The goal of my thesis "Beginnings and history of state interventions into housing market using Czech market as example" is to illustrate circumstances (conditions) in which housing policy started to evolve. Next goal is to explain the reasons why state interventions on Czech housing market grew stronger. Historical circumstances, which were important in housing market shaping, are accompanied by analysis of historical sources. First part of thesis is focused on explaining basic terms regarding forming housing market and highlighting specialties of housing as (tady ten statek nevím). Second part demonstrates first state interventions in Czech Republic territory using historical legislative sources, statistical data and historical context. It starts in Austria-Hungary and then chronologically moves over two World Wars era and socialism to the present.

In animals, some of the most critical regulators of gene expression are nuclear hormone receptors (NRs) and their coregulators, specifically the Mediator complex. Of particular interest are the NRs implicated in metabolic and developmental regulation and in carcinogenesis: thyroid hormone receptors (TRs) and retinoid X receptors (RXRs). In this work, I venture to elucidate some aspects of gene expression regulation by these NRs: the degree of evolutionary conservation of signalling based on NRs and their coregulators; the mechanisms of negative regulation by NRs; and possible implications of these findings for clinical medicine. State-of-the-art bioinformatical, genome editing and microscopic techniques are applied at three levels of animal evolution to study NRs and Mediator. Reverse genomics in human patients suffering from the syndrome of resistance to thyroid hormones β are used to infer the structure and function of TRβ subdomains. Alignments, binding studies and in vivo experiments in Trichoplax adhaerens allow identification of a close orthologue of human RXR at the basis of metazoan evolution. Employing database queries, genome editing and microscopy, we describe a correct orthologue of the Mediator subunit 28 in Caenorhabditis elegans, indicating a complete homology of the Mediator complex...

Enterprise product, in our case either an embedded system or a software application, development is a complex task, which require model approaches and multiple diverse software tools to be linked together in a tool-chain to support the development process. Individual tools in the tool-chain maintains an incomplete picture of the development process. Data integration is necessary between these various tools in order to have a unified, consistent view of the whole development process. Information integration between these tools is a challenging task due to heterogeneity of these tools. Linked data is a promising approach for tool and data integration, where tools are integrated at data level in a tool-chain. Linked data is an architectural style to integrate and require more definitions and specifications to capture relationships between data. In our case, data of tools are described and shared using OSLC specifications. While such an approach has been widely researched for tool integration, but none covers the aspect of using such a distributed approach for lifecycle data integration, management and search. In this thesis work, we investigated the use of linked data approach to lifecycle data integration. The outcome is a prototype tool-chain architecture for lifecycle data integration, which can support data intensive queries that require information from various data sources in the tool-chain. The report takes Scania´s data integration needs as a case study for the investigation and presents various insights gained during the prototype implementation. Furthermore, the report also presents the key benefits of using a linked data approach for data integration in an enterprise environment. Based on encouraging test results for our prototype, the architecture presented in this report can be seen as a probable solution to lifecycle data integration for the OSLC tool - chain. / Företagets produkt, i vårt fall antingen ett inbyggt system eller ett program, är utvecklingen en komplicerad uppgift, som kräver modell metoder och flera skiftande programverktyg som ska kopplas samman i en verktygskedja för att stödja utvecklingsprocessen. Enskilda verktyg i verktygskedjan bibehåller en ofullständig bild av utvecklingsprocessen. Dataintegration är nödvändigt mellan dessa olika verktyg för att få en enhetlig och konsekvent syn på hela utvecklingsprocessen. Informationsintegration mellan dessa verktyg är en utmanande uppgift på grund av heterogenitet av dessa verktyg. Kopplad data är en lovande strategi för verktygs-och dataintegration, där verktyg är integrerade på datanivå i en verktygskedja. Kopplade uppgifter är en arkitektonisk stil att integrera och kräver fler definitioner och specifikationer för att fånga relationer mellan data. I vårt fall är data av verktyg beskrivna och delad med hjälp av OSLC specifikationer. Medan ett sådant tillvägagångssätt har i stor utsträckning forskats på för integrationsverktyg, men ingen täcker aspekten att använda en sådan distribuerad strategi för livscykeldataintegration, hantering och sökning. I detta examensarbete, undersökte vi användning av länkad data strategi för livscykeldataintegration. Resultatet är en prototyp av verktygskedjans arkitektur för livscykeldataintegration, som kan stödja dataintensiva frågor som kräver information från olika datakällor i verktygskedjan. Rapporten tar Scanias dataintegrationsbehov som en fallstudie för utredning och presenterar olika insikter under genomförandet av prototypen. Vidare presenterar rapporten också de viktigaste fördelarna med att använda en länkad-data-strategi för dataintegration i en företagsmiljö. Baserat på positiva testresultat för vår prototyp, kan arkitekturen presenteras i denna rapport ses som en trolig lösning för livscykeldataintegration för OSLC verktyg - kedja.

