No description available.

No description available.

No description available.

No description available.

No description available.

Thesis (MScAgric (Conservation Ecology and Entomology))--Stellenbosch University, 2011 / ENGLISH ABSTRACT: Climate change will alter both temperature and moisture availability in the future and therefore will likely affect vector borne disease prevalence. Organisms faced with changes in weather can respond in a variety of ways and this complicates any predictions and inferences for these organisms with climate change. Cause-and-effect links between climate change, insect vector responses, and changes in risk of disease transmission are poorly established for most vector borne diseases. Tsetse (Diptera, Glossinidae) are important vectors of trypanosome parasites posing a major threat to human health and socio-economic welfare in Africa. Water balance plays an important role in determining activity patterns, energy budgets, survival and population dynamics and, hence, geographic distribution and abundance of insects. Glossina species occupy a wide range of habitats in Africa and are notable for their desiccation resistance in xeric environments. Yet, whether or not the different species, subgroups or ecotype groups differ in susceptibility to changes in weather remain undetermined. The first main focus of my thesis was to test the effects of climate change on water balance traits (water loss rate, body water content and body lipid content) of adult tsetse flies. Four species from xeric and mesic habitats were exposed to a range of temperature (20 – 30 °C) and relative humidity (0 – 99 %) combinations. Water loss rates were significantly affected by measurement treatments, while body water content, body lipid content and mass were less affected and less variable across treatment combinations. The results provide support for mass-independent inter- and intra-specific variation in water loss rate and survival times. Therefore, water balance responses to variation in temperature and relative humidity are complex in Glossina, and this response varies within and among species, sub-groups and ecotypes in terms of magnitude and the direction of effect change. Secondly, I apply a mechanistic distribution model for G. pallidipes to predict potential population responses to climate change. I validate the mechanistic model (NicheMapperTM) results spatially and temporally using two methods. Both tests of the model showed that NicheMapper‟s predicted resting metabolic rate has great potential to capture various aspects of population dynamics and biogeography in G. pallidipes. Furthermore, I simulate the effect of phenotypic plasticity under different climate change scenarios and solve for the basic reproductive number of the trypanosomiasis disease (R0) under a future climate scenario. This integrated thesis provides strong evidence for a general decrease in optimal habitat for G. pallidipes under future climate change scenarios. However, it also provides strong support for a 1.85 fold increase in R0 based on changes in biting frequency as a result of higher predicted metabolic rates in the future. This might suggest that the reduction in optimal habitat could be outweighed by the increase in R0. The results demonstrate that an understanding of the physiological mechanism(s) influencing vectors of disease with climate change can provide insight into forecasting variation in vector abundance and disease risk. / AFRIKAANSE OPSOMMING: Die invloed van klimaatsverandering op die temperatuur en vog beskikbaarheid mag moontlik insek-oordraagbare siektes in the toekoms beïnvloed. Organismes wat verandering in klimaat ervaar kan op verskillende maniere reageer en daarom is voorspelling en afleidings van die reaksies op klimaatsverandering nie eenvoudig nie. Boonop is die verband tussen klimaatsverandering, insek reaksies en veranderinge in die oordragsrisiko van siektes onbekend vir die meeste insekoordraagbare siektes. Tsetse (Diptera: Glossinidae) is belangrike draers van trypanosoom parasiete wat 'n bedreiging inhou vir mensegesondheid en sosio-ekonomiese welsyn in Afrika. Waterbalans speel 'n belangrike rol in die energiebondel samestelling, aktiwiteitspatrone, oorlewing en populasie dinamika van insekte en, dus, die geografiese voorkoms en verspreiding van insekte. Glossina spesies kom in 'n verskeidenheid habitatte in Afrika voor en is bekend daarvoor dat hulle weerstand bied teen uitdroging in droё habitatte. Maar, die mate waartoe die verskillende subgroepe, ekotiepegroepe en spesies kwesbaar is vir klimaatsverandering, is steeds onbekend. Die eerste hooffokus van my tesis was om die uitwerking van klimaatsverandering op waterbalansrelevante uitkomste (tempo van waterverlies, waterinhoud en vetinhoud) van volwasse tsetse vlieё te bestudeer. Vier spesies van droë en klam habitatte is aan verskillende kombinasies van temperatuur (20 – 30 °C) en relatiewe humiditeit (0 – 99 %) blootgestel. Die tempo van waterverlies is betekenisvol deur die verskillende toetskombinasies beïnvloed, terwyl die waterinhoud, vetinhoud en liggaamsmassa tot 'n minder mate beïnvloed is en minder gevarieer het tussen die toetskombinasies. Die resultate toon bewyse vir gewigs-onafhanklike inter- en intraspesie variasie in waterverlies tempo‟s en oorlewingstyd. Die waterbalans uitkomste op variasie in temperatuur en relatiewe humiditeit is dus ingewikkeld in Glossina, en dit varieer binne en tussen spesies, subgroepe en ekotiepe in terme van die graad en rigting van effek verandering. Tweedens pas ek 'n meganistiese verspreidingsmodel toe vir G. pallidipes om die moontlike populasiereaksies met klimaatsverandering te voorspel. Ek toets die antwoorde van die model (NicheMapperTM) oor tyd en skaal op twee verskillende maniere. Beide toetse het aangedui dat die NicheMapper voorspelde rustende metaboliese tempo die verskillende aspekte van G. pallidipes populasie dinamika en biogeografie goed beskryf. Ek simuleer die uitkomste van die fenotipiese veranderbaarheid van G. pallidipes onder „n verskeidenheid klimaatsverandering-uitkomste, en los „n model van die basiese ommekeer van trypanosomiasis (R0) op onder 'n klimatsverandering situasie in die toekoms. Hierdie geïntegreerde tesis toon sterk bewyse dat die optimale habitat van G. pallidipes verminder met klimaatsverandering. Dit toon egter ook bewyse vir 'n 1.85 keer toename in R0 gebasseer op 'n verhoging in die frekwensie van bytgeleenthede weens die hoër voorspelde metaboliese tempo van die vlieë in die toekoms. Laasgenoemde stel voor dat die afname in optimale habitat moontlik deur 'n toename in R0 oorheers sal word. Die resultate demonstreer dat beter begrip van die fisiologiese meganisme(s) wat parasiet-draers beïnvloed verdere insig kan voorsien in die toekomstige voorspelling van draer teenwoordigheid en siekte waarskynlikheid.

