Spelling suggestions: "subject:"tumor microenvironment"" "subject:"tumor microenvironments""
121 |
PLOD Family: A Novel Biomarker for Prognosis and Personalized Treatment in Soft Tissue SarcomaGong, Siming, Schopow, Nikolas, Duan, Yingjuan, Wu, Changwu, Kallendrusch, Sonja, Osterhoff, Georg 09 June 2023 (has links)
Despite various treatment attempts, the heterogenous group of soft tissue sarcomata (STS) with more than 100 subtypes still shows poor outcomes. Therefore, effective biomarkers for prognosis prediction and personalized treatment are of high importance. The Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase (PLOD) gene family, which is related to multiple cancer entities, consists of three members which encode important enzymes for the formation of connective tissue. The relation to STS, however, has not yet been explored. In this study, data from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to analyze the role of PLOD1–3 in STS. It was found that an overexpression of PLOD family members correlates with poor prognosis, which might be due to an increased infiltration of immune-related cells in the tumor microenvironment. In STS, the expression of PLOD genes could be a novel biomarker for prognosis and a personalized, more aggressive treatment in these patients.
|
122 |
Über das Expressionsverhalten von Reparatur- und ABC- Transporter-Genen sowie inflammatorischen Signalwegen im Kolon- und Pankreaskarzinom / Expression of heatshock proteins, ABC-transporters and toll-like transporters under nutrient deprivation in a colorectal and pancreatic tumor modelSchmitt, Johannes Christian January 2021 (has links) (PDF)
Das Mikromilieu solider Tumor (tumor mircoenvironment, TME) weist verschiedene Besonderheiten auf, von denen bekannt ist, dass sie zu Chemotherapieresistenz und Tumorprogression beitragen können. Neben der Extrazellulären Matrix (ECM), den cancer associated cells (CAC) und diversen Entzündungszellen tragen auch chemische und physikalische Besonderheiten (Hypoxie, Azidose, erhöhter Gewebedruck, oxidativer Stress und Nährstoffmangel) zu Tumorprogression und Chemotherapieresistenz bei. Zudem wissen wir, dass Hitzeschock-Proteine (HSPs), Toll-like Rezeptoren (TLRs) und ABC-Transporter mit erhöhter Chemotherapieresistenz und Tumorprogression im Pankreas- und Kolonkarzinom einhergehen.
Hier wurde untersucht, ob ein in vitro induzierter Nährstoffmangel im HT29 Kolonkarzinom, im Panc-1 Pankreaskarzinom und im MIA PaCa-2 Pankreaskarzinom zu einer gesteigerten Expression von HSP70, HSP90, MDR1, ABCB5 und TLR1 bis TLR10 auf mRNA und Proteinebene führt. Zudem wurde unter allen Versuchsbedingungen die Stoffwechselaktivität über einen MTS-Test gemessen. Der Nährstoffmangel wurde über die Kultivierung in einem Hybridomamedium, welches als proteinfreies Medium gilt und über die Kultivierung in einem serumfreien Medium induziert.
Es zeigte sich, dass insbesondere die entdifferenzierte Panc-1 Pankreaskarzinomzelllinie eine erhöhte Resistenz gegenüber dem induzierten Nährstoffmangel aufwies. Auf mRNA-Ebene zeigten sich bei allen drei Tumorzelllinien deutliche Expressionssteigerungen. Diese waren insbesondere im Hybridomamedium nachweisbar und traten beim HT29-Kolonkarzinom nach 48h und im Panc-1 Pankreaskarzinom bereits nach 24h auf. Besonders intensive Expressionssteigerungen konnten im HT29 Kolonkarzinom bei ABCB5, TLR7 und TLR9 nachgewiesen werden. Die Expression von MDR1 war insbesondere im MIA PaCa-2 Pankreaskarzinom gesteigert. Auf Proteinebene konnte im HT29 Kolonkarzinom eine Expressionssteigerung bei HSP90 und TLR6 nachgewiesen werden.