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2016-09-19T15:16:21Z No. of bitstreams: 1 2010 - Juarez Vicente.pdf: 1012625 bytes, checksum: 30ce03687174fc4cf4226e455adde13a (MD5) / Made available in DSpace on 2016-09-19T15:16:21Z (GMT). No. of bitstreams: 1 2010 - Juarez Vicente.pdf: 1012625 bytes, checksum: 30ce03687174fc4cf4226e455adde13a (MD5) Previous issue date: 2010-05-07 / From the scientific viewpoint, the word ?uncertainty? expresses doubt about an analytic result. The implementation of the concept of uncertainty of measurement is a crucial step that the Brazilian laboratories must take in the process for obtaining the certification of the ability to perform essays in accordance with the Brazilian technical norm ABNT NBR ISO/IEC 17025. Regardless the technical area of application, the determination of the uncertainty of measurement associated with the result of an analysis is extremely important, especially in the food industry. The expanded uncertainty is related with the degree of reliability of an analytical result, and by means of this information it is possible to evaluate if the result of an analysis is consistent, i.e., if the uncertainty associated to the measurement remains inside an acceptable range. When the percentage of uncertainty exceeds a threshold, it is necessary to find the process variables that more intensely contribute to increasing the uncertainty and, in the sequence, to take corrective actions in order to minimize the impact caused for those variables over the uncertainty of measurement. The aim of this study was to detect the uncertainty of measurement associated with the analysis of total reducing sugars (TRS) by the Lane-Eynon method. The equipments used were analytical and semi analytical balances, burettes with 10mL and 25mL, and the food matrix studied was wild honey. To perform the calculations of the standard uncertainty, u, information about the uncertainty of the laboratory materials used in the analysis ? obtained from the certificate of calibration. The combined uncertainty, uc, was obtained by deriving the influence factors and considering, if necessary, the effective degree of freedom, ?eff, as stated in the Welch-Satterthwaite equation. The expanded uncertainty, U, was obtained by multiplying uc by the appropriate coverage factor, k = 2 (at level of confidence of 95,45%). The results showed that the expanded uncertainty obtained for the analysis of TRS was less than 2,0% when the analytical balance was used. The process variable having the greatest impact was the volume (close to 80,0%), followed by the title (close to 20,0%). The analysis of the expanded uncertainty obtained for the analysis of TRS when the semi analytical balance, and 10mL as well as 25mL burettes were used provided reliable results. However, care must be taken in such a combination of equipments, especially when using the 25mL burettes, since the percentage of uncertainty was great than 3,5%, too close to the limit established by the Codex Alimentarius, 4,0%. It was observed that, in this case, the variable having the greatest impact on the uncertainty was the title, with 79,82% (10mL burettes), and 87,10% (25mL burettes). / Do ponto de vista t?cnico-cient?fico, a palavra ?incerteza? expressa a d?vida em rela??o a um resultado anal?tico. A aplica??o do conceito de incerteza de medi??o ? fundamental para os laborat?rios brasileiros que almejam obter o reconhecimento da capacidade de realiza??o de ensaios em conformidade com a norma ABNT NBR ISO/IEC 17025. Independentemente da ?rea de atua??o, ? de extrema import?ncia o estudo e o conhecimento da incerteza de medi??o associada a um resultado de an?lise, em particular quando aplicado ? ind?stria de alimentos. A incerteza expandida informa o grau de confiabilidade de um resultado anal?tico e, atrav?s dela, ? poss?vel avaliar, a um n?vel de abrang?ncia estabelecido, se o resultado de uma an?lise transmite credibilidade, ou seja, se o valor da incerteza est? em uma faixa de concentra??o aceit?vel ou n?o. Quando o percentual de incerteza ultrapassa o limite aceit?vel, ? necess?rio conhecer quais as vari?veis do m?todo que mais contribuem para a incerteza e executar as medidas corretivas a fim de minimizar