In October 2006 an intervention was initiated to arrest the northerly advance through Uganda of the zoonotic parasite Trypanosoma brucei rhodesiense. This is a protozoal infection that is vectored by the tsetse fly. It is the aim of this thesis to review the impact of this large scale treatment programme in terms of animal health and human disease. The Stamp Out Sleeping Sickness (SOS) campaign was designed to target the cattle reservoir of T. b. rhodesiense in these newly affected areas by block treating >180,000 head of cattle. This was achieved in collaboration with final year vet students from the University of Makerere, Uganda. Farmers were also encouraged to spray their animals with deltamethrin in order to suppress the tsetse population. In order to monitor the impact of this intervention a base line survey was carried out. Evaluation of the logistics and implementation of the SOS campaign was assessed through interviews with personnel involved. Analysis by PCR revealed the prevalence of T. brucei s.l. as 15.57% (T. b. rhodesiense as 0.81%) within the cattle reservoir prior to SOS treatment. Follow up sampling was carried out at 23 locations at three, nine and 18 months. The prevalence of T. brucei s.l. was reduced post treatment, but in the absence of sustained vector control infections amongst the animals returned by nine months and subsequently exceeded the base line findings (P=<0.0001). It was observed that across most of the SOS area, T. b. rhodesiense did not re-establish following treatment. However, a significant cluster was identified where cases of both human and animal disease were continually reported. This cluster was noted to include the area immediately surrounding the Otuboi cattle market. This link between cattle movement and the spread of T. b. rhodesiense is an established one and is addressed by Ugandan governmental policy which states that ‘cattle traded at market must be treated with trypanocidal drugs prior to movement’. The findings presented here suggest that this policy may not be strictly enforced. The risk of spread is compounded at the northern districts of Uganda restock their domestic livestock following years of civil conflict. The majority of animals are traded in a northward direction – transporting infected animals from the endemic south. The scale of this trade is assessed through questionnaires, analysis of trade records and animal screening. Specific consideration is given to the implications of this cattle trade and impact this may have on the sustainability of the SOS campaign.

