Thesis (M.Med.Sc.)--University of Western Australia, 2007.

Thesis (M.S.)--West Virginia University, 2004. / Title from document title page. Document formatted into pages; contains iv, 27 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 26-27).

Thesis (MSc. (Companion Animal Clinical Studies))--University of Pretoria, 2000. / Also available in print format.

Thesis (doctoral)--Königsberg i. Pr., 1901.

Thesis (Ph.D.)--University of Nebraska-Lincoln, 2007. / Title from title screen (site viewed Oct. 10, 2007). PDF text: 128 p. UMI publication number: AAT 3258736. Includes bibliographical references. Also available in microfilm and microfiche formats.

Proefschrift Universiteit Maastricht. / Met lit. opg. - Met samenvatting in het Nederlands.

Made available in DSpace on 2014-06-11T19:23:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-23Bitstream added on 2014-06-13T19:50:46Z : No. of bitstreams: 1 pereira_ml_me_jabo.pdf: 321454 bytes, checksum: e1f70686b52e5be19e89ddd3396d5e7e (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Os glicosaminoglicanos (GAGs) são mucopolissacarídeos eliminados normalmente na urina. A alteração da excreção dessas substâncias está relacionada à ocorrência de doenças sistêmicas ou do trato urinário. Dentre elas, a cistite intersticial, cuja fisiopatogenia ainda não foi esclarecida, acomete seres humanos, e os felinos, por apresentarem quadro semelhante, têm sido considerados o melhor modelo para estudo desta doença. Considerando que os GAGs podem estar envolvidos nos mecanismos da enfermidade, é importante a padronização de métodos para dosagem destes mucopolissacarídeos em urina de felinos, bem como o estabelecimento de valores de referência para a espécie. Foram avaliados 12 felinos sadios, dos 30 aos 114 dias de idade. Amostras de urina foram coletadas a cada 28 dias para quantificação de GAGs sulfatados. Para as mensurações, foram avaliados quatro métodos colorimétricos em busca do mais sensível, barato e reprodutível. Dentre eles, o de desempenho melhor foi o que emprega azul de dimetilmetileno (DMB) em tampão acetato sódico, padrão com sulfato de condroitina C (SCC) após modificação do volume de urina empregado na reação, do tempo de leitura dos testes, além da dispensa da centrifugação das amostras de urina. A excreção urinária de GAG sulfatado não foi influenciada pelo sexo, mas foi modificada pelo fator idade. Os valores foram significativamente mais altos aos 30 e aos 58 dias de idade em relação aos observados para as idades subseqüentes (86 e 114 dias), revelando declínio das quantidades desta substância na urina de felinos desta faixa etária. / Glycosaminoglycans (GAGs) are mucopolisacarides usually eliminated in urine. The altered excretion of these substances is related to occurrence of systemic or urinary diseases. Among them, interstitial cystitis, which physiopatogenesis has not been elucidated yet, is observed mainly in humans, and felines, that present similar signs, have been considered the best model for study of this illness. Considering that GAGs may be involved with disease mechanisms, the standardization of methods to measure mucopolissacarides in feline urine, as the establishment of reference values for the species, are very important. Twelve healthy kittens were studied, from 30 to 114 days old, and specimens of urine were collected every 28 days for quantification of GAGs. Four colorimetric methods were analyzed, in order to find the most sensible, cheap and reproducible for dosage. The selected method, that employs dimethylmethylene blue (DMB) with sodium acetate buffer and standard with chondroitin C sulfate, was modified with regard to the volume of urine employed, reading time of the tests, and dispense of urine centrifugation. Values were significantly higher at ages 30 and 58 days old when compared to those observed at subsequent ages (86 and 114 days), revealing decline of these substance quantities in feline urine on this age interval.

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Polyhalite is a natural mineral that could be fed as an acidogenic product to induce a metabolic acidosis and prevent clinical hypocalcemia in dairy cows after calving. The overall objective of this study was to determine if the use of polyhalite mineral in the diets of pre-partum non-lactating dairy cows was effective as an acidogenic product. We measured the urine pH, dry matter intake, milk yield, and calcium and magnesium concentration of urine and serum in pre-partum and non-lactating dairy cows consuming diets containing a low dose of polyhalite (200 g/cow/day), a high dose of polyhalite (400 g/cow/day), calcium chloride (250 g/cow/day), or no acidogenic product. We hypothesized that including polyhalite mineral as an acidogenic product in the diets of pre-partum and non-lactating dairy cows will reduce urine pH and stimulate calcium metabolism mechanisms. We found that polyhalite effectively reduced urine pH and did not affect dry matter intake, and the stimulation of calcium metabolism was observed through an increase of calcium output in urine. In conclusion, feeding polyhalite mineral is an effective means for inducing metabolic acidosis without reducing dry matter intake. Based on these results, polyhalite should be fed at a dose of 400 g or more per cow per day to reduce urine pH. / Master of Science in Life Sciences / Low blood calcium concentration, also known as hypocalcemia, is one of the common metabolic disorders that affect dairy cows transitioning from the pre-partum to post-partum period. Reducing the dietary cation anion difference (DCAD) in cows during the close-up period is known to effectively reduce the probability of cows developing hypocalcemia after calving. Polyhalite is a natural mineral that could be fed as an acidogenic product to induce a metabolic acidosis and prevent hypocalcemia in dairy cows after calving. The overall objective of this study was to determine if the use of polyhalite mineral in the diets of pre-partum and non-lactating dairy cows was effective as an acidogenic product. We evaluated the urine pH, dry matter intake, milk yield, and calcium and magnesium concentration of urine and serum in pre-partum and non-lactating dairy cows consuming diets containing a low dose of polyhalite (200 g/cow/day) , a high dose of polyhalite (400 g/cow/day), calcium chloride (250 g/cow/day), or no acidogenic product. We hypothesized that including polyhalite mineral as an acidogenic product in the diets of pre-partum and non-lactating dairy cows will reduce urine pH and stimulate calcium metabolism mechanisms. We found that polyhalite effectively reduced urine pH and did not affect dry matter intake, and the stimulation of calcium metabolism was observed through an increase of calcium output in urine. In conclusion, feeding polyhalite mineral is an effective means for inducing metabolic acidosis without reducing dry matter intake. Based on these results, polyhalite should be fed at a dose of 400 g or more per cow per day to reduce urine pH.