este impacto e, consequentemente, reduzir a incerteza total. O objetivo geral deste trabalho foi determinar a incerteza expandida associada ? an?lise titulom?trica de a??cares redutores totais (ART) pelo m?todo de Lane-Eynon, tendo como matriz uma amostra de mel, utilizando balan?a anal?tica e semi anal?tica e buretas de 10mL e 25mL. Para o c?lculo da incerteza padr?o (u), utilizaram-se os dados obtidos nos certificados de calibra??o de equipamentos e vidrarias empregados na an?lise. A incerteza combinada ( ) foi obtida derivando os fatores de influ?ncia e considerando, quando necess?rio, o grau de liberdade efetivo (?eff), dado pela Equa??o de Welch-Satterthwaite. Obteve-se a incerteza expandida (U) multiplicando-se por 2 (dois) o valor de para um n?vel de confian?a de 95,45%. Os resultados obtidos mostraram que a incerteza expandida de ART utilizando balan?a anal?tica n?o foi significativa (<2%) e que a vari?vel que mais influenciou foi o volume de amostra gasto (~80%), seguido do T?tulo (~20%). A an?lise da incerteza expandida de ART obtida com balan?a semi anal?tica e buretas de 10mL e 25mL gerou resultados confi?veis. No entanto, deve haver cautela neste caso, especialmente ao se utilizar bureta de 25mL, visto que o valor percentual de incerteza superou 3,5%. Observa-se que a vari?vel que mais contribui para a incerteza foi o T?tulo com 79,82% (bureta de 10mL) e 87,10% (bureta de 25mL).

No description available.

Approved for public release; distribution is unlimited. / This paper documents the development of a statistical analysis package for the TRS-80 microcoraputer. The package is comprised of six interactive programs which are generally divided into topical areas. The major emphasis is on exploratory data analysis and statistical inference, however, probability and inverse probability distributions are also included. The programming language is TRS-80 Level II BASIC enhanced by the input/output commands available through the ESF-80 (Exatron Stringy Floppy) mass storage subsystem. With the modification of these few commands, the package is compatible with most floppy disk operating systems designed for the TRS-80 Model I or Model III microcomputers. This statistical analysis capability implemented on a relatively inexpensive system provides a useful tool to the student or the trained analyst without ready access to a mainframe computer system. / Major, United States Marine Corps

A phylogenetic tree based on ITS1, Adh1 and ndhF grouped the species of the genus Sorghum into one distinct monophyletic group, but including two sister lineages, one with x=5, the other with x=10 as basic chromosome numbers. The goal of this study was to elucidate major patterns in Sorghum genome evolution, particularly n=5 vs. n=10 genomes. A very recent molecular cytogenetic study in our laboratory revealed striking structural karyotypic rearrangements between S. bicolor (x=10) and an x=5 Sorghum species, S. angustum; so an immediate objective here was to determine if identical or similar rearrangements exist in other wild Sorghum species. Our approach was [1] to extend similar methods to additional species, i.e., fluorescent in situ hybridization (FISH) analyses of sorghum genomic bacterial artificial chromosome clones and multi-BAC cocktail probes to mitotic chromosomes of S. angustum, S. versicolor, S. brachypodum and S. intrans; and [2] to augment the BAC-FISH findings by comparing telomeric and ribosomal DNA FISH signal distributions to x=5 and x=10 Sorghum species. Signals from in situ hybridizations of BAC-based probes were insufficiently robust and insufficiently localized to delineate FISH signal patterns akin to those discovered previously in S. angustum. Southern blots of the same BACs to restricted DNA of these species revealed relatively moderate affinity to smeared DNA, suggesting homology to non-tandemized sequences. FISH of the A-type TRS (Arabidopsis-like telomeric repeat sequence) revealed its presence is limited to terminal chromosomal regions of the Sorghum species tested, except S. brachypodum, which displayed intercalary signal on one chromosome and no detachable signal at its termini region. The hybridization of 45S and 5S rDNA revealed that the respective sites of