Die Ergebnisse lassen zwei Interpretationen zu. Zum einen könnte über den Nährstoffmangel eine aggressivere Subpopulation selektioniert worden sein. In diesem Zusammenhang konnten die Expressionsdaten des Tumorstammzellmarkers CD133 leider nicht ausgewertet werden. Alternativ kann angenommen werden, dass die untersuchten Tumorzelllinien ihren aggressiven Phänotyp erst unter Nährstoffmangelbedingungen, wie wir sie regelmäßig in soliden Tumoren finden, zur Expression bringen. / The tumor microenvironment (TME) in solid tumors is low on nutrients and favors tumor progression and resistance to chemotherapies in different ways.
In this study we cultured HT29 colorectal carcinoma cells, Panc-1 pancreatic carcinoma cells and MIA PaCa-2 pancreatic carcinoma cells in nutrient deprived conditions (NDC) and performed rtPCR expression analysis, SDS-PAGE and immunohistochemical staining after 24, 48 and 72 hours. Gene expression of ABC transporters (ABCB5, MDR1), heat-shock proteins (HSP70, HSP90) and Toll-like receptors (TLR1 – TLR10) in the NDC compared to normal condition was analyzed. We performed MTS tetrazolium assays to monitor the activity of the respiratory chain in any condition.
We showed that the examined cell lines, and in particular Panc-1 pancreatic carcinoma, are very resistant to the NDC. The gens of interest showed increase expression after 48 hours (HT29) and 24 hours (Panc-1).
The results suggest that culturing in NDC either selects a very aggressive and resistant subpopulation or NDC induces gene expression changes and shows us how cancer cells really perform in the nutrient deprived tumor environment. Unfortunately, we were not able to use the gene expression analysis of the stem cell marker CD133.
|
123 |
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase Family: Novel Prognostic Biomarkers and Tumor Microenvironment Regulators for Lower-Grade GliomaGong, Siming, Wu, Changwu, Köhler, Franziska, Meixensberger, Jürgen, Schopow, Nikolas, Kallendrusch, Sonja 05 April 2023 (has links)
Lower-grade glioma (LGG) is a group of tumors arising from the cells of the central
nervous system. Although various therapy interventions are used, the prognosis
remains different. Novel biomarkers are needed for the prognosis of disease and novel
therapeutic strategies in LGG. The procollagen-lysine, 2-oxoglutarate 5-dioxygenase
(PLOD) family contains three members and is related to multiple cancers, yet it was
not investigated in LGG. Data from the Chinese Glioma Genome Atlas (CGGA) and
The Cancer Genome Atlas (TCGA) cohorts were used to analyze the role of PLOD in
LGG. As the PLOD family is involved in processes, such as tumor formation and cancer
metastasis, we focused on its relationship to the tumor microenvironment (TME) in LGG.
A high expression of the PLOD family relates to poor prognosis and high infiltration of
immune cells within the TME. The expression level of the PLOD family might become a
novel biomarker for prognosis and is a potential target for individual treatment decisions
in LGG.
|
124 |
Cancer Signals-Triggered T Cell Immunotherapy for Solid TumorsNguyen, Huong Thi Xuan January 2022 (has links)
No description available.
|
125 |
Epithelial and Macrophage RON Receptor Signaling Regulates the Antitumor Immune Response in Prostate CancerSullivan, Camille 22 October 2020 (has links)
No description available.
|
126 |
Multielectrode platform for measuring oxygenation status in multicellular tumor spheroidsSheth, Disha B. 25 April 2011 (has links)
No description available.
|
127 |
Biomimetic Electrospun Fibers for Cancer Cell Migration, Chemotaxis, andAnti-Metastatic Drug TestingNelson, Mark Tyler 26 May 2015 (has links)
No description available.
|
128 |
Cell Proliferation Control: from Intrinsic Transcriptional Programs to Extrinsic Stromal NetworksLiu, Huayang 14 August 2015 (has links)
No description available.
|
129 |
Engineering Tumor Models Using Aqueous Biphasic 3D Culture MicrotechnologyHam, Stephanie Lemmo January 2017 (has links)
No description available.
|
130 |
Immuno-nanotherapeutics to Inhibit Macrophage Polarization for Non-Small-Cell Lung CancersSeshadri, Dhruv Ramakrishna January 2017 (has links)
No description available.
|
Page generated in 0.1024 seconds