is available
on this topic. Manganese and iron was found to act as important reserves in the tree
with 46.2% of manganese and 59.5% of the iron found in the new growth at pithardening
coming from reserves translocated from the permanent structure. Zinc and
boron reserves also play a role in nectarine trees, but to a lesser extent than
manganese and iron.
The seasonal mineral nutrient demand of the same ‘Donnarine’ nectarine trees was
determined through the sequential excavation of trees and losses and fixation was
calculated. Guidelines regarding nutritional requirements per ton of fruit produced per
hectare by higher density nectarine orchards are respectively 3.82kg nitrogen, 0.35kg
phosphorous, 4.43kg potassium, 1.53kg calcium, 0.52kg magnesium, 32.45g sodium,
9.44g manganese, 37.46g iron, 3.24g copper, 13.95g zinc and 10.52g boron. Sodium
is not commonly considered to be essential to higher plants, but was included in the
trial.
Nutrient solutions with four different EC (electrical conductivity) levels were applied
to ‘Donnarine’ nectarine trees under pulsating drip fertigation for three periods of different lengths, before harvest. Raising the nutrient solution EC to positively affect
fruit quality is a technique widely utilised in the vegetable industry. This technique
did, however, not have similar positive effects on nectarine fruit grown under a
pulsating drip fertigation system. Good production practices such as accurate nutrition
and irrigation as well as the correct horticultural inputs should be the primary focus of
producers who wish to alter or improve the fruit quality of their crop. / AFRIKAANSE OPSOMMING: Voedingbestuur strategieë in nektarien boorde
Die makro-element opname en verspreiding deur hoër digtheid sentrale leier
‘Donnarine’ nektarien bome is bestudeer d.m.v. opeenvolgende opgrawings van
volledige bome en die ontleding van monsters. ‘n Groot hoeveelheid, 41.5%, van die
stikstof wat tydens pitverharding in die nuwe groei teenwoordig was, is d.m.v.
translokasie vanuit die permanente struktuur van die boom afkomstig. Die permanente
struktuur was ook ‘n belangrike bron van fosfaat reserwes. Teen pitverharding was
35.0% van die fosfaat in die nuwe groei afkomstig vanuit die permanente struktuur.
Bevindings het getoon dat kalium nie as ‘n reserwe in die nektarien bome opgetree het
nie en dié element is deur die groeiseisoen opgeneem. Vanaf pitverharding tot en met
oestyd was die vrugte die sterkste setel van aanvraag vir kalium. Kalsium en
magnesium het, soos in die geval van kalium, nie ‘n belangrike rol as reserwe vertolk
nie. Beskikbaarheid van hierdie elemente vir opname vanaf die begin van die
groeiseisoen is dus baie belangrik vir nuwe groei en ontwikkeling asook
vrugkwaliteit.
Die mikro-element opname en distribusie van dieselfde ‘Donnarine’ nektarien bome
is ook bestudeer d.m.v. opeenvolgende opgrawings en analise van volledige bome.
Min wetenskaplike literatuur oor hierdie onderwerp is beskikbaar. Bevindings het
getoon dat mangaan asook yster baie belangrike reserwes in die nektarien boom is.
Tydens pitverharding was 46.2% van die mangaan en 59.5% van die yster wat in die
nuwe groei teenwoordig was, afkomstig vanaf reserwes uit die permanente struktuur
van die boom. Verder het sink en boor ook as reserwes opgetree, maar tot ‘n mindere
mate as mangaan en yster. Die seisoenale behoeftes aan minerale voeding van dieselfde ‘Donnarine’ nektarien
bome is bepaal d.m.v. opeenvolgende opgrawings en analise van volledige bome
asook die bepaling van verwyderingsverliese en vaslegging. Voedingsriglyne is
vasgestel i.t.v. die hoeveelheid voedingstof wat per hektaar benodig word om een ton
nektariens te produseer. Die riglyne is as volg: 3.82kg stikstof, 0.35kg fosfaat, 4.43kg
kalium, 1.53kg kalsium, 0.52kg magnesium, 32.45g natrium, 9.44g mangaan, 37.46g
yster, 3.24g koper, 13.95g sink en 10.52g boor. Natrium word nie in die algemeen as
‘n essensiële plantvoedingselement beskou nie, maar is by die berekeninge ingesluit. Voedingsoplossing met vier verskillende vlakke van EG (elektriese geleiding) is vir
drie periodes van verskillende lengtes aan ‘Donnarine’ nekarien bome toegedien. Die
verhoging van die EG van voedingsoplossings ten einde kwaliteit te verbeter is ‘n
tegniek wat met groot suskses in die groentbedryf toegepas word. Hierdie tegniek het
egter nie soortgelyke positiewe effekte op die nektarien vrugkwaliteit gehad nie.
Produsente wat hul vrugkwaliteit wil verbeter behoort primêr te fokus op goeie
produksiepraktyke soos akkurate voeding en besproeiing asook die korrekte
tuinboukundige insette.
