Made available in DSpace on 2014-07-29T15:30:36Z (GMT). No. of bitstreams: 1 dissertacaopatriciabreu.pdf: 1725826 bytes, checksum: d77d7164c903bff7a0df515bcc455897 (MD5) Previous issue date: 2008-12-17 / This study evaluated the frequency of Trichomonas vaginalis infection in human immunodeficiency virus positive (HIV+) and negative (HIV-) women in Goiania, Goiás, Brazil, comparing the presence of the parasite in the two groups and correlating it with the conditions of immunodeficiency present in these women. The diagnostic techniques used, wet mount microscopy, culture and cytology, were also evaluated, and the principal inflammatory alterations in the two groups were assessed. The HIV+ samples (test group) were collected at the Hospital of Tropical Diseases and in the Maternal and Child Healthcare Hospital, whereas the HIVnegative samples (control group) were collected at the Maternity Hospital. Swabs were used for wet mount microscopy (saline solution) and for culture (Diamond s medium), and Ayre s spatula and brush were used for the cytology smears, which were fixed using a commercial fixative. A total of 237 samples were analyzed, 125 HIV-positive test samples and 112 HIV-negative controls. The overall frequency of T. vaginalis was 13.9%, 18.4% in the HIV+ and 8.9% in the HIV- group. This difference was statistically significant (p<0.05); however, the infection was not associated with immunodeficiency according to CD4, viral count and lymphocytes. There was a significant difference in the prevalence of the parasite between HIV+ and HIVpregnant women (22.6% versus 12.5%). Culture identified a frequency of T. vaginalis of 13.9%, while cytology identified a rate of 13.5% and wet mount microscopy 11.4%. Perinuclear halos were the most frequent inflammatory alteration; however, there was no difference between the groups / O estudo avaliou a freqüência da infecção por Trichomonas vaginalis em mulheres HIV+ (vírus imunodeficiência humana) e HIV- em Goiânia-GO, comparando a presença do parasito e correlacionando com as condições de imunodeficiência. Avaliou também as técnicas de diagnóstico: exame a fresco, cultura e citologia, e apontou as principais alterações inflamatórias nos dois grupos. As amostras de HIV+ (grupo teste) foram coletadas no Hospital de Doenças Tropicais e no Hospital Materno Infantil e as de HIV negativas (grupo controle) na Maternidade Nascer Cidadão. Foram utilizados swabs para os exames a fresco (salina) e para a cultura (meio Diamond), espátula de Ayre e escovinha para os esfregaços citológicos que foram submetidos a fixadores comerciais. Foram examinadas 237 amostras: 125 do teste e 112 do controle. A freqüência por T. vaginalis foi 13,9%, sendo 18,4% nas HIV+ e 8,9% nas HIV-. O resultado foi estatisticamente significativo (p<0,05), porém a infecção não foi associada à imunodeficiência (CD4, carga viral e linfócitos). Houve diferença significativa entre grávidas HIV+ e HIV- (22,6% vs 12,5%). A Cultura obteve 13,9% da presença de T.vaginalis, a Citologia 13,5% e o exame a fresco 11,4%. Halos perinucleares predominaram na avaliação das alterações inflamatórias, porém não houve diferença entre os grupos.