Trypanosoma brucei is a protozoan parasite that is the causative agent of sleeping sickness in sub-Saharan Africa. T. brucei has a complex life cycle involving passage between a mammalian host and the tsetse fly. The parasite evades the mammalian immune system via expression of Variant Surface Glycoprotein (VSG) on the cell surface. VSG genes are expressed at telomeric expression sites and at these sites are a number of Expression Site Associated Genes (ESAGs). One unusual ESAG, ESAG9, is developmentally regulated: RNA for these genes accumulates during the transition from slender to stumpy cells in the mammalian bloodstream and cellassociated protein is only detected transiently in stumpy and differentiating cells. Transgenic cell lines were generated which ectopically express one or more members of the ESAG9 gene family. Biochemical and cytological analyses using these cell lines indicated that some members of this family are glycosylated and GPI-anchored, and also that one gene, ESAG9-K69, is secreted. ESAG9-K69 is also secreted by wild-type stumpy parasites. In vivo experiments with tsetse flies did not conclusively show whether ESAG9 proteins play a role in the establishment of a tsetse fly mid-gut infection by transgenic trypanosomes. However, In vivo and ex vivo experiments using the mouse model of trypanosomiasis indicated that expression of ESAG9 proteins may alter parasitaemia in the mouse and results in a significant decrease in the proportion of CD4+ T cells in the mouse spleen.

Please read the abstract in the section 00front of this document / Thesis (PhD (Entomology))--University of Pretoria, 2005. / Zoology and Entomology / unrestricted

Os tripanosomas são parasitas hemoflagelados com importância médica para humanos e animais domésticos. Surtos de infecções agudas graves por T.vivax têm sido relatados em todo Brasil onde perdas econômicas e falhas reprodutivas são observadas. Neste trabalho, uma infecção experimental por T. vivax em ovinos confirmou sua transmissão transplacentária. Em relação à epidemiologia de T. vivax, para entender o papel dos jumentos em surtos no Semiárido Brasileiro, foram realizados ensaios parasitológicos e moleculares em infecções naturais e experimentais. Nossos resultados mostraram animais assintomáticos, portanto, podem atuar como potenciais reservatórios. Além disso, estudos filogenéticos revelaram uma diversidade genética inesperada em tripanossomas no Leste da África, especialmente em moscas tsé-tsé. Novos genótipos de T. vivax foram caracterizados e tripanossomas do subgênero Nanomonnas se mostraram prevalentes nestas infecções. Nesta abordagem, conseguimos identificar T. suis e novas espécies do subgênero Pycnomonas presentes em animais selvagens e domésticos. / Trypanosomes are hemoflagellates parasites with medical importance to humans and domestic animals. Outbreaks of severe acute infections by T. vivax have been reported throughout Brazil, where economic loss and reproductive failure are observed. In this study, an experimental infection by T. vivax in sheep confirmed the transplacental transmission of this parasite. Regarding T. vivax epidemiology, to understand the role played by donkeys in outbreaks in the Brazilian Semiarid, we performed parasitological and molecular assays in naturally and experimentally infections. Our findings show that infected donkeys are asymptomatic and, thus, are potential reservoirs. In addition, phylogenetic studies have revealed an unexpected genetic diversity in East Africa, especially in tsetse flies. New genotypes of T. vivax were characterized and trypanosomes of the subgenus Nanomonnas were prevalent in these infections. In this approach, we can identify T. suis and new species of the subgenus Pycnomonas present in wild and domestic animals.