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Patterns, mechanisms, and implications of spatial variability in the ecological processes regulating nutrient access by forest treesAkana, Palani Robert January 2022 (has links)
The processes that regulate nutrient access by forest trees exhibit substantial variability on both large and small spatial scales. Explicit study of this spatial variability promotes a better understanding of the structure and function of forests. While the importance of space in ecological processes is being increasingly appreciated, there are major gaps in our knowledge about how space influences plant nutrient supply, particularly within a forest stand. This dissertation consists of three chapters that examine the patterns, drivers, and implications of spatial variation in three main processes that make nutrients available to trees: throughfall nutrient deposition, soil nutrient mineralization, and root system development.
In Chapter 1, I use data from a field experiment to examine the effect of fertilization on nutrient transfer from the canopy to the soil via throughfall and litterfall in a tropical rainforest. I demonstrate that at small spatial scales, canopy density controls the flux of nutrients in throughfall, while at large scales, soil fertility is an important control, especially for phosphorus. I also show throughfall can be as important as litterfall in the recycling of certain essential nutrients like potassium, and depending on soil fertility, phosphorus.
In Chapter 2, I investigate the small scale spatial patterning in soil nitrogen, a nutrient that frequently limits tree growth, in a temperate forest. By quantifying the degree of spatial inequality and autocorrelation in two plots characterized by different dominant tree species, I show that soil extractable nitrogen pools and net nitrogen mineralization fluxes exhibit a high degree of spatial patterning at scales less than 5 meters, with a majority of nitrogen availability contained within hotspots comprising a small proportion of soil area. I also demonstrate that this spatial patterning affects seedling access to soil nitrogen, which has consequences for seedling growth and survival.
Chapter 3 examines how tree species and tree size affect the spatial distributions of root systems in two temperate tree species and explores how differences in root spatial coverage could affect tree nutrient access. I find that the spatial distributions of tree root systems can exhibit dramatic differences between species, with a tradeoff between root spatial coverage and total root length. I also discover that the effect of root spatial coverage on soil nutrient access is highly dependent on the spatial patterning of the soil nutrient, such that tree access to patchy nutrients varies greatly based on tree location within the local soil environment, even for medium-size trees. Together, these chapters characterize important patterns and mechanisms of spatial variation in the processes that regulate tree nutrient access.
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Nutrient requirement and distribution of intensively grown ‘Brookfield Gala’ apple treesKangueehi, Grace Nandesora 03 1900 (has links)
Thesis (MscAgric (Horticulture))--University of Stellenbosch, 2008. / ‘Brookfield Gala’ apple trees were planted out in July 2003 in a Dundee soil form, consisting
of well-aerated sandy loam soil. During the first 12 months trees received young tree
solutions high in nitrogen. The nutrient solution of the 2nd leaf trees was based on a yield
estimation of 10 ton. ha-1 plus 30%. Nutrient solutions for the 3rd leaf trees were based on 25
ton. ha-1 yield estimations and adapted upwards.
Seasonal uptake and distributions were determined for macro and micro elements, using twoand
three-year-old apple trees during the seasons 2004/2005 and 2005/2006. In the bearing
apple trees the macro nutrient accumulated rapidly from late winter to late autumn. Prior to
leaf drop most of the N, P, S, Mg and a small portion of K were redistributed back into the
permanent parts of the tree. On the other hand, all Ca in the leaves was lost through leaf drop.
Apple fruit contains comparatively large quantities (±60.2%) of K, which are removed during
harvest.
Guidelines for minimum and maximum nutritional requirements based on the amount
necessary to produce 1 kg fruit were determined. For the 3rd leaf trees the minimum macro
nutrient requirements (g. kg-1 yield) of N, P, K, Ca, Mg and S were ±1.7, ±0.3, ±2.3, ±0.5,
±0.2 and ±0.2, respectively. The maximum nutrient requirements (g. kg-1 yield) for N, P, K,
Ca, Mg and S were ±2.6, ±0.4, ±3.3, ±1.9, ±0.4 and ±0.2, respectively. For the 3rd leaf trees
the minimum micro nutrient requirements (mg. kg-1 yield) of Na, Mn, Fe, Cu, Zn, B and Mo
were ±75.1, ±1.3, ±28.7, ±0.9, ±3.0, ±5.7 and ±0.3, respectively. The maximum nutrient
requirements (mg. kg-1 yield) of Na, Mn, Fe, Cu, Zn, B and Mo were ±102.9, ±7.8, ±32.6,
±1.1, ±6.5, ±7.6 and ±0.3, respectively.
Labelled N uptake and distribution for two- and three-year-old apple trees were also
determined during the same seasons. The labelled N uptake and distribution results indicated
that there was a low labelled N uptake in the initial growth stages, suggesting the importance
of internal N reserves for plant development at the beginning of the season. In the active
growing period more than 60% of the labelled N was found in the new growth. Uptake
efficiency improved as the trees grew older. The effect of different nutrient levels on tree growth, yield and fruit quality was assessed:
lower (80%) than the standard (100%) and three higher (120%, 140% and 160%). Results
indicated that different nutrient levels had no effect on yield, blush or TSS during the 18
months of application over two bearing seasons. The application of biological products
(humic acid, and compost plus compost extract) over a period of 18 months had a significant
influence on the TSS, malic acid and citric acid concentrations. A tendency towards an
increase in total fine root number and length occured with the addition of biological
ameliorant.
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