tandemized clusters differ among species in terms of size, number and location, except S. angustum versus S. versicolor. Well localized BAC-FISH signals normally occur when signals from low-copy sequences discernibly exceed background signal, including those from hybridization of dispersed repetitive elements. The low level of signal intensity from BAC low-copy sequences relative to the background signal "noise" seems most likely due to low homology and(or) technical constraints. Extensive dispersal of low-copy sequences that are syntenic in S. bicolor seems unlikely, but possible. In conclusion, the result was a lack of clear experimental success with BAC-FISH and an inability to effectively screen for S. angustum-like rearrangements using BAC-FISH. The telomeric and rDNA FISH indicated that the x=5 genomes vary extensively. One can surmise that although the arrangements seen in S. angustum might extend to S. versicolor, they certainly do not extend to S. versicolor, they certainly do not extend to S. intrans or S. brachypodum. It is clear that S. brachypodum has telomeric repeats that are either very short or rely on some sequence other than the A-type TRS.

A local integrated pulp and paper Kraft mill had come under pressure from the local communities and mill personnel to reduce the odours that were perceived to be generated at the Farm Dams and irrigation farm situated adjacent to the mill. The typical odours associated with Kraft mills are due to the generation of four reduced sulphur compounds such as hydrogen sulphide (H2S), methyl-mercaptan (CH3SH), dimethyl-sulphide (CH3)2S and dimethyl-disulphide (CH3)2S2. These compounds are collectively referred to as Total Reduced Sulphur (TRS) components which are generated as a direct result of the Kraft pulping and chemical recovery process. These components can either be in the gaseous or aqueous phase depending on the characteristics of the effluent. Gaseous and aqueous TRS profiling of the mill indicated that hydrogen sulphide (H2S) was the main odour component generated and emitted from the Clarifiers and the Treated Effluent Transfer Sump (TETS) at the effluent treatment plant. The hydrogen sulphide (H2S) emission levels were affected by process upsets, sludge removal frequencies, chemical composition of the effluent, Sulphate Reducing Bacteria (SRB) activity, pH and temperature fluctuations. Treatment options such as pH control using slaked lime, dosing of biocides, addition of biomodifiers and/or a sulphate reduction inhibitor were investigated. The use of slaked lime, Ca(OH) 2, for pH control was not practical due to continuous pH fluctuations, increasing the pH would increase the scaling tendencies of the effluent and would also affect the soil cation-anion exchange properties of the irrigated farm land. The use of non-oxidising biocides was effective in reducing SRB activity between 99.2% and 99.8% at dosages between 4 mg/l and 25 mg/l. However, the use of biocides was not considered as a long term treatment option due to the various disadvantages such as the stability of the biocides at fluctuating pH and temperatures, half-life, environmental accumulation, toxicity and costs. The aqueous H2S level was reduced by 79% using different combinations of biomodifiers (nitrates, nitrites, molybdenum). Increasing the dosages of the biomodifiers (> 500mg/l) would be required to increase the reduction of H2S levels by more than 79%. The increased dosages would significantly increase the cost of the treatment programme. The accumulation of nitrates, nitrites and molybdenum could affect the soil texture, cation-anion exchange capacity, permeability, Sodium Absorption Ratio (SAR) and nutrient availability. A more environmentally friendly and cost effective treatment was found using sodium nitrate (biomodifier) together with AQ (sulphate reduction inhibitor). The continuous dosing of 50 mg/l sodium nitrate together with 4 mg/l AQ would be effective in reducing the average aqueous H2S levels (40 mg/l) by at least 92%. This treatment would also be compatible with aeration or oxidation procedures to further increase the removal of H2S to achieve an aqueous H2S level of <1 mg/l. Aeration or oxidation would also increase the dissolved oxygen and COD levels, increase the inhibition of SRB activity and oxidise any reduced sulphur. The dosing of sodium nitrate and AQ to control the generation of H2S is not patented in South Africa. It can, therefore, be used to treat the Kraft mill effluent without violating any intellectual property rights in South Africa. / Dissertation (MSc(Applied Science))--University of Pretoria, 2008. / Chemical Engineering / unrestricted

