Full-fat soybeans require heat treatment to denature trypsin inhibitors which interfere with proper digestion of the soybean protein. This study examines the heat treatment of soybeans in a continuous particulate-medium conduction processor. / Whole soybeans at 13% and 23% moisture were roasted at salt temperatures of 225, 250 and 275$ sp circ$C for residence times of 15, 30 and 60 seconds. The soybeans reached temperatures ranging from 107$ sp circ$C to 134$ sp circ$C, after which they were maintained in an insulated container for 0, 5, 10 or 15 minutes. Salt temperature, residence time, and moisture content were found to significantly affect final temperature, temperature change, moisture loss and total heat transfer. Moisture losses in the processor were very high, indicating that combining the processes of heat treatment and drying may provide a high combined efficiency. / Analysis of trypsin inhibitor activity and soluble protein indicated that all three factors likely affect soybean quality, and the soybean quality achieved in these tests is likely to be sufficient for livestock feeding, although this remains to be confirmed through feeding trials.

Levels of soybean trypsin inhibitors (SBTI) in soy-based infant formulas were measured and found to range from 3 to 28% of the activity measured in raw soybeans, with higher activity usually present in ready-to-feed compared to concentrate or powder formulations. Experiments were conducted to examine the influence of dietary SBTI on growth, serum enzyme, lipid and free amino acids and hepatic S-adenosylmethionine (SAM) status in weanling male rats. Diets containing graded amounts of SBTI were fed with and without the methionine antagonist ethionine. Changes in growth, serum enzymes, lipid and amino acid parameters in rats fed SBTI or ethionine indicated lipotrope deficit and compromise of the transsulfuration pathway. The combination of SBTI and ethionine exacerbated many of the symptoms and methyl donor deficit was indicated by hepatic SAM status. Methionine supplementation of SBTI + ethionine diets was beneficial at moderating changes while cysteine supplementation was not.

Thesis (Ph. D.)--Columbia University, 1923. / Vita. Bibliography: p. 24.

Thesis (M.S.)--Florida State University, 2004. / Advisors: Dr. Wei-Chun Chin and Dr. George Bates, Florida State University, College of Arts and Sciences, Dept. of Biological Science. Title and description from dissertation home page (viewed Sept. 23, 2004). Includes bibliographical references.

Bielefeld, Universiẗat, Diss., 2000.

It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved in thrombocyte activation via PAR2 during gill bleeding. In this study, I investigated another role of the trypsin that is secreted from zebrafish. This investigation has demonstrated a novel role of trypsin in zebrafish. Not only did this investigation demonstrate the role of trypsin in zebrafish behavior, but also it showed that PAR2 might be the receptor that is involved in trypsin-mediated behavioral response. In addition, we have shown that Gq and ERK inhibitors are able to block the trypsin pathway and prevent the escaping behavior. Finally, the results of this investigation suggest that the cells that respond to trypsin are surface cells, which have an appearance similar to that of neuromast cells.

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