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Topologically conserved hydrophobic residues of the thioredoxin C-subdomain stabilise GSTsParbhoo, Nishal 12 June 2014 (has links)
The thioredoxin-like fold is a well conserved fold that is present in many families of proteins. One such superfamily of proteins include the GSTs which are involved in phase II detoxification. GSTs primarily catalyse the metabolism of xenobiotics but are also involved in transporting non–substrate ligands and reactive compounds. The GST fold comprises an N-terminal thioredoxin domain and an all alpha helical C-terminal domain and is present in at least 18 classes of proteins. The N-terminal thioredoxin domain is characterised by the βαβαββα topology and can be further divided into two structural motifs, an N-terminal (βαβ) and a C-terminal (αββα) motif. A well conserved hydrophobic network exists between these two motifs and the role of the C-terminal motif is elucidated in this study using class Alpha GST as a model protein. A topologically conserved valine (Val58) and an isoleucine (Ile75) located on β3 and α3, respectively, were mutated to alanine. Secondary and tertiary structural characterisation as well as ligandin function of the mutant enzymes displayed no major structural alteration with respect to the wild-type enzyme. This was confirmed with high resolution crystal structures obtained. Enzymatic activity was maintained indicating that no structural alterations have occured that affects the active site dynamics and the domain interface as a result of the induced mutations. Thermal denaturation studies, however, indicated a slight destabilisation in the enzyme in the case of the valine mutation, but a large destabilisation was witnessed as a result of the isoleucine mutation. This is further observed in denaturant-induced equilibrium studies where the thermodynamic stability of proteins can be determined. Furthermore, as a result of the isoleucine mutation, the enzyme unfolds via a populated intermediate in contrast to the wild-type which globally unfolds via a two-state mechanism with no stable intermediates being populated. Pulse-proteolysis was employed as an additional probe for thermodynamic stability where the enzyme was digested by thermolysin at varying denaturant concentrations. Pulse-proteolysis results were in agreement with the thermal and denaturant-induced stability studies further confirming that the isoleucince substitution causes a large destabilisation. Thus these conserved hydrophobic residues of the thioredoxin C-subdomain play a crucial stabilising role in the GST fold.
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Optikers möjlighet att bemöta konkurrens : Att styra value-added sellingDamm, Erik, Eliassi, Zana, Lorentzon, Jonas January 2013 (has links)
I denna uppsats har en studie genomförts med målet att ur ett företagsekonomiskt perspektiv beskriva och sedan analysera hur mindre optikerföretag styrs mot value added selling filosofin. Studien har genomförts med en abduktiv ansats och med en kvalitativ metod. Empiriskt material har samlats in via semi-strukturerade intervjuer med Hagelin & Co, Björkegrens Optik AB, Optiker Tottie och Specsavers. Vi författare har kommit fram till att value added selling filosofin finns mer eller mindre implementerat de flesta mindre företag i någon form av norm eller företagskultur. Det är också företagskulturen vi författare identifierar som det viktigaste styrmedlet för value-added selling. I optikerbranschen som vi studerat är detta speciellt intressant av den anledningen att respondenterna visat flera tecken på hur value added selling filosofin fungerar, vilket vi författare anser gör studien relevant för mindre företag i allmänhet som verkar på en konkurrensintensiv marknad.
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noneChen, Chi-Lin 17 June 2005 (has links)
none
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The value of economic reality applying Economic Value Added /Phillips, David M. January 2007 (has links)
Thesis (Honors)--Liberty University Honors Program, 2007.
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A critical analysis of the VAT Act amendments relevant to South African municipalitiesGranova, Olga. January 2009 (has links)
Thesis (M.Com.(Taxation))--University of Pretoria, 2009. / Abstract in English and Afrikaans. Includes bibliographical references.
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Die Mehrwertsteuer im Zivilrecht /Herbrig, Wolfgang. January 1900 (has links)
Thesis (doctoral)--Universität Berlin.
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EVA : an effective tool for measuring performance /McGorman, Jason. January 2007 (has links)
Thesis (Honors)--Liberty University Honors Program, 2007. / Includes bibliographical references. Also available through Liberty University's Digital Commons.
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The value of economic reality : applying Economic Value Added /Phillips, David. January 2007 (has links)
Thesis (Honors)--Liberty University Honors Program, 2007. / Includes bibliographical references. Also available through Liberty University's Digital Commons.
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Rechnungswesenbasierte Verfahren der Aktienbewertung : theoretische und empirische Untersuchung des Residualgewinnmodells /Koch, Joachim. January 2005 (has links) (PDF)
Zugl.: Vallendar, Univ., Diss., 2005.
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VAT compliance in small businesses : a social psychological perspectiveAdams, Caroline Jane January 2002 (has links)
No description available.
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