Sabe-se que o hipotireoidismo (Hipo) resulta em supressão do eixo hormônio de crescimento (GH)/ insulin-like growth factor I (IGF-I) e em atraso no desenvolvimento esquelético. Em um estudo anterior, vimos que o tratamento de ratas jovens Hipo com GC-1, um análogo da triiodotironina (T3) seletivo pela isoforma <font face=\"symbol\">b de receptor de hormônio tireoideano (TR<font face=\"symbol\">b), não teve efeito sobre o IGF-I sérico ou sobre a expressão protéica de IGF-I nas lâminas epifisiais, mas parcialmente reverteu alterações esqueléticas decorrentes do Hipo, o que sugere que: (i) o desenvolvimento esquelético requer ações do T3 mediadas pelo TR<font face=\"symbol\">a1 e TR<font face=\"symbol\">b1 (isoformas de TR expressas no osso); ou (ii) requer interações entre o eixo GH/IGF-I e o hormônio tireoideano. Neste estudo, investigamos essas hipóteses tratando ratas recém desmamadas Hipo com T3 ou GC-1 em associação ou não com o GH por 4 semanas. Os nossos achados mostram que o T3 e GH interagem para promover o desenvolvimento ósseo, mas que uma série de efeitos do T3 nesse processo independe do eixo GH/IGF-I e são mediadas pelo TR<font face=\"symbol\">a e/ou TR<font face=\"symbol\">b. / Thyroid hormone (TH) has important effects on bone development and metabolism. It is known that triiodotyronine (T3) has indirect actions in the skeleton through its influence on the production and secretion of growth hormone (GH)/ insulin-like growth factor (IGF-I) and/or other factors. On the other hand, direct actions of T3 on bone are recognized but not yet clear. Most of T3 action is mediaded by its nuclear receptors (TRs). TR<font face=\"symbol\">a1, TR<font face=\"symbol\">b1 e TR<font face=\"symbol\">b2 bind T3, while TR<font face=\"symbol\">a2 does not bind T3 and acts as an antagonist of genic transcription of TR<font face=\"symbol\">a1 and TR<font face=\"symbol\">b1. All these receptors, except TR<font face=\"symbol\">b2, are expressed in chondrocytes of growth plate, osteoblasts and osteoclastos. However, the functional roles of each TR isoformas in the bone development are incompletely understood. A few years, it is development GC-1, a synthetic analog of T3 which is selectivwe for TR<font face=\"symbol\">b1 over TR<font face=\"symbol\">a1. In recent study, we showed that treatment of hypothyroid young rats with T3 revert the IGF-I deficiency and skeleton defects caused by hypothyroidism. Since GC-1 treatment does not effects on serum levels of IGF-I or protein expression of IGF-I in the growth plate, but revert some bone alterations induced by T3 deficiency. Considering the selectivity of GC-1 for TR<font face=\"symbol\">b, these findings suggest that T3 has effects on bone development that are mediated by TR<font face=\"symbol\">b and independent of GH/IGF-I axis. On the other hand, the inability of GC-1 in completely revert the alterations of bone development suggests that the normal skeleton development require (i) T3 actions mediated by TR<font face=\"symbol\">a1 and TR<font face=\"symbol\">b1, or (ii) synergic or additive actions between GH/IGF-I axis and thyroid hormone. To investigate these hypotheses, 21 day-old hypothyroid female rats were treated with T3 or GC-1 in association or not with GH for 4 weeks. Our findings show that T3 interacts with GH to promote body growth, differentiation of growth plate hypertrofic chondrocytes, intramembranous ossification of cranial bone, and increased of bone resistance and other biomechanics parameters that contribute to the best bone quality. On the other hand, ours results suggest strongly that TH acts in bone mass acquisition, in organization of growth plate chondrocytes and endocondral ossification mainly independent of GH/IGF-I axis and via TR<font face=\"symbol\">a and/or TR<font face=\"symbol\">b.

Sabe-se que o hipotireoidismo (Hipo) resulta em supressão do eixo hormônio de crescimento (GH)/ insulin-like growth factor I (IGF-I) e em atraso no desenvolvimento esquelético. Em um estudo anterior, vimos que o tratamento de ratas jovens Hipo com GC-1, um análogo da triiodotironina (T3) seletivo pela isoforma <font face=\"symbol\">b de receptor de hormônio tireoideano (TR<font face=\"symbol\">b), não teve efeito sobre o IGF-I sérico ou sobre a expressão protéica de IGF-I nas lâminas epifisiais, mas parcialmente reverteu alterações esqueléticas decorrentes do Hipo, o que sugere que: (i) o desenvolvimento esquelético requer ações do T3 mediadas pelo TR<font face=\"symbol\">a1 e TR<font face=\"symbol\">b1 (isoformas de TR expressas no osso); ou (ii) requer interações entre o eixo GH/IGF-I e o hormônio tireoideano. Neste estudo, investigamos essas hipóteses tratando ratas recém desmamadas Hipo com T3 ou GC-1 em associação ou não com o GH por 4 semanas. Os nossos achados mostram que o T3 e GH interagem para promover o desenvolvimento ósseo, mas que uma série de efeitos do T3 nesse processo independe do eixo GH/IGF-I e são mediadas pelo TR<font face=\"symbol\">a e/ou TR<font face=\"symbol\">b. / Thyroid hormone (TH) has important effects on bone development and metabolism. It is known that triiodotyronine (T3) has indirect actions in the skeleton through its influence on the production and secretion of growth hormone (GH)/ insulin-like growth factor (IGF-I) and/or other factors. On the other hand, direct actions of T3 on bone are recognized but not yet clear. Most of T3 action is mediaded by its nuclear receptors (TRs). TR<font face=\"symbol\">a1, TR<font face=\"symbol\">b1 e TR<font face=\"symbol\">b2 bind T3, while TR<font face=\"symbol\">a2 does not bind T3 and acts as an antagonist of genic transcription of TR<font face=\"symbol\">a1 and TR<font face=\"symbol\">b1. All these receptors, except TR<font face=\"symbol\">b2, are expressed in chondrocytes of growth plate, osteoblasts and osteoclastos. However, the functional roles of each TR isoformas in the bone development are incompletely understood. A few years, it is development GC-1, a synthetic analog of T3 which is selectivwe for TR<font face=\"symbol\">b1 over TR<font face=\"symbol\">a1. In recent study, we showed that treatment of hypothyroid young rats with T3 revert the IGF-I deficiency and skeleton defects caused by hypothyroidism. Since GC-1 treatment does not effects on serum levels of IGF-I or protein expression of IGF-I in the growth plate, but revert some bone alterations induced by T3 deficiency. Considering the selectivity of GC-1 for TR<font face=\"symbol\">b, these findings suggest that T3 has effects on bone development that are mediated by TR<font face=\"symbol\">b and independent of GH/IGF-I axis. On the other hand, the inability of GC-1 in completely revert the alterations of bone development suggests that the normal skeleton development require (i) T3 actions mediated by TR<font face=\"symbol\">a1 and TR<font face=\"symbol\">b1, or (ii) synergic or additive actions between GH/IGF-I axis and thyroid hormone. To investigate these hypotheses, 21 day-old hypothyroid female rats were treated with T3 or GC-1 in association or not with GH for 4 weeks. Our findings show that T3 interacts with GH to promote body growth, differentiation of growth plate hypertrofic chondrocytes, intramembranous ossification of cranial bone, and increased of bone resistance and other biomechanics parameters that contribute to the best bone quality. On the other hand, ours results suggest strongly that TH acts in bone mass acquisition, in organization of growth plate chondrocytes and endocondral ossification mainly independent of GH/IGF-I axis and via TR<font face=\"symbol\">a and/or TR<font face=\"